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I have a script that pulls the cell segmentation mask from a spatialdata io object from Xenium and exports it as a tiff for our visualization software. The data are taken from this publicly available breast dataset:
I have a script that pulls the cell segmentation mask from a spatialdata io object from Xenium and exports it as a tiff for our visualization software. The data are taken from this publicly available breast dataset:
However, upon exporting the mask to tiff format, the coordinates in the y-axis are flipped:
If I flip the mask array among the y-axis prior to export, the mask now aligns with the expression results:
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