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New Tool for Per-Sequence Depths #62

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oschwengers opened this issue May 25, 2023 · 1 comment
Open

New Tool for Per-Sequence Depths #62

oschwengers opened this issue May 25, 2023 · 1 comment

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@oschwengers
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Hi @sstadick and thanks a lot for developing perbase!
I'd love to use perbase to estimate the final coverages of multisteps short/long/hybrid bacterial whole-genome assemblies. For these use cases, a distinct tool for per-sequence summarizing information would be very helpful.

For example if we could get the following information for each sequence within an assembly:

  • seq-id
  • min coverage
  • average coverage
  • max coverage
  • normalized cov

By "normalized cov" I mean the average coverage of a seq normalized to the average coverage of the longest sequence, like in Unicycler. By this we get important information, as for example that a sequence "B" has 5x the coverage of sequence "A" (often the longest sequence is the chromosome or at least a larger part of it).

Again thanks and best regards!

@sstadick
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sstadick commented Jun 8, 2023

Hi! I'll keep this open in the event that I come back into perbase to do more development, but I don't currently have enough bandwidth to be doing a lot of work here, PRs are always welcome though!

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