From d3da264c21384311fd0b6e2f18e9a092c5e19e6a Mon Sep 17 00:00:00 2001 From: Sam El-Kamand Date: Mon, 5 Aug 2024 13:27:42 +1000 Subject: [PATCH 1/2] docs: data source acknowledgement and minor revisions --- paper/jats/paper.jats | 29 ++++++++++++++++++----------- paper/paper.bib | 34 ++++++++++++++++++++++++++-------- paper/paper.md | 11 ++++++----- paper/paper.pdf | Bin 725422 -> 727028 bytes 4 files changed, 50 insertions(+), 24 deletions(-) diff --git a/paper/jats/paper.jats b/paper/jats/paper.jats index c7bef55..5597371 100644 --- a/paper/jats/paper.jats +++ b/paper/jats/paper.jats @@ -18,8 +18,8 @@ 0 N/A -ggoncoplot: an R package for visualising somatic mutation -data from cancer patient cohorts +ggoncoplot: an R package for interactive visualisation of +somatic mutation data from cancer patient cohorts @@ -105,9 +105,9 @@ a Creative Commons Attribution 4.0 International License (CC BY types, including gene expression t-SNE plots or methylation UMAPs. The simplest and most intuitive approach to examining such relations is to link plots dynamically such that samples selected in an oncoplot can - be highlighted in other plots. There are, however, no existing - oncoplot-generating R packages that support dynamic data linkage - between different plots. To address this gap and enable rapid + be highlighted in other plots, and vice versa. There are, however, no + existing oncoplot-generating R packages that support dynamic data + linkage between different plots. To address this gap and enable rapid exploration of a variety of data types we constructed the ggoncoplot package for the production of oncoplots that are easily integrated with custom visualisations and that support synchronised @@ -140,12 +140,16 @@ a Creative Commons Attribution 4.0 International License (CC BY samples were automatically highlighted on the UMAP and oncoplot. This reveals that samples which cluster on the left of the t-SNE plot also cluster in the oncoplot, chiefly containing mutations in - TP53 but wild type PIK3CA. The plots of progesterone, estrogen, HER2 + TP53 and wild type PIK3CA. The plots of progesterone, estrogen, HER2 status and triple negative classification show that the samples - selected in the t-SNE are virtually all triple negative breast + selected in the t-SNE are enriched for triple negative breast cancers. In contrast to the oncoplot, the methylation UMAP shows no - strong clustering, in line with knowledge of methylation patterns in - triple negative breast cancer. + strong clustering, consistent with knowledge of methylation patterns + in triple negative breast cancer. Methylation, expression, and + genome data were originally obtained from the UCSC Xena TCGA + Pan-Cancer Atlas Hub [goldman:2020]. Expression and methylation + plots were produced with + express.

@@ -182,8 +186,8 @@ a Creative Commons Attribution 4.0 International License (CC BY visualised in an oncoplot.

-

Auto-colouring: Automatic selection of colour - palettes for datasets where the consequence annotations are +

Auto-colouring: Automatic selection of accessible + colour palettes for datasets where the consequence annotations are aligned with standard variant effect dictionaries (PAVE, SO, or MAF).

@@ -220,6 +224,9 @@ a Creative Commons Attribution 4.0 International License (CC BY Acknowledgements +

The results shown here are in whole or part based upon data + generated by the TCGA Research Network: + https://www.cancer.gov/tcga.

We thank the developers of the packages integral to ggoncoplot, especially David Gohel for ggiraph (Gohel diff --git a/paper/paper.bib b/paper/paper.bib index 7bc5f84..d9fe91d 100644 --- a/paper/paper.bib +++ b/paper/paper.bib @@ -56,11 +56,29 @@ @misc{pedersen:2024 url = {https://patchwork.data-imaginist.com} } - @Book{wickham:2016, - author = {Hadley Wickham}, - title = {ggplot2: Elegant Graphics for Data Analysis}, - publisher = {Springer-Verlag New York}, - year = {2016}, - isbn = {978-3-319-24277-4}, - url = {https://ggplot2.tidyverse.org}, - } \ No newline at end of file +@Book{wickham:2016, + author = {Hadley Wickham}, + title = {ggplot2: Elegant Graphics for Data Analysis}, + publisher = {Springer-Verlag New York}, + year = {2016}, + isbn = {978-3-319-24277-4}, + url = {https://ggplot2.tidyverse.org}, +} + + +@article{goldman:2020, + author = {Goldman, Mary J. and Craft, Brian and Hastie, Mim and Repe{\v c}ka, Kristupas and McDade, Fran and Kamath, Akhil and Banerjee, Ayan and Luo, Yunhai and Rogers, Dave and Brooks, Angela N. and Zhu, Jingchun and Haussler, David}, + date = {2020/06/01}, + date-added = {2024-08-05 12:48:11 +1000}, + date-modified = {2024-08-05 12:48:11 +1000}, + doi = {10.1038/s41587-020-0546-8}, + id = {Goldman2020}, + isbn = {1546-1696}, + journal = {Nature Biotechnology}, + number = {6}, + pages = {675--678}, + title = {Visualizing and interpreting cancer genomics data via the Xena platform}, + url = {https://doi.org/10.1038/s41587-020-0546-8}, + volume = {38}, + year = {2020}, + bdsk-url-1 = {https://doi.org/10.1038/s41587-020-0546-8}} diff --git a/paper/paper.md b/paper/paper.md index 8aee1e8..119e514 100644 --- a/paper/paper.md +++ b/paper/paper.md @@ -1,6 +1,5 @@ --- -title: 'ggoncoplot: an R package for visualising somatic mutation data from cancer - patient cohorts' +title: 'ggoncoplot: an R package for interactive visualisation of somatic mutation data from cancer patient cohorts' tags: - R - cancer @@ -30,12 +29,12 @@ affiliations: # Summary -The ggoncoplot R package generates interactive oncoplots to visualize mutational patterns across patient cancer cohorts (\autoref{fig:oncoplot}). Oncoplots, also called oncoprints, reveal patterns of gene co-mutation and include marginal plots that indicate co-occurrence of gene mutations with tumour and clinical features. It is useful to relate gene mutation patterns seen in an oncoplot to patterns in other plot types, including gene expression t-SNE plots or methylation UMAPs. The simplest and most intuitive approach to examining such relations is to link plots dynamically such that samples selected in an oncoplot can be highlighted in other plots. There are, however, no existing oncoplot-generating R packages that support dynamic data linkage between different plots. To address this gap and enable rapid exploration of a variety of data types we constructed the ggoncoplot package for the production of oncoplots that are easily integrated with custom visualisations and that support synchronised data-selections across plots (\autoref{fig:multimodal_selection}). ggoncoplot is available on GitHub at . +The ggoncoplot R package generates interactive oncoplots to visualize mutational patterns across patient cancer cohorts (\autoref{fig:oncoplot}). Oncoplots, also called oncoprints, reveal patterns of gene co-mutation and include marginal plots that indicate co-occurrence of gene mutations with tumour and clinical features. It is useful to relate gene mutation patterns seen in an oncoplot to patterns in other plot types, including gene expression t-SNE plots or methylation UMAPs. The simplest and most intuitive approach to examining such relations is to link plots dynamically such that samples selected in an oncoplot can be highlighted in other plots, and vice versa. There are, however, no existing oncoplot-generating R packages that support dynamic data linkage between different plots. To address this gap and enable rapid exploration of a variety of data types we constructed the ggoncoplot package for the production of oncoplots that are easily integrated with custom visualisations and that support synchronised data-selections across plots (\autoref{fig:multimodal_selection}). ggoncoplot is available on GitHub at . ![ggoncoplot output visualising mutational trends in the TCGA breast carcinoma cohort. Individual patient samples are plotted on the x-axis, hierarchically sorted so that samples with the most frequent gene mutations appear on the leftmost side. The plot indicates that PIK3CA is the most frequently mutated gene, followed by TP53. Marginal plots indicate the total number of mutations per sample (top), and the number of samples showing mutations in each gene, coloured by mutation type (right). A range of clinical features, including progesterone and estrogen receptor status are shown on the marginal plot at the bottom. A detailed description of the ggoncoplot sorting algorithm is available [here](https://selkamand.github.io/ggoncoplot/articles/sorting_algorithm.html). \label{fig:oncoplot}](oncoplot.pdf) -![Example of the ggoncoplot shown in Figure 1, where the oncoplot has been dynamically cross-linked to a gene expression t-SNE plot (top left) and a methylation UMAP (top right). Here, the lasso tool was used to select a cluster of gene expression data points (i.e., individual samples) in the t-SNE plot. Selected samples were automatically highlighted on the UMAP and oncoplot. This reveals that samples which cluster on the left of the t-SNE plot also cluster in the oncoplot, chiefly containing mutations in TP53 but wild type PIK3CA. The plots of progesterone, estrogen, HER2 status and triple negative classification show that the samples selected in the t-SNE are virtually all triple negative breast cancers. In contrast to the oncoplot, the methylation UMAP shows no strong clustering, in line with knowledge of methylation patterns in triple negative breast cancer. \label{fig:multimodal_selection}](multimodal_selection_with_lasso.png) +![Example of the ggoncoplot shown in Figure 1, where the oncoplot has been dynamically cross-linked to a gene expression t-SNE plot (top left) and a methylation UMAP (top right). Here, the lasso tool was used to select a cluster of gene expression data points (i.e., individual samples) in the t-SNE plot. Selected samples were automatically highlighted on the UMAP and oncoplot. This reveals that samples which cluster on the left of the t-SNE plot also cluster in the oncoplot, chiefly containing mutations in TP53 and wild type PIK3CA. The plots of progesterone, estrogen, HER2 status and triple negative classification show that the samples selected in the t-SNE are enriched for triple negative breast cancers. In contrast to the oncoplot, the methylation UMAP shows no strong clustering, consistent with knowledge of methylation patterns in triple negative breast cancer. Methylation, expression, and genome data were originally obtained from the UCSC Xena TCGA Pan-Cancer Atlas Hub [goldman:2020]. Expression and methylation plots were produced with [express](https://github.com/selkamand/express). \label{fig:multimodal_selection}](multimodal_selection_with_lasso.png) @@ -47,7 +46,7 @@ Oncoplots are highly effective for visualising mutation data in cancer cohorts b - **Support for tidy datasets**: Compatibility with tidy, tabular mutation-level formats (MAF files or relational databases), typical of cancer cohort datasets. This greatly improves the range of datasets that can be quickly and easily visualised in an oncoplot. -- **Auto-colouring**: Automatic selection of colour palettes for datasets where the consequence annotations are aligned with standard variant effect dictionaries (PAVE, SO, or MAF). +- **Auto-colouring**: Automatic selection of accessible colour palettes for datasets where the consequence annotations are aligned with standard variant effect dictionaries (PAVE, SO, or MAF). - **Versatility**: The ability to visualize entities other than gene mutations, such as noncoding features (e.g., promoter or enhancer mutations) and non-genomic entities (e.g., microbial presence in microbiome datasets). @@ -59,6 +58,8 @@ We developed ggoncoplot as the first R package to address all these challenges t # Acknowledgements +The results shown here are in whole or part based upon data generated by the TCGA Research Network: https://www.cancer.gov/tcga. + We thank the developers of the packages integral to ggoncoplot, especially David Gohel for ggiraph [@gohel:2024], which enables its interactivity, and Thomas Lin Pedersen for patchwork [@pedersen:2024] and ggplot2 maintenance. We also acknowledge Hadley Wickham and all contributors to ggplot2 [@wickham:2016]. 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+++++++++++++++++++++++++++++++++++------- paper/paper.docx | Bin 644088 -> 644315 bytes paper/paper.md | 4 ++-- paper/paper.pdf | Bin 727028 -> 727817 bytes 4 files changed, 39 insertions(+), 9 deletions(-) diff --git a/paper/jats/paper.jats b/paper/jats/paper.jats index 5597371..3edb534 100644 --- a/paper/jats/paper.jats +++ b/paper/jats/paper.jats @@ -145,11 +145,10 @@ a Creative Commons Attribution 4.0 International License (CC BY selected in the t-SNE are enriched for triple negative breast cancers. In contrast to the oncoplot, the methylation UMAP shows no strong clustering, consistent with knowledge of methylation patterns - in triple negative breast cancer. Methylation, expression, and - genome data were originally obtained from the UCSC Xena TCGA - Pan-Cancer Atlas Hub [goldman:2020]. Expression and methylation - plots were produced with - express. + in triple negative breast cancer. Expression and methylation plots + were produced using the + express + package.

@@ -225,8 +224,11 @@ a Creative Commons Attribution 4.0 International License (CC BY Acknowledgements

The results shown here are in whole or part based upon data - generated by the TCGA Research Network: - https://www.cancer.gov/tcga.

+ generated by the TCGA Research Network: https://www.cancer.gov/tcga. + Methylation, expression, and genome datasets were obtained from the + Xena TCGA Pan-Cancer Atlas Hub + (Goldman + et al., 2020).

We thank the developers of the packages integral to ggoncoplot, especially David Gohel for ggiraph (Gohel @@ -336,6 +338,34 @@ a Creative Commons Attribution 4.0 International License (CC BY https://ggplot2.tidyverse.org + + + + GoldmanMary J. + CraftBrian + HastieMim + RepečkaKristupas + McDadeFran + KamathAkhil + BanerjeeAyan + LuoYunhai + RogersDave + BrooksAngela N. + ZhuJingchun + HausslerDavid + + Visualizing and interpreting cancer genomics data via the xena platform + Nature Biotechnology + 2020 + 38 + 6 + 1546-1696 + https://doi.org/10.1038/s41587-020-0546-8 + 10.1038/s41587-020-0546-8 + 675 + 678 + + diff --git a/paper/paper.docx b/paper/paper.docx index feda7871d9389b27d9f7780a7d54413a49d0d92d..75b9f1c20a0a73bab453ea4e6e33fb2a5a23ae14 100644 GIT binary patch delta 5827 zcmZ8l1yodR^Iw)+Sh~9#mPQ&RC0ywi0ck;Sr3IFhSW1bd6eLBKZjcmd=|<`9k`m#M zyx;%4pWi)a&Yk<4ndi>)Ox$zlynu7Ikdstf6CHyL0K~xo*z#hU^abl}6qJ+Zq4BG) 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