From d6e6e724402349241355005d74153ab986a7ee68 Mon Sep 17 00:00:00 2001 From: matthewfallan <31744230+matthewfallan@users.noreply.github.com> Date: Wed, 15 Nov 2023 13:03:14 -0500 Subject: [PATCH 1/9] Fix bug while finding unique reads wherein positions with no mutations would get arrays of floats instead of integers --- src/seismicrna/clust/uniq.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/src/seismicrna/clust/uniq.py b/src/seismicrna/clust/uniq.py index 8734fad8..4a3b86dd 100644 --- a/src/seismicrna/clust/uniq.py +++ b/src/seismicrna/clust/uniq.py @@ -129,7 +129,7 @@ def uniq_reads_to_mutations(uniq_reads: Iterable[tuple], for uniq_read_num, read_muts_pos in enumerate(uniq_reads): for pos in read_muts_pos: mutations[pos].append(uniq_read_num) - return [np.array(mutations[pos]) for pos in pos_nums] + return [np.array(mutations[pos], dtype=int) for pos in pos_nums] def count_uniq_reads(uniq_read_nums: Iterable[list]): From 34dee81522eefabd6ffc109fad978da19d15822b Mon Sep 17 00:00:00 2001 From: matthewfallan <31744230+matthewfallan@users.noreply.github.com> Date: Thu, 16 Nov 2023 13:26:55 -0500 Subject: [PATCH 2/9] Update relate batch docs --- docs/.doctrees/api/seismicrna.clust.doctree | Bin 276476 -> 284022 bytes .../api/seismicrna.core.batch.doctree | Bin 235598 -> 235376 bytes docs/.doctrees/api/seismicrna.core.doctree | Bin 662158 -> 662138 bytes .../.doctrees/api/seismicrna.core.seq.doctree | Bin 248287 -> 246817 bytes .../api/seismicrna.core.seq.tests.doctree | Bin 267304 -> 266221 bytes docs/.doctrees/api/seismicrna.table.doctree | Bin 340108 -> 352267 bytes .../.doctrees/api/seismicrna.workflow.doctree | Bin 206952 -> 212997 bytes docs/.doctrees/cli.doctree | Bin 354054 -> 362746 bytes docs/.doctrees/data/index.doctree | Bin 2717 -> 2723 bytes .../{relate.doctree => relate/codes.doctree} | Bin 68332 -> 68345 bytes docs/.doctrees/data/relate/index.doctree | Bin 0 -> 2793 bytes docs/.doctrees/data/relate/relate.doctree | Bin 0 -> 44257 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src/userdocs/formats/data/fastq.rst | 4 +- src/userdocs/manuals/workflow.rst | 59 ++- src/userdocs/writeme.rst | 8 +- 61 files changed, 1509 insertions(+), 471 deletions(-) rename docs/.doctrees/data/{relate.doctree => relate/codes.doctree} (98%) create mode 100644 docs/.doctrees/data/relate/index.doctree create mode 100644 docs/.doctrees/data/relate/relate.doctree create mode 100644 docs/.doctrees/manuals/parallel.doctree rename docs/_sources/data/{relate.rst.txt => relate/codes.rst.txt} (100%) create mode 100644 docs/_sources/data/relate/index.rst.txt create mode 100644 docs/_sources/data/relate/relate.rst.txt create mode 100644 docs/_sources/manuals/parallel.rst.txt rename docs/data/{relate.html => relate/codes.html} (84%) create mode 100644 docs/data/relate/index.html create mode 100644 docs/data/relate/relate.html create mode 100644 docs/manuals/parallel.html delete mode 100644 src/userdocs/data/relate.rst create mode 100644 src/userdocs/data/relate/index.rst create mode 100644 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z1{33|$+m3v?1?1`C5gq^lf&6%4K;NXN-{Ew6;kq(OLJ56N)k&l^Yau^^HPcx$}>wc z6w*sGQ&Mv>^HPhs((;QG%8MrNV;AShV2_=WA=a}FXq@ETDz3XZ zf%yg# - + @@ -89,7 +89,7 @@

Algorithms - + diff --git a/docs/api/seismicrna.clust.html b/docs/api/seismicrna.clust.html index f62e6e54..4ee889de 100644 --- a/docs/api/seismicrna.clust.html +++ b/docs/api/seismicrna.clust.html @@ -447,8 +447,23 @@

Cluster Comparison Module
-run()
+run(props_seed: Optional[int] = None, resps_seed: Optional[int] = None)

Run the EM clustering algorithm.

+
+
Parameters
+
    +
  • props_seed (int | None = None) – Random number generator seed for cluster proportions

  • +
  • resps_seed (int | None = None) – Random number generator seed for read responsibilities

  • +
+
+
Returns
+

This instance, in order to permit statements such as +return [em.run() for em in em_clusterings]

+
+
Return type
+

EmClustering

+
+
@@ -582,7 +597,7 @@

Cluster – Names Module
-class seismicrna.clust.uniq.UniqReads(sample: str, ref: str, section: seismicrna.core.seq.section.Section, min_mut_gap: int, muts_per_pos: list[numpy.ndarray], batch_to_uniq: list[pandas.core.series.Series], counts_per_uniq: numpy.ndarray)
+class seismicrna.clust.uniq.UniqReads(sample: str, section: seismicrna.core.seq.section.Section, min_mut_gap: int, muts_per_pos: list[numpy.ndarray], batch_to_uniq: list[pandas.core.series.Series], counts_per_uniq: numpy.ndarray)

Bases: object

Collection of bit vectors of unique reads.

@@ -631,6 +646,12 @@

Cluster – Names Moduleproperty pos_nums

+
+
+property ref
+

Reference name.

+
+
diff --git a/docs/api/seismicrna.core.batch.html b/docs/api/seismicrna.core.batch.html index 6d622331..74e98cfa 100644 --- a/docs/api/seismicrna.core.batch.html +++ b/docs/api/seismicrna.core.batch.html @@ -133,15 +133,13 @@
seismicrna.core.batch.count.get_count_per_pos(pattern: seismicrna.core.rel.pattern.RelPattern, cover_per_pos: pandas.core.series.Series | pandas.core.frame.DataFrame, rels_per_pos: dict[int, pandas.core.series.Series | pandas.core.frame.DataFrame])
-

Count the reads that fit a relationship pattern at each -position in a section.

+

Count the reads that fit a pattern at each position.

seismicrna.core.batch.count.get_count_per_read(pattern: seismicrna.core.rel.pattern.RelPattern, cover_per_read: pandas.core.frame.DataFrame, rels_per_read: dict[int, pandas.core.frame.DataFrame], read_weights: Optional[pandas.core.frame.DataFrame] = None)
-

Count the positions in a section that fit a relationship -pattern in each read.

+

Count the positions that fit a pattern in each read.

@@ -169,22 +167,19 @@
seismicrna.core.batch.count.get_reads_per_pos(pattern: seismicrna.core.rel.pattern.RelPattern, mutations: dict[int, dict[int, numpy.ndarray]], pos_index: pandas.core.indexes.base.Index)
-

For each position, find all reads matching a relationship -pattern.

+

For each position, find all reads matching a pattern.

seismicrna.core.batch.count.get_rels_per_pos(mutations: dict[int, dict[int, numpy.ndarray]], num_reads: int | pandas.core.series.Series, cover_per_pos: pandas.core.series.Series | pandas.core.frame.DataFrame, read_indexes: Optional[numpy.ndarray] = None, read_weights: Optional[pandas.core.frame.DataFrame] = None)
-

For each relationship, the number of reads at each position -with that relationship.

+

For each relationship, the number of reads at each position.

seismicrna.core.batch.count.get_rels_per_read(mutations: dict[int, dict[int, numpy.ndarray]], pos_index: pandas.core.indexes.base.Index, cover_per_read: pandas.core.frame.DataFrame, read_indexes: numpy.ndarray)
-

For each relationship, the number of positions in each read -with that relationship.

+

For each relationship, the number of positions in each read.

diff --git a/docs/api/seismicrna.core.html b/docs/api/seismicrna.core.html index ba2dd66d..106bace5 100644 --- a/docs/api/seismicrna.core.html +++ b/docs/api/seismicrna.core.html @@ -1450,7 +1450,7 @@

Random Core Module
-save(top: pathlib.Path, overwrite: bool = False)
+save(top: pathlib.Path, force: bool = False)

Save the report to a JSON file.

@@ -1760,17 +1760,17 @@

Random Core Module
-seismicrna.core.version.compatible_versions(version1: str, version2: str = '0.9.3')
+seismicrna.core.version.compatible_versions(version1: str, version2: str = '0.9.4')

-seismicrna.core.version.format_version(major: int = 0, minor: int = 9, patch: int = 3)
+seismicrna.core.version.format_version(major: int = 0, minor: int = 9, patch: int = 4)
-seismicrna.core.version.parse_version(version: str = '0.9.3')
+seismicrna.core.version.parse_version(version: str = '0.9.4')
diff --git a/docs/api/seismicrna.core.seq.html b/docs/api/seismicrna.core.seq.html index cef9aab8..4211212c 100644 --- a/docs/api/seismicrna.core.seq.html +++ b/docs/api/seismicrna.core.seq.html @@ -345,12 +345,6 @@

Subpackages -
-property unmasked_zero
-

Unmasked 0-indexed positions as integers.

-

-
diff --git a/docs/api/seismicrna.core.seq.tests.html b/docs/api/seismicrna.core.seq.tests.html index 8732f5c0..27aca244 100644 --- a/docs/api/seismicrna.core.seq.tests.html +++ b/docs/api/seismicrna.core.seq.tests.html @@ -725,11 +725,6 @@ test_unmasked_int()
-
-
-test_unmasked_zero()
-
-

diff --git a/docs/api/seismicrna.table.html b/docs/api/seismicrna.table.html index 48797103..787d05b4 100644 --- a/docs/api/seismicrna.table.html +++ b/docs/api/seismicrna.table.html @@ -842,13 +842,17 @@
-seismicrna.table.main.run(input_path: tuple[str, ...], max_procs: int = 16, parallel: bool = True, **kwargs)
+seismicrna.table.main.run(input_path: tuple[str, ...], *, table_pos: bool = True, table_read: bool = True, table_clust: bool = True, force: bool = False, max_procs: int = 16, parallel: bool = True)

Run the table module.

Parameters
    -
  • max_procs (int) – Maximum number of simultaneous processes [positional or keyword, default: 16]

  • -
  • parallel (bool) – Run tasks in parallel [positional or keyword, default: True]

  • +
  • table_pos (bool) – Tabulate per position [keyword-only, default: True]

  • +
  • table_read (bool) – Tabulate per read [keyword-only, default: True]

  • +
  • table_clust (bool) – Tabulate per cluster [keyword-only, default: True]

  • +
  • force (bool) – Force all tasks to run, even those whose output files already exist [keyword-only, default: False]

  • +
  • max_procs (int) – Maximum number of simultaneous processes [keyword-only, default: 16]

  • +
  • parallel (bool) – Run tasks in parallel [keyword-only, default: True]

@@ -957,7 +961,7 @@
-seismicrna.table.write.get_tabulator_writers(tabulator: seismicrna.table.calc.AvgTabulator | seismicrna.table.calc.ClustTabulator)
+seismicrna.table.write.get_tabulator_writers(tabulator: seismicrna.table.calc.AvgTabulator | seismicrna.table.calc.ClustTabulator, *, table_pos: bool = True, table_read: bool = True, table_clust: bool = True)
@@ -968,7 +972,7 @@
-seismicrna.table.write.write(report_file: pathlib.Path, force: bool)
+seismicrna.table.write.write(report_file: pathlib.Path, *, force: bool, **kwargs)

Helper function to write a table from a report file.

diff --git a/docs/api/seismicrna.workflow.html b/docs/api/seismicrna.workflow.html index b20682b1..552e3822 100644 --- a/docs/api/seismicrna.workflow.html +++ b/docs/api/seismicrna.workflow.html @@ -114,7 +114,7 @@

Whole Pipeline Main Module

-seismicrna.workflow.main.run(*, input_path: tuple[str, ...], out_dir: str = './out', temp_dir: str = './temp', keep_temp: bool = False, brotli_level: int = 10, force: bool = False, max_procs: int = 16, parallel: bool = True, fasta: str, fastqz: tuple[str, ...] = (), fastqy: tuple[str, ...] = (), fastqx: tuple[str, ...] = (), phred_enc: int = 33, demulti_overwrite: bool = False, demult_on: bool = False, parallel_demultiplexing: bool = False, clipped: int = 0, mismatch_tolerence: int = 0, index_tolerance: int = 0, barcode_start: int = 0, barcode_length: int = 0, dmfastqz: tuple[str, ...] = (), dmfastqy: tuple[str, ...] = (), dmfastqx: tuple[str, ...] = (), fastqc: bool = True, qc_extract: bool = False, cut: bool = True, cut_q1: int = 25, cut_q2: int = 25, cut_g1: tuple[str, ...] = ('GCTCTTCCGATCT',), cut_a1: tuple[str, ...] = ('AGATCGGAAGAGC',), cut_g2: tuple[str, ...] = ('GCTCTTCCGATCT',), cut_a2: tuple[str, ...] = ('AGATCGGAAGAGC',), cut_o: int = 6, cut_e: float = 0.1, cut_indels: bool = True, cut_nextseq: bool = False, cut_discard_trimmed: bool = False, cut_discard_untrimmed: bool = False, cut_m: int = 20, bt2_local: bool = True, bt2_discordant: bool = False, bt2_mixed: bool = False, bt2_dovetail: bool = False, bt2_contain: bool = True, bt2_score_min_e2e: str = 'L,-1,-0.5', bt2_score_min_loc: str = 'L,1,0.5', bt2_i: int = 0, bt2_x: int = 600, bt2_gbar: int = 4, bt2_l: int = 20, bt2_s: str = 'L,1,0.1', bt2_d: int = 4, bt2_r: int = 2, bt2_dpad: int = 2, bt2_orient: str = 'fr', bt2_un: bool = True, min_mapq: int = 25, cram: bool = True, min_phred: int = 25, min_reads: int = 1000, ambrel: bool = True, batch_size: float = 64.0, coords: tuple[tuple[str, int, int], ...] = (), primers: tuple[tuple[str, seismicrna.core.seq.xna.DNA, seismicrna.core.seq.xna.DNA], ...] = (), primer_gap: int = 2, sections_file: str = '', count_del: bool = False, count_ins: bool = False, discount_mut: tuple[str, ...] = (), exclude_polya: int = 5, exclude_gu: bool = True, exclude_pos: tuple[tuple[str, int], ...] = (), min_finfo_read: float = 0.95, max_fmut_read: int = 0.1, min_mut_gap: int = 3, min_ninfo_pos: int = 1000, max_fmut_pos: float = 0.5, max_clusters: int = 0, min_nmut_read: int = 0, em_runs: int = 6, min_em_iter: int = 10, max_em_iter: int = 300, em_thresh: float = 0.01, fold: bool = False, quantile: float = 0.0)
+seismicrna.workflow.main.run(*, input_path: tuple[str, ...], out_dir: str = './out', temp_dir: str = './temp', keep_temp: bool = False, brotli_level: int = 10, force: bool = False, max_procs: int = 16, parallel: bool = True, fasta: str, fastqz: tuple[str, ...] = (), fastqy: tuple[str, ...] = (), fastqx: tuple[str, ...] = (), phred_enc: int = 33, demulti_overwrite: bool = False, demult_on: bool = False, parallel_demultiplexing: bool = False, clipped: int = 0, mismatch_tolerence: int = 0, index_tolerance: int = 0, barcode_start: int = 0, barcode_length: int = 0, dmfastqz: tuple[str, ...] = (), dmfastqy: tuple[str, ...] = (), dmfastqx: tuple[str, ...] = (), fastqc: bool = True, qc_extract: bool = False, cut: bool = True, cut_q1: int = 25, cut_q2: int = 25, cut_g1: tuple[str, ...] = ('GCTCTTCCGATCT',), cut_a1: tuple[str, ...] = ('AGATCGGAAGAGC',), cut_g2: tuple[str, ...] = ('GCTCTTCCGATCT',), cut_a2: tuple[str, ...] = ('AGATCGGAAGAGC',), cut_o: int = 6, cut_e: float = 0.1, cut_indels: bool = True, cut_nextseq: bool = False, cut_discard_trimmed: bool = False, cut_discard_untrimmed: bool = False, cut_m: int = 20, bt2_local: bool = True, bt2_discordant: bool = False, bt2_mixed: bool = False, bt2_dovetail: bool = False, bt2_contain: bool = True, bt2_score_min_e2e: str = 'L,-1,-0.5', bt2_score_min_loc: str = 'L,1,0.5', bt2_i: int = 0, bt2_x: int = 600, bt2_gbar: int = 4, bt2_l: int = 20, bt2_s: str = 'L,1,0.1', bt2_d: int = 4, bt2_r: int = 2, bt2_dpad: int = 2, bt2_orient: str = 'fr', bt2_un: bool = True, min_mapq: int = 25, cram: bool = True, min_phred: int = 25, min_reads: int = 1000, ambrel: bool = True, batch_size: float = 64.0, coords: tuple[tuple[str, int, int], ...] = (), primers: tuple[tuple[str, seismicrna.core.seq.xna.DNA, seismicrna.core.seq.xna.DNA], ...] = (), primer_gap: int = 2, sections_file: str = '', count_del: bool = False, count_ins: bool = False, discount_mut: tuple[str, ...] = (), exclude_polya: int = 5, exclude_gu: bool = True, exclude_pos: tuple[tuple[str, int], ...] = (), min_finfo_read: float = 0.95, max_fmut_read: int = 0.1, min_mut_gap: int = 3, min_ninfo_pos: int = 1000, max_fmut_pos: float = 0.5, max_clusters: int = 0, min_nmut_read: int = 0, em_runs: int = 6, min_em_iter: int = 10, max_em_iter: int = 300, em_thresh: float = 0.01, table_pos: bool = True, table_read: bool = True, table_clust: bool = True, fold: bool = False, quantile: float = 0.0)

Run entire pipeline.

Parameters
@@ -201,6 +201,9 @@

Whole Pipeline Main Moduleint) – Minimum iterations per clustering run [keyword-only, default: 10]

  • max_em_iter (int) – Maximum iterations per clustering run [keyword-only, default: 300]

  • em_thresh (float) – Maximum change in log likelihood for convergence [keyword-only, default: 0.01]

  • +
  • table_pos (bool) – Tabulate per position [keyword-only, default: True]

  • +
  • table_read (bool) – Tabulate per read [keyword-only, default: True]

  • +
  • table_clust (bool) – Tabulate per cluster [keyword-only, default: True]

  • fold (bool) – Predict the secondary structure using the RNAstructure Fold program [keyword-only, default: False]

  • quantile (float) – Quantile of mutation rates for normalization; must be in [0, 1] [keyword-only, default: 0.0]

  • diff --git a/docs/cli.html b/docs/cli.html index 31562e89..e42b3066 100644 --- a/docs/cli.html +++ b/docs/cli.html @@ -625,6 +625,24 @@

    Command Line Interface

    Maximum iterations per clustering run

    +
    +
    +--table-pos, --no-table-pos
    +

    Tabulate per position

    +
    + +
    +
    +--table-read, --no-table-read
    +

    Tabulate per read

    +
    + +
    +
    +--table-clust, --no-table-clust
    +

    Tabulate per cluster

    +
    +
    --fold, --no-fold
    @@ -1362,15 +1380,21 @@

    Command Line Interface

    Options

    -
    ---max-procs <max_procs>
    -

    Maximum number of simultaneous processes

    +
    +--table-pos, --no-table-pos
    +

    Tabulate per position

    -
    ---parallel, --serial
    -

    Run tasks in parallel

    +
    +--table-read, --no-table-read
    +

    Tabulate per read

    +
    + +
    +
    +--table-clust, --no-table-clust
    +

    Tabulate per cluster

    @@ -1379,6 +1403,18 @@

    Command Line Interface

    Force all tasks to run, even those whose output files already exist

    +
    +
    +--max-procs <max_procs>
    +

    Maximum number of simultaneous processes

    +
    + +
    +
    +--parallel, --serial
    +

    Run tasks in parallel

    +
    +

    Arguments

    diff --git a/docs/data/index.html b/docs/data/index.html index 4b124cd3..9e84a59c 100644 --- a/docs/data/index.html +++ b/docs/data/index.html @@ -19,7 +19,7 @@ - + @@ -52,7 +52,7 @@
  • seismicrna package
  • File Formats
  • Data Structures
  • Algorithms
  • @@ -88,12 +88,9 @@

    Data Structures


    diff --git a/docs/data/relate.html b/docs/data/relate/codes.html similarity index 84% rename from docs/data/relate.html rename to docs/data/relate/codes.html index c2bac5e0..b0cd68ff 100644 --- a/docs/data/relate.html +++ b/docs/data/relate/codes.html @@ -5,22 +5,22 @@ Relation Vectors — seismic-rna documentation - - - + + + - - - - - - - - - + + + + + + + + + @@ -31,12 +31,12 @@ - + seismic-rna - +
    -
    + @@ -44,35 +44,31 @@
    @@ -81,18 +77,19 @@

    @@ -120,7 +117,7 @@

    Relationships between reads and references +../../_images/relationships.png

    Encoding primary relationships

    @@ -519,8 +516,8 @@

    Irreconcilable relationships - - + +
    diff --git a/docs/data/relate/index.html b/docs/data/relate/index.html new file mode 100644 index 00000000..7da9794d --- /dev/null +++ b/docs/data/relate/index.html @@ -0,0 +1,144 @@ + + + + + + + Relate Data Structures — seismic-rna documentation + + + + + + + + + + + + + + + + + + + + + + \ No newline at end of file diff --git a/docs/data/relate/relate.html b/docs/data/relate/relate.html new file mode 100644 index 00000000..a3778ae0 --- /dev/null +++ b/docs/data/relate/relate.html @@ -0,0 +1,382 @@ + + + + + + + Relate Batch — seismic-rna documentation + + + + + + + + + + + + + + + + + +
    + + +
    + +
    +
    +
    + +
    +
    +
    +
    + +
    +

    Relate Batch

    +

    Each batch of relation vectors is a RelateBatchIO object.

    +
    +

    Relate batch: Specifications

    +

    The following attributes encode the relationships between each read and +each position in the reference sequence:

    + +++++ + + + + + + + + + + + + + + + + + + + + + + + + + + + + +

    Attribute

    Data Type

    Description

    end5s

    numpy.ndarray[int]

    array of the first position of the most upstream mate in each read

    mid5s

    numpy.ndarray[int]

    array of the first position of the most downstream mate in each read

    mid3s

    numpy.ndarray[int]

    array of the last position of the most upstream mate in each read

    end3s

    numpy.ndarray[int]

    array of the last position of the most downstream mate in each read

    muts

    dict[int, dict[int, numpy.ndarray[int]]]

    array of the reads with each type of mutation at each position

    +
    +

    Note

    +

    The positions of the first and last bases in the reference sequence +are defined to be 1 and the length of the sequence, respectively.

    +
    +
    +

    Relate batch: Specifications of read numbers

    +

    Each read, or pair of paired-end reads, is labeled with a non-negative +integer: 0 for the first read in each batch, and incrementing by 1 for +each subsequent read. +Within one batch, all read numbers are unique. +However, two different batches can have reads that share numbers.

    +
    +
    +

    Relate batch: Specifications of 5’ and 3’ end positions

    +
      +
    • end5s, mid5s, mid3s, and end3s are all 1-dimensional +numpy.ndarray objects.

    • +
    • For any relate batch, end5s, mid5s, mid3s, and end3s +all have the same length (which may be any integer ≥ 0).

    • +
    • A read with index i corresponds to the ith values of +end5s, mid5s, mid3s, and end3s; denoted (respectively) +end5s[i], mid5s[i], mid3s[i], and end3s[i].

    • +
    • For every read i:

      +
        +
      • 1 ≤ end5s[i]end3s[i] ≤ length of reference sequence

      • +
      • If paired-end and there is a gap of ≥ 1 nt between mates 1 and 2:

        +
          +
        • end5s[i]mid3s[i] < mid5s[i]end3s[i]

        • +
        +
      • +
      • Otherwise:

        +
          +
        • end5s[i] = mid5s[i]mid3s[i] = end3s[i]

        • +
        +
      • +
      +
    • +
    +
    +
    +

    Relate batch: Specifications of mutations

    +

    muts is a dict wherein

    +
      +
    • each key is a position in the reference sequence (int)

    • +
    • each value is a dict wherein

      +
        +
      • each key is a type of mutation (int, see Relation Vectors for more +information)

      • +
      • each value is an array of the numbers of the reads that have the +given type of mutation at the given position (numpy.ndarray)

      • +
      +
    • +
    +
    +
    +
    +

    Relate batch: Example

    +

    For example, suppose that the reference sequence is TCAGAACC and a +batch contains five paired-end reads, numbered 0 to 4:

    + +++++++ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +

    Read

    5’ End

    5’ Mid

    3’ Mid

    3’ End

    0

    2

    4

    6

    8

    1

    1

    1

    6

    6

    2

    2

    3

    5

    7

    3

    1

    5

    7

    8

    4

    3

    3

    7

    7

    +

    The positions, reads, and relationships can be shown explicitly as a +matrix (see Relation Vectors for an explanation of the relationships):

    + +++++++++++ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +

    Read

    1

    2

    3

    4

    5

    6

    7

    8

    0

    255

    1

    1

    1

    255

    1

    16

    1

    1

    1

    1

    128

    1

    128

    1

    255

    255

    2

    255

    1

    1

    255

    1

    1

    1

    255

    3

    1

    64

    1

    1

    128

    255

    1

    1

    4

    255

    255

    1

    1

    3

    3

    1

    255

    +

    In a relate batch, they would be encoded as follows:

    +
      +
    • end5s: [2, 1, 2, 1, 3]

    • +
    • mid5s: [4, 1, 3, 5, 3]

    • +
    • mid3s: [6, 6, 5, 7, 7]

    • +
    • end3s: [8, 6, 7, 8, 7]

    • +
    • muts:

      +
      {1: {},
      + 2: {64: [3]},
      + 3: {128: [1]},
      + 4: {},
      + 5: {3: [4], 128: [1, 3]},
      + 6: {3: [4]},
      + 7: {16: [0]},
      + 8: {}
      +
      +
      +

      Note that the numbers are shown here for visual simplicity as list +objects, but would really by numpy.ndarray objects.

      +
    • +
    +
    +
    + + +
    +
    + +
    +
    +
    +
    + + + + \ No newline at end of file diff --git a/docs/formats/data/fastq.html b/docs/formats/data/fastq.html index cb77f3db..bfe8ae0b 100644 --- a/docs/formats/data/fastq.html +++ b/docs/formats/data/fastq.html @@ -124,9 +124,9 @@

    FASTQ DNA alphabetPhred+N) and then converting the number to the character diff --git a/docs/genindex.html b/docs/genindex.html index c9bcbf3f..a66296dd 100644 --- a/docs/genindex.html +++ b/docs/genindex.html @@ -853,8 +853,6 @@

    Symbols

  • seismic-demult command line option
  • - - + + +
  • + --no-table-clust + +
  • +
  • + --no-table-pos + +
  • +
  • + --no-table-read + +
  • @@ -1299,6 +1326,33 @@

    Symbols

  • seismic-relate command line option
  • seismic-table command line option +
  • + +
  • + --table-clust + +
  • +
  • + --table-pos + +
  • +
  • + --table-read + +
  • @@ -4029,9 +4083,11 @@

    R

  • RED (seismicrna.core.logs.AnsiCode attribute)
  • -
  • ref (seismicrna.core.data.Dataset property) +
  • ref (seismicrna.clust.uniq.UniqReads property)
  • test_pos_index() (seismicrna.core.seq.tests.section_test.TestConstants method) -
  • -
  • test_pos_name() (seismicrna.core.seq.tests.section_test.TestConstants method)
  • diff --git a/docs/index.html b/docs/index.html index fa04ea59..95c98c6b 100644 --- a/docs/index.html +++ b/docs/index.html @@ -106,6 +106,7 @@

    SEISMIC-RNA User GuideRunning the Workflow
  • Cleaning FASTA Files
  • Listing Input Files
  • +
  • Parallelizing Tasks
  • Logging Messages
  • @@ -134,7 +135,7 @@

    SEISMIC-RNA User Guide
  • Data Structures
  • Algorithms
  • diff --git a/docs/manuals/faclean.html b/docs/manuals/faclean.html index 8439466b..6b1f3bc9 100644 --- a/docs/manuals/faclean.html +++ b/docs/manuals/faclean.html @@ -66,6 +66,7 @@
  • Listing Input Files
  • +
  • Parallelizing Tasks
  • Logging Messages
  • diff --git a/docs/manuals/index.html b/docs/manuals/index.html index fdb47911..bdb2ac1c 100644 --- a/docs/manuals/index.html +++ b/docs/manuals/index.html @@ -51,6 +51,7 @@
  • Running the Workflow
  • Cleaning FASTA Files
  • Listing Input Files
  • +
  • Parallelizing Tasks
  • Logging Messages
  • @@ -113,6 +114,10 @@

    ManualsGeneral methods to list input files as positional arguments +
  • Parallelizing Tasks +
  • Logging Messages
  • +
  • Parallelizing Tasks
  • Logging Messages
  • @@ -155,7 +156,7 @@

    General methods to list input files as positional arguments - +
    diff --git a/docs/manuals/logging.html b/docs/manuals/logging.html index e2d6b27f..76508a60 100644 --- a/docs/manuals/logging.html +++ b/docs/manuals/logging.html @@ -20,7 +20,7 @@ - + @@ -51,6 +51,7 @@
  • Running the Workflow
  • Cleaning FASTA Files
  • Listing Input Files
  • +
  • Parallelizing Tasks
  • Logging Messages
  • +
    +
    How to filter by number of aligned reads
    +

    In general, alignment maps containing very few reads are not useful for +mutational profiling, due to their inherently low coverage per position. +Worse, if aligning to a very large number of references (e.g. an entire +transcriptome), most of the references would likely receive insufficient +reads, so most of the (many) output XAM files would be useless clutter.

    +

    To remedy this inconvenience, after alignment has finished, XAM files +with fewer than a minimum number of reads are automatically deleted. +The default is 1000, which can be set using the option --min-reads. +Setting --min-reads to 0 disables automatically deleting XAM files.

    +
    How to further customize alignment

    See seismic align for the full list of options that SEISMIC-RNA can @@ -564,6 +579,9 @@

    How to further customize alignment

    Align: output files

    +

    All output files, except FastQC reports, are written into the directory +{out}/{sample}/align, where {out} is the output directory and +{sample} is the name of the sample.

    Align output file: FastQC reports

    If FastQC is run, then it outputs files to {out}/{sample}/qc, where @@ -589,11 +607,9 @@

    Align output file: Alignment maps{out}/{sample}/align/{ref}.{xam}, -where {out} is the output directory (--out-dir), {sample} is -the name of the sample from which the reads came, {ref} is the name -of the reference to which the reads aligned, and {xam} is the file -extension (depending on the selected format). +Each alignment map is written to {ref}.{xam}, where {ref} is the +name of the reference to which the reads aligned, and {xam} is the +file extension (depending on the selected format). SEISMIC-RNA can output alignment maps in either BAM or CRAM format. For a comparison of these formats, see Align option: Format of alignment maps.

    @@ -616,7 +632,7 @@

    Align output file: Reference sequences

    Align output file: Unaligned reads

    In addition to the alignment maps, SEISMIC-RNA outputs FASTQ file(s) of -reads that Bowtie 2 could not align to {out}/{sample}/align:

    +reads that Bowtie 2 could not align:

    • Each whole-sample FASTQ file of single-end (-z) or interleaved (-y) reads yields one file: unaligned.fq.gz

    • @@ -632,24 +648,11 @@

      Align output file: Reference sequences--bt2-no-un.

    -
    -

    Align output file: Align report

    -

    In addition to the alignment maps, SEISMIC-RNA outputs FASTQ file(s) of -reads that Bowtie 2 could not align to {out}/{sample}/align:

    -
      -
    • Each whole-sample FASTQ file of single-end (-z) or interleaved -(-y) reads yields one file: unaligned.fq.gz

    • -
    • Each pair of whole-sample FASTQ files of 1st and 2nd mates (-x) -yields two files: unaligned.fq.1.gz and unaligned.fq.2.gz

    • -
    • Each demultiplexed FASTQ file of single-end (-Z) or interleaved -(-Y) reads yields one file: {ref}__unaligned.fq.gz

    • -
    • Each pair of demultiplexed FASTQ files of 1st and 2nd mates (-X) -yields two files: -{ref}__unaligned.fq.1.gz and {ref}__unaligned.fq.2.gz

    • -
    -

    where {ref} is the reference for demultiplexed FASTQ files.

    -

    Outputting these files of unaligned reads can be disabled using the -option --bt2-no-un.

    +
    +

    Align output file: Report

    +

    A report file, align-report.json, is also written that records the +settings used to run alignment and summarizes the results of alignment. +See Align Report for more information.

    @@ -687,27 +690,22 @@

    Relate input file: Reference sequencesCleaning FASTA Files tool. See FASTA: Reference sequences for details.

    -
    -

    Note

    -

    The references in the FASTA file must match those to which the reads -were aligned to produce the XAM file(s). -A XAM file whose reference is absent from the FASTA will be skipped. -A XAM file whose reference sequence in the FASTA differs from the -sequence to which the XAM file was actually aligned can can yield -erroneous relation vectors or fail to yield any output.

    -

    Relate input file: Alignment maps

    Relate requires one or more alignment map files, each of which must be in SAM, BAM, or CRAM format (collectively, “XAM” format). See SAM, BAM, and CRAM: Alignment Maps for details.

    -
    -
    Alignment map file requirements
    -

    Refer to

    -
    -
    -
    How to relate one or more alignment maps
    +
    +

    Note

    +

    The references in the FASTA file must match those to which the reads +in the alignment map were aligned. +Discrepancies can cause the relate command to fail or produce +erroneous relation vectors. +This problem will not occur if you use the same (unaltered) FASTA +file for both the align and relate commands, or run both +at once using the command seismic all.

    +

    List every alignment map file after the FASTA file. Refer to Listing Input Files for details on how to list multiple files. For example, to compute relation vectors for reads from sample-1 @@ -719,6 +717,93 @@

    How to relate one or more alignment maps{refs.fa} is the path to the file of reference sequences.

    +
    +

    Relate: Options

    +
    +

    Relate options shared with alignment

    +

    Because this workflow can be started from the align or relate +commands, the latter duplicates some of the options of the former: +--phred-enc, --min-mapq, --min-reads, and --out-dir have +the same functions in relate and align.

    +
    +
    +

    Relate option: Minimum Phred score

    +

    Base calls with Phred scores below --min-phred are labeled ambiguous +matches or substitutions, as if they were N``s. +For example, if the minimum Phred score is 25 (the default) and a base +``T is called as a match with a Phred score of 20, then it would be +marked as possibly a match and possibly a subsitution to A, C, or G. +See Relation Vectors for more information.

    +
    +
    +

    Relate option: Ambiguous insertions and deletions

    +

    The most tricky problem in computing relation vectors is that insertions +and deletions (“indels”) in repetitive regions cause ambiguities. +SEISMIC-RNA introduces a new algorithm for identifying ambiguous indels +(see ../algos/ambrel for more information). +This algorithm is enabled by default. +If it is not necessary to identify ambiguous indels, then the algorithm +can be disabled with --no-ambrel, which will speed up relate at +the cost of reducing its accuracy on indels.

    +
    +
    +

    Relate option: Batch size

    +

    For an explanation of batching and how to use it, see Batches and Benefits.

    +

    The dataset is partitioned into batches by the relate command. +The option --batch-size sets a target amount of data for each batch, +in millions of base calls (megabases). +This calculation considers the total number of relationships per read, +which equals the length of the reference sequence. +Thus, the number of base calls B is the product of the number of reads +N and the length of the reference sequence L:

    +

    B = NL

    +

    Since L is known and --batch-size specifies a target size for B, +N can be solved for:

    +

    N = B/L

    +

    SEISMIC-RNA will aim to put exactly N reads in each batch but the last +(the last batch can be smaller because it has just the leftover reads). +If the reads are single-ended or if alignment was not run in mixed mode, +then every batch but the last will contain exactly N reads. +If mixed mode was used, then batches may contain more than N reads, up +to a maximum of 2N in the extreme case that every read in the batch +belonged to a pair in which the other mate did not align.

    +
    +
    +
    +

    Relate: Output files

    +

    All output files go into the directory {out}/{sample}/relate/{ref}, +where {out} is the output directory, {sample} is the sample, and +{ref} is the name of the reference.

    +
    +

    Relate output file: Batch of relation vectors

    +

    The data of relationships is written in batches. +Each batch contains a RelateBatchIO object and is saved to the file +relate-batch-{num}.brickle, where {num} is the batch number. +See Relate Batch for details on the data structure. +See Brickle: Compressed Python Objects for details on brickle files.

    +
    +
    +

    Relate output file: Batch of read names

    +

    For each batch, the relate step assigns an index (a nonnegative integer) +to each read and writes a file mapping the indexes to the read names. +Each batch contains a QnamesBatchIO object and is saved to the file +qnames-batch-{num}.brickle, where {num} is the batch number. +See ../data/relate/qnames for details on the data structure. +See Brickle: Compressed Python Objects for details on brickle files.

    +
    +
    +

    Relate output file: Reference sequence

    +

    The relate step writes the reference sequence as a CompressedSeq +object to the file refseq.brickle. +See ../data/relate/refseq for details on the data structure. +See Brickle: Compressed Python Objects for details on brickle files.

    +
    +
    +

    Relate output file: Report

    +

    A report file is written that records the settings used to generate +relation vectors summarizes the results. +See Relate Report for more information.

    +
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:12,get_invers:12,get_length:12,get_lock:20,get_log_exp_obs_count:9,get_mask:24,get_matrix:9,get_max_uint:10,get_max_valu:10,get_mu:9,get_non_seq_regex:29,get_nonalphaset:24,get_null_valu:41,get_num_parallel:19,get_oconv_array_float:10,get_oconv_dict_float:10,get_oconv_dict_list_float:10,get_oconv_float:10,get_oconv_list_float:10,get_other_iupac:24,get_param_default:11,get_param_lin:11,get_path:[14,31],get_path_field:31,get_path_seg:31,get_pictran:24,get_prop:9,get_quantil:[15,16],get_reads_per_batch:33,get_reads_per_po:12,get_ref_metadata:28,get_refseq_typ:10,get_rel_nam:41,get_rels_per_po:12,get_rels_per_read:12,get_report_typ:[9,10,32,33],get_resp:9,get_sample_data:28,get_sample_metadata:28,get_sample_ref_ext:7,get_sect_coords_prim:24,get_sect_metadata:28,get_seq_base_bar_trac:31,get_seq_base_scatter_trac:31,get_seq_line_trac:31,get_seq_stack_bar_trac:31,get_table1_typ:31,get_table2_typ:31,get_table_data:28,get_table_param:31,get_table_path:9,get_table_typ:31,get_tabulator_writ:41,get_tabulator_writer_typ:41,get_top_logg:10,get_trac:31,get_uint_dtyp:10,get_uint_s:10,get_uint_typ:10,get_uniq_nam:9,get_uniq_read:9,get_verbos:10,github:[0,37,67,76],give:[10,11,14,16,58,62,63,66,70,73],given:[7,9,10,11,13,14,15,17,19,21,24,25,31,33,34,37,38,41,46,48,55,56,57,70,71,73,75],glob:70,global:71,gnu:4,go:[23,43,44,73],goe:[34,66],good:[46,75],googl:0,gpu:67,graph:[6,62],graph_filenam:31,graph_obj:31,graph_typ:31,graphbas:31,graphwrit:31,greater:[7,8,15,25,73],green:10,grep:[27,43,44],grep_both_fastq:27,grote:0,group:[24,73],gs:[7,43,44,59,73],gt_eq:12,gu:[24,44],guess:15,guid:[44,51,65,73,76],guidanc:63,guidelin:77,gz:[41,51,73,75],gzip:[7,41,51],ha:[8,10,15,16,24,33,34,35,37,46,49,50,51,63,66,67,71,73],had:[15,63,67,73],halfrefpattern:21,halfrelpattern:21,halt:71,handl:[68,73],handler:10,happen:[49,71],hard:73,harder:37,harvard:0,has_close_mut:16,has_ipath:13,has_opath:13,hash:24,hashabl:[25,31],have:[4,8,10,15,24,32,37,46,48,51,63,65,66,67,68,70,73],head:73,header:[7,10,17,23,41,46,51,68,73],header_depth:41,header_row:41,header_test:26,header_typ:41,helix:23,help:[15,44,73],helper:41,henc:[46,73],here:48,hex:46,hexadecim:[14,46],hh:71,hi_qual:34,hide:66,high:[7,15,34,43,44,46,59,73],higher:71,highest:46,highli:66,histori:37,home:[63,65],hook:65,hope:4,hopefulli:77,horizont:63,how:[19,23,46,62,64,65,68,69],howev:[46,48,70,73],ht:37,html:[30,31,44,63,65,73],htslib:63,http:[4,9,15,30,37,63,65,75,77],hui:57,human:[34,55],humanize_relvec:34,hundr:73,hybrid:19,hyphen:[24,25],hyphenate_end:24,i:[9,10,15,19,21,24,31,34,35,44,48,50,51,53,68,70,73],iconv:10,iconv_array_float:10,iconv_array_int:10,iconv_datetim:10,iconv_dict_str_int:10,iconv_int_kei:10,idea:[66,75],ideal:[10,46],ident:[9,25,46],identifi:[37,73],idxstats_cmd:17,ifasta:29,ignor:[24,25,32,43,44,50,71],iii:73,illeg:[25,50,68],illumina:51,illustr:[46,51],imag:77,immedi:[46,71],implement:66,impli:4,implicitli:[37,70],impos:50,imposs:46,improperli:73,improv:[10,67,73],inadvert:75,includ:[9,15,24,44,50,55,58,63,67],inclus:58,incompat:66,inconsist:[10,50],inconveni:73,incorpor:50,incorrect:[51,73],incorrectli:51,increas:[43,44,73],increment:48,incur:73,indel:[7,8,33,37,43,44,46,59,73],independ:[9,15,43,44],index:[7,9,10,12,15,17,24,25,27,30,31,32,33,34,37,41,43,44,48,50,58,63],index_clust:10,index_col:41,index_depth:41,index_fasta_cmd:17,index_ord:10,index_order_clust:10,index_orders_clust:10,index_pfx:7,index_to_po:[24,25],index_to_seq:[24,25],index_toler:[27,43,44],index_xam_cmd:17,indic:[19,37,46,51,68,73],indiv:44,individu:[0,7,31,43,44,59,63,70,73],inequ:[7,8],infer:[11,34,41,56,57],infer_read:[34,35],infer_report_loader_typ:41,infer_test:35,info:[42,71],inform:[9,10,32,37,38,43,44,46,48,49,50,51,53,55,67,70,73],infrequ:37,inher:73,init:[32,65,73],initi:[7,15,37,42,43,44,50,59,64,68,73],inn:37,input:[7,13,62,67,68,69],input_csv:27,input_path:[9,28,30,31,32,33,41,43,44],ins:44,ins_len:34,ins_po:37,insensit:21,insert:[7,8,21,32,33,34,35,37,38,43,44],insid:16,inspect:11,instal:[62,66],instanc:[9,10,21,24,25,35],instanti:[10,25],instead:[10,63,66,73],instruct:[63,65,73],instrument:73,insuffici:[37,73],integ:[8,10,12,17,24,33,34,37,48,51,56,57,73],integr:75,interchang:73,interest:[41,49,71],interfac:[6,7,10,33,42,62,69,76],interleav:[7,17,43,44,53],intermediari:7,interpret:71,interrupt:71,intersect:[21,24],interv:[7,43,44,59],introduc:[7,73],introduct:[2,7],invalid:[10,16,25,35,68,73],invent:[1,66],invers:[7,21],invert:[16,21,24],involv:10,io:[6,9,10,32,33,37,65],iopath:13,ipath:13,irrelev:53,is_ext:10,issu:[9,51,66,67,75],item:[9,10,14,19,24,28,31],iter:[7,9,10,12,14,15,19,21,23,24,27,31,32,33,35,38,41,43,44],iter_align:34,iter_base_typ:12,iter_batch:[10,33],iter_cas:38,iter_clust_table_data:28,iter_pos_table_data:28,iter_pos_table_seri:28,iter_pos_table_struct:28,iter_profil:41,iter_read:12,iter_read_table_data:28,iter_record:33,iter_relvecs_al:[34,35],iter_relvecs_q53:[34,35],iter_root_modul:23,iter_seq_base_bar_trac:31,iter_seq_base_scatter_trac:31,iter_seq_line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package","seismicrna.align package","seismicrna.align.tests package","seismicrna.clust package","seismicrna.core package","seismicrna.core.arg package","seismicrna.core.batch package","seismicrna.core.extern package","seismicrna.core.io package","seismicrna.core.mu package","seismicrna.core.mu.tests package","seismicrna.core.ngs package","seismicrna.core.ngs.tests package","seismicrna.core.parallel package","seismicrna.core.parallel.tests package","seismicrna.core.rel package","seismicrna.core.rel.tests package","seismicrna.core.rna package","seismicrna.core.seq package","seismicrna.core.seq.tests package","seismicrna.core.tests package","seismicrna.demult package","seismicrna.export package","seismicrna.fastaclean package","seismicrna.fold package","seismicrna.graph package","seismicrna.mask package","seismicrna.relate package","seismicrna.relate.aux package","seismicrna.relate.aux.tests package","seismicrna.relate.c package","seismicrna.relate.py package","seismicrna.relate.py.tests package","seismicrna.sim package","seismicrna.sim.tests package","seismicrna.table package","seismicrna.test package","seismicrna.workflow package","Command Line Interface","Data Structures","Relation Vectors","Relate Data Structures","Relate Batch","Brickle: Compressed Python Objects","FASTA: Reference sequences","FASTQ: Sequencing Reads","Data Formats","SAM, BAM, and CRAM: Alignment Maps","File Formats","Metadata Formats","Metadata for References","Metadata for Samples","Metadata for Sections","Align Report","Report Formats","Relate Report","SEISMIC-RNA User Guide","Installing with Conda","Installation","Prerequisites: Python, pip, and Conda","Virtual Environments","Bugs and Requests","Cleaning FASTA Files","Manuals","Listing Input Files","Logging Messages","Parallelizing Tasks","Running the Workflow","Step-by-step walkthrough","Example dataset","Tutorials","How to Write this 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\ No newline at end of file diff --git a/docs/writeme.html b/docs/writeme.html index 83d1903e..58c0b956 100644 --- a/docs/writeme.html +++ b/docs/writeme.html @@ -83,14 +83,14 @@ diff --git a/src/userdocs/data/index.rst b/src/userdocs/data/index.rst index de902f1f..3f5855ae 100644 --- a/src/userdocs/data/index.rst +++ b/src/userdocs/data/index.rst @@ -6,4 +6,4 @@ Data Structures .. toctree:: :maxdepth: 2 - relate + relate/index diff --git a/src/userdocs/data/relate.rst b/src/userdocs/data/relate.rst deleted file mode 100644 index 04ab01c5..00000000 --- a/src/userdocs/data/relate.rst +++ /dev/null @@ -1,234 +0,0 @@ - -Relation Vectors ------------------------------------------------------------------------- - -Relationships between reads and references -^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ - -A relation vector encodes the relationship between one sequencing read -(or pair of mated reads) and each base in the reference sequence. -SEISMIC-RNA defines eight primary relationships: - -- **Match**: The reference base aligned to a high-quality base of the same kind in the read. -- **Deletion**: The reference base aligned to the space between two bases in the read. -- **5' of Insertion**: The reference base aligned to any read base immediately 5' of an extra base in the read. -- **3' of Insertion**: The reference base aligned to any read base immediately 3' of an extra base in the read. -- **Substitution to A**: The reference base is not A and aligned to a high-quality A in the read. -- **Substitution to C**: The reference base is not C and aligned to a high-quality C in the read. -- **Substitution to G**: The reference base is not G and aligned to a high-quality G in the read. -- **Substitution to T**: The reference base is not T and aligned to a high-quality T in the read. - -This figure illustrates six of these primary relationships, as well the -"blank" relationship for positions in the reference that lie outside the -span of the read. - -.. image:: - relationships.png - -Encoding primary relationships -^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ - -Each position in a relation vector is physically represented by one byte -(eight bits); each bit corresponds to one of the eight types of primary -relationship. For a given position in the relation vector, the byte(s) -corresponding to the relationship(s) at that position are turned on (set -to 1); all other bits are set to 0. The following table indicates which -relationship each bit represents. Each bit is shown as the sole 1 within -an entire byte, an eight-digit binary number. The number's decimal (Dec) -and hexadecimal (Hex) forms are also shown. - -========== ===== ===== =================== - Byte Dec Hex Relationship -========== ===== ===== =================== - 00000001 001 01 Match - 00000010 002 02 Deletion - 00000100 004 04 5' of Insertion - 00001000 008 08 3' of Insertion - 00010000 016 10 Substitution to A - 00100000 032 20 Substitution to C - 01000000 064 40 Substitution to G - 10000000 128 80 Substitution to T -========== ===== ===== =================== - -Encoding ambiguous relationships -^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ - -Oh, if only encoding relation vectors were that straightforward! Though -most bases in a read will have primary relationships with the bases in -the reference to which they align, two phenomena make it more difficult -for some bases to define the relationship: - -- low-quality base calls -- ambiguous insertions and deletions - -Low-quality base calls -"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" - -A low-quality base call is defined as having a `Phred quality score`_ -below the user-specified threshold (default: 25). Low-quality base calls -are processed as if they could be any of the four nucleotides. The type -of relationship that would occur if the read base were each of the four -nucleotides is determined. The bytes for those relationships are united -via `bitwise OR`_ into a consensus byte for the ambiguous relationship. - -For example, suppose a low-quality base call in the read aligns to a T -in the reference. If the read base (which is unknown because of its low -quality) were actually A, then the relationship would be a substitution -to A (``00010000``). Likewise if the read base were C (``00100000``) or -G (``01000000``). If the read base were T, then the relationship would -be a match (``00000001``). The bytes for these four possible situations -are merged by taking the bitwise OR into a consensus byte (``01110001``) -that expresses the ambiguity in the relationship. This consensus byte is -inserted into the relation vector. - -============== ============================ ========== ===== ===== - If read were Then relationship would be Byte Dec Hex -============== ============================ ========== ===== ===== - A Substitution to A 00010000 016 10 - C Substitution to C 00100000 032 20 - G Substitution to G 01000000 064 40 - T Match 00000001 001 01 - Low-quality Any of the above 01110001 113 71 -============== ============================ ========== ===== ===== - -Each row of the following table repeats this calculation for one type of -reference base (Ref). Each column "Read: A/C/G/T?" shows what would be -the relationship if the low-quality base in the read were actually the -base in the column header. For example, in the first row, the reference -base is A: if the read base were A (first column), then the relationship -would be a match (``00000001``); and if it were C (second column), then -the relationship would be a substitution to C (``00100000``). The column -"Byte" shows the resulting ambiguous relationship, the bitwise OR of the -four columns "Read: A/C/G/T?". - -===== ========== ========== ========== ========== ========== ===== ===== - Ref Read: A? Read: C? Read: G? Read: T? Byte Dec Hex -===== ========== ========== ========== ========== ========== ===== ===== - A 00000001 00100000 01000000 10000000 11100001 225 e1 - C 00010000 00000001 01000000 10000000 11010001 209 d1 - G 00010000 00100000 00000001 10000000 10110001 177 b1 - T 00010000 00100000 01000000 00000001 01110001 113 71 -===== ========== ========== ========== ========== ========== ===== ===== - -.. note:: - A byte that has more than one bit set to 1 does **not** count more - than once towards the total number of matches or mutations. To learn - how mutations in relation vectors are counted, see [REF]. - -Ambiguous insertions and deletions -"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" - -Insertions and deletions (collectively, "indels") in the read can cause -ambiguities that even the highest quality sequencing reads could not -prevent. When one or more bases are inserted or deleted in a repetitive -sequence, the exact base that mutated cannot be determined. For example, -if the reference is ``ATCCTG`` and the read is ``ATCTG``, then one C was -clearly deleted from the read. But determining whether it was the first -or second C is impossible because the alignments are equally good: - -**Deletion of the first C** :: - - AT-CTG - || ||| - ATCCTG - -**Deletion of the second C** :: - - ATC-TG - ||| || - ATCCTG - -Ambiguities in the location of a relationship are encoded by turning on -the bit of every possible relationship at each position. In the above -example, position 3 of the reference could be a deletion (``00000010``) -or a match (``00000001``), so the byte it receives is the bitwise OR of -the two relationships: ``00000011``. Likewise for position 4. Thus, the -relationship byte at each position (Pos) in the alignment would be - -===== ========== ===== - Pos Byte Hex -===== ========== ===== - 1 00000001 01 - 2 00000001 01 - 3 00000011 03 - 4 00000011 03 - 5 00000001 01 - 6 00000001 01 -===== ========== ===== - -.. note:: - A byte that has more than one bit set to 1 does **not** count more - than once towards the total number of matches or mutations. To learn - how mutations in relation vectors are counted, see [REF]. - -To learn how the algorithm that finds ambiguous indels works, see -[REF]. - -Encoding positions not covered by the read -^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ - -If a read is shorter than the reference, then it will not align to every -position in the reference. The "blank" positions to which it does not -align provide no information and are thus considered fully ambiguous and -assigned the byte ``11111111`` (decimal 255, hexadecimal ff). - -Encoding paired-end reads -^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ - -For paired-end reads, both mates produce a relation vector. They must be -merged into one consensus relation vector to avoid double-counting any -positions where the two mates overlap. Ideally, the mates would have -identical relationships. However, they often differ because a position -is covered in one mate but not in the other, one mate's Phred score is -above the threshold and the other's is below, or (more rarely) the base -calls themselves differ. - -Consensus relationships -"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" - -When finding the consensus of two mates, information in one mate should -compensate for a lack thereof in the other. Recall that a relationship -byte indicates all possible relationships at its position. The more bits -that are set to 1, the more ambiguity (and the less knowledge) there is -about the relationship. For one mate to add knowledge to the other, the -consensus byte must thus have no more 1s than the byte of either mate. -Specifically, a bit in the consensus should be 1 only if it is 1 in both -mates. This operation is `bitwise AND`_. - -For example, consider the following mate 1 and mate 2, where the column -"Result" indicates the consensus byte after taking the bitwise AND: - -===== ========== ========== ========== - Pos Mate 1 Mate 2 Result -===== ========== ========== ========== - 1 00000001 00000001 00000001 - 2 00000001 11010001 00000001 - 3 11100001 01000000 01000000 - 4 11111111 00000001 00000001 - 5 11111111 01110001 01110001 - 6 11111111 11111111 11111111 -===== ========== ========== ========== - -At position 1, the mates agree on a match. At position 2, mate 2 has low -quality, but mate 1 compensates with a high-quality match, so that the -result has only the match bit set to 1. Similarly, at position 3, a -substitution to G in mate 2 compensates for the low quality base call in -mate 1: substitution to G is the consensus. Mate 1 does not cover the -positions 4-6 (hence the blank bytes ``11111111``). Mate 2 informs that -position 4 is a match, but it is low quality at position 5, so even the -consensus byte is ambiguous. Neither mate covers position 6, so the -consensus byte is blank. - -Irreconcilable relationships -"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" - -It is possible, although rare, for mates 1 and 2 to share no bits. For -example, if mate 1 were a high-quality match (``00000001``) and mate 2 -were a high-quality substitution to T (``10000000``), then the bitwise -AND would be all zeros (``00000000``). The mates would be irreconcilable -at this position. - - -.. _Phred quality score: https://en.wikipedia.org/wiki/Phred_quality_score -.. _bitwise OR: https://en.wikipedia.org/wiki/Bitwise_operation#OR -.. _bitwise AND: https://en.wikipedia.org/wiki/Bitwise_operation#AND diff --git a/src/userdocs/data/relate/index.rst b/src/userdocs/data/relate/index.rst new file mode 100644 index 00000000..cb44c818 --- /dev/null +++ b/src/userdocs/data/relate/index.rst @@ -0,0 +1,9 @@ + +Relate Data Structures +======================================================================== + +.. toctree:: + :maxdepth: 2 + + codes + relate diff --git a/src/userdocs/data/relate/relate.rst b/src/userdocs/data/relate/relate.rst new file mode 100644 index 00000000..92ce4f97 --- /dev/null +++ b/src/userdocs/data/relate/relate.rst @@ -0,0 +1,117 @@ + +Relate Batch +------------------------------------------------------------------------ + +Each batch of relation vectors is a ``RelateBatchIO`` object. + +Relate batch: Specifications +^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ + +The following attributes encode the relationships between each read and +each position in the reference sequence: + +========= ============================================ ==================================================================== +Attribute Data Type Description +========= ============================================ ==================================================================== +``end5s`` ``numpy.ndarray[int]`` array of the first position of the most upstream mate in each read +``mid5s`` ``numpy.ndarray[int]`` array of the first position of the most downstream mate in each read +``mid3s`` ``numpy.ndarray[int]`` array of the last position of the most upstream mate in each read +``end3s`` ``numpy.ndarray[int]`` array of the last position of the most downstream mate in each read +``muts`` ``dict[int, dict[int, numpy.ndarray[int]]]`` array of the reads with each type of mutation at each position +========= ============================================ ==================================================================== + +.. note:: + The positions of the first and last bases in the reference sequence + are defined to be 1 and the length of the sequence, respectively. + +Relate batch: Specifications of read numbers +"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" + +Each read, or pair of paired-end reads, is labeled with a non-negative +integer: 0 for the first read in each batch, and incrementing by 1 for +each subsequent read. +Within one batch, all read numbers are unique. +However, two different batches can have reads that share numbers. + +Relate batch: Specifications of 5' and 3' end positions +"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" + +- ``end5s``, ``mid5s``, ``mid3s``, and ``end3s`` are all 1-dimensional + ``numpy.ndarray`` objects. +- For any relate batch, ``end5s``, ``mid5s``, ``mid3s``, and ``end3s`` + all have the same length (which may be any integer ≥ 0). +- A read with index ``i`` corresponds to the ``i``\ th values of + ``end5s``, ``mid5s``, ``mid3s``, and ``end3s``; denoted (respectively) + ``end5s[i]``, ``mid5s[i]``, ``mid3s[i]``, and ``end3s[i]``. +- For every read ``i``: + + - 1 ≤ ``end5s[i]`` ≤ ``end3s[i]`` ≤ length of reference sequence + - If paired-end and there is a gap of ≥ 1 nt between mates 1 and 2: + + - ``end5s[i]`` ≤ ``mid3s[i]`` < ``mid5s[i]`` ≤ ``end3s[i]`` + + - Otherwise: + + - ``end5s[i]`` = ``mid5s[i]`` ≤ ``mid3s[i]`` = ``end3s[i]`` + +Relate batch: Specifications of mutations +"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" + +``muts`` is a ``dict`` wherein + +- each key is a position in the reference sequence (``int``) +- each value is a ``dict`` wherein + + - each key is a type of mutation (``int``, see :doc:`./codes` for more + information) + - each value is an array of the numbers of the reads that have the + given type of mutation at the given position (``numpy.ndarray``) + +Relate batch: Example +^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ + +For example, suppose that the reference sequence is ``TCAGAACC`` and a +batch contains five paired-end reads, numbered 0 to 4: + +==== ====== ====== ====== ====== +Read 5' End 5' Mid 3' Mid 3' End +==== ====== ====== ====== ====== +0 2 4 6 8 +1 1 1 6 6 +2 2 3 5 7 +3 1 5 7 8 +4 3 3 7 7 +==== ====== ====== ====== ====== + +The positions, reads, and relationships can be shown explicitly as a +matrix (see :doc:`./codes` for an explanation of the relationships): + +==== === === === === === === === === +Read 1 2 3 4 5 6 7 8 +==== === === === === === === === === +0 255 1 1 1 255 1 16 1 +1 1 1 128 1 128 1 255 255 +2 255 1 1 255 1 1 1 255 +3 1 64 1 1 128 255 1 1 +4 255 255 1 1 3 3 1 255 +==== === === === === === === === === + +In a relate batch, they would be encoded as follows: + +- ``end5s``: ``[2, 1, 2, 1, 3]`` +- ``mid5s``: ``[4, 1, 3, 5, 3]`` +- ``mid3s``: ``[6, 6, 5, 7, 7]`` +- ``end3s``: ``[8, 6, 7, 8, 7]`` +- ``muts``:: + + {1: {}, + 2: {64: [3]}, + 3: {128: [1]}, + 4: {}, + 5: {3: [4], 128: [1, 3]}, + 6: {3: [4]}, + 7: {16: [0]}, + 8: {} + + Note that the numbers are shown here for visual simplicity as ``list`` + objects, but would really be ``numpy.ndarray`` objects. diff --git a/src/userdocs/data/relationships.pdf b/src/userdocs/data/relate/relationships.pdf similarity index 100% rename from src/userdocs/data/relationships.pdf rename to src/userdocs/data/relate/relationships.pdf diff --git a/src/userdocs/data/relationships.png b/src/userdocs/data/relate/relationships.png similarity index 100% rename from src/userdocs/data/relationships.png rename to src/userdocs/data/relate/relationships.png diff --git a/src/userdocs/formats/data/fastq.rst b/src/userdocs/formats/data/fastq.rst index b91570d4..d81c6b4a 100644 --- a/src/userdocs/formats/data/fastq.rst +++ b/src/userdocs/formats/data/fastq.rst @@ -33,10 +33,10 @@ file was called correctly during sequencing. The probability *p* that a base was called incorrectly is 10 raised to the power of the quotient of the Phred score *s* and -10: -*p* = 10 :sup:`-s/10` +*p* = 10\ :sup:`-s/10` For example, if a base call has a Phred score of 30, the probability -that the base call is incorrect is 10 :sup:`-30/10` = 0.001. +that the base call is incorrect is 10\ :sup:`-30/10` = 0.001. In FASTQ files, each phred quality score (a non-negative integer) is encoded as one character of text by adding another integer *N* to the diff --git a/src/userdocs/manuals/workflow.rst b/src/userdocs/manuals/workflow.rst index 6cc63110..fe7b5bc1 100644 --- a/src/userdocs/manuals/workflow.rst +++ b/src/userdocs/manuals/workflow.rst @@ -246,7 +246,7 @@ How to trim low-quality base calls Base calls on either end of a read that fall below a minimum Phred score quality are trimmed with Cutadapt. The default minimum quality is 25, which corresponds to a probability of -1 - 10 :sup:`-2.5` = 0.997 that the base call is correct. +1 - 10\ :sup:`-2.5` = 0.997 that the base call is correct. (See :ref:`phred_encodings` for more details). To change the quality threshold, use the option ``--min-phred``. @@ -385,7 +385,7 @@ on setting this parameter. the read aligns with a high score to exactly one location, low quality if it aligns with similar scores to multiple locations in the reference. The default minimum quality is 25, which corresponds to a confidence of -1 - 10 :sup:`-2.5` = 0.997 that the read has aligned correctly. +1 - 10\ :sup:`-2.5` = 0.997 that the read has aligned correctly. To change the quality threshold, use the option ``--min-mapq``. For those searching for this option in Bowtie 2, you will not find it. Instead, reads with insufficient mapping quality are filtered out after @@ -416,7 +416,6 @@ If you require a more customized alignment workflow, you can align your your FASTQ files outside of SEISMIC-RNA, then pass the resulting XAM files into SEISMIC-RNA at the step :ref:`wf_relate`. - .. _bam_vs_cram: Align option: Format of alignment maps @@ -464,6 +463,10 @@ that accompanies them). Align: output files ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ +All output files, except FastQC reports, are written into the directory +``{out}/{sample}/align``, where ``{out}`` is the output directory and +``{sample}`` is the name of the sample. + Align output file: FastQC reports """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" @@ -492,11 +495,9 @@ each read aligned, as well as the Phred quality scores, mapping quality, and mutated positions. SEISMIC-RNA outputs alignment maps where every read aligns to the same reference (although this is not a restriction outside of SEISMIC-RNA). -Each alignment map is written to ``{out}/{sample}/align/{ref}.{xam}``, -where ``{out}`` is the output directory (``--out-dir``), ``{sample}`` is -the name of the sample from which the reads came, ``{ref}`` is the name -of the reference to which the reads aligned, and ``{xam}`` is the file -extension (depending on the selected format). +Each alignment map is written to ``{ref}.{xam}``, where ``{ref}`` is the +name of the reference to which the reads aligned, and ``{xam}`` is the +file extension (depending on the selected format). SEISMIC-RNA can output alignment maps in either BAM or CRAM format. For a comparison of these formats, see :ref:`bam_vs_cram`. @@ -523,7 +524,7 @@ Align output file: Unaligned reads """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" In addition to the alignment maps, SEISMIC-RNA outputs FASTQ file(s) of -reads that Bowtie 2 could not align to ``{out}/{sample}/align``: +reads that Bowtie 2 could not align: - Each whole-sample FASTQ file of single-end (``-z``) or interleaved (``-y``) reads yields one file: ``unaligned.fq.gz`` @@ -543,8 +544,8 @@ option ``--bt2-no-un``. Align output file: Report """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" -A report file is written that records the settings used to run alignment -and summarizes the results of alignment. +A report file, ``align-report.json``, is also written that records the +settings used to run alignment and summarizes the results of alignment. See :doc:`../formats/report/align` for more information. Align: Troubleshooting @@ -627,14 +628,12 @@ the same functions in ``relate`` and ``align``. Relate option: Minimum Phred score """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" -Like ``align``, ``relate`` also has the option ``--min-phred``, but its -meaning is different than that during the ``align`` step. -In ``relate``, base calls with Phred scores below ``--min-phred`` are -considered ambiguous matches or substitutions, as if they were ``N``s. +Base calls with Phred scores below ``--min-phred`` are labeled ambiguous +matches or substitutions, as if they were ``N``s. For example, if the minimum Phred score is 25 (the default) and a base ``T`` is called as a match with a Phred score of 20, then it would be marked as possibly a match and possibly a subsitution to A, C, or G. -See :doc:`../data/relate` for more information. +See :doc:`../data/relate/codes` for more information. Relate option: Ambiguous insertions and deletions """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" @@ -673,16 +672,42 @@ SEISMIC-RNA will aim to put exactly *N* reads in each batch but the last If the reads are single-ended or if alignment was not run in mixed mode, then every batch but the last will contain exactly *N* reads. If mixed mode was used, then batches may contain more than *N* reads, up -to a maximum of 2 *N* in the extreme case that every read in the batch +to a maximum of 2\ *N* in the extreme case that every read in the batch belonged to a pair in which the other mate did not align. Relate: Output files ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ +All output files go into the directory ``{out}/{sample}/relate/{ref}``, +where ``{out}`` is the output directory, ``{sample}`` is the sample, and +``{ref}`` is the name of the reference. + Relate output file: Batch of relation vectors """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" +The data of relationships is written in batches. +Each batch contains a ``RelateBatchIO`` object and is saved to the file +``relate-batch-{num}.brickle``, where ``{num}`` is the batch number. +See :doc:`../data/relate/relate` for details on the data structure. +See :doc:`../formats/data/brickle` for details on brickle files. + +Relate output file: Batch of read names +"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" + +For each batch, the relate step assigns an index (a nonnegative integer) +to each read and writes a file mapping the indexes to the read names. +Each batch contains a ``QnamesBatchIO`` object and is saved to the file +``qnames-batch-{num}.brickle``, where ``{num}`` is the batch number. +See :doc:`../data/relate/qnames` for details on the data structure. +See :doc:`../formats/data/brickle` for details on brickle files. + +Relate output file: Reference sequence +"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" +The relate step writes the reference sequence as a ``CompressedSeq`` +object to the file ``refseq.brickle``. +See :doc:`../data/relate/refseq` for details on the data structure. +See :doc:`../formats/data/brickle` for details on brickle files. Relate output file: Report """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" diff --git a/src/userdocs/writeme.rst b/src/userdocs/writeme.rst index db100b72..9d55f67c 100644 --- a/src/userdocs/writeme.rst +++ b/src/userdocs/writeme.rst @@ -4,11 +4,11 @@ How to Write this Manual .. figure:: https://imgs.xkcd.com/comics/manuals.png :align: center - :alt: A comic strip from xkcd joking that the most unhelpful tool is + :alt: A comic strip of xkcd joking that the most unhelpful tool is one whose manual starts with "How to read this manual". Image credit: "Manuals" from xkcd by Randall Munroe (https://xkcd.com/1343/) -Rather than begin with "How to read this manual" (hopefully, this manual -is sufficiently self-evident in that regard), this manual concludes with -guidelines for writing and editing future versions. +Rather than begin with "How to read this manual" (hopefully, that task +is sufficiently self-evident), this documentation ends with guidelines +for writing future versions. From ef85dc848309c648980eaee3bedbbea11131ec6a Mon Sep 17 00:00:00 2001 From: matthewfallan <31744230+matthewfallan@users.noreply.github.com> Date: Thu, 16 Nov 2023 16:51:36 -0500 Subject: [PATCH 3/9] Update relate batch docs --- docs/.doctrees/data/relate/index.doctree | Bin 2793 -> 2845 bytes docs/.doctrees/data/relate/qnames.doctree | Bin 0 -> 10677 bytes docs/.doctrees/data/relate/refseq.doctree | Bin 0 -> 13105 bytes docs/.doctrees/data/relate/relate.doctree | Bin 44257 -> 46563 bytes docs/.doctrees/environment.pickle | Bin 2983258 -> 2987457 bytes docs/.doctrees/manuals/workflow.doctree | Bin 171396 -> 171563 bytes docs/_sources/data/relate/index.rst.txt | 2 + docs/_sources/data/relate/qnames.rst.txt | 39 ++++ docs/_sources/data/relate/refseq.rst.txt | 48 +++++ docs/_sources/data/relate/relate.rst.txt | 48 +++-- docs/_sources/manuals/workflow.rst.txt | 22 +- docs/algos/index.html | 4 +- docs/data/index.html | 2 + docs/data/relate/codes.html | 2 + docs/data/relate/index.html | 14 +- docs/data/relate/qnames.html | 184 +++++++++++++++++ docs/data/relate/refseq.html | 191 ++++++++++++++++++ docs/data/relate/relate.html | 102 +++++----- docs/manuals/workflow.html | 56 +++--- docs/objects.inv | Bin 23984 -> 24130 bytes docs/searchindex.js | 2 +- src/userdocs/data/relate/codes.rst | 234 ++++++++++++++++++++++ src/userdocs/data/relate/index.rst | 2 + src/userdocs/data/relate/qnames.rst | 39 ++++ src/userdocs/data/relate/refseq.rst | 48 +++++ src/userdocs/data/relate/relate.rst | 46 +++-- src/userdocs/manuals/workflow.rst | 22 +- 27 files changed, 969 insertions(+), 138 deletions(-) create mode 100644 docs/.doctrees/data/relate/qnames.doctree create mode 100644 docs/.doctrees/data/relate/refseq.doctree create mode 100644 docs/_sources/data/relate/qnames.rst.txt create mode 100644 docs/_sources/data/relate/refseq.rst.txt create mode 100644 docs/data/relate/qnames.html create mode 100644 docs/data/relate/refseq.html create mode 100644 src/userdocs/data/relate/codes.rst 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  • Relate Data Structures
  • @@ -102,10 +104,20 @@

    Relate Data Structures
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  • Read Names Batch +
  • +
  • Reference Sequence +
  • Algorithms - + diff --git a/docs/data/index.html b/docs/data/index.html index 9e84a59c..c7dd48fc 100644 --- a/docs/data/index.html +++ b/docs/data/index.html @@ -91,6 +91,8 @@

    How to Write this Manual

    -A comic strip from xkcd joking that the most unhelpful tool is one whose manual starts with "How to read this manual". +A comic strip of xkcd joking that the most unhelpful tool is one whose manual starts with "How to read this manual".

    Image credit: “Manuals” from xkcd by Randall Munroe (https://xkcd.com/1343/)

    -

    Rather than begin with “How to read this manual” (hopefully, this manual -is sufficiently self-evident in that regard), this manual concludes with -guidelines for writing and editing future versions.

    +

    Rather than begin with “How to read this manual” (hopefully, that task +is sufficiently self-evident), this documentation ends with guidelines +for writing future versions.

    diff --git a/docs/data/relate/qnames.html b/docs/data/relate/qnames.html new file mode 100644 index 00000000..62bbac93 --- /dev/null +++ b/docs/data/relate/qnames.html @@ -0,0 +1,184 @@ + + + + + + + Read Names Batch — seismic-rna documentation + + + + + + + + + + + + + + + + + +
    + + +
    + +
    +
    +
    + +
    +
    +
    +
    + +
    +

    Read Names Batch

    +

    Each batch of relation vectors is a QnamesBatchIO object.

    +
    +

    Read names batch: Structure

    +

    The read names are in the attribute names, a numpy.ndarray with +data type str. +The name of the read with number i (see Relate batch: Structure of read numbers for +more information) is at index i of the names array.

    +
    +
    +

    Read names batch: Example

    +

    Suppose a XAM file contains five reads:

    + ++++ + + + + + + + + + + + + + + + + + + + + + + +

    Read Number

    Read Name

    0

    VL00355:66:AACYHM7M5:1:2106:54095:30496

    1

    VL00355:66:AACYHM7M5:1:2303:34554:55259

    2

    VL00355:66:AACYHM7M5:1:2411:30558:38352

    3

    VL00355:66:AACYHM7M5:1:1206:41825:52949

    4

    VL00355:66:AACYHM7M5:1:2506:10903:12605

    +

    The relate step would write a batch with the names attribute

    +
    ["VL00355:66:AACYHM7M5:1:2106:54095:30496",
    + "VL00355:66:AACYHM7M5:1:2303:34554:55259",
    + "VL00355:66:AACYHM7M5:1:2411:30558:38352",
    + "VL00355:66:AACYHM7M5:1:1206:41825:52949",
    + "VL00355:66:AACYHM7M5:1:2506:10903:12605"]
    +
    +
    +

    Note that the attribute is shown as a list for visual simplicity, +but would really be a numpy.ndarray.

    +
    +
    + + +
    +
    + +
    +
    +
    +
    + + + + \ No newline at end of file diff --git a/docs/data/relate/refseq.html b/docs/data/relate/refseq.html new file mode 100644 index 00000000..42b46670 --- /dev/null +++ b/docs/data/relate/refseq.html @@ -0,0 +1,191 @@ + + + + + + + Reference Sequence — seismic-rna documentation + + + + + + + + + + + + + + + + + +
    + + +
    + +
    +
    +
    + +
    +
    +
    +
    + +
    +

    Reference Sequence

    +

    The reference sequence is saved as a RefseqIO object.

    +
    +

    Reference sequence: Structure

    +

    A RefseqIO object stores a reference sequence as a space-efficient +CompressedSeq object in the private attribute _s. +The original sequence is available via the cached property refseq.

    +

    In a standard sequence, each base is represented by one character, and +hence occupies 1 byte (8 bits). +But because each base has only 4 possibilities, each base requires only +log2(4) = 2 bits, shown in the following table:

    + ++++ + + + + + + + + + + + + + + + + + + + +

    Base

    Binary Code

    A

    00

    C

    01

    G

    10

    T

    11

    +

    A CompressedSeq object uses this code to compress 4 bases +(= 8 bits/byte ÷ 2 bits/nt) into each byte. +Each has the following attributes:

    +
      +
    • b (bytes): sequence of bytes, each byte encoding 4 nucleotides

    • +
    • s (int): length of the sequence (number of nucleotides)

    • +
    • n (tuple[int, ...]): 0-indexed position of each N

    • +
    • r (bool): True if the sequence is RNA, False if DNA

    • +
    +
    +
    +

    Reference sequence: Example

    +

    Suppose that a DNA sequence is CAGNTTCGAN. +This sequence would be compressed into

    +
    b = b'!\x9f\x00'
    +s = 10
    +n = (3, 9)
    +r = False
    +
    +
    +

    For details on the algorithm, see ../../algos/compseq.

    +
    +
    + + +
    +
    + +
    +
    +
    +
    + + + + \ No newline at end of file diff --git a/docs/data/relate/relate.html b/docs/data/relate/relate.html index a3778ae0..da176cfe 100644 --- a/docs/data/relate/relate.html +++ b/docs/data/relate/relate.html @@ -19,7 +19,7 @@ - + @@ -55,10 +55,12 @@
  • Relate Data Structures
  • @@ -97,8 +99,8 @@

    Relate Batch

    Each batch of relation vectors is a RelateBatchIO object.

    -
    -

    Relate batch: Specifications

    +
    +

    Relate batch: Structure

    The following attributes encode the relationships between each read and each position in the reference sequence:

    @@ -141,16 +143,16 @@

    Relate batch: Specifications -

    Relate batch: Specifications of read numbers

    +
    +

    Relate batch: Structure of read numbers

    Each read, or pair of paired-end reads, is labeled with a non-negative integer: 0 for the first read in each batch, and incrementing by 1 for each subsequent read. Within one batch, all read numbers are unique. However, two different batches can have reads that share numbers.

    -
    -

    Relate batch: Specifications of 5’ and 3’ end positions

    +
    +

    Relate batch: Structure of 5’ and 3’ end positions

    • end5s, mid5s, mid3s, and end3s are all 1-dimensional numpy.ndarray objects.

    • @@ -176,8 +178,8 @@

      Relate batch: Specifications of 5’ and 3’ end positions -

      Relate batch: Specifications of mutations

      +
      +

      Relate batch: Structure of mutations

      muts is a dict wherein

      • each key is a position in the reference sequence (int)

      • @@ -198,55 +200,65 @@

        Relate batch: Example

    -----+++ - - - - + + + + + + - - - + - + - - - + + + + + + + + + - - - + - + - - - + + + + + - - - - + + + + + + + + + +

    Read

    5’ End

    5’ Mid

    3’ Mid

    3’ End

    Mate

    Alignment

    0

    1

    _CAG____

    0

    2

    4

    6

    8

    _____AGC

    1

    1

    1

    1

    6

    6

    ___GTA__

    1

    2

    TCT_____

    2

    1

    ____AAC_

    2

    2

    3

    5

    7

    _CA_____

    3

    3

    1

    5

    7

    8

    TAAGT___

    3

    2

    ______CC

    4

    3

    3

    7

    7

    1

    __AGA___

    4

    2

    ___GA-C_

    Ref

    TCAGAACC

    The positions, reads, and relationships can be shown explicitly as a -matrix (see Relation Vectors for an explanation of the relationships):

    +matrix (see Relation Vectors for information on the relationship codes):

    @@ -279,7 +291,7 @@

    Relate batch: Example

    1

    - + @@ -304,7 +316,7 @@

    Relate batch: Example

    - + @@ -332,17 +344,17 @@

    Relate batch: Example

    end3s: [8, 6, 7, 8, 7]

  • muts:

    {1: {},
    - 2: {64: [3]},
    + 2: {16: [3]},
      3: {128: [1]},
      4: {},
      5: {3: [4], 128: [1, 3]},
      6: {3: [4]},
    - 7: {16: [0]},
    - 8: {}
    + 7: {64: [0]},
    + 8: {}}
     

    Note that the numbers are shown here for visual simplicity as list -objects, but would really by numpy.ndarray objects.

    +objects, but would really be numpy.ndarray objects.

  • @@ -353,7 +365,7 @@

    Relate batch: Example

    255

    1

    16

    64

    1

    1

    3

    1

    64

    16

    1

    1

    128

    ++++ + + + + + + + + + + + + + + + + + + + +

    Base

    2-Bit Binary Code

    A

    00

    C

    01

    G

    10

    T/U

    11

    +

    For efficient storage of (long) nucleic acid sequences, the following +algorithm compresses 4 bases (= 8 bits/byte ÷ 2 bits/nt) in each byte, +and then restores the original sequence when needed.

    +
    +
    +

    Algorithm for Sequence Compression

    +
    +

    Algorithm for Sequence Compression: Procedure

    +

    First, attributes of the nucleic acid sequence are recorded:

    +
      +
    • rna (bool): whether the sequence is RNA

    • +
    • length (int): number of bases in the sequence

    • +
    • ns (tuple[int, ...]): positions of N bases, if any

    • +
    +

    The type and the length are scalar values with constant sizes regardless +of the length of the sequence. +The 0-indexed positions of ambiguous bases is an array that at worst (if +all bases are N) scales linearly with the length of the sequence and +at best (if no bases are N) is a constant (small) size. +Because most reference sequences contain no or very few bases that are +N, recording the N positions generally requires little space.

    +

    Then, each non-overlapping segment of four bases is encoded as one byte +by concatenating the 2-bit codes (above) of the bases in the segment, in +reverse order. +Ambiguous (N) bases are arbitrarily encoded as 00. +Because this step requires the length of the sequence to be a multiple +of 4, the sequence is padded on its 3’ side with A (an arbitrary +choice) until its length is a multiple of 4.

    +
    +
    +

    Algorithm for Sequence Compression: Example

    +

    Given the DNA sequence CAGNTTCGAN, the attributes are extracted:

    +
      +
    • rna: False

    • +
    • length: 10

    • +
    • ns: (3, 9) (note: these positions are 0-indexed)

    • +
    +

    The sequence is then padded with A at the 3’ end until its length +becomes a multiple of 4 (in this case, length 12):

    +

    CAGNTTCGANAA

    +

    Then, each 4-base segment is transformed into one byte by encoding each +base and concatenating the codes in order from code 4 to code 1:

    + +++++++++++++ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +

    Number

    Sequence

    Base 1

    Base 2

    Base 3

    Base 4

    Code 4

    Code 3

    Code 2

    Code 1

    Byte

    1

    CAGN

    C

    A

    G

    N

    00

    10

    00

    01

    00100001

    2

    TTCG

    T

    T

    C

    G

    10

    01

    11

    11

    10011111

    3

    ANAA

    A

    N

    A

    A

    00

    00

    00

    00

    00000000

    +

    Thus, the compressed byte string is [00100001, 10011111, 00000000]. +Note that this string is only 3 bytes, compared to 10 for the original. +As a bytes object, the representation is b'!\x9f\x00'.

    +
    +
    +
    +

    Algorithm for Sequence Decompression

    +
    +

    Algorithm for Sequence Decompression: Procedure

    +

    Beginning with a compressed sequence of bytes, each byte is transformed +into four bases by decoding each 2-bit chunk to a base using the above +table, then reversing the order of the four bases. +The code 11 is decoded to U if the rna attribute is True +and to T if False. +The sequence at this point must have a number of bases divisible by 4. +It is cut to the correct number of bases using the length attribute. +Finally, every position in the attribute ns is masked to N.

    +
    +
    +

    Algorithm for Sequence Decompression: Example

    +

    Suppose that a compressed sequence has the following attributes:

    +
      +
    • compressed byte string: [00100001, 10011111, 00000000]

    • +
    • rna: False

    • +
    • length: 10

    • +
    • ns: (3, 9)

    • +
    +

    To decompress the sequence, each byte is split into four 2-bit segments, +decoded, reversed, and reassembled:

    + +++++++++++++ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +

    Number

    Byte

    Code 1

    Code 2

    Code 3

    Code 4

    Base 4

    Base 3

    Base 2

    Base 1

    Sequence

    1

    00100001

    00

    10

    00

    01

    C

    A

    G

    A

    CAGA

    2

    10011111

    10

    01

    11

    11

    T

    T

    C

    G

    TTCG

    3

    00000000

    00

    00

    00

    00

    A

    A

    A

    A

    AAAA

    +

    The resulting sequence, CAGATTCGAAAA, is trimmed to 10 nt: CAGATTCGAA. +Finally, (0-indexed) positions 3 and 9 are replaced with N: CAGNTTCGAN.

    +
    +
    +
    + + + + + + + +
    + + + + + \ No newline at end of file diff --git a/docs/algos/index.html b/docs/algos/index.html index 143fb08a..3c109ebf 100644 --- a/docs/algos/index.html +++ b/docs/algos/index.html @@ -19,7 +19,7 @@ - + @@ -52,7 +52,10 @@
  • seismicrna package
  • File Formats
  • Data Structures
  • -
  • Algorithms
  • +
  • Algorithms +
  • Bugs and Requests
  • How to Write this Manual
  • @@ -83,6 +86,16 @@

    Algorithms

    +
    @@ -90,7 +103,7 @@

    Algorithms - +
    diff --git a/docs/data/relate/refseq.html b/docs/data/relate/refseq.html index 42b46670..65f45b0a 100644 --- a/docs/data/relate/refseq.html +++ b/docs/data/relate/refseq.html @@ -103,39 +103,8 @@

    Reference Sequence

    A RefseqIO object stores a reference sequence as a space-efficient CompressedSeq object in the private attribute _s. -The original sequence is available via the cached property refseq.

    -

    In a standard sequence, each base is represented by one character, and -hence occupies 1 byte (8 bits). -But because each base has only 4 possibilities, each base requires only -log2(4) = 2 bits, shown in the following table:

    - ---- - - - - - - - - - - - - - - - - - - - -

    Base

    Binary Code

    A

    00

    C

    01

    G

    10

    T

    11

    -

    A CompressedSeq object uses this code to compress 4 bases -(= 8 bits/byte ÷ 2 bits/nt) into each byte. -Each has the following attributes:

    +(The original sequence is available via the cached property refseq.) +A CompressedSeq object has the following attributes:

    -
  • Algorithms
  • +
  • Algorithms +
  • Bugs and Requests diff --git a/docs/issues.html b/docs/issues.html index 843f8b04..b7491736 100644 --- a/docs/issues.html +++ b/docs/issues.html @@ -20,7 +20,7 @@ - + @@ -142,7 +142,7 @@

    Guidelines for requesting new features and other enhancements - + diff --git a/docs/objects.inv b/docs/objects.inv index fbc25427710cf0a145367addec30c933a4e20f04..c682bb08b967fc2ba65894c8e6628e18da1b32e2 100644 GIT binary patch delta 11228 zcmV<2DC@InSx0(7kJS@5PttSMqG5o;A;H*&ODKkf*wk?S_ld%9k%$P4fF zAlNQZcB?N|*ZkQD2GdSYM0QnG5VW)+Rbc~!r@dPCKQ4ee|L5k z3L|1X7!PwZ`dyeOUC5LU|#GWvr}PD|7t5z z##vAea@TzB^7%$1b5&!%_bQp#o}|w#_TvTnm*lJUdd2Ple{#vHM(m9UzGh)%E?o)c z|NYMym#5GQuiNM%GC>A*ozTlA{f@RK=FkB#N9R|M?Yw z+7cj69vO%jd*Wg+oISRU1*Z6lh)gwaM9MqbvI(Q8I@;2oys%s9EE&L`(&vC648@T@ zaxomz9&yDAQ_N*_rrS6oBb@Eo?8u2w-(bm$IJ4zLf1K&_0A3awh+CXpm_yj(DO62y z1hdE6Ga{X>`c4hk=2+R1_gLwZmxdgQ4h?1-*|eTBi42b%S`S^=LfccdWsNCqo2bpR zc0_KEvTL)?X@Ai+*bzZ4%=zkM*04e2OU>A1u_Opf3YS!H9CWl~U56bICOufI0)0eZ z3n1)Jf2u)1r^;48Xw-2i!1YrX*6{YEY+Gc?NF{=EO&(FYM%uPH-RETIRPAV(i-Amb zGtFYulu|T0d29*8lEf)B#?-%Bc}xYQW7jZADAZC%34#|V4M?e zOpR~bF{aw*_8Om?Dtm58(%iL*=fkx-cd87zu}_N> zrR?RJx|)xvrn#ji)=>ZdNO;q8grw#byk 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delta 270 zcmeC!!?k)3S3?Wq6sD<+eOsq78Hq49Z$Bx*6vjCHhdYx9TXKF%YH`A3hY1psFZ8f) z4;Eu`VUd=$O36>QO3>5OPf09E)GtcSNi0d#2P>VvR*FeRR0ONkO(`au=@&jQN>104 zX7ZTc@57{sUFG)W(o6;Rj31`+#xNO6CL}oL=N1&D78j?c1g91zByi;?Wu+#UC~S|9 tX0m705X4Y0C4)V7N`_buFN((Ldt#WpIbfQn^T#qtzEncoding ambiguous relationships -

    Low-quality base calls

    +
    +

    Encoding low-quality base calls

    A low-quality base call is defined as having a Phred quality score below the user-specified threshold (default: 25). Low-quality base calls are processed as if they could be any of the four nucleotides. The type @@ -342,8 +342,8 @@

    Low-quality base calls

    -
    -

    Ambiguous insertions and deletions

    +
    +

    Encoding ambiguous insertions and deletions

    Insertions and deletions (collectively, “indels”) in the read can cause ambiguities that even the highest quality sequencing reads could not prevent. When one or more bases are inserted or deleted in a repetitive @@ -434,8 +434,8 @@

    Encoding paired-end reads -

    Consensus relationships

    +
    +

    Encoding consensus relationships

    When finding the consensus of two mates, information in one mate should compensate for a lack thereof in the other. Recall that a relationship byte indicates all possible relationships at its position. The more bits @@ -503,8 +503,8 @@

    Consensus relationships

    -
    -

    Irreconcilable relationships

    +
    +

    Encoding irreconcilable relationships

    It is possible, although rare, for mates 1 and 2 to share no bits. For example, if mate 1 were a high-quality match (00000001) and mate 2 were a high-quality substitution to T (10000000), then the bitwise diff --git a/docs/manuals/workflow.html b/docs/manuals/workflow.html index 8269aed9..f4e6bec6 100644 --- a/docs/manuals/workflow.html +++ b/docs/manuals/workflow.html @@ -733,7 +733,7 @@

    Relate setting: Minimum Phred scoreT is called as a match with a Phred score of 20, then it would be marked as possibly a match and possibly a subsitution to A, C, or G. -See Relation Vectors for more information.

    +See Encoding low-quality base calls for more information.

    Relate setting: Ambiguous insertions and deletions

    @@ -793,8 +793,8 @@

    Relate output file: Batch of read names

    Relate output file: Reference sequence

    -

    The relate step writes the reference sequence as a CompressedSeq -object to the file refseq.brickle. +

    The relate step writes the reference sequence as a RefseqIO object +to the file refseq.brickle. See Reference Sequence for details on the data structure. See Brickle: Compressed Python Objects for details on brickle files.

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8,61,73],convent:11,converg:[10,44,45],convers:[69,76],convert:[8,9,11,15,18,22,25,31,38,54,71],convert_path:15,coord:[25,31,33,44,45],coordin:[25,31,33,38,44,45,59,61],copi:[4,59,60,61,76],core:[7,8,10,29,30,31,32,33,34,35,36,38,39,42,44,74],corner:78,correct:[5,16,42,66,76],correctli:[54,66,76],correl:45,correl_method:32,correl_min_n:32,correspond:[8,25,34,38,47,51,54,66,76],corrupt:78,cost:76,costli:76,could:[11,25,35,47,53,54,69,73,76],count:[8,10,11,13,18,22,25,32,33,34,36,42,44,45,47],count_base_typ:13,count_cigar_mut:[35,36],count_del:[22,33,44],count_fastq_read:8,count_in:[22,33,44],count_per_po:13,count_per_read:13,count_ref:22,count_single_pair:18,count_sub:22,count_total_read:18,count_uniq_read:10,counterpart:76,counts_ob:42,counts_per_uniq:10,cours:69,cover:[13,36,42,48,79],cover_per_po:13,cover_per_read:13,coverag:[22,36,76],coverage_matrix:13,cpu:70,cram:[8,18,34,44,45,53,55,57,62,76],crash:[20,76],creat:[10,11,20,26,34,36,45,54,67,70,76,78],create_path_typ:11,create_report:[28,33],create_temp_sam:34,creation:69,credit:80,criterion:10,critic:[11,74],cs_type:32,csh:68,csv:[10,25,29,31,32,33,42,44,45,58,59,60,61,76],ct2dot:[31,66],ct:[24,31],ct_data:24,ct_file:[24,31],ct_path:24,ct_text:24,ctg:47,ctrl:74,curl:79,current:[10,16,34,38,66,69,70,76],curv:24,cut:[5,8,44,45,54,76],cut_:[8,44,45],cut_a1:[8,44,45],cut_a2:[8,44,45],cut_discard_trim:[8,44],cut_discard_untrim:[8,44],cut_g1:[8,44,45],cut_g2:[8,44,45],cut_indel:[8,44],cut_m:[8,44,45],cut_nextseq:[8,44],cut_o:[8,44,45],cut_q1:[8,44,45],cut_q2:[8,44,45],cutadapt:[8,44,45,54,62],cutadapt_cmd:8,cutadapt_input_arg:8,cv1:69,cv2:69,cyan:11,cytidin:1,d1:47,d:[11,22,32,36,45,68],data1:[10,11,32,33],data2:[10,11,32,33],data:[10,11,13,15,16,24,32,33,34,42,45,49,51,52,57,59,60,62,63,64,65,74,75,76,79],data_sect:24,databas:71,datafram:[8,10,13,16,24,29,32,42],dataset:[10,11,16,32,33,34,42,45,65,75,76,77,79],datasetload:11,date:[60,74],datefmt:11,datetim:[10,11,33],db:24,db_path:24,dcm:74,dd:74,dd_hh:74,de:0,deactiv:[66,67,68],debug:[11,43,69,74],dec:47,decim:[11,47],decod:[5,18],decode_phr:18,decompil:22,decompress:[6,25,50,65,76],decor:21,decreas:10,dedupl:11,deem:21,deepli:76,default_report_field:11,defeat:76,defin:[7,10,11,12,25,34,38,43,45,47,51,59,60,61],definit:[25,76],degener:25,del:[38,45],del_pos3:38,del_pos5:38,delet:[8,9,22,33,34,36,38,44,45,69,71,78],delete_fastq:28,delete_fq:28,delete_temp_sam:34,delete_text_fastq:28,deliber:52,delta_log_lik:10,demult:[7,65],demult_on:44,demulti:45,demulti_overwrit:[28,44,45],demulti_workspac:28,demultiplex:[8,28,44,45,53,54,62],demultiplex_run:28,demultiplex_workspac:28,denot:[8,38,51],dens:52,depdend:66,depend:[11,14,15,20,67,68,69,75,76],dependency_exist:14,deprec:69,depth:[73,76],deriv:[23,25],describ:[9,16,68,72],descript:[12,51,59,60,61,70,76],desigin:[44,45],design:[44,45],desir:68,destin:[8,30,31,34,44,45],destroy_temp_data:28,detail:[4,10,11,16,50,54,70,71,72,74,76],detect:76,determin:[1,8,11,15,18,20,21,25,38,47,53,76],develop:[0,1,69,74],deviat:70,diagram:54,dict:[8,10,11,12,13,15,18,20,22,24,25,26,28,29,32,34,35,36,38,42,51,62,64],dict_to_pair:24,dictionari:[24,28],did:[70,76],differ:[16,45,47,51,54,66,69,74,76,77,78],difficult:47,difficulti:56,digest:15,digest_data:15,digit:47,dimension:[10,17,51],dimethyl:1,dir:[45,71,76],dir_field:24,dir_seg:24,dir_seg_typ:[8,15],direct:38,directli:[11,16,32,53,54],directori:[8,9,11,15,21,24,32,42,54,56,71,73,76,78],directoy_to_write_to:28,disabl:[10,33,44,45,74,76],discard:[8,44,45,62],discord:[8,44,45,62,76],discordantli:76,discount:[22,33,45],discount_mut:[33,44,45],discourag:11,discrep:76,discuss:70,dispatch:20,distanc:[8,44,45,62],distinct:76,distinguish:76,distribut:[4,11,69],divid:75,divis:5,dm:[3,16,24,60,65,76],dmfastqi:[8,44,45,76],dmfastqx:[8,44,45,76],dmfastqz:[8,44,45,76],dms_file:24,dna:[5,8,11,13,15,25,26,31,32,33,34,35,36,38,39,44,50,53,71,76],doc:68,docdef:12,docstr:[9,12,20],document:[12,38,45,68,76,80],doe:[11,21,24,36,38,44,45,47,68,69,70,76,78],doi:16,dot2ct:31,dot:[24,31],dot_fil:[24,31],doubl:[24,47,66,74,76],dovetail:[8,44,45,62,76],downgrad:69,download:[68,69,79],downstream:[51,61],dozen:69,dpad:45,dreem:[3,29,65],drop:[12,16,54],drop_close_mut:17,drop_fail:20,dtime:11,dtype:11,due:[38,76],duplic:[10,12,26,70,76],durat:60,dure:[8,38,44,45,52,54,60,62,66,74,76,78],e1:47,e2:[45,76],e:[10,11,16,25,35,45,53,54,69,71,73,76],each:[5,8,9,10,11,12,13,16,17,18,20,22,24,25,26,28,29,31,32,33,34,35,36,38,42,44,45,47,49,50,51,52,53,54,56,58,61,66,69,72,74,78],easi:[8,16,63],easier:[68,74],easiest:[68,73],edit:[8,9,56],edu:[31,66],effect:68,effici:[5,38,50,76],effort:[56,67,76],eight:47,either:[4,22,25,36,42,47,54,68,76],element:[10,35,38],els:[11,25,76],elsewher:76,em:[10,45],em_clust:10,em_run:[10,44,45],em_thresh:[10,44,45],em_typ:32,emclust:10,emit:76,empti:[17,25,26],en:[10,68],enabl:[44,45,76],enc:[45,76],encapsul:25,encod:[5,8,11,18,34,38,44,45,48,50,51,53,62,71],encode_compar:38,encode_match:[38,39],encode_phr:18,encode_rel:[38,39],encode_test:39,encount:[66,68],end31:38,end32:38,end3:[11,13,15,18,24,25,34,35,36,42,51],end51:38,end52:38,end5:[8,11,13,15,18,24,25,34,35,36,39,42,51],end:[5,8,10,11,12,13,18,24,25,26,31,33,34,35,36,44,45,48,56,61,62,64,80],energi:31,ensembl:[42,45],ensur:[8,38,53,76],ensure_ord:13,ensure_same_length:13,entail:70,enter:[25,66,69],entir:[25,44,47,54,72,74,75],entireti:76,entri:[7,18,43],entropi:10,env:[66,68],environ:[65,67],equal:[8,17,26,35,47,76],equival:53,eras:[38,45],erron:76,error:[8,11,14,17,25,36,38,44,45,53,62,66,68,70,74],es:11,es_typ:32,esac:68,escap:11,especi:[8,66,68,70,76],essenti:[8,76],estim:[10,16],et:[1,16],etc:68,eukaryot:76,even:[4,8,10,11,22,29,30,31,32,33,34,42,44,45,47,76],event:74,everi:[5,8,10,11,17,20,22,24,25,26,32,34,35,38,39,42,45,47,51,53,63,66,69,71,72,73,79],evid:80,exact:[47,70],exactli:[11,25,61,62,66,76],examin:76,exampl:[8,9,11,22,45,47,48,54,56,58,63,65,68,69,70,73,74,76,77,79],excel:[58,69],except:[11,20,22,26,36,38,63,71,74,76],exception:16,exclud:[15,16,45],exclude_def:12,exclude_default:12,exclude_gu:[33,44],exclude_po:[33,44,45],exclude_polya:[33,44,45],execut:[34,68],exist:[8,10,11,14,16,21,29,30,31,32,33,34,38,42,44,45,53,69,70,71,76],exit:[8,31,34,44,45],expand_degenerate_seq:[25,26],expect:[8,10,16,25,36,45,70],expedit:69,explain:72,explan:[45,76],explicit:32,explicitli:[25,38,51,52,73],export_cmd:8,export_sampl:29,expos:[7,8],express:47,ext:[11,32,42],ext_typ:11,extant:[8,38],extend:[25,71],extens:[8,11,42,44,45,62,76],extern:[7,11],extra:[12,34,47,66,76],extra_default:12,extra_docstr:12,extract:[5,8,18,25,45,76],extract_fasta_seqnam:25,extran:76,extrem:76,f9874e5c37b56316d47bacc0a7cc604:64,f:[11,54,74,76],f_ob:16,f_rtol:16,f_tol:16,fa:[53,71,76,78],factori:[44,45],faidx:[18,76],fail:[8,20,26,44,45,62,66,74,76],failur:[20,74],fall:[16,76],fals:[5,8,10,11,13,15,17,18,20,21,22,24,25,28,29,30,31,32,33,34,38,42,44,50,62,76],falsi:26,familiar:[66,69],fanci:22,fashion:32,fasta:[7,8,18,24,25,28,31,34,44,45,55,57,65,72,76,78],fasta_path:28,fasta_test:26,fastaclean:[7,71],faster:76,fastq1:8,fastq1_path:28,fastq2:8,fastq2_path:28,fastq:[8,9,18,28,34,44,45,53,55,56,57,62,66,73,78],fastq_arg:8,fastq_fil:8,fastq_gz:8,fastq_id:28,fastq_nam:28,fastq_to_dict:28,fastq_to_search:28,fastq_to_writ:28,fastqc:[8,9,44,45,62,66],fastqc_cmd:8,fastqi:[8,44,45,76],fastqunit:[8,28],fastqx:[8,28,44,45,73,76],fastqz:[8,44,45,76],fatal:74,favor:[8,9],feat:69,featur:73,feedback:65,fetch:42,fetch_count:42,fetch_ratio:42,few:[5,16,33,76],fewer:[16,17,76],ff:[45,47,54,76],ffff:54,fffff:54,fffffff:54,fffffffff:54,field:[8,10,11,15,32,33,34,38,42,58,63,76],field_kei:11,field_nam:11,field_titl:11,field_typ:11,field_valu:11,figur:[32,47],figure_align:8,file:[7,9,10,11,15,18,24,25,28,29,30,31,32,33,34,38,42,44,45,49,55,58,59,60,61,62,63,64,65,70,72,79],file_seg_typ:[8,10,15,33,34],fileexistserror:11,fileio:[11,15],filenotfounderror:11,fill:[11,16],fill_whitespac:11,filter:[10,17,18,21,28,33,45,62],find:[8,10,11,13,16,35,36,38,44,45,47,76],find_ambrel:38,find_best_ord:10,find_cigar_op_po:[35,36],find_fil:11,find_files_chain:11,find_op:35,find_rels_lin:38,find_rels_read:38,find_root_pair:24,find_tabl:42,finds_multigrepped_read:28,finfo:[33,45],finish:[8,30,34,44,45,66,69,72,76],first:[5,10,11,14,16,25,32,38,47,51,52,53,59,60,61,66,71,76],fish:68,fit:[4,11,13,22,53],fit_uint_s:11,fit_uint_typ:11,five:[49,51,72],fix:70,fl:52,flag:[8,11,18,38,74,76],flags_exc:18,flags_req:18,flagstat:[18,34],flagstat_cmd:18,flank:[9,38],fmt:11,fmt_fanci:22,fmt_func_arg:20,fmt_plain:22,fmut:[33,45],fna:53,focu:69,fold:[7,44,53,61,65,76],fold_profil:31,fold_rna:31,follow:[0,5,8,22,25,35,45,47,50,51,52,53,54,56,66,68,69,70,71,72,76,78],forbid:26,forc:[8,10,11,29,30,31,32,33,34,42,44,45,71,74],foreign:76,forg:66,form:[13,16,22,47,76],format:[8,11,18,20,22,25,38,44,45,65,71],format_clust_nam:11,format_exp_count_col:10,format_fasta_name_lin:[25,26],format_fasta_record:[25,26],format_fasta_seq_lin:25,format_metadata:29,format_seri:29,format_vers:11,formatt:11,former:76,formula:10,fortun:69,forward:[25,31,33,44,45,61,76],found:[8,10,16,44,45,62,66,68,70],foundat:4,four:[5,25,36,47,54,66,71],fpath:28,fpr:24,fq1:28,fq2:28,fq:[9,54,76,78],fq_inp:8,fq_name:76,fq_out:[8,18],fq_pipelin:8,fq_unal:8,fq_unit:[8,28],fqop:8,fqs_pipelin:8,fqunit:[8,28],fqutil:9,fqutil_test:9,fr:[8,44,45,62,76],fraction:[10,16,17,33,44,45],fragil:76,fragment:[8,44,45,62],frame:[8,10,13,16,29,32,42],frame_test:17,framework:74,free:[4,31],freeli:[45,72],frmt:11,from3to5:38,from:[5,7,8,9,10,11,12,15,16,18,20,24,25,26,31,32,34,35,36,38,39,42,44,45,47,53,54,62,69,70,71,72,74,79,80],from_clust:10,from_count:22,from_ct:24,from_dataset:10,from_dict:11,from_path:8,from_read:34,from_report_format:22,front:28,frustrat:69,fse:74,fse_r1:78,fse_r2:78,full:[10,11,25,26,38,73,76],fulli:[38,47],fulltabul:42,func:[12,20],further:1,futur:[54,69,80],fwd:25,fwd_clip:28,fwd_primer:28,g1:[45,76],g2:[45,76],g:[5,8,9,22,25,32,33,36,44,45,47,53,54,69,76],gap:[8,16,26,31,33,44,45,51,61,76],gap_extend:[8,9],gap_open:[8,9],gb:54,gbar:45,gcatgctagcca:54,gctcttccgatct:[8,44,62,76],gencod:71,gener:[4,5,8,9,10,11,16,22,25,26,32,34,39,72,76],generate_batch:34,get:[8,9,10,11,12,18,21,24,25,32,34,35,42,68],get_alphaset:25,get_args_count_fastq_read:8,get_batch_path:11,get_batch_seg:[10,33],get_batch_typ:11,get_bowtie2_index_path:8,get_btype_nam:11,get_byte_dtyp:11,get_cmap:32,get_cmap_typ:32,get_colormap:32,get_common_best_run_attr:10,get_common_ord:10,get_comp:25,get_comptran:25,get_coords_by_ref:25,get_count_path:10,get_count_per_po:13,get_count_per_read:13,get_cover_per_po:13,get_cover_per_read:13,get_coverage_matrix:13,get_data_typ:[10,11,33,34],get_dataset1_typ:[10,11,33],get_dataset2_typ:[10,11,33],get_db_struct:29,get_dir:24,get_docstr_lin:12,get_dtyp:11,get_fasta:24,get_fasta_seq:25,get_field:11,get_fil:24,get_flagstat:18,get_half_coverage_matrix:13,get_info:13,get_invers:13,get_length:13,get_lock:21,get_log_exp_obs_count:10,get_mask:25,get_matrix:10,get_max_uint:11,get_max_valu:11,get_mu:10,get_non_seq_regex:30,get_nonalphaset:25,get_null_valu:42,get_num_parallel:20,get_oconv_array_float:11,get_oconv_dict_float:11,get_oconv_dict_list_float:11,get_oconv_float:11,get_oconv_list_float:11,get_other_iupac:25,get_param_default:12,get_param_lin:12,get_path:[15,32],get_path_field:32,get_path_seg:32,get_pictran:25,get_prop:10,get_quantil:[16,17],get_reads_per_batch:34,get_reads_per_po:13,get_ref_metadata:29,get_refseq_typ:11,get_rel_nam:42,get_rels_per_po:13,get_rels_per_read:13,get_report_typ:[10,11,33,34],get_resp:10,get_sample_data:29,get_sample_metadata:29,get_sample_ref_ext:8,get_sect_coords_prim:25,get_sect_metadata:29,get_seq_base_bar_trac:32,get_seq_base_scatter_trac:32,get_seq_line_trac:32,get_seq_stack_bar_trac:32,get_table1_typ:32,get_table2_typ:32,get_table_data:29,get_table_param:32,get_table_path:10,get_table_typ:32,get_tabulator_writ:42,get_tabulator_writer_typ:42,get_top_logg:11,get_trac:32,get_uint_dtyp:11,get_uint_s:11,get_uint_typ:11,get_uniq_nam:10,get_uniq_read:10,get_verbos:11,github:[0,38,70,79],give:[11,12,15,17,61,65,66,69,73,76],given:[5,8,10,11,12,14,15,16,18,20,22,25,26,32,34,35,38,39,42,47,51,58,59,60,73,74,76,78],glob:73,global:74,gnu:4,go:[24,44,45,76],goe:[35,69],good:[47,78],googl:0,gpu:70,graph:[7,65],graph_filenam:32,graph_obj:32,graph_typ:32,graphbas:32,graphwrit:32,greater:[8,9,16,26,76],green:11,grep:[28,44,45],grep_both_fastq:28,grote:0,group:[25,76],gs:[8,44,45,62,76],gt_eq:13,gu:[25,45],guess:16,guid:[45,54,68,76,79],guidanc:66,guidelin:80,gz:[42,54,76,78],gzip:[8,42,54],ha:[5,9,11,16,17,25,34,35,36,38,47,50,52,53,54,66,69,70,74,76],had:[16,66,70,76],halfrefpattern:22,halfrelpattern:22,halt:74,handl:[71,76],handler:11,happen:[52,74],hard:76,harder:38,harvard:0,has_close_mut:17,has_ipath:14,has_opath:14,hash:25,hashabl:[26,32],have:[4,5,9,11,16,25,33,38,47,51,54,66,68,69,70,71,73,76],head:76,header:[8,11,18,24,42,47,54,71,76],header_depth:42,header_row:42,header_test:27,header_typ:42,helix:24,help:[16,45,76],helper:42,henc:[5,47,76],here:51,hex:47,hexadecim:[15,47],hh:74,hi_qual:35,hide:69,high:[8,16,35,44,45,47,62,76],higher:74,highest:47,highli:69,histori:38,home:[66,68],hook:68,hope:4,hopefulli:80,horizont:66,how:[20,24,47,65,67,68,71,72],howev:[47,51,73,76],ht:38,html:[31,32,45,66,68,76],htslib:66,http:[4,10,16,31,38,66,68,78,80],hui:60,human:[35,58],humanize_relvec:35,hundr:76,hybrid:20,hyphen:[25,26],hyphenate_end:25,i:[10,11,16,20,22,25,32,35,36,45,49,51,53,54,56,71,73,76],iconv:11,iconv_array_float:11,iconv_array_int:11,iconv_datetim:11,iconv_dict_str_int:11,iconv_int_kei:11,idea:[69,78],ideal:[11,47],ident:[10,26,47],identifi:[38,76],idxstats_cmd:18,ifasta:30,ignor:[25,26,33,44,45,53,74],iii:76,illeg:[26,53,71],illumina:54,illustr:[47,54],imag:80,immedi:[47,74],implement:69,impli:4,implicitli:[38,73],impos:53,imposs:47,improperli:76,improv:[11,70,76],inadvert:78,includ:[10,16,25,45,53,58,61,66,70],inclus:61,incompat:69,inconsist:[11,53],inconveni:76,incorpor:53,incorrect:[54,76],incorrectli:54,increas:[44,45,76],increment:51,incur:76,indel:[8,9,34,38,44,45,47,62,76],independ:[10,16,44,45],index:[5,8,10,11,13,16,18,25,26,28,31,32,33,34,35,38,42,44,45,49,50,51,53,61,66],index_clust:11,index_col:42,index_depth:42,index_fasta_cmd:18,index_ord:11,index_order_clust:11,index_orders_clust:11,index_pfx:8,index_to_po:[25,26],index_to_seq:[25,26],index_toler:[28,44,45],index_xam_cmd:18,indic:[20,38,47,54,71,76],indiv:45,individu:[0,8,32,44,45,62,66,73,76],inequ:[8,9],infer:[12,35,42,59,60],infer_read:[35,36],infer_report_loader_typ:42,infer_test:36,info:[43,74],inform:[10,11,33,38,39,44,45,47,49,51,52,53,54,56,58,70,73,76],infrequ:38,inher:76,init:[33,68,76],initi:[8,16,38,43,44,45,53,62,67,71,76],inn:38,input:[8,14,65,70,71,72],input_csv:28,input_path:[10,29,31,32,33,34,42,44,45],ins:45,ins_len:35,ins_po:38,insensit:22,insert:[8,9,22,33,34,35,36,38,39,44,45],insid:17,inspect:12,instal:[65,69],instanc:[10,11,22,25,26,36],instanti:[11,26],instead:[11,66,69,76],instruct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39,encount:[66,68],end31:38,end32:38,end3:[11,13,15,18,24,25,34,35,36,42,51],end51:38,end52:38,end5:[8,11,13,15,18,24,25,34,35,36,39,42,51],end:[5,8,10,11,12,13,18,24,25,26,31,33,34,35,36,44,45,48,56,61,62,64,80],energi:31,ensembl:[42,45],ensur:[8,38,53,76],ensure_ord:13,ensure_same_length:13,entail:70,enter:[25,66,69],entir:[25,44,47,54,72,74,75],entireti:76,entri:[7,18,43],entropi:10,env:[66,68],environ:[65,67],equal:[8,17,26,35,47,76],equival:53,eras:[38,45],erron:76,error:[8,11,14,17,25,36,38,44,45,53,62,66,68,70,74],es:11,es_typ:32,esac:68,escap:11,especi:[8,66,68,70,76],essenti:[8,76],estim:[10,16],et:[1,16],etc:68,eukaryot:76,even:[4,8,10,11,22,29,30,31,32,33,34,42,44,45,47,76],event:74,everi:[5,8,10,11,17,20,22,24,25,26,32,34,35,38,39,42,45,47,51,53,63,66,69,71,72,73,79],evid:80,exact:[47,70],exactli:[11,25,61,62,66,76],examin:76,exampl:[8,9,11,22,45,47,48,54,56,58,63,65,68,69,70,73,74,76,77,79],excel:[58,69],except:[11,20,22,26,36,38,63,71,74,76],exception:16,exclud:[15,16,45],exclude_def:12,exclude_default:12,exclude_gu:[33,44],exclude_po:[33,44,45],exclude_polya:[33,44,45],execut:[34,68],exist:[8,10,11,14,16,21,29,30,31,32,33,34,38,42,44,45,53,69,70,71,76],exit:[8,31,34,44,45],expand_degenerate_seq:[25,26],expect:[8,10,16,25,36,45,70],expedit:69,explain:72,explan:[45,76],explicit:32,explicitli:[25,38,51,52,73],export_cmd:8,export_sampl:29,expos:[7,8],express:47,ext:[11,32,42],ext_typ:11,extant:[8,38],extend:[25,71],extens:[8,11,42,44,45,62,76],extern:[7,11],extra:[12,34,47,66,76],extra_default:12,extra_docstr:12,extract:[5,8,18,25,45,76],extract_fasta_seqnam:25,extran:76,extrem:76,f9874e5c37b56316d47bacc0a7cc604:64,f:[11,54,74,76],f_ob:16,f_rtol:16,f_tol:16,fa:[53,71,76,78],factori:[44,45],faidx:[18,76],fail:[8,20,26,44,45,62,66,74,76],failur:[20,74],fall:[16,76],fals:[5,8,10,11,13,15,17,18,20,21,22,24,25,28,29,30,31,32,33,34,38,42,44,50,62,76],falsi:26,familiar:[66,69],fanci:22,fashion:32,fasta:[7,8,18,24,25,28,31,34,44,45,55,57,65,72,76,78],fasta_path:28,fasta_test:26,fastaclean:[7,71],faster:76,fastq1:8,fastq1_path:28,fastq2:8,fastq2_path:28,fastq:[8,9,18,28,34,44,45,53,55,56,57,62,66,73,78],fastq_arg:8,fastq_fil:8,fastq_gz:8,fastq_id:28,fastq_nam:28,fastq_to_dict:28,fastq_to_search:28,fastq_to_writ:28,fastqc:[8,9,44,45,62,66],fastqc_cmd:8,fastqi:[8,44,45,76],fastqunit:[8,28],fastqx:[8,28,44,45,73,76],fastqz:[8,44,45,76],fatal:74,favor:[8,9],feat:69,featur:73,feedback:65,fetch:42,fetch_count:42,fetch_ratio:42,few:[5,16,33,76],fewer:[16,17,76],ff:[45,47,54,76],ffff:54,fffff:54,fffffff:54,fffffffff:54,field:[8,10,11,15,32,33,34,38,42,58,63,76],field_kei:11,field_nam:11,field_titl:11,field_typ:11,field_valu:11,figur:[32,47],figure_align:8,file:[7,9,10,11,15,18,24,25,28,29,30,31,32,33,34,38,42,44,45,49,55,58,59,60,61,62,63,64,65,70,72,79],file_seg_typ:[8,10,15,33,34],fileexistserror:11,fileio:[11,15],filenotfounderror:11,fill:[11,16],fill_whitespac:11,filter:[10,17,18,21,28,33,45,62],find:[8,10,11,13,16,35,36,38,44,45,47,76],find_ambrel:38,find_best_ord:10,find_cigar_op_po:[35,36],find_fil:11,find_files_chain:11,find_op:35,find_rels_lin:38,find_rels_read:38,find_root_pair:24,find_tabl:42,finds_multigrepped_read:28,finfo:[33,45],finish:[8,30,34,44,45,66,69,72,76],first:[5,10,11,14,16,25,32,38,47,51,52,53,59,60,61,66,71,76],fish:68,fit:[4,11,13,22,53],fit_uint_s:11,fit_uint_typ:11,five:[49,51,72],fix:70,fl:52,flag:[8,11,18,38,74,76],flags_exc:18,flags_req:18,flagstat:[18,34],flagstat_cmd:18,flank:[9,38],fmt:11,fmt_fanci:22,fmt_func_arg:20,fmt_plain:22,fmut:[33,45],fna:53,focu:69,fold:[7,44,53,61,65,76],fold_profil:31,fold_rna:31,follow:[0,5,8,22,25,35,45,47,50,51,52,53,54,56,66,68,69,70,71,72,76,78],forbid:26,forc:[8,10,11,29,30,31,32,33,34,42,44,45,71,74],foreign:76,forg:66,form:[13,16,22,47,76],format:[8,11,18,20,22,25,38,44,45,65,71],format_clust_nam:11,format_exp_count_col:10,format_fasta_name_lin:[25,26],format_fasta_record:[25,26],format_fasta_seq_lin:25,format_metadata:29,format_seri:29,format_vers:11,formatt:11,former:76,formula:10,fortun:69,forward:[25,31,33,44,45,61,76],found:[8,10,16,44,45,62,66,68,70],foundat:4,four:[5,25,36,47,54,66,71],fpath:28,fpr:24,fq1:28,fq2:28,fq:[9,54,76,78],fq_inp:8,fq_name:76,fq_out:[8,18],fq_pipelin:8,fq_unal:8,fq_unit:[8,28],fqop:8,fqs_pipelin:8,fqunit:[8,28],fqutil:9,fqutil_test:9,fr:[8,44,45,62,76],fraction:[10,16,17,33,44,45],fragil:76,fragment:[8,44,45,62],frame:[8,10,13,16,29,32,42],frame_test:17,framework:74,free:[4,31],freeli:[45,72],frmt:11,from3to5:38,from:[5,7,8,9,10,11,12,15,16,18,20,24,25,26,31,32,34,35,36,38,39,42,44,45,47,53,54,62,69,70,71,72,74,79,80],from_clust:10,from_count:22,from_ct:24,from_dataset:10,from_dict:11,from_path:8,from_read:34,from_report_format:22,front:28,frustrat:69,fse:74,fse_r1:78,fse_r2:78,full:[10,11,25,26,38,73,76],fulli:[38,47],fulltabul:42,func:[12,20],further:1,futur:[54,69,80],fwd:25,fwd_clip:28,fwd_primer:28,g1:[45,76],g2:[45,76],g:[5,8,9,22,25,32,33,36,44,45,47,53,54,69,76],gap:[8,16,26,31,33,44,45,51,61,76],gap_extend:[8,9],gap_open:[8,9],gb:54,gbar:45,gcatgctagcca:54,gctcttccgatct:[8,44,62,76],gencod:71,gener:[4,5,8,9,10,11,16,22,25,26,32,34,39,72,76],generate_batch:34,get:[8,9,10,11,12,18,21,24,25,32,34,35,42,68],get_alphaset:25,get_args_count_fastq_read:8,get_batch_path:11,get_batch_seg:[10,33],get_batch_typ:11,get_bowtie2_index_path:8,get_btype_nam:11,get_byte_dtyp:11,get_cmap:32,get_cmap_typ:32,get_colormap:32,get_common_best_run_attr:10,get_common_ord:10,get_comp:25,get_comptran:25,get_coords_by_ref:25,get_count_path:10,get_count_per_po:13,get_count_per_read:13,get_cover_per_po:13,get_cover_per_read:13,get_coverage_matrix:13,get_data_typ:[10,11,33,34],get_dataset1_typ:[10,11,33],get_dataset2_typ:[10,11,33],get_db_struct:29,get_dir:24,get_docstr_lin:12,get_dtyp:11,get_fasta:24,get_fasta_seq:25,get_field:11,get_fil:24,get_flagstat:18,get_half_coverage_matrix:13,get_info:13,get_invers:13,get_length:13,get_lock:21,get_log_exp_obs_count:10,get_mask:25,get_matrix:10,get_max_uint:11,get_max_valu:11,get_mu:10,get_non_seq_regex:30,get_nonalphaset:25,get_null_valu:42,get_num_parallel:20,get_oconv_array_float:11,get_oconv_dict_float:11,get_oconv_dict_list_float:11,get_oconv_float:11,get_oconv_list_float:11,get_other_iupac:25,get_param_default:12,get_param_lin:12,get_path:[15,32],get_path_field:32,get_path_seg:32,get_pictran:25,get_prop:10,get_quantil:[16,17],get_reads_per_batch:34,get_reads_per_po:13,get_ref_metadata:29,get_refseq_typ:11,get_rel_nam:42,get_rels_per_po:13,get_rels_per_read:13,get_report_typ:[10,11,33,34],get_resp:10,get_sample_data:29,get_sample_metadata:29,get_sample_ref_ext:8,get_sect_coords_prim:25,get_sect_metadata:29,get_seq_base_bar_trac:32,get_seq_base_scatter_trac:32,get_seq_line_trac:32,get_seq_stack_bar_trac:32,get_table1_typ:32,get_table2_typ:32,get_table_data:29,get_table_param:32,get_table_path:10,get_table_typ:32,get_tabulator_writ:42,get_tabulator_writer_typ:42,get_top_logg:11,get_trac:32,get_uint_dtyp:11,get_uint_s:11,get_uint_typ:11,get_uniq_nam:10,get_uniq_read:10,get_verbos:11,github:[0,38,70,79],give:[11,12,15,17,61,65,66,69,73,76],given:[5,8,10,11,12,14,15,16,18,20,22,25,26,32,34,35,38,39,42,47,51,58,59,60,73,74,76,78],glob:73,global:74,gnu:4,go:[24,44,45,76],goe:[35,69],good:[47,78],googl:0,gpu:70,graph:[7,65],graph_filenam:32,graph_obj:32,graph_typ:32,graphbas:32,graphwrit:32,greater:[8,9,16,26,76],green:11,grep:[28,44,45],grep_both_fastq:28,grote:0,group:[25,76],gs:[8,44,45,62,76],gt_eq:13,gu:[25,45],guess:16,guid:[45,54,68,76,79],guidanc:66,guidelin:80,gz:[42,54,76,78],gzip:[8,42,54],ha:[5,9,11,16,17,25,34,35,36,38,47,50,52,53,54,66,69,70,74,76],had:[16,66,70,76],halfrefpattern:22,halfrelpattern:22,halt:74,handl:[71,76],handler:11,happen:[52,74],hard:76,harder:38,harvard:0,has_close_mut:17,has_ipath:14,has_opath:14,hash:25,hashabl:[26,32],have:[4,5,9,11,16,25,33,38,47,51,54,66,68,69,70,71,73,76],head:76,header:[8,11,18,24,42,47,54,71,76],header_depth:42,header_row:42,header_test:27,header_typ:42,helix:24,help:[16,45,76],helper:42,henc:[5,47,76],here:51,hex:47,hexadecim:[15,47],hh:74,hi_qual:35,hide:69,high:[8,16,35,44,45,47,62,76],higher:74,highest:47,highli:69,histori:38,home:[66,68],hook:68,hope:4,hopefulli:80,horizont:66,how:[20,24,47,65,67,68,71,72],howev:[47,51,73,76],ht:38,html:[31,32,45,66,68,76],htslib:66,http:[4,10,16,31,38,66,68,78,80],hui:60,human:[35,58],humanize_relvec:35,hundr:76,hybrid:20,hyphen:[25,26],hyphenate_end:25,i:[10,11,16,20,22,25,32,35,36,45,49,51,53,54,56,71,73,76],iconv:11,iconv_array_float:11,iconv_array_int:11,iconv_datetim:11,iconv_dict_str_int:11,iconv_int_kei:11,idea:[69,78],ideal:[11,47],ident:[10,26,47],identifi:[38,76],idxstats_cmd:18,ifasta:30,ignor:[25,26,33,44,45,53,74],iii:76,illeg:[26,53,71],illumina:54,illustr:[47,54],imag:80,immedi:[47,74],implement:69,impli:4,implicitli:[38,73],impos:53,imposs:47,improperli:76,improv:[11,70,76],inadvert:78,includ:[10,16,25,45,53,58,61,66,70],inclus:61,incompat:69,inconsist:[11,53],inconveni:76,incorpor:53,incorrect:[54,76],incorrectli:54,increas:[44,45,76],increment:51,incur:76,indel:[8,9,34,38,44,45,47,62,76],independ:[10,16,44,45],index:[5,8,10,11,13,16,18,25,26,28,31,32,33,34,35,38,42,44,45,49,50,51,53,61,66],index_clust:11,index_col:42,index_depth:42,index_fasta_cmd:18,index_ord:11,index_order_clust:11,index_orders_clust:11,index_pfx:8,index_to_po:[25,26],index_to_seq:[25,26],index_toler:[28,44,45],index_xam_cmd:18,indic:[20,38,47,54,71,76],indiv:45,individu:[0,8,32,44,45,62,66,73,76],inequ:[8,9],infer:[12,35,42,59,60],infer_read:[35,36],infer_report_loader_typ:42,infer_test:36,info:[43,74],inform:[10,11,33,38,39,44,45,47,49,51,52,53,54,56,58,70,73,76],infrequ:38,inher:76,init:[33,68,76],initi:[8,16,38,43,44,45,53,62,67,71,76],inn:38,input:[8,14,65,70,71,72],input_csv:28,input_path:[10,29,31,32,33,34,42,44,45],ins:45,ins_len:35,ins_po:38,insensit:22,insert:[8,9,22,33,34,35,36,38,39,44,45],insid:17,inspect:12,instal:[65,69],instanc:[10,11,22,25,26,36],instanti:[11,26],instead:[11,66,69,76],instruct:[6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:[8,16,25,30,34,44,45,56,71,73,74,76],out_dir:[8,28,30,31,34,44,45],out_prefix:8,output:[8,10,14,17,18,29,30,31,32,33,34,36,42,44,45,62,63,70,71,72,73],output_func:10,outright:76,outsid:[17,47,76],over:[9,11,13,16,32,42],overal:1,overlap:[5,8,44,45,47,62,76],overwrit:[8,15,25,28,44,45,71],own:[10,32,45,66,69,74,76,77,79],p:[10,45,54],packag:[65,66,68,69],pad:[5,8,44,45,62],page:[70,78],pain:69,pair:[1,8,10,16,18,24,25,28,32,34,38,44,45,48,51,56,62],pair_dict:24,pairs_to_dict:24,pairs_to_t:24,panda:[8,10,11,13,16,24,25,29,32,42,44],parallel:[7,8,10,11,28,29,31,32,33,34,42,44,45,65,72],parallel_demultiplex:[28,44,45],parallel_grep:28,paralleliz:20,param:[8,12,32],param_default:12,param_doc:12,param_docstr:12,param_lin:12,param_list:12,paramdef:12,paramet:[8,10,11,12,16,18,20,22,25,29,30,31,32,33,34,35,38,42,43,44,45,63,72,76,79],parent:[8,11],pars:[8,9,11,14,15,25,29,31,39],parse_all_scor:9,parse_bowtie2:8,parse_cigar:[38,39],parse_ct:24,parse_db_str:24,parse_energi:31,parse_fasta:[25,26],parse_flagstat:18,parse_head:11,parse_idxstat:18,parse_path:15,parse_ref_head:18,parse_refs_metadata:29,parse_samples_metadata:29,parse_scor:9,parse_stdout_count_fastq_read:8,parse_vers:11,parsed_metadata:29,part:[11,24,25,26,53,61,74,76],partial:76,partialmutsbatch:[10,13,33],partialreadbatch:[10,13,33],partialtabul:42,particular:[4,35],particularli:73,partit:[10,76],pass:[11,20,52,73,76],pass_n_proc:20,patch:11,path:[8,10,11,14,15,18,21,24,25,29,30,31,32,33,34,42,44,55,68,71,73,76],path_field:[15,42],path_field_nam:15,path_seg:[10,33,42],patherror:11,pathlib:[8,10,11,15,18,21,24,25,29,30,31,32,33,34,42,44],pathtypeerror:11,pathvalueerror:11,pattern:[10,11,13,22,28,33,34,42,44,45,73],pattern_end:28,pattern_kei:33,pattern_start:28,pattern_typ:28,pcr:25,pd:[11,16,25],pdf:[32,45],penalti:[8,9],penultim:10,per:[10,11,18,25,28,32,34,42,44,45,54,76],per_read:13,percent:76,percentag:11,percentil:16,perform:[16,69,76],permit:[10,16,42,61],phenomena:47,phred:[8,18,34,38,44,45,47,56,62],phred_arg:8,phred_enc:[8,28,34,44,45],phred_encod:18,phred_scor:18,phred_test:19,physic:47,pickl:[15,28,52],pict:25,picto:25,pictogram:[25,26],piec:[11,54,69],pip:[65,66,67,69],pipelin:[7,8,14],place:[11,21,42,61],placehold:21,plain:[22,25],plan:69,pleas:[54,70,78],ploq:44,plot:45,plotli:32,plu:[7,68,71,73,76],pmut:44,pnext:8,po:[25,33,38,42,45,47],point:[5,7,10,38,43,76],poli:[25,33,44,45],polya:[25,45],pool:[10,20],popul:16,popular:69,portmanteau:52,pos3:13,pos5:13,pos_dtyp:13,pos_gu:33,pos_head:42,pos_index:13,pos_kept:33,pos_max_fmut:33,pos_min_ninfo:33,pos_num:[10,13,34],pos_polya:33,pos_us:33,posit:[5,8,10,13,14,16,20,24,25,26,30,32,33,34,35,36,38,42,43,44,45,48,50,72],possibl:[5,12,13,25,35,39,42,47,70,76],post:8,postabl:[29,32,42],postableload:[32,42],postablewrit:42,potenti:[14,53,76],power:54,pre:69,preced:38,precis:[11,29,42],predic:32,predict:[16,17,31,44,45,66,76],prefer:[9,10,15,78],prefix:[8,29],prerequisit:[65,66,67],preserv:[54,76],press:74,prevent:[8,47],previou:10,primari:[23,48,74],primarili:8,primer:[25,31,33,44,45,61,66,69],primer_gap:[25,31,33,44,45],primertupl:25,print:[43,45,66,68,74],print_multi_grep_dict:28,printabl:25,prior:38,prioriti:76,privat:50,probabl:[11,16,54,76],probe:[16,60,76],problem:[53,69,74,76],problemat:76,proc:45,procedur:16,process:[8,10,15,18,20,29,31,32,33,34,38,42,44,45,47,52,66,73,75,76,78,79],processor:[66,70],processs:79,produc:[11,17,47,53,63,76,78],product:[16,76],profil:[1,9,16,24,31,42,68,76],profilepost:42,program:[8,31,34,38,44,45,53,66,70,74],progress:74,proj1:76,proj2:76,project:[54,56,66,68,69,76],prone:[16,66],prop:10,prop_adj:10,prop_ob:[10,16],proper:32,properli:[66,68],properti:[8,10,11,13,15,22,24,25,26,32,33,34,35,38,42,50,76],proport:[10,16,25],props_se:10,provid:[16,32,45,47,74,76,79],proxim:13,ps1:68,psm1:68,ptrn_fanci:22,ptrn_plain:22,publish:4,purpl:11,purpos:[4,7,16,53,63,70,76],put:[35,76],py:[7,9,34],python3:68,python:[55,57,58,65,67,69,74,76],q1:45,q2:45,q:[10,11,45,74],qc:[45,76],qc_extract:[8,44],qmin:38,qname:[38,64,76],qnamesbatch:34,qnamesbatchio:[34,49,76],qq:[11,74],qual:[8,36,38,39],qualiti:[8,18,19,34,35,36,38,39,44,45,62,66],quality_cod:18,quantil:[16,17,31,32,42,44,45],quantiti:32,queri:76,quick:72,quiet:[11,74],quit:74,quotient:54,r2:76,r:[10,11,25,32,33,34,45,50],rais:[10,11,14,17,22,25,26,36,44,45,53,54,71,74],ram:70,randal:80,random:[7,10,21,25,26],randomli:[16,76],rang:[25,42,74],range_int:[25,42],range_on:25,rank:[10,38,56],rare:[16,47],rate:[10,16,17,24,31,32,44,45],rather:[15,20,38,52,69,80],ratio:[10,32,42,45],rational:71,raw:[11,76,78],rc:25,re:[8,22,44,45,62,66,69,70],reach:76,react:24,reactiv:[16,24,31],read1:38,read2:38,read:[8,9,10,13,15,16,18,22,25,28,33,34,36,38,39,42,44,45,46,48,52,53,55,56,57,62,63,64,66,69,72,80],read_1_id:54,read_2_id:54,read_bas:[22,38],read_dtyp:13,read_head:42,read_id_dict:28,read_index:13,read_nam:34,read_num:[10,13,33],read_nums_per_batch:10,read_qual:38,read_weight:[10,13],readabl:[11,35,58],readbatch:[13,15],readbatchio:[10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for Sequence Compression/Decompression","Algorithms","seismicrna package","seismicrna.align package","seismicrna.align.tests package","seismicrna.clust package","seismicrna.core package","seismicrna.core.arg package","seismicrna.core.batch package","seismicrna.core.extern package","seismicrna.core.io package","seismicrna.core.mu package","seismicrna.core.mu.tests package","seismicrna.core.ngs package","seismicrna.core.ngs.tests package","seismicrna.core.parallel package","seismicrna.core.parallel.tests package","seismicrna.core.rel package","seismicrna.core.rel.tests package","seismicrna.core.rna package","seismicrna.core.seq package","seismicrna.core.seq.tests package","seismicrna.core.tests package","seismicrna.demult package","seismicrna.export package","seismicrna.fastaclean package","seismicrna.fold package","seismicrna.graph package","seismicrna.mask package","seismicrna.relate package","seismicrna.relate.aux package","seismicrna.relate.aux.tests package","seismicrna.relate.c package","seismicrna.relate.py package","seismicrna.relate.py.tests package","seismicrna.sim package","seismicrna.sim.tests package","seismicrna.table package","seismicrna.test package","seismicrna.workflow package","Command Line Interface","Data Structures","Relation Vectors","Relate Data Structures","Read Names Batch","Reference Sequence","Relate Batch","Brickle: Compressed Python Objects","FASTA: Reference sequences","FASTQ: Sequencing Reads","Data Formats","SAM, BAM, and CRAM: Alignment Maps","File Formats","Metadata Formats","Metadata for References","Metadata for Samples","Metadata for Sections","Align Report","Report Formats","Relate Report","SEISMIC-RNA User Guide","Installing with Conda","Installation","Prerequisites: Python, pip, and Conda","Virtual Environments","Bugs and Requests","Cleaning FASTA Files","Manuals","Listing Input Files","Logging Messages","Parallelizing Tasks","Running the Workflow","Step-by-step walkthrough","Example dataset","Tutorials","How to Write this Manual"],titleterms:{"1":54,"1st":54,"2":[54,76],"256":78,"2nd":54,"3":51,"5":51,"export":29,"new":70,about:[3,59,60,61],activ:[66,69],adapt:76,algorithm:[5,6],align:[8,9,45,56,62,76],all:45,alphabet:54,ambigu:[47,76],ar:74,arg:12,argument:73,assess:76,author:0,aux:[35,36],auxiliari:35,background:[5,71],bam:56,base:[47,76],batch:[13,49,51,75,76],benefit:75,between:[47,76],bodi:53,bowti:76,brickl:52,bug:70,build:76,c:37,call:[47,76],can:76,charact:54,check:68,checksum:78,choos:76,clean:[30,71],clust:10,cluster:[10,45],coexist:69,come:[74,76],command:[12,45,59,60,61,71,76],comparison:[10,56],compress:[5,52],comput:78,conda:[66,68],conflict:69,consensu:47,content:[52,53,54,56],control:74,core:[11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27],cover:47,cram:56,creat:[66,69],curl:78,custom:76,cutadapt:76,cycl:76,dark:76,data:[46,48,55],dataset:78,deactiv:69,decompress:5,delet:[47,76],demult:[28,45],demultiplex:76,depend:66,dm:1,dna:54,download:78,dreem:2,each:76,effort:69,encod:[47,54,56,76],end:[47,51,54,76],ended:[54,56,76],enhanc:70,entir:76,environ:[66,69],error:71,everi:76,exampl:[5,49,50,51,59,60,61,62,64,78],expect:76,extens:[52,53,54,56],extern:14,fasta:[30,53,71],fastaclean:[30,45],fastq:[54,76],fastqc:76,featur:70,feedback:70,field:[59,60,61,62,64],file:[8,52,53,54,56,57,71,73,74,76,77,78],filter:76,five:74,fold:[31,45],format:[52,53,54,55,56,57,58,63,74,76],from:[76,78],further:76,gener:73,github:78,give:70,graph:[32,45],guid:65,guidelin:70,header:53,how:[69,70,74,76,80],illumina:76,index:76,initi:68,input:[52,53,54,56,73,76],insert:[47,76],instal:[66,67,68],interfac:[12,45],interleav:[54,76],io:15,irreconcil:47,iseq:76,iter:35,its:66,label:54,let:69,level:74,licens:4,line:[12,45,53,71],list:73,local:76,log:74,low:[47,76],lower:76,main:[7,34,44],manag:69,manual:[72,80],map:[56,76],mapseq:1,mask:[33,45],mate:54,md5:78,messag:74,metadata:[58,59,60,61],method:73,minimum:76,modul:[7,8,10,11,30,34,35,44],mu:[16,17],multipl:76,mutat:51,name:[10,49,76],nextseq:76,ng:[18,19],non:66,note:[59,60,61],number:[51,76],object:52,one:76,option:76,other:70,output:[52,53,54,56,74,76],packag:[7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44],pair:[47,54,76],parallel:[20,21,75],pars:[52,53,54,56],path:[52,53,54,56,74],phred:[54,76],pip:68,pipelin:44,plain:[59,60,61],posit:[47,51,73,76],pre:76,prerequisit:68,pretti:[59,60,61],primari:47,problem:70,procedur:[5,71],purpos:[11,59,60,61,71],py:[38,39],python:[52,66,68],qualiti:[47,54,56,76],random:11,rate:76,read:[35,47,49,51,54,76],real:74,refer:[47,50,53,59,76],rel:[22,23],relat:[34,35,36,37,38,39,45,47,48,51,64,76],relationship:47,report:[62,63,64,70,76],request:70,rna:[7,24,65,66],run:76,sam:[8,56],sampl:[60,76],scatter:45,score:[54,56,76],section:61,seismic:[7,45,65,66],seismicrna:[7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44],separ:[54,76],seq:[25,26],seqbar:45,seqcorr:45,seqdiff:45,sequenc:[5,50,53,54,76],set:[54,69,76,77],sha:78,share:76,sim:[40,41],simul:8,singl:[54,76],size:76,softwar:69,sofwar:69,sourc:76,specifi:76,standard:74,step:77,structur:[46,48,49,50,51],submodul:[7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,38,39,42,43,44],subpackag:[7,8,11,16,18,20,22,25,34,35,38,40],system:77,tabl:[42,45,59,60,61],task:75,temporari:[52,53,54,56],test:[9,17,19,21,23,26,27,36,39,41,43],text:[59,60,61],than:76,thi:80,time:74,trim:76,troubleshoot:[66,68,71,76],tutori:79,unalign:76,up:[69,77],url:78,us:[52,53,54,56,59,60,61,76,78],usag:71,user:65,vector:[34,47,76],verifi:[66,78],version:69,view:74,virtual:[66,69],walkthrough:77,wget:78,which:74,whole:[44,76],why:69,workflow:[44,76],worth:69,write:[34,80],xam:56,your:[66,68,77]}}) \ No newline at end of file diff --git a/src/userdocs/data/relate/codes.rst b/src/userdocs/data/relate/codes.rst index 04ab01c5..aa9d24ba 100644 --- a/src/userdocs/data/relate/codes.rst +++ b/src/userdocs/data/relate/codes.rst @@ -61,7 +61,9 @@ for some bases to define the relationship: - low-quality base calls - ambiguous insertions and deletions -Low-quality base calls +.. _relate_low_qual: + +Encoding low-quality base calls """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" A low-quality base call is defined as having a `Phred quality score`_ @@ -115,7 +117,7 @@ four columns "Read: A/C/G/T?". than once towards the total number of matches or mutations. To learn how mutations in relation vectors are counted, see [REF]. -Ambiguous insertions and deletions +Encoding ambiguous insertions and deletions """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" Insertions and deletions (collectively, "indels") in the read can cause @@ -183,7 +185,7 @@ is covered in one mate but not in the other, one mate's Phred score is above the threshold and the other's is below, or (more rarely) the base calls themselves differ. -Consensus relationships +Encoding consensus relationships """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" When finding the consensus of two mates, information in one mate should @@ -219,7 +221,7 @@ position 4 is a match, but it is low quality at position 5, so even the consensus byte is ambiguous. Neither mate covers position 6, so the consensus byte is blank. -Irreconcilable relationships +Encoding irreconcilable relationships """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" It is possible, although rare, for mates 1 and 2 to share no bits. For diff --git a/src/userdocs/manuals/workflow.rst b/src/userdocs/manuals/workflow.rst index 45afc2b0..008e84e8 100644 --- a/src/userdocs/manuals/workflow.rst +++ b/src/userdocs/manuals/workflow.rst @@ -633,7 +633,7 @@ matches or substitutions, as if they were ``N``\s. For example, if the minimum Phred score is 25 (the default) and a base ``T`` is called as a match with a Phred score of 20, then it would be marked as possibly a match and possibly a subsitution to A, C, or G. -See :doc:`../data/relate/codes` for more information. +See :ref:`relate_low_qual` for more information. Relate setting: Ambiguous insertions and deletions """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" @@ -704,8 +704,8 @@ See :doc:`../formats/data/brickle` for details on brickle files. Relate output file: Reference sequence """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" -The relate step writes the reference sequence as a ``CompressedSeq`` -object to the file ``refseq.brickle``. +The relate step writes the reference sequence as a ``RefseqIO`` object +to the file ``refseq.brickle``. See :doc:`../data/relate/refseq` for details on the data structure. See :doc:`../formats/data/brickle` for details on brickle files. From d5dc966cb1a594ad02d6595607c262bd2b18377a Mon Sep 17 00:00:00 2001 From: justinaruda Date: Fri, 17 Nov 2023 12:35:40 -0500 Subject: [PATCH 6/9] Optimized masking by replacing np.intersect1d() with np.setdiff1d(). --- src/seismicrna/mask/batch.py | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/src/seismicrna/mask/batch.py b/src/seismicrna/mask/batch.py index 77ac3423..b98d0a41 100644 --- a/src/seismicrna/mask/batch.py +++ b/src/seismicrna/mask/batch.py @@ -27,6 +27,8 @@ class MaskMutsBatch(MaskReadBatch, RefseqMutsBatch, PartialMutsBatch, ABC): def apply_mask(batch: RefseqMutsBatch, reads: Iterable[int] | None = None, positions: Iterable[int] | None = None): + # Determine masked read numbers. + masked_reads = np.setdiff1d(batch.read_nums, reads) # Clean and validate the selection. if positions is not None: positions = sanitize_pos(positions, batch.max_pos) @@ -34,10 +36,10 @@ def apply_mask(batch: RefseqMutsBatch, muts = dict() for pos in positions if positions is not None else batch.pos_nums: muts[pos] = dict() - # Select reads with each type of mutation at this position. + # Remove masked reads with each type of mutation at this position. for mut, pos_mut_reads in batch.muts.get(pos, dict()).items(): - muts[pos][mut] = (np.intersect1d(pos_mut_reads, - reads, + muts[pos][mut] = (np.setdiff1d(pos_mut_reads, + masked_reads, assume_unique=True) if reads is not None else pos_mut_reads) From bb8ed3d2beabf6bb22d991d837d41369ae5699f5 Mon Sep 17 00:00:00 2001 From: justinaruda Date: Fri, 17 Nov 2023 13:37:56 -0500 Subject: [PATCH 7/9] Added a conditional check to the definition of masked_reads during masking for consistent logic. --- src/seismicrna/mask/batch.py | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/src/seismicrna/mask/batch.py b/src/seismicrna/mask/batch.py index b98d0a41..a3f14072 100644 --- a/src/seismicrna/mask/batch.py +++ b/src/seismicrna/mask/batch.py @@ -27,8 +27,10 @@ class MaskMutsBatch(MaskReadBatch, RefseqMutsBatch, PartialMutsBatch, ABC): def apply_mask(batch: RefseqMutsBatch, reads: Iterable[int] | None = None, positions: Iterable[int] | None = None): - # Determine masked read numbers. - masked_reads = np.setdiff1d(batch.read_nums, reads) + # Determine masked read numbers. + masked_reads = (np.setdiff1d(batch.read_nums, reads) + if reads is not None + else None) # Clean and validate the selection. if positions is not None: positions = sanitize_pos(positions, batch.max_pos) From 7de032529178e67640a00c06df3c5b8c98792f6e Mon Sep 17 00:00:00 2001 From: matthewfallan <31744230+matthewfallan@users.noreply.github.com> Date: Fri, 17 Nov 2023 14:31:22 -0500 Subject: [PATCH 8/9] Rename parameter "overwrite" to "force" --- src/seismicrna/align/fqops.py | 1 + src/seismicrna/clust/write.py | 2 +- src/seismicrna/core/io/brickle.py | 10 ++++++---- src/seismicrna/core/io/file.py | 13 ++++--------- src/seismicrna/core/seq/fasta.py | 7 ++++--- src/seismicrna/core/seq/tests/fasta_test.py | 2 +- src/seismicrna/mask/write.py | 2 +- src/seismicrna/relate/write.py | 2 +- 8 files changed, 19 insertions(+), 20 deletions(-) diff --git a/src/seismicrna/align/fqops.py b/src/seismicrna/align/fqops.py index 595e2f60..c2e8088d 100755 --- a/src/seismicrna/align/fqops.py +++ b/src/seismicrna/align/fqops.py @@ -12,6 +12,7 @@ def fastqc_cmd(fq_unit: FastqUnit, extract: bool, n_procs: int): args = [FASTQC_CMD, + "--quiet", "--threads", n_procs, "--extract" if extract else "--noextract", "--outdir", out_prefix.parent] diff --git a/src/seismicrna/clust/write.py b/src/seismicrna/clust/write.py index d99dda12..a42eb07b 100644 --- a/src/seismicrna/clust/write.py +++ b/src/seismicrna/clust/write.py @@ -39,7 +39,7 @@ def write_batches(dataset: MaskMerger, resps=resps) _, checksum = batch.save(top=dataset.top, brotli_level=brotli_level, - overwrite=True) + force=True) checksums.append(checksum) return checksums diff --git a/src/seismicrna/core/io/brickle.py b/src/seismicrna/core/io/brickle.py index 6a820cb9..2890ba96 100644 --- a/src/seismicrna/core/io/brickle.py +++ b/src/seismicrna/core/io/brickle.py @@ -6,6 +6,8 @@ import brotli +from ..write import write_mode + logger = getLogger(__name__) DEFAULT_BROTLI_LEVEL = 10 @@ -20,13 +22,13 @@ def digest_data(data: bytes): def save_brickle(item: Any, file: Path, brotli_level: int = DEFAULT_BROTLI_LEVEL, - overwrite: bool = False): + force: bool = False): """ Pickle an object, compress with Brotli, and save to a file. """ data = brotli.compress(pickle.dumps(item, protocol=PICKLE_PROTOCOL), quality=brotli_level) - with open(file, 'wb' if overwrite else 'xb') as f: + with open(file, write_mode(force, binary=True)) as f: f.write(data) - logger.debug(f"Wrote {item} to {file} (Brotli level: {brotli_level})") + logger.debug(f"Wrote {item} (brotli level {brotli_level}) to {file}") checksum = digest_data(data) logger.debug(f"Computed MD5 checksum of {file}: {checksum}") return checksum @@ -36,7 +38,7 @@ def load_brickle(file: Path, checksum: str, check_type: None | type | tuple[type, ...] = None): """ Unpickle and return an object from a Brotli-compressed file. """ - with open(file, 'rb') as f: + with open(file, "rb") as f: data = f.read() if checksum != (digest := digest_data(data)): raise ValueError( diff --git a/src/seismicrna/core/io/file.py b/src/seismicrna/core/io/file.py index 3c7950f0..646b3b19 100644 --- a/src/seismicrna/core/io/file.py +++ b/src/seismicrna/core/io/file.py @@ -126,17 +126,12 @@ class BrickleIO(FileIO, ABC): @classmethod def load(cls, file: Path, checksum: str = ""): """ Load from a compressed pickle file. """ - return load_brickle(file, check_type=cls, checksum=checksum) + return load_brickle(file, checksum=checksum, check_type=cls) - def save(self, - top: Path, - brotli_level: int = DEFAULT_BROTLI_LEVEL, - overwrite: bool = False): + def save(self, top: Path, *args, **kwargs): """ Save to a pickle file compressed with Brotli. """ - checksum = save_brickle(self, - save_path := self.get_path(top), - brotli_level=brotli_level, - overwrite=overwrite) + save_path = self.get_path(top) + checksum = save_brickle(self, save_path, *args, **kwargs) return save_path, checksum def __getstate__(self): diff --git a/src/seismicrna/core/seq/fasta.py b/src/seismicrna/core/seq/fasta.py index e428e220..be22656c 100644 --- a/src/seismicrna/core/seq/fasta.py +++ b/src/seismicrna/core/seq/fasta.py @@ -4,8 +4,9 @@ from pathlib import Path from typing import Iterable -from ..path import STR_CHARS from .xna import XNA +from ..path import STR_CHARS +from ..write import write_mode logger = getLogger(__name__) @@ -126,13 +127,13 @@ def get_fasta_seq(fasta: Path, seq_type: type[XNA], name: str): def write_fasta(fasta: Path, refs: Iterable[tuple[str, XNA]], wrap: int = 0, - overwrite: bool = False): + force: bool = False): """ Write an iterable of reference names and DNA sequences to a FASTA file. """ logger.info(f"Began writing FASTA file: {fasta}") # Record the names of all the references. names = set() - with open(fasta, 'w' if overwrite else 'x') as f: + with open(fasta, write_mode(force)) as f: for name, seq in refs: try: # Confirm that the name is not blank. diff --git a/src/seismicrna/core/seq/tests/fasta_test.py b/src/seismicrna/core/seq/tests/fasta_test.py index 6ebe77a2..91081cd1 100644 --- a/src/seismicrna/core/seq/tests/fasta_test.py +++ b/src/seismicrna/core/seq/tests/fasta_test.py @@ -241,7 +241,7 @@ def test_overwrite(self): text = ">Seq1\nGTACGTGNTCATC\n>Seq2\nAGCTGTGNNTATCG\n" with NTFile('w', suffix=path.FASTA_EXTS[0], delete=False) as f: filepath = Path(f.file.name) - write_fasta(filepath, seqs, overwrite=True) + write_fasta(filepath, seqs, force=True) with open(filepath) as f: self.assertEqual(f.read(), text) remove(filepath) diff --git a/src/seismicrna/mask/write.py b/src/seismicrna/mask/write.py index 12264034..7d97cd99 100644 --- a/src/seismicrna/mask/write.py +++ b/src/seismicrna/mask/write.py @@ -262,7 +262,7 @@ def _filter_batch_reads(self, batch: RefseqMutsBatch): read_nums=batch.read_nums) _, checksum = batch_file.save(self.top, brotli_level=self.brotli_level, - overwrite=True) + force=True) self.checksums.append(checksum) return batch diff --git a/src/seismicrna/relate/write.py b/src/seismicrna/relate/write.py index a8213d83..e855850c 100644 --- a/src/seismicrna/relate/write.py +++ b/src/seismicrna/relate/write.py @@ -130,7 +130,7 @@ def _write_refseq(self, out_dir: Path, brotli_level: int): refseq_file = RefseqIO(sample=self.sample, ref=self.ref, refseq=self.seq) - _, checksum = refseq_file.save(out_dir, brotli_level, overwrite=True) + _, checksum = refseq_file.save(out_dir, brotli_level, force=True) return checksum def _generate_batches(self, *, From e458f3896a7af56afe645bc309cb4791ae640a5b Mon Sep 17 00:00:00 2001 From: matthewfallan <31744230+matthewfallan@users.noreply.github.com> Date: Fri, 17 Nov 2023 14:32:26 -0500 Subject: [PATCH 9/9] Bump version 0.9.4 -> 0.9.5 --- src/seismicrna/core/version.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/src/seismicrna/core/version.py b/src/seismicrna/core/version.py index 805c6335..2307e0af 100644 --- a/src/seismicrna/core/version.py +++ b/src/seismicrna/core/version.py @@ -7,9 +7,9 @@ logger = getLogger(__name__) -__version__ = "0.9.4" +__version__ = "0.9.5" -VERSION_DELIM = '.' +VERSION_DELIM = "." 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