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example.config
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example.config
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// Input/output Parameters
params.input_path = "input"
params.output_path = "output"
// Workflow control parameters
params.fill_gaps = true // Set to false to skip MindaGap Gap Filling
params.deduplicate = true // Set to false to skip MindaGap transcript deduplication
params.do_zip = true // Set to false to skip making ImageJ ROIs (faster)
params.segmentation_tool = "mesmer" // Use Mesmer (mesmer) or Cellpose (cellpose) for segmentation
// MindaGap transcript deduplication parameters
params.tile_size = 2144 // Tile size (distance between gridlines)
params.window_size = 30 // Window arround gridlines to search for duplicates
params.max_freq = 400 // Maximum transcript count to calculate X/Y shifts (better to discard very common genes)
params.min_mode = 10 // Minumum occurances of ~XYZ_shift to consider it valid
// Cellpose Segmentation Parameters
params.model_name = "cyto"
params.probability_threshold = 1
params.cell_diameter = 70
// Mesmer Segmentation Parameters
params.maxima_threshold = 0.075 // Decrease for over segmentation. Default(0.075)
params.maxima_smooth = 0 // Smoothing radius for maxima. Default(0)
params.interior_threshold = 0.2 // Decrease to identify more cells. Default(0.2)
params.interior_smooth = 2 // Smoothing radius for cell detection. Default(2)
params.small_objects_threshold = 15 // Minimum object size. Default(15)
params.fill_holes_threshold = 15 // Max Size for hole filling. Default(15)
params.radius = 2 // Undocuented in Mesmer. Default(2)