diff --git a/apis/python/setup.py b/apis/python/setup.py
index 9c5021400d..49285918e9 100644
--- a/apis/python/setup.py
+++ b/apis/python/setup.py
@@ -291,7 +291,7 @@ def run(self):
         "scanpy>=1.9.2",
         "scipy",
         "somacore==1.0.4",
-        "tiledb~=0.22.0",
+        "tiledb~=0.22.1",
         "typing-extensions",  # Note "-" even though `import typing_extensions`
     ],
     extras_require={
diff --git a/apis/r/tools/get_tarball.R b/apis/r/tools/get_tarball.R
index 97ab7f371e..9ce4d3337a 100644
--- a/apis/r/tools/get_tarball.R
+++ b/apis/r/tools/get_tarball.R
@@ -1,8 +1,8 @@
 #!/usr/bin/env Rscript
 
 ## version pinning info
-tiledb_core_version <- "2.16.0"
-tiledb_core_sha1 <- "d682dd0"
+tiledb_core_version <- "2.16.1"
+tiledb_core_sha1 <- "3abc68f"
 
 if ( ! dir.exists("inst/") ) {
     stop("No 'inst/' directory. Exiting.", call. = FALSE)
diff --git a/libtiledbsoma/cmake/Modules/FindTileDB_EP.cmake b/libtiledbsoma/cmake/Modules/FindTileDB_EP.cmake
index 07172299da..033b7a0b3f 100644
--- a/libtiledbsoma/cmake/Modules/FindTileDB_EP.cmake
+++ b/libtiledbsoma/cmake/Modules/FindTileDB_EP.cmake
@@ -58,8 +58,8 @@ else()
     # NB When updating the pinned URLs here, please also update in file apis/r/tools/get_tarball.R
     if(DOWNLOAD_TILEDB_PREBUILT)
         if (WIN32) # Windows
-          SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.0/tiledb-windows-x86_64-2.16.0-d682dd0.zip")
-          SET(DOWNLOAD_SHA1 "82a79fda3daecc46ae24c86a328e52184460dabe")
+          SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.1/tiledb-windows-x86_64-2.16.1-3abc68f.zip")
+          SET(DOWNLOAD_SHA1 "3bc7d9f88f67b1138dd23a4b99a22a7b16ebf74f")
         elseif(APPLE) # OSX
 
           # Status quo as of 2023-05-18:
@@ -76,22 +76,22 @@ else()
           #   o CMAKE_SYSTEM_PROCESSOR is x86_64
 
           if (CMAKE_OSX_ARCHITECTURES STREQUAL x86_64)
-            SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.0/tiledb-macos-x86_64-2.16.0-d682dd0.tar.gz")
-            SET(DOWNLOAD_SHA1 "d7b22fa3cb9cbe131734d9f18beb3362908aeccf")
+            SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.1/tiledb-macos-x86_64-2.16.1-3abc68f.tar.gz")
+            SET(DOWNLOAD_SHA1 "a0f322d7169635065e2117feac6cc1725e5c97cb")
           elseif (CMAKE_OSX_ARCHITECTURES STREQUAL arm64)
-            SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.0/tiledb-macos-arm64-2.16.0-d682dd0.tar.gz")
-            SET(DOWNLOAD_SHA1 "09c62e3211e5277263c1545d74d698d450ebcb77")
+            SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.1/tiledb-macos-arm64-2.16.1-3abc68f.tar.gz")
+            SET(DOWNLOAD_SHA1 "e2ad15187561c72169ad998183d8d65984ad0f03")
           elseif (CMAKE_SYSTEM_PROCESSOR MATCHES "(x86_64)|(AMD64|amd64)|(^i.86$)")
-            SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.0/tiledb-macos-x86_64-2.16.0-d682dd0.tar.gz")
-            SET(DOWNLOAD_SHA1 "d7b22fa3cb9cbe131734d9f18beb3362908aeccf")
+            SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.1/tiledb-macos-x86_64-2.16.1-3abc68f.tar.gz")
+            SET(DOWNLOAD_SHA1 "a0f322d7169635065e2117feac6cc1725e5c97cb")
           elseif (CMAKE_SYSTEM_PROCESSOR MATCHES "^aarch64" OR CMAKE_SYSTEM_PROCESSOR MATCHES "^arm")
-            SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.0/tiledb-macos-arm64-2.16.0-d682dd0.tar.gz")
-            SET(DOWNLOAD_SHA1 "09c62e3211e5277263c1545d74d698d450ebcb77")
+            SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.1/tiledb-macos-arm64-2.16.1-3abc68f.tar.gz")
+            SET(DOWNLOAD_SHA1 "e2ad15187561c72169ad998183d8d65984ad0f03")
           endif()
 
         else() # Linux
-          SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.0/tiledb-linux-x86_64-2.16.0-d682dd0.tar.gz")
-          SET(DOWNLOAD_SHA1 "be0bfd5512c159b960988a4bd41366e9f020115f")
+          SET(DOWNLOAD_URL "https://github.com/TileDB-Inc/TileDB/releases/download/2.16.1/tiledb-linux-x86_64-2.16.1-3abc68f.tar.gz")
+          SET(DOWNLOAD_SHA1 "0d65d8a1c0766dec3a5a76e72419850b76c955b7")
         endif()
 
         ExternalProject_Add(ep_tiledb
@@ -113,8 +113,8 @@ else()
     else() # Build from source
         ExternalProject_Add(ep_tiledb
           PREFIX "externals"
-          URL "https://github.com/TileDB-Inc/TileDB/archive/2.16.0.zip"
-          URL_HASH SHA1=c2779f931c84d931e5fca6dc3a858e66fc542276
+          URL "https://github.com/TileDB-Inc/TileDB/archive/2.16.1.zip"
+          URL_HASH SHA1=da077ef3970a3e48a2bb28f35c45d5775b6f7c9e
           DOWNLOAD_NAME "tiledb.zip"
           CMAKE_ARGS
             -DCMAKE_INSTALL_PREFIX=${EP_INSTALL_PREFIX}