diff --git a/workflow/scripts/AncestryInformativeMarkers.py b/workflow/scripts/AncestryInformativeMarkers.py
index 5686914..e7763eb 100644
--- a/workflow/scripts/AncestryInformativeMarkers.py
+++ b/workflow/scripts/AncestryInformativeMarkers.py
@@ -38,7 +38,7 @@
 
     # read in and filter data
     path = f"results/variantAnalysis/vcfs/{dataset}.{contig}.vcf.gz"
-    vcf, geno, acsubpops, pos, depth, snpeff, subpops, pops =  rnaseqpop.readAndFilterVcf(path=path,
+    vcf, geno, acsubpops, pos, alts, depth, snpeff, subpops, pops =  rnaseqpop.readAndFilterVcf(path=path,
                                                                contig=contig,
                                                                samples=metadata,
                                                                ploidy=ploidy,