diff --git a/README.md b/README.md index 31512bc0..c7b92970 100644 --- a/README.md +++ b/README.md @@ -20,8 +20,8 @@ It takes a samplesheet of BAM/CRAM/FASTQ/FASTA files as input, calculates genome 4. Run BUSCO ([`busco`](https://busco.ezlab.org/)) 5. Extract BUSCO genes ([`blobtoolkit/extractbuscos`](https://github.com/blobtoolkit/blobtoolkit)) 6. Run Diamond BLASTp against extracted BUSCO genes ([`diamond/blastp`](https://github.com/bbuchfink/diamond)) -7. Run BLASTn against extracted BUSCO genes ([`blast/blastn`](https://www.ncbi.nlm.nih.gov/books/NBK131777/)) -8. Run BLASTx against extracted BUSCO genes ([`blast/blastx`](https://www.ncbi.nlm.nih.gov/books/NBK131777/)) +7. Run BLASTx against sequences with no hit ([`blast/blastn`](https://www.ncbi.nlm.nih.gov/books/NBK131777/)) +8. Run BLASTn against sequences still with not hit ([`blast/blastx`](https://www.ncbi.nlm.nih.gov/books/NBK131777/)) 9. Count BUSCO genes ([`blobtoolkit/countbuscos`](https://github.com/blobtoolkit/blobtoolkit)) 10. Generate combined sequence stats across various window sizes ([`blobtoolkit/windowstats`](https://github.com/blobtoolkit/blobtoolkit)) 11. Imports analysis results into a BlobDir dataset ([`blobtoolkit/blobdir`](https://github.com/blobtoolkit/blobtoolkit))