From 4e6c28112625b2fb0d12ab93439c73143689e5ae Mon Sep 17 00:00:00 2001
From: matthewfallan <31744230+matthewfallan@users.noreply.github.com>
Date: Sat, 13 Jan 2024 21:53:56 -0500
Subject: [PATCH] Update docs on +addclust

---
 docs/.doctrees/api/seismicrna.cluster.doctree | Bin 281685 -> 325087 bytes
 docs/.doctrees/api/seismicrna.core.doctree    | Bin 701518 -> 704211 bytes
 docs/.doctrees/api/seismicrna.core.io.doctree | Bin 107605 -> 120662 bytes
 .../api/seismicrna.core.io.tests.doctree      | Bin 0 -> 20420 bytes
 docs/.doctrees/api/seismicrna.core.mu.doctree | Bin 187646 -> 197265 bytes
 docs/.doctrees/api/seismicrna.wf.doctree      | Bin 250891 -> 251003 bytes
 docs/.doctrees/environment.pickle             | Bin 3269709 -> 3283631 bytes
 docs/.doctrees/howto/addclust.doctree         | Bin 0 -> 13431 bytes
 docs/.doctrees/howto/index.doctree            | Bin 2839 -> 2861 bytes
 docs/.doctrees/howto/run/cluster.doctree      | Bin 50550 -> 53377 bytes
 docs/_sources/api/seismicrna.cluster.rst.txt  |  12 +
 docs/_sources/api/seismicrna.core.io.rst.txt  |   8 +
 .../api/seismicrna.core.io.tests.rst.txt      |  16 ++
 docs/_sources/howto/addclust.rst.txt          |  57 +++++
 docs/_sources/howto/index.rst.txt             |   1 +
 docs/_sources/howto/run/cluster.rst.txt       |  11 +
 docs/api/seismicrna.cluster.html              | 141 +++++++-----
 docs/api/seismicrna.core.html                 |  36 ++-
 docs/api/seismicrna.core.io.html              |  27 ++-
 docs/api/seismicrna.core.io.tests.html        | 205 ++++++++++++++++++
 docs/api/seismicrna.core.mu.html              |  33 ++-
 docs/api/seismicrna.html                      |   1 +
 docs/api/seismicrna.wf.html                   |   4 +-
 docs/genindex.html                            | 122 +++++++++--
 docs/howto/addclust.html                      | 192 ++++++++++++++++
 docs/howto/cleanfa.html                       |   5 +-
 docs/howto/graph/index.html                   |   1 +
 docs/howto/graph/params.html                  |   1 +
 docs/howto/graph/profile.html                 |   1 +
 docs/howto/index.html                         |   6 +
 docs/howto/inputs.html                        |   1 +
 docs/howto/logging.html                       |   5 +-
 docs/howto/normalize.html                     |   1 +
 docs/howto/parallel.html                      |   1 +
 docs/howto/run/align.html                     |   1 +
 docs/howto/run/cluster.html                   |  12 +-
 docs/howto/run/demult.html                    |   1 +
 docs/howto/run/export.html                    |   1 +
 docs/howto/run/fold.html                      |   1 +
 docs/howto/run/graph.html                     |   1 +
 docs/howto/run/index.html                     |   1 +
 docs/howto/run/mask.html                      |   1 +
 docs/howto/run/pool.html                      |   1 +
 docs/howto/run/relate.html                    |   1 +
 docs/howto/run/table.html                     |   1 +
 docs/howto/run/wf.html                        |   1 +
 docs/howto/sections.html                      |   1 +
 docs/index.html                               |   1 +
 docs/objects.inv                              | Bin 33383 -> 33881 bytes
 docs/py-modindex.html                         |  20 ++
 docs/searchindex.js                           |   2 +-
 src/docs/api/seismicrna.cluster.rst           |  12 +
 src/docs/api/seismicrna.core.io.rst           |   8 +
 src/docs/api/seismicrna.core.io.tests.rst     |  16 ++
 src/docs/howto/addclust.rst                   |  57 +++++
 src/docs/howto/index.rst                      |   1 +
 src/docs/howto/run/cluster.rst                |  11 +
 57 files changed, 945 insertions(+), 95 deletions(-)
 create mode 100644 docs/.doctrees/api/seismicrna.core.io.tests.doctree
 create mode 100644 docs/.doctrees/howto/addclust.doctree
 create mode 100644 docs/_sources/api/seismicrna.core.io.tests.rst.txt
 create mode 100644 docs/_sources/howto/addclust.rst.txt
 create mode 100644 docs/api/seismicrna.core.io.tests.html
 create mode 100644 docs/howto/addclust.html
 create mode 100644 src/docs/api/seismicrna.core.io.tests.rst
 create mode 100644 src/docs/howto/addclust.rst

diff --git a/docs/.doctrees/api/seismicrna.cluster.doctree b/docs/.doctrees/api/seismicrna.cluster.doctree
index 162b9967b677138f7e6cf19f24616d09a0f18406..f8345e0f0c65a93d0f63462ab1c0c505ce43e6b5 100644
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diff --git a/docs/_sources/api/seismicrna.cluster.rst.txt b/docs/_sources/api/seismicrna.cluster.rst.txt
index 09efaccd..15e06826 100644
--- a/docs/_sources/api/seismicrna.cluster.rst.txt
+++ b/docs/_sources/api/seismicrna.cluster.rst.txt
@@ -64,12 +64,24 @@ Submodules
    :show-inheritance:
 
 
+.. automodule:: seismicrna.cluster.save
+   :members:
+   :undoc-members:
+   :show-inheritance:
+
+
 .. automodule:: seismicrna.cluster.uniq
    :members:
    :undoc-members:
    :show-inheritance:
 
 
+.. automodule:: seismicrna.cluster.update
+   :members:
+   :undoc-members:
+   :show-inheritance:
+
+
 .. automodule:: seismicrna.cluster.write
    :members:
    :undoc-members:
diff --git a/docs/_sources/api/seismicrna.core.io.rst.txt b/docs/_sources/api/seismicrna.core.io.rst.txt
index bffcb1df..771dea99 100644
--- a/docs/_sources/api/seismicrna.core.io.rst.txt
+++ b/docs/_sources/api/seismicrna.core.io.rst.txt
@@ -6,6 +6,14 @@ seismicrna.core.io package
    :undoc-members:
    :show-inheritance:
 
+Subpackages
+-----------
+
+.. toctree::
+   :maxdepth: 4
+
+   seismicrna.core.io.tests
+
 Submodules
 ----------
 
diff --git a/docs/_sources/api/seismicrna.core.io.tests.rst.txt b/docs/_sources/api/seismicrna.core.io.tests.rst.txt
new file mode 100644
index 00000000..a2c6687a
--- /dev/null
+++ b/docs/_sources/api/seismicrna.core.io.tests.rst.txt
@@ -0,0 +1,16 @@
+seismicrna.core.io.tests package
+================================
+
+.. automodule:: seismicrna.core.io.tests
+   :members:
+   :undoc-members:
+   :show-inheritance:
+
+Submodules
+----------
+
+
+.. automodule:: seismicrna.core.io.tests.file_test
+   :members:
+   :undoc-members:
+   :show-inheritance:
diff --git a/docs/_sources/howto/addclust.rst.txt b/docs/_sources/howto/addclust.rst.txt
new file mode 100644
index 00000000..d0f84952
--- /dev/null
+++ b/docs/_sources/howto/addclust.rst.txt
@@ -0,0 +1,57 @@
+
+Add Clusters to an Already-Clustered Dataset
+--------------------------------------------------------------------------------
+
+Background about adding clusters
+^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
+
+Purpose of adding clusters
+""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
+
+After running the Cluster step, you may want to continue clustering using more
+clusters.
+You could accomplish this by simply rerunning the Cluster step using a larger
+``--max-clusters`` (``-k``).
+But since Cluster always begins with 1 cluster, you would need to repeat all the
+clusters that you had already run before being able to add more clusters, which
+would waste your computer resources.
+The Add Clusters tool lets you keep your existing clustering results and merely
+append more clusters, which is faster and more efficient.
+
+How to add clusters to a Cluster dataset
+^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
+
+Command line for adding clusters
+""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
+
+Suppose you have already run clustering with a maximum of 2 clusters (``-k 2``)
+on a dataset::
+
+    seismic cluster -k 2 {out}/{sample}/mask/{ref}/{sect}
+
+To add more clusters, use ``seismic +addclust`` with the new maximum number of
+clusters (e.g. 4) and the Cluster report as the input file::
+
+    seismic +addclust -k 4 {out}/{sample}/cluster/{ref}/{sect}
+
+This command will resume clustering with one more than the maximum number of
+existing clusters (in this case, the previous maximum was 2, so ``+addclust``
+will begin at 3).
+The maximum number of clusters follows the same rules as in ``cluster`` (see
+:ref:`cluster_max` for more information).
+You do not need to specify any other settings for clustering, such as the number
+of runs or the threshold for convergence: ``+addclust`` automatically uses the
+same settings that you used originally in ``cluster``.
+
+You can give any number of Cluster report files as inputs for Add Clusters.
+See :doc:`./inputs` for ways to list multiple files.
+
+The only new files produced are ``mus`` and ``props`` files for each additional
+clustering run.
+The Cluster report file, the batch files, and the counts file are all updated
+in-place, as if you had run ``cluster`` the first time with the new ``-k``.
+Because updating in-place means that there is a risk of data loss if any error
+occurs during writing, the original files are all first backed up in a temporary
+directory, which you can specify with ``--temp-dir`` (``-t``).
+In case of a fatal error while updating the files, the original files will all
+be restored from the backup, as if you had never run ``+addclust``.
diff --git a/docs/_sources/howto/index.rst.txt b/docs/_sources/howto/index.rst.txt
index 21648e5e..dd67a37c 100644
--- a/docs/_sources/howto/index.rst.txt
+++ b/docs/_sources/howto/index.rst.txt
@@ -12,4 +12,5 @@ How To
     normalize
     parallel
     logging
+    addclust
     cleanfa
diff --git a/docs/_sources/howto/run/cluster.rst.txt b/docs/_sources/howto/run/cluster.rst.txt
index 36c1bed1..0c495b19 100644
--- a/docs/_sources/howto/run/cluster.rst.txt
+++ b/docs/_sources/howto/run/cluster.rst.txt
@@ -26,6 +26,8 @@ To cluster all masked relation vectors in ``{out}``, you can use the command ::
 Cluster: Settings
 ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
 
+.. _cluster_max:
+
 Cluster setting: Maximum number of clusters
 """"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
 
@@ -57,6 +59,10 @@ This process continues until one of two limits is reached:
     the number that yielded the best BIC (field "Optimal Number of Clusters"),
     which is less than or equal to the maximum you specified.
 
+.. note::
+    If you decide after clustering that you wanted to run clustering with more
+    clusters, then use the tool ``+addclust`` (see :doc:`../addclust`).
+
 Cluster setting: Expectation-maximization iterations
 """"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
 
@@ -175,6 +181,11 @@ In your cluster report:
 Cluster: Troubleshoot and optimize
 ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
 
+Run Cluster with more clusters, without repeating the work already done
+""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
+
+The tool ``+addclust`` exists for this purpose: see :doc:`../addclust`.
+
 Cluster takes too long to finish
 """"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
 
diff --git a/docs/api/seismicrna.cluster.html b/docs/api/seismicrna.cluster.html
index 263c0d32..7c34449e 100644
--- a/docs/api/seismicrna.cluster.html
+++ b/docs/api/seismicrna.cluster.html
@@ -152,60 +152,54 @@ <h3>Cluster Comparison Module<a class="headerlink" href="#cluster-comparison-mod
 <em class="property"><span class="pre">class</span><span class="w"> </span></em><span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">RunOrderResults</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.em.EmClustering" title="seismicrna.cluster.em.EmClustering"><span class="pre">seismicrna.cluster.em.EmClustering</span></a><span class="p"><span class="pre">]</span></span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.RunOrderResults" title="Permalink to this definition"></a></dt>
 <dd><p>Bases: <a class="reference external" href="https://docs.python.org/3/library/functions.html#object" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">object</span></code></a></p>
 <p>Results of clustering runs of the same order.</p>
+<dl class="py property">
+<dt class="sig sig-object py" id="seismicrna.cluster.compare.RunOrderResults.bic">
+<em class="property"><span class="pre">property</span><span class="w"> </span></em><span class="sig-name descname"><span class="pre">bic</span></span><a class="headerlink" href="#seismicrna.cluster.compare.RunOrderResults.bic" title="Permalink to this definition"></a></dt>
+<dd><p>BIC of the best run.</p>
+</dd></dl>
+
 </dd></dl>
 
 <dl class="py function">
-<dt class="sig sig-object py" id="seismicrna.cluster.compare.calc_information_entropy">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">calc_information_entropy</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">x</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">validate</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.calc_information_entropy" title="Permalink to this definition"></a></dt>
-<dd><p>Calculate the information entropy of a set of observations, x.</p>
+<dt class="sig sig-object py" id="seismicrna.cluster.compare.assign_clusterings">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">assign_clusterings</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">mus1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">mus2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.assign_clusterings" title="Permalink to this definition"></a></dt>
+<dd><p>Optimally assign clusters from two groups to each other.</p>
 </dd></dl>
 
 <dl class="py function">
-<dt class="sig sig-object py" id="seismicrna.cluster.compare.calc_mean_var_info">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">calc_mean_var_info</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.em.EmClustering" title="seismicrna.cluster.em.EmClustering"><span class="pre">seismicrna.cluster.em.EmClustering</span></a><span class="p"><span class="pre">]</span></span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.calc_mean_var_info" title="Permalink to this definition"></a></dt>
-<dd><p>Calculate the expected variation of information among ≥ 2 runs
-of EM clustering.</p>
+<dt class="sig sig-object py" id="seismicrna.cluster.compare.calc_mean_pearson">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">calc_mean_pearson</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">run1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.em.EmClustering" title="seismicrna.cluster.em.EmClustering"><span class="pre">seismicrna.cluster.em.EmClustering</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">run2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.em.EmClustering" title="seismicrna.cluster.em.EmClustering"><span class="pre">seismicrna.cluster.em.EmClustering</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.calc_mean_pearson" title="Permalink to this definition"></a></dt>
+<dd><p>Compute the mean Pearson correlation between the clusters.</p>
 </dd></dl>
 
 <dl class="py function">
-<dt class="sig sig-object py" id="seismicrna.cluster.compare.calc_var_info_pair">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">calc_var_info_pair</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">resps1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">pandas.core.frame.DataFrame</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">resps2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">pandas.core.frame.DataFrame</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.calc_var_info_pair" title="Permalink to this definition"></a></dt>
-<dd><p>Calculate the variation of information for a pair of clustering
-results.</p>
+<dt class="sig sig-object py" id="seismicrna.cluster.compare.calc_nrmsd_groups">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">calc_nrmsd_groups</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">mus1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">mus2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.calc_nrmsd_groups" title="Permalink to this definition"></a></dt>
+<dd><p>Calculate the NRMSD of each pair of clusters in two groups.</p>
 </dd></dl>
 
 <dl class="py function">
-<dt class="sig sig-object py" id="seismicrna.cluster.compare.calc_var_info_pqr">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">calc_var_info_pqr</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">x</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">y</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">xy</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">validate</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.calc_var_info_pqr" title="Permalink to this definition"></a></dt>
-<dd><p>Calculate the variation of information for two partitions, X and Y,
-of the same set A. For more details and the source of the formula,
-see <a class="reference external" href="https://en.wikipedia.org/wiki/Variation_of_information">https://en.wikipedia.org/wiki/Variation_of_information</a>.</p>
-<dl class="field-list simple">
-<dt class="field-odd">Parameters</dt>
-<dd class="field-odd"><ul class="simple">
-<li><p><strong>x</strong> (<code class="xref py py-class docutils literal notranslate"><span class="pre">ndarray</span></code>) – A 1-dimensional array of the fraction of the total elements in
-each partition of X. All must be in (0, 1] and sum to 1.</p></li>
-<li><p><strong>y</strong> (<code class="xref py py-class docutils literal notranslate"><span class="pre">ndarray</span></code>) – A 1-dimensional array of the fraction of the total elements in
-each partition of Y. All must be in (0, 1] and sum to 1.</p></li>
-<li><p><strong>xy</strong> (<code class="xref py py-class docutils literal notranslate"><span class="pre">ndarray</span></code>) – A 2-dimensional array (len(p) x len(q)) of the fraction of the
-total elements that are in each pair of partitions from X and Y.
-All must be in (0, 1] and sum to 1.</p></li>
-<li><p><strong>validate</strong> (<code class="xref py py-class docutils literal notranslate"><span class="pre">bool</span> <span class="pre">=</span> <span class="pre">True</span></code>) – Whether to validate the values of p, q, and r before calculating
-the variation of information.</p></li>
-</ul>
-</dd>
-<dt class="field-even">Returns</dt>
-<dd class="field-even"><p>Variation of information</p>
-</dd>
-<dt class="field-odd">Return type</dt>
-<dd class="field-odd"><p><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">float</span></code></a></p>
-</dd>
-</dl>
+<dt class="sig sig-object py" id="seismicrna.cluster.compare.calc_pearson_groups">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">calc_pearson_groups</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">mus1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">mus2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.calc_pearson_groups" title="Permalink to this definition"></a></dt>
+<dd><p>Calculate the Pearson correlation of each pair of clusters in
+two groups.</p>
+</dd></dl>
+
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.cluster.compare.calc_rms_nrmsd">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">calc_rms_nrmsd</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">run1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.em.EmClustering" title="seismicrna.cluster.em.EmClustering"><span class="pre">seismicrna.cluster.em.EmClustering</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">run2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.em.EmClustering" title="seismicrna.cluster.em.EmClustering"><span class="pre">seismicrna.cluster.em.EmClustering</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.calc_rms_nrmsd" title="Permalink to this definition"></a></dt>
+<dd><p>Compute the root-mean-square NRMSD between the clusters.</p>
+</dd></dl>
+
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.cluster.compare.calc_rmsd_groups">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">calc_rmsd_groups</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">mus1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">mus2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.calc_rmsd_groups" title="Permalink to this definition"></a></dt>
+<dd><p>Calculate the RMSD of each pair of clusters in two groups.</p>
 </dd></dl>
 
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.cluster.compare.find_best_order">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">find_best_order</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">ord_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#dict" title="(in Python v3.12)"><span class="pre">dict</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em><span class="sig-paren">)</span> <span class="sig-return"><span class="sig-return-icon">&#x2192;</span> <span class="sig-return-typehint"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></span><a class="headerlink" href="#seismicrna.cluster.compare.find_best_order" title="Permalink to this definition"></a></dt>
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">find_best_order</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">orders</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em><span class="sig-paren">)</span> <span class="sig-return"><span class="sig-return-icon">&#x2192;</span> <span class="sig-return-typehint"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></span><a class="headerlink" href="#seismicrna.cluster.compare.find_best_order" title="Permalink to this definition"></a></dt>
 <dd><p>Find the number of clusters with the best (smallest) BIC.</p>
 </dd></dl>
 
@@ -216,7 +210,7 @@ <h3>Cluster Comparison Module<a class="headerlink" href="#cluster-comparison-mod
 
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.cluster.compare.get_common_best_run_attr">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">get_common_best_run_attr</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">ord_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#dict" title="(in Python v3.12)"><span class="pre">dict</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">attr</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.get_common_best_run_attr" title="Permalink to this definition"></a></dt>
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">get_common_best_run_attr</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">orders</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">attr</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.get_common_best_run_attr" title="Permalink to this definition"></a></dt>
 <dd><p>Get an attribute of the best clustering run from every order,
 and confirm that <cite>key(attribute)</cite> is identical for all orders.</p>
 </dd></dl>
@@ -231,7 +225,7 @@ <h3>Cluster Comparison Module<a class="headerlink" href="#cluster-comparison-mod
 
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.cluster.compare.get_log_exp_obs_counts">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">get_log_exp_obs_counts</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">ord_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#dict" title="(in Python v3.12)"><span class="pre">dict</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.get_log_exp_obs_counts" title="Permalink to this definition"></a></dt>
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.compare.</span></span><span class="sig-name descname"><span class="pre">get_log_exp_obs_counts</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">orders</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.compare.get_log_exp_obs_counts" title="Permalink to this definition"></a></dt>
 <dd><p>Get the expected and observed log counts of each bit vector.</p>
 </dd></dl>
 
@@ -255,9 +249,15 @@ <h3>Cluster Comparison Module<a class="headerlink" href="#cluster-comparison-mod
 <dd><p>Build a path for a table of clustering results.</p>
 </dd></dl>
 
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.cluster.csv.update_log_counts">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.csv.</span></span><span class="sig-name descname"><span class="pre">update_log_counts</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">orders</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">top</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">sample</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">ref</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">sect</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.csv.update_log_counts" title="Permalink to this definition"></a></dt>
+<dd><p>Update the expected log counts of unique bit vectors.</p>
+</dd></dl>
+
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.cluster.csv.write_log_counts">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.csv.</span></span><span class="sig-name descname"><span class="pre">write_log_counts</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">ord_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#dict" title="(in Python v3.12)"><span class="pre">dict</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">top</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">sample</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">ref</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">sect</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.csv.write_log_counts" title="Permalink to this definition"></a></dt>
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.csv.</span></span><span class="sig-name descname"><span class="pre">write_log_counts</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">orders</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">top</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">sample</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">ref</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">sect</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.csv.write_log_counts" title="Permalink to this definition"></a></dt>
 <dd><p>Write the expected and observed log counts of unique bit vectors
 to a CSV file.</p>
 </dd></dl>
@@ -527,7 +527,7 @@ <h3>Cluster Comparison Module<a class="headerlink" href="#cluster-comparison-mod
 
 <span class="target" id="module-seismicrna.cluster.main"></span><dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.cluster.main.run">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.main.</span></span><span class="sig-name descname"><span class="pre">run</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">input_path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_clusters</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">em_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">6</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_em_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">8</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_em_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">512</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">em_thresh</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.01</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">10</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_procs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">16</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">parallel</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">force</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em><span class="sig-paren">)</span> <span class="sig-return"><span class="sig-return-icon">&#x2192;</span> <span class="sig-return-typehint"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a><span class="p"><span class="pre">]</span></span></span></span><a class="headerlink" href="#seismicrna.cluster.main.run" title="Permalink to this definition"></a></dt>
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.main.</span></span><span class="sig-name descname"><span class="pre">run</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">input_path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_clusters</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">em_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">6</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_em_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">8</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_em_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">512</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">em_thresh</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.6931471805599453</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">10</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_procs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">16</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">parallel</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">force</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em><span class="sig-paren">)</span> <span class="sig-return"><span class="sig-return-icon">&#x2192;</span> <span class="sig-return-typehint"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a><span class="p"><span class="pre">]</span></span></span></span><a class="headerlink" href="#seismicrna.cluster.main.run" title="Permalink to this definition"></a></dt>
 <dd><p>Infer alternative structures by clustering reads’ mutations.</p>
 <dl class="field-list simple">
 <dt class="field-odd">Parameters</dt>
@@ -536,7 +536,7 @@ <h3>Cluster Comparison Module<a class="headerlink" href="#cluster-comparison-mod
 <li><p><strong>em_runs</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Number of independent runs for each number of clusters [keyword-only, default: 6]</p></li>
 <li><p><strong>min_em_iter</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Minimum iterations per clustering run [keyword-only, default: 8]</p></li>
 <li><p><strong>max_em_iter</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Maximum iterations per clustering run [keyword-only, default: 512]</p></li>
-<li><p><strong>em_thresh</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">float</span></code></a>) – Maximum change in log likelihood for convergence [keyword-only, default: 0.01]</p></li>
+<li><p><strong>em_thresh</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">float</span></code></a>) – Maximum change in log likelihood for convergence [keyword-only, default: 0.6931471805599453]</p></li>
 <li><p><strong>brotli_level</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Compression level for brotli (0 - 11) [keyword-only, default: 10]</p></li>
 <li><p><strong>max_procs</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Maximum number of simultaneous processes [keyword-only, default: 16]</p></li>
 <li><p><strong>parallel</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">bool</span></code></a>) – Run tasks in parallel [keyword-only, default: True]</p></li>
@@ -575,7 +575,7 @@ <h3>Cluster – Names Module<a class="headerlink" href="#cluster-names-module" t
 
 <dl class="py method">
 <dt class="sig sig-object py" id="seismicrna.cluster.report.ClustReport.from_clusters">
-<em class="property"><span class="pre">classmethod</span><span class="w"> </span></em><span class="sig-name descname"><span class="pre">from_clusters</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">ord_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#dict" title="(in Python v3.12)"><span class="pre">dict</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">uniq_reads</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.uniq.UniqReads" title="seismicrna.cluster.uniq.UniqReads"><span class="pre">seismicrna.cluster.uniq.UniqReads</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_order</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">num_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">conv_thresh</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">checksums</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">began</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/datetime.html#datetime.datetime" title="(in Python v3.12)"><span class="pre">datetime.datetime</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">ended</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/datetime.html#datetime.datetime" title="(in Python v3.12)"><span class="pre">datetime.datetime</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.report.ClustReport.from_clusters" title="Permalink to this definition"></a></dt>
+<em class="property"><span class="pre">classmethod</span><span class="w"> </span></em><span class="sig-name descname"><span class="pre">from_clusters</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">orders</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">uniq_reads</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.uniq.UniqReads" title="seismicrna.cluster.uniq.UniqReads"><span class="pre">seismicrna.cluster.uniq.UniqReads</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_order</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">num_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">conv_thresh</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">checksums</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">began</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/datetime.html#datetime.datetime" title="(in Python v3.12)"><span class="pre">datetime.datetime</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">ended</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/datetime.html#datetime.datetime" title="(in Python v3.12)"><span class="pre">datetime.datetime</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.report.ClustReport.from_clusters" title="Permalink to this definition"></a></dt>
 <dd><p>Create a ClusterReport from EmClustering objects.</p>
 </dd></dl>
 
@@ -591,6 +591,18 @@ <h3>Cluster – Names Module<a class="headerlink" href="#cluster-names-module" t
 
 </dd></dl>
 
+<span class="target" id="module-seismicrna.cluster.save"></span><dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.cluster.save.update_batches">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.save.</span></span><span class="sig-name descname"><span class="pre">update_batches</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">dataset</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.data.ClusterMutsDataset" title="seismicrna.cluster.data.ClusterMutsDataset"><span class="pre">seismicrna.cluster.data.ClusterMutsDataset</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">orders</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.save.update_batches" title="Permalink to this definition"></a></dt>
+<dd><p>Update the cluster memberships in batches.</p>
+</dd></dl>
+
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.cluster.save.write_batches">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.save.</span></span><span class="sig-name descname"><span class="pre">write_batches</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">dataset</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="seismicrna.mask.html#seismicrna.mask.data.MaskMutsDataset" title="seismicrna.mask.data.MaskMutsDataset"><span class="pre">seismicrna.mask.data.MaskMutsDataset</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">orders</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.save.write_batches" title="Permalink to this definition"></a></dt>
+<dd><p>Write the cluster memberships to batch files.</p>
+</dd></dl>
+
 <span class="target" id="module-seismicrna.cluster.uniq"></span><dl class="py class">
 <dt class="sig sig-object py" id="seismicrna.cluster.uniq.UniqReads">
 <em class="property"><span class="pre">class</span><span class="w"> </span></em><span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.uniq.</span></span><span class="sig-name descname"><span class="pre">UniqReads</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">sample</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">section</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="seismicrna.core.seq.html#seismicrna.core.seq.section.Section" title="seismicrna.core.seq.section.Section"><span class="pre">seismicrna.core.seq.section.Section</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_mut_gap</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">muts_per_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><span class="pre">numpy.ndarray</span><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">batch_to_uniq</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><span class="pre">pandas.core.series.Series</span><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">counts_per_uniq</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.uniq.UniqReads" title="Permalink to this definition"></a></dt>
@@ -675,16 +687,47 @@ <h3>Cluster – Names Module<a class="headerlink" href="#cluster-names-module" t
 mutated at the position.</p>
 </dd></dl>
 
+<span class="target" id="module-seismicrna.cluster.update"></span><dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.cluster.update.add_orders">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.update.</span></span><span class="sig-name descname"><span class="pre">add_orders</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">cluster_report_file</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_order</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">temp_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">keep_temp</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">n_procs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.update.add_orders" title="Permalink to this definition"></a></dt>
+<dd><p>Add orders to an existing report and dataset.</p>
+</dd></dl>
+
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.cluster.update.run">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.update.</span></span><span class="sig-name descname"><span class="pre">run</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">input_path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_clusters</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">temp_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'./temp'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">keep_temp</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">10</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_procs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">16</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">parallel</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em><span class="sig-paren">)</span> <span class="sig-return"><span class="sig-return-icon">&#x2192;</span> <span class="sig-return-typehint"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a><span class="p"><span class="pre">]</span></span></span></span><a class="headerlink" href="#seismicrna.cluster.update.run" title="Permalink to this definition"></a></dt>
+<dd><p>Add more clusters to a dataset that was already clustered.</p>
+<dl class="field-list simple">
+<dt class="field-odd">Parameters</dt>
+<dd class="field-odd"><ul class="simple">
+<li><p><strong>max_clusters</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Maximum number of clusters to attempt (0 to disable) [keyword-only, default: 0]</p></li>
+<li><p><strong>temp_dir</strong> (<a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">str</span></code></a>) – Destination for all temporary files [keyword-only, default: ‘./temp’]</p></li>
+<li><p><strong>keep_temp</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">bool</span></code></a>) – Keep temporary files after the program exits [keyword-only, default: False]</p></li>
+<li><p><strong>brotli_level</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Compression level for brotli (0 - 11) [keyword-only, default: 10]</p></li>
+<li><p><strong>max_procs</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Maximum number of simultaneous processes [keyword-only, default: 16]</p></li>
+<li><p><strong>parallel</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">bool</span></code></a>) – Run tasks in parallel [keyword-only, default: True]</p></li>
+</ul>
+</dd>
+</dl>
+</dd></dl>
+
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.cluster.update.update_field">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.update.</span></span><span class="sig-name descname"><span class="pre">update_field</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">report</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.report.ClustReport" title="seismicrna.cluster.report.ClustReport"><span class="pre">seismicrna.cluster.report.ClustReport</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">field</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="seismicrna.core.html#seismicrna.core.report.Field" title="seismicrna.core.report.Field"><span class="pre">seismicrna.core.report.Field</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">orders</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#list" title="(in Python v3.12)"><span class="pre">list</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">attr</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.update.update_field" title="Permalink to this definition"></a></dt>
+<dd><p>Merge the field from the original report with the field from the
+new orders.</p>
+</dd></dl>
+
 <span class="target" id="module-seismicrna.cluster.write"></span><dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.cluster.write.cluster">
 <span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.write.</span></span><span class="sig-name descname"><span class="pre">cluster</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">mask_report_file</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_order</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">n_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">conv_thresh</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">n_procs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">force</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.write.cluster" title="Permalink to this definition"></a></dt>
-<dd><p>Run all processes of clustering reads from one filter.</p>
+<dd><p>Cluster unique reads from one mask dataset.</p>
 </dd></dl>
 
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.cluster.write.run_max_order">
 <span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.write.</span></span><span class="sig-name descname"><span class="pre">run_max_order</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">uniq_reads</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.uniq.UniqReads" title="seismicrna.cluster.uniq.UniqReads"><span class="pre">seismicrna.cluster.uniq.UniqReads</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_order</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">n_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">conv_thresh</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">n_procs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">top</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.write.run_max_order" title="Permalink to this definition"></a></dt>
-<dd><p>Find the optimal order (i.e. number of clusters) for EM, up to max_order.</p>
+<dd><p>Find the optimal order, from 1 to max_order.</p>
 </dd></dl>
 
 <dl class="py function">
@@ -694,9 +737,9 @@ <h3>Cluster – Names Module<a class="headerlink" href="#cluster-names-module" t
 </dd></dl>
 
 <dl class="py function">
-<dt class="sig sig-object py" id="seismicrna.cluster.write.write_batches">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.write.</span></span><span class="sig-name descname"><span class="pre">write_batches</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">dataset</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="seismicrna.mask.html#seismicrna.mask.data.MaskMutsDataset" title="seismicrna.mask.data.MaskMutsDataset"><span class="pre">seismicrna.mask.data.MaskMutsDataset</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">ord_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#dict" title="(in Python v3.12)"><span class="pre">dict</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="#seismicrna.cluster.compare.RunOrderResults" title="seismicrna.cluster.compare.RunOrderResults"><span class="pre">seismicrna.cluster.compare.RunOrderResults</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.write.write_batches" title="Permalink to this definition"></a></dt>
-<dd><p>Write the cluster memberships to batch files.</p>
+<dt class="sig sig-object py" id="seismicrna.cluster.write.run_orders">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.cluster.write.</span></span><span class="sig-name descname"><span class="pre">run_orders</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">uniq_reads</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.cluster.uniq.UniqReads" title="seismicrna.cluster.uniq.UniqReads"><span class="pre">seismicrna.cluster.uniq.UniqReads</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_order</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_order</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">n_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">prev_bic</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/constants.html#None" title="(in Python v3.12)"><span class="pre">None</span></a></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">conv_thresh</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">n_procs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">top</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.cluster.write.run_orders" title="Permalink to this definition"></a></dt>
+<dd><p>Find the optimal order, from min_order to max_order.</p>
 </dd></dl>
 
 </section>
diff --git a/docs/api/seismicrna.core.html b/docs/api/seismicrna.core.html
index 2ef46cfe..c5c93217 100644
--- a/docs/api/seismicrna.core.html
+++ b/docs/api/seismicrna.core.html
@@ -132,6 +132,13 @@ <h2>Subpackages<a class="headerlink" href="#subpackages" title="Permalink to thi
 </ul>
 </li>
 <li class="toctree-l1"><a class="reference internal" href="seismicrna.core.io.html">seismicrna.core.io package</a><ul>
+<li class="toctree-l2"><a class="reference internal" href="seismicrna.core.io.html#subpackages">Subpackages</a><ul>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.core.io.tests.html">seismicrna.core.io.tests package</a><ul>
+<li class="toctree-l4"><a class="reference internal" href="seismicrna.core.io.tests.html#module-seismicrna.core.io.tests.file_test">Submodules</a></li>
+</ul>
+</li>
+</ul>
+</li>
 <li class="toctree-l2"><a class="reference internal" href="seismicrna.core.io.html#module-seismicrna.core.io.batch">Submodules</a></li>
 </ul>
 </li>
@@ -1553,8 +1560,7 @@ <h3>Purpose<a class="headerlink" href="#purpose" title="Permalink to this headli
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.core.path.parse">
 <span class="sig-prename descclassname"><span class="pre">seismicrna.core.path.</span></span><span class="sig-name descname"><span class="pre">parse</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="o"><span class="pre">/</span></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span><span class="n"><span class="pre">segment_types</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.core.path.Segment" title="seismicrna.core.path.Segment"><span class="pre">seismicrna.core.path.Segment</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.path.parse" title="Permalink to this definition"></a></dt>
-<dd><p>Return the fields of a path given as a <cite>str</cite> based on the
-segment types.</p>
+<dd><p>Return the fields of a path based on the segment types.</p>
 </dd></dl>
 
 <dl class="py function">
@@ -1608,10 +1614,10 @@ <h3>Purpose<a class="headerlink" href="#purpose" title="Permalink to this headli
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.core.path.transpath">
 <span class="sig-prename descclassname"><span class="pre">seismicrna.core.path.</span></span><span class="sig-name descname"><span class="pre">transpath</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">to_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">from_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">strict</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.path.transpath" title="Permalink to this definition"></a></dt>
-<dd><p>Return the path that would result by moving <cite>path</cite> from <cite>indir</cite>
-to <cite>outdir</cite> (but do not actually move the path on the file system).
-This function does not require that any of the given paths exist
-unless <cite>strict</cite> is True.</p>
+<dd><p>Return the path that would be produced by moving <cite>path</cite> from
+<cite>from_dir</cite> to <cite>to_dir</cite> (but do not actually move the path on the
+file system). This function does not require that any of the given
+paths exist, unless <cite>strict</cite> is True.</p>
 <dl class="field-list simple">
 <dt class="field-odd">Parameters</dt>
 <dd class="field-odd"><ul class="simple">
@@ -1634,10 +1640,10 @@ <h3>Purpose<a class="headerlink" href="#purpose" title="Permalink to this headli
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.core.path.transpaths">
 <span class="sig-prename descclassname"><span class="pre">seismicrna.core.path.</span></span><span class="sig-name descname"><span class="pre">transpaths</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">to_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="o"><span class="pre">*</span></span><span class="n"><span class="pre">paths</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">strict</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.path.transpaths" title="Permalink to this definition"></a></dt>
-<dd><p>Return all paths that would result by moving the paths in <cite>path</cite>
-from their longest common sub-path to <cite>outdir</cite> (but do not actually
-move the paths on the file system). This function does not require
-that any of the given paths exist unless <cite>strict</cite> is True.</p>
+<dd><p>Return all paths that would be produced by moving all paths in
+<cite>paths</cite> from their longest common sub-path to <cite>to_dir</cite> (but do not
+actually move the paths on the file system). This function does not
+require that any of the given paths exist, unless <cite>strict</cite> is True.</p>
 <dl class="field-list simple">
 <dt class="field-odd">Parameters</dt>
 <dd class="field-odd"><ul class="simple">
@@ -1849,6 +1855,12 @@ <h3>Purpose<a class="headerlink" href="#purpose" title="Permalink to this headli
 
 </dd></dl>
 
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.core.report.calc_dt_minutes">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.core.report.</span></span><span class="sig-name descname"><span class="pre">calc_dt_minutes</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">began</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/datetime.html#datetime.datetime" title="(in Python v3.12)"><span class="pre">datetime.datetime</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">ended</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/datetime.html#datetime.datetime" title="(in Python v3.12)"><span class="pre">datetime.datetime</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.report.calc_dt_minutes" title="Permalink to this definition"></a></dt>
+<dd><p>Calculate the time taken in minutes.</p>
+</dd></dl>
+
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.core.report.calc_taken">
 <span class="sig-prename descclassname"><span class="pre">seismicrna.core.report.</span></span><span class="sig-name descname"><span class="pre">calc_taken</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">report</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="#seismicrna.core.report.Report" title="seismicrna.core.report.Report"><span class="pre">seismicrna.core.report.Report</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.report.calc_taken" title="Permalink to this definition"></a></dt>
@@ -2011,12 +2023,12 @@ <h3>Purpose<a class="headerlink" href="#purpose" title="Permalink to this headli
 <span class="target" id="module-seismicrna.core.version"></span><p>Version information for SEISMIC-RNA</p>
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.core.version.format_version">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.core.version.</span></span><span class="sig-name descname"><span class="pre">format_version</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">major</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">minor</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">11</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">patch</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">4</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.version.format_version" title="Permalink to this definition"></a></dt>
+<span class="sig-prename descclassname"><span class="pre">seismicrna.core.version.</span></span><span class="sig-name descname"><span class="pre">format_version</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">major</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">minor</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">12</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">patch</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">1</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.version.format_version" title="Permalink to this definition"></a></dt>
 <dd></dd></dl>
 
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.core.version.parse_version">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.core.version.</span></span><span class="sig-name descname"><span class="pre">parse_version</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">version</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'0.11.4'</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.version.parse_version" title="Permalink to this definition"></a></dt>
+<span class="sig-prename descclassname"><span class="pre">seismicrna.core.version.</span></span><span class="sig-name descname"><span class="pre">parse_version</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">version</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'0.12.1'</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.version.parse_version" title="Permalink to this definition"></a></dt>
 <dd></dd></dl>
 
 <span class="target" id="module-seismicrna.core.write"></span><dl class="py function">
diff --git a/docs/api/seismicrna.core.io.html b/docs/api/seismicrna.core.io.html
index eb533309..19ac99ec 100644
--- a/docs/api/seismicrna.core.io.html
+++ b/docs/api/seismicrna.core.io.html
@@ -19,7 +19,7 @@
     <script src="../_static/js/theme.js"></script>
     <link rel="index" title="Index" href="../genindex.html" />
     <link rel="search" title="Search" href="../search.html" />
-    <link rel="next" title="seismicrna.core.mu package" href="seismicrna.core.mu.html" />
+    <link rel="next" title="seismicrna.core.io.tests package" href="seismicrna.core.io.tests.html" />
     <link rel="prev" title="seismicrna.core.extern package" href="seismicrna.core.extern.html" /> 
 </head>
 
@@ -113,6 +113,17 @@
              
   <section id="module-seismicrna.core.io">
 <span id="seismicrna-core-io-package"></span><h1>seismicrna.core.io package<a class="headerlink" href="#module-seismicrna.core.io" title="Permalink to this headline"></a></h1>
+<section id="subpackages">
+<h2>Subpackages<a class="headerlink" href="#subpackages" title="Permalink to this headline"></a></h2>
+<div class="toctree-wrapper compound">
+<ul>
+<li class="toctree-l1"><a class="reference internal" href="seismicrna.core.io.tests.html">seismicrna.core.io.tests package</a><ul>
+<li class="toctree-l2"><a class="reference internal" href="seismicrna.core.io.tests.html#module-seismicrna.core.io.tests.file_test">Submodules</a></li>
+</ul>
+</li>
+</ul>
+</div>
+</section>
 <section id="module-seismicrna.core.io.batch">
 <span id="submodules"></span><h2>Submodules<a class="headerlink" href="#module-seismicrna.core.io.batch" title="Permalink to this headline"></a></h2>
 <dl class="py class">
@@ -269,6 +280,12 @@
 
 </dd></dl>
 
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.core.io.file.make_temp_backup">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.core.io.file.</span></span><span class="sig-name descname"><span class="pre">make_temp_backup</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">source_path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">out_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">temp_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.file.make_temp_backup" title="Permalink to this definition"></a></dt>
+<dd><p>Make a temporary backup of <cite>source_path</cite> in <cite>temp_dir</cite>.</p>
+</dd></dl>
+
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.core.io.file.recast_file_path">
 <span class="sig-prename descclassname"><span class="pre">seismicrna.core.io.file.</span></span><span class="sig-name descname"><span class="pre">recast_file_path</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">input_path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">input_type</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#type" title="(in Python v3.12)"><span class="pre">type</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.core.io.file.FileIO" title="seismicrna.core.io.file.FileIO"><span class="pre">seismicrna.core.io.file.FileIO</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">output_type</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#type" title="(in Python v3.12)"><span class="pre">type</span></a><span class="p"><span class="pre">[</span></span><a class="reference internal" href="#seismicrna.core.io.file.FileIO" title="seismicrna.core.io.file.FileIO"><span class="pre">seismicrna.core.io.file.FileIO</span></a><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="o"><span class="pre">**</span></span><span class="n"><span class="pre">override</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/typing.html#typing.Any" title="(in Python v3.12)"><span class="pre">Any</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.file.recast_file_path" title="Permalink to this definition"></a></dt>
@@ -293,6 +310,12 @@
 </dl>
 </dd></dl>
 
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.core.io.file.restore_temp_backup">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.core.io.file.</span></span><span class="sig-name descname"><span class="pre">restore_temp_backup</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">source_path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">out_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">temp_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.file.restore_temp_backup" title="Permalink to this definition"></a></dt>
+<dd><p>Restore the original files from a temporary backup.</p>
+</dd></dl>
+
 <span class="target" id="module-seismicrna.core.io.seq"></span><dl class="py class">
 <dt class="sig sig-object py" id="seismicrna.core.io.seq.RefseqIO">
 <em class="property"><span class="pre">class</span><span class="w"> </span></em><span class="sig-prename descclassname"><span class="pre">seismicrna.core.io.seq.</span></span><span class="sig-name descname"><span class="pre">RefseqIO</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="o"><span class="pre">*</span></span><span class="n"><span class="pre">args</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">refseq</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference internal" href="seismicrna.core.seq.html#seismicrna.core.seq.xna.DNA" title="seismicrna.core.seq.xna.DNA"><span class="pre">seismicrna.core.seq.xna.DNA</span></a></span></em>, <em class="sig-param"><span class="o"><span class="pre">**</span></span><span class="n"><span class="pre">kwargs</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.seq.RefseqIO" title="Permalink to this definition"></a></dt>
@@ -324,7 +347,7 @@
           </div>
           <footer><div class="rst-footer-buttons" role="navigation" aria-label="Footer">
         <a href="seismicrna.core.extern.html" class="btn btn-neutral float-left" title="seismicrna.core.extern package" accesskey="p" rel="prev"><span class="fa fa-arrow-circle-left" aria-hidden="true"></span> Previous</a>
-        <a href="seismicrna.core.mu.html" class="btn btn-neutral float-right" title="seismicrna.core.mu package" accesskey="n" rel="next">Next <span class="fa fa-arrow-circle-right" aria-hidden="true"></span></a>
+        <a href="seismicrna.core.io.tests.html" class="btn btn-neutral float-right" title="seismicrna.core.io.tests package" accesskey="n" rel="next">Next <span class="fa fa-arrow-circle-right" aria-hidden="true"></span></a>
     </div>
 
   <hr/>
diff --git a/docs/api/seismicrna.core.io.tests.html b/docs/api/seismicrna.core.io.tests.html
new file mode 100644
index 00000000..d69e143b
--- /dev/null
+++ b/docs/api/seismicrna.core.io.tests.html
@@ -0,0 +1,205 @@
+<!DOCTYPE html>
+<html class="writer-html5" lang="en" >
+<head>
+  <meta charset="utf-8" /><meta name="generator" content="Docutils 0.17.1: http://docutils.sourceforge.net/" />
+
+  <meta name="viewport" content="width=device-width, initial-scale=1.0" />
+  <title>seismicrna.core.io.tests package &mdash; seismic-rna  documentation</title>
+      <link rel="stylesheet" href="../_static/pygments.css" type="text/css" />
+      <link rel="stylesheet" href="../_static/css/theme.css" type="text/css" />
+    <link rel="shortcut icon" href="../_static/favicon-32x32.ico"/>
+  <!--[if lt IE 9]>
+    <script src="../_static/js/html5shiv.min.js"></script>
+  <![endif]-->
+  
+        <script data-url_root="../" id="documentation_options" src="../_static/documentation_options.js"></script>
+        <script src="../_static/jquery.js"></script>
+        <script src="../_static/underscore.js"></script>
+        <script src="../_static/doctools.js"></script>
+    <script src="../_static/js/theme.js"></script>
+    <link rel="index" title="Index" href="../genindex.html" />
+    <link rel="search" title="Search" href="../search.html" />
+    <link rel="next" title="seismicrna.core.mu package" href="seismicrna.core.mu.html" />
+    <link rel="prev" title="seismicrna.core.io package" href="seismicrna.core.io.html" /> 
+</head>
+
+<body class="wy-body-for-nav"> 
+  <div class="wy-grid-for-nav">
+    <nav data-toggle="wy-nav-shift" class="wy-nav-side">
+      <div class="wy-side-scroll">
+        <div class="wy-side-nav-search" >
+
+          
+          
+          <a href="../index.html" class="icon icon-home">
+            seismic-rna
+              <img src="../_static/logo-200.png" class="logo" alt="Logo"/>
+          </a>
+<div role="search">
+  <form id="rtd-search-form" class="wy-form" action="../search.html" method="get">
+    <input type="text" name="q" placeholder="Search docs" aria-label="Search docs" />
+    <input type="hidden" name="check_keywords" value="yes" />
+    <input type="hidden" name="area" value="default" />
+  </form>
+</div>
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+<li class="toctree-l2"><a class="reference internal" href="seismicrna.html#seismic-rna">SEISMIC-RNA</a></li>
+<li class="toctree-l2 current"><a class="reference internal" href="seismicrna.html#subpackages">Subpackages</a><ul class="current">
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+<li class="toctree-l3"><a class="reference internal" href="seismicrna.cleanfa.html">seismicrna.cleanfa package</a></li>
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+<li class="toctree-l3 current"><a class="reference internal" href="seismicrna.core.html">seismicrna.core package</a><ul class="current">
+<li class="toctree-l4 current"><a class="reference internal" href="seismicrna.core.html#subpackages">Subpackages</a></li>
+<li class="toctree-l4"><a class="reference internal" href="seismicrna.core.html#module-seismicrna.core.data">Submodules</a></li>
+</ul>
+</li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.demult.html">seismicrna.demult package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.export.html">seismicrna.export package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.fold.html">seismicrna.fold package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.graph.html">seismicrna.graph package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.mask.html">seismicrna.mask package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.pool.html">seismicrna.pool package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.relate.html">seismicrna.relate package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.renumct.html">seismicrna.renumct package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.sim.html">seismicrna.sim package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.table.html">seismicrna.table package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.test.html">seismicrna.test package</a></li>
+<li class="toctree-l3"><a class="reference internal" href="seismicrna.wf.html">seismicrna.wf package</a></li>
+</ul>
+</li>
+<li class="toctree-l2"><a class="reference internal" href="seismicrna.html#module-seismicrna.main">Submodules</a></li>
+</ul>
+</li>
+<li class="toctree-l1"><a class="reference internal" href="../formats/index.html">File Formats</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../data/index.html">Data Structures</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../algos/index.html">Algorithms</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../issues.html">Bugs and Requests</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../writeme.html">How to Write this Manual</a></li>
+</ul>
+
+        </div>
+      </div>
+    </nav>
+
+    <section data-toggle="wy-nav-shift" class="wy-nav-content-wrap"><nav class="wy-nav-top" aria-label="Mobile navigation menu" >
+          <i data-toggle="wy-nav-top" class="fa fa-bars"></i>
+          <a href="../index.html">seismic-rna</a>
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+  <ul class="wy-breadcrumbs">
+      <li><a href="../index.html" class="icon icon-home" aria-label="Home"></a></li>
+          <li class="breadcrumb-item"><a href="seismicrna.html">seismicrna package</a></li>
+          <li class="breadcrumb-item"><a href="seismicrna.core.html">seismicrna.core package</a></li>
+          <li class="breadcrumb-item"><a href="seismicrna.core.io.html">seismicrna.core.io package</a></li>
+      <li class="breadcrumb-item active">seismicrna.core.io.tests package</li>
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+            <a href="../_sources/api/seismicrna.core.io.tests.rst.txt" rel="nofollow"> View page source</a>
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+  </ul>
+  <hr/>
+</div>
+          <div role="main" class="document" itemscope="itemscope" itemtype="http://schema.org/Article">
+           <div itemprop="articleBody">
+             
+  <section id="module-seismicrna.core.io.tests">
+<span id="seismicrna-core-io-tests-package"></span><h1>seismicrna.core.io.tests package<a class="headerlink" href="#module-seismicrna.core.io.tests" title="Permalink to this headline"></a></h1>
+<section id="module-seismicrna.core.io.tests.file_test">
+<span id="submodules"></span><h2>Submodules<a class="headerlink" href="#module-seismicrna.core.io.tests.file_test" title="Permalink to this headline"></a></h2>
+<dl class="py class">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.SourceBackupDirs">
+<em class="property"><span class="pre">class</span><span class="w"> </span></em><span class="sig-prename descclassname"><span class="pre">seismicrna.core.io.tests.file_test.</span></span><span class="sig-name descname"><span class="pre">SourceBackupDirs</span></span><a class="headerlink" href="#seismicrna.core.io.tests.file_test.SourceBackupDirs" title="Permalink to this definition"></a></dt>
+<dd><p>Bases: <a class="reference external" href="https://docs.python.org/3/library/functions.html#object" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">object</span></code></a></p>
+<dl class="py property">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.SourceBackupDirs.backup_dir">
+<em class="property"><span class="pre">property</span><span class="w"> </span></em><span class="sig-name descname"><span class="pre">backup_dir</span></span><em class="property"><span class="p"><span class="pre">:</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></em><a class="headerlink" href="#seismicrna.core.io.tests.file_test.SourceBackupDirs.backup_dir" title="Permalink to this definition"></a></dt>
+<dd></dd></dl>
+
+<dl class="py property">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.SourceBackupDirs.source_dir">
+<em class="property"><span class="pre">property</span><span class="w"> </span></em><span class="sig-name descname"><span class="pre">source_dir</span></span><em class="property"><span class="p"><span class="pre">:</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></em><a class="headerlink" href="#seismicrna.core.io.tests.file_test.SourceBackupDirs.source_dir" title="Permalink to this definition"></a></dt>
+<dd></dd></dl>
+
+</dd></dl>
+
+<dl class="py class">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.TestMakeTempBackup">
+<em class="property"><span class="pre">class</span><span class="w"> </span></em><span class="sig-prename descclassname"><span class="pre">seismicrna.core.io.tests.file_test.</span></span><span class="sig-name descname"><span class="pre">TestMakeTempBackup</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">methodName</span></span><span class="o"><span class="pre">=</span></span><span class="default_value"><span class="pre">'runTest'</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.tests.file_test.TestMakeTempBackup" title="Permalink to this definition"></a></dt>
+<dd><p>Bases: <code class="xref py py-class docutils literal notranslate"><span class="pre">unittest.case.TestCase</span></code></p>
+<dl class="py method">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.TestMakeTempBackup.make_dir">
+<em class="property"><span class="pre">static</span><span class="w"> </span></em><span class="sig-name descname"><span class="pre">make_dir</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">in_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.tests.file_test.TestMakeTempBackup.make_dir" title="Permalink to this definition"></a></dt>
+<dd></dd></dl>
+
+<dl class="py method">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.TestMakeTempBackup.make_file">
+<em class="property"><span class="pre">static</span><span class="w"> </span></em><span class="sig-name descname"><span class="pre">make_file</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">in_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/pathlib.html#pathlib.Path" title="(in Python v3.12)"><span class="pre">pathlib.Path</span></a></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.tests.file_test.TestMakeTempBackup.make_file" title="Permalink to this definition"></a></dt>
+<dd></dd></dl>
+
+<dl class="py method">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_backup_dir">
+<span class="sig-name descname"><span class="pre">test_backup_dir</span></span><span class="sig-paren">(</span><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_backup_dir" title="Permalink to this definition"></a></dt>
+<dd></dd></dl>
+
+<dl class="py method">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_backup_file">
+<span class="sig-name descname"><span class="pre">test_backup_file</span></span><span class="sig-paren">(</span><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_backup_file" title="Permalink to this definition"></a></dt>
+<dd></dd></dl>
+
+<dl class="py method">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_restore_dir">
+<span class="sig-name descname"><span class="pre">test_restore_dir</span></span><span class="sig-paren">(</span><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_restore_dir" title="Permalink to this definition"></a></dt>
+<dd></dd></dl>
+
+<dl class="py method">
+<dt class="sig sig-object py" id="seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_restore_file">
+<span class="sig-name descname"><span class="pre">test_restore_file</span></span><span class="sig-paren">(</span><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_restore_file" title="Permalink to this definition"></a></dt>
+<dd></dd></dl>
+
+</dd></dl>
+
+</section>
+</section>
+
+
+           </div>
+          </div>
+          <footer><div class="rst-footer-buttons" role="navigation" aria-label="Footer">
+        <a href="seismicrna.core.io.html" class="btn btn-neutral float-left" title="seismicrna.core.io package" accesskey="p" rel="prev"><span class="fa fa-arrow-circle-left" aria-hidden="true"></span> Previous</a>
+        <a href="seismicrna.core.mu.html" class="btn btn-neutral float-right" title="seismicrna.core.mu package" accesskey="n" rel="next">Next <span class="fa fa-arrow-circle-right" aria-hidden="true"></span></a>
+    </div>
+
+  <hr/>
+
+  <div role="contentinfo">
+    <p>&#169; Copyright 2024, the Rouskin Lab.</p>
+  </div>
+
+  Built with <a href="https://www.sphinx-doc.org/">Sphinx</a> using a
+    <a href="https://github.com/readthedocs/sphinx_rtd_theme">theme</a>
+    provided by <a href="https://readthedocs.org">Read the Docs</a>.
+   
+
+</footer>
+        </div>
+      </div>
+    </section>
+  </div>
+  <script>
+      jQuery(function () {
+          SphinxRtdTheme.Navigation.enable(true);
+      });
+  </script> 
+
+</body>
+</html>
\ No newline at end of file
diff --git a/docs/api/seismicrna.core.mu.html b/docs/api/seismicrna.core.mu.html
index 2a48ba10..5265a76e 100644
--- a/docs/api/seismicrna.core.mu.html
+++ b/docs/api/seismicrna.core.mu.html
@@ -20,7 +20,7 @@
     <link rel="index" title="Index" href="../genindex.html" />
     <link rel="search" title="Search" href="../search.html" />
     <link rel="next" title="seismicrna.core.mu.tests package" href="seismicrna.core.mu.tests.html" />
-    <link rel="prev" title="seismicrna.core.io package" href="seismicrna.core.io.html" /> 
+    <link rel="prev" title="seismicrna.core.io.tests package" href="seismicrna.core.io.tests.html" /> 
 </head>
 
 <body class="wy-body-for-nav"> 
@@ -164,8 +164,8 @@ <h2>Subpackages<a class="headerlink" href="#subpackages" title="Permalink to thi
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.core.mu.compare.calc_nrmsd">
 <span class="sig-prename descclassname"><span class="pre">seismicrna.core.mu.compare.</span></span><span class="sig-name descname"><span class="pre">calc_nrmsd</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">mus1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.series.Series</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.frame.DataFrame</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">mus2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.series.Series</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.frame.DataFrame</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.mu.compare.calc_nrmsd" title="Permalink to this definition"></a></dt>
-<dd><p>Calculate the root-mean-square deviation (NRMSD) between two
-groups of mutation rates, ignoring NaNs.</p>
+<dd><p>Calculate the normalized root-mean-square deviation (NRMSD) of
+two groups of mutation rates, ignoring NaNs.</p>
 <dl class="field-list simple">
 <dt class="field-odd">Parameters</dt>
 <dd class="field-odd"><ul class="simple">
@@ -176,7 +176,7 @@ <h2>Subpackages<a class="headerlink" href="#subpackages" title="Permalink to thi
 </ul>
 </dd>
 <dt class="field-even">Returns</dt>
-<dd class="field-even"><p>Standardized mutation rates.</p>
+<dd class="field-even"><p>Normalized root-mean-square deviation (NRMSD)</p>
 </dd>
 <dt class="field-odd">Return type</dt>
 <dd class="field-odd"><p><code class="xref py py-class docutils literal notranslate"><span class="pre">np.ndarray</span> <span class="pre">|</span> <span class="pre">pd.Series</span> <span class="pre">|</span> <span class="pre">pd.DataFrame</span></code></p>
@@ -207,6 +207,29 @@ <h2>Subpackages<a class="headerlink" href="#subpackages" title="Permalink to thi
 </dl>
 </dd></dl>
 
+<dl class="py function">
+<dt class="sig sig-object py" id="seismicrna.core.mu.compare.calc_rmsd">
+<span class="sig-prename descclassname"><span class="pre">seismicrna.core.mu.compare.</span></span><span class="sig-name descname"><span class="pre">calc_rmsd</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">mus1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.series.Series</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.frame.DataFrame</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">mus2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.series.Series</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.frame.DataFrame</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.mu.compare.calc_rmsd" title="Permalink to this definition"></a></dt>
+<dd><p>Calculate the root-mean-square deviation (RMSD) of two groups of
+mutation rates, ignoring NaNs.</p>
+<dl class="field-list simple">
+<dt class="field-odd">Parameters</dt>
+<dd class="field-odd"><ul class="simple">
+<li><p><strong>mus1</strong> (<code class="xref py py-class docutils literal notranslate"><span class="pre">np.ndarray</span> <span class="pre">|</span> <span class="pre">pd.Series</span> <span class="pre">|</span> <span class="pre">pd.DataFrame</span></code>) – First group of mutation rates; can contain multiple sets as the
+columns of a multidimensional array or DataFrame.</p></li>
+<li><p><strong>mus2</strong> (<code class="xref py py-class docutils literal notranslate"><span class="pre">np.ndarray</span> <span class="pre">|</span> <span class="pre">pd.Series</span> <span class="pre">|</span> <span class="pre">pd.DataFrame</span></code>) – Second group of mutation rates; can contain multiple sets as the
+columns of a multidimensional array or DataFrame.</p></li>
+</ul>
+</dd>
+<dt class="field-even">Returns</dt>
+<dd class="field-even"><p>Root-mean-square deviation (RMSD)</p>
+</dd>
+<dt class="field-odd">Return type</dt>
+<dd class="field-odd"><p><code class="xref py py-class docutils literal notranslate"><span class="pre">np.ndarray</span> <span class="pre">|</span> <span class="pre">pd.Series</span> <span class="pre">|</span> <span class="pre">pd.DataFrame</span></code></p>
+</dd>
+</dl>
+</dd></dl>
+
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.core.mu.compare.calc_spearman">
 <span class="sig-prename descclassname"><span class="pre">seismicrna.core.mu.compare.</span></span><span class="sig-name descname"><span class="pre">calc_spearman</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="n"><span class="pre">mus1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.series.Series</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.frame.DataFrame</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">mus2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><span class="pre">numpy.ndarray</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.series.Series</span><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><span class="pre">pandas.core.frame.DataFrame</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.core.mu.compare.calc_spearman" title="Permalink to this definition"></a></dt>
@@ -634,7 +657,7 @@ <h2>Subpackages<a class="headerlink" href="#subpackages" title="Permalink to thi
            </div>
           </div>
           <footer><div class="rst-footer-buttons" role="navigation" aria-label="Footer">
-        <a href="seismicrna.core.io.html" class="btn btn-neutral float-left" title="seismicrna.core.io package" accesskey="p" rel="prev"><span class="fa fa-arrow-circle-left" aria-hidden="true"></span> Previous</a>
+        <a href="seismicrna.core.io.tests.html" class="btn btn-neutral float-left" title="seismicrna.core.io.tests package" accesskey="p" rel="prev"><span class="fa fa-arrow-circle-left" aria-hidden="true"></span> Previous</a>
         <a href="seismicrna.core.mu.tests.html" class="btn btn-neutral float-right" title="seismicrna.core.mu.tests package" accesskey="n" rel="next">Next <span class="fa fa-arrow-circle-right" aria-hidden="true"></span></a>
     </div>
 
diff --git a/docs/api/seismicrna.html b/docs/api/seismicrna.html
index 2953cc41..cee78145 100644
--- a/docs/api/seismicrna.html
+++ b/docs/api/seismicrna.html
@@ -171,6 +171,7 @@ <h2>Subpackages<a class="headerlink" href="#subpackages" title="Permalink to thi
 </ul>
 </li>
 <li class="toctree-l3"><a class="reference internal" href="seismicrna.core.io.html">seismicrna.core.io package</a><ul>
+<li class="toctree-l4"><a class="reference internal" href="seismicrna.core.io.html#subpackages">Subpackages</a></li>
 <li class="toctree-l4"><a class="reference internal" href="seismicrna.core.io.html#module-seismicrna.core.io.batch">Submodules</a></li>
 </ul>
 </li>
diff --git a/docs/api/seismicrna.wf.html b/docs/api/seismicrna.wf.html
index 5b21536a..acb29b4d 100644
--- a/docs/api/seismicrna.wf.html
+++ b/docs/api/seismicrna.wf.html
@@ -122,7 +122,7 @@ <h3>Whole Pipeline Main Module<a class="headerlink" href="#whole-pipeline-main-m
 
 <dl class="py function">
 <dt class="sig sig-object py" id="seismicrna.wf.main.run">
-<span class="sig-prename descclassname"><span class="pre">seismicrna.wf.main.</span></span><span class="sig-name descname"><span class="pre">run</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">input_path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">out_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'./out'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">temp_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'./temp'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">keep_temp</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">10</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">force</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_procs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">16</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">parallel</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fasta</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">fastqz</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fastqy</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fastqx</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">phred_enc</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">33</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">demulti_overwrite</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">demult_on</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">parallel_demultiplexing</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">clipped</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">mismatch_tolerence</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">index_tolerance</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">barcode_start</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">barcode_end</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">dmfastqz</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">dmfastqy</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">dmfastqx</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fastqc</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">qc_extract</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_q1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">25</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_q2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">25</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_g1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">('GCTCTTCCGATCT',)</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_a1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">('AGATCGGAAGAGC',)</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_g2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">('GCTCTTCCGATCT',)</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_a2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">('AGATCGGAAGAGC',)</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_o</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">6</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_e</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.1</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_indels</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_nextseq</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_discard_trimmed</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_discard_untrimmed</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_m</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">20</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_local</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_discordant</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_mixed</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_dovetail</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_contain</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_score_min_e2e</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'L,-1,-0.5'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_score_min_loc</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'L,1,0.5'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_i</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_x</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">600</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_gbar</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">4</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_l</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">20</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_s</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'L,1,0.1'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_d</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">4</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_r</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">2</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_dpad</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">2</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_orient</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'fr'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_un</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_mapq</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">25</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cram</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_phred</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">25</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_reads</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">1000</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">ambrel</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">batch_size</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">64.0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">pool</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">''</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">coords</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">]</span></span><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">primers</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="seismicrna.core.seq.html#seismicrna.core.seq.xna.DNA" title="seismicrna.core.seq.xna.DNA"><span class="pre">seismicrna.core.seq.xna.DNA</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="seismicrna.core.seq.html#seismicrna.core.seq.xna.DNA" title="seismicrna.core.seq.xna.DNA"><span class="pre">seismicrna.core.seq.xna.DNA</span></a><span class="p"><span class="pre">]</span></span><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">primer_gap</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">sections_file</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">''</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">count_del</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">count_ins</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">discount_mut</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">exclude_polya</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">5</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">exclude_gu</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">exclude_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">]</span></span><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_finfo_read</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_fmut_read</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.1</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_mut_gap</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_ninfo_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">1000</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_fmut_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.5</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_clusters</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">em_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">6</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_em_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">8</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_em_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">512</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">em_thresh</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.01</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">table_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">table_read</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">table_clust</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">quantile</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_temp</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">310.15</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_constraint</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/constants.html#None" title="(in Python v3.12)"><span class="pre">None</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_md</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_mfe</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_max</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">20</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_percent</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">20.0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">export</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">samples_meta</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">''</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">refs_meta</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">''</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">all_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cgroup</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'order'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">hist_bins</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">10</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">hist_margin</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.01</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">window</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">45</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">winmin</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">9</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">csv</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">html</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">pdf</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.wf.main.run" title="Permalink to this definition"></a></dt>
+<span class="sig-prename descclassname"><span class="pre">seismicrna.wf.main.</span></span><span class="sig-name descname"><span class="pre">run</span></span><span class="sig-paren">(</span><em class="sig-param"><span class="o"><span class="pre">*</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">input_path</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span></em>, <em class="sig-param"><span class="n"><span class="pre">out_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'./out'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">temp_dir</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'./temp'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">keep_temp</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">brotli_level</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">10</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">force</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_procs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">16</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">parallel</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fasta</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">fastqz</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fastqy</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fastqx</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">phred_enc</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">33</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">demulti_overwrite</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">demult_on</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">parallel_demultiplexing</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">clipped</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">mismatch_tolerence</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">index_tolerance</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">barcode_start</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">barcode_end</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">dmfastqz</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">dmfastqy</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">dmfastqx</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fastqc</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">qc_extract</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_q1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">25</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_q2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">25</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_g1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">('GCTCTTCCGATCT',)</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_a1</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">('AGATCGGAAGAGC',)</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_g2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">('GCTCTTCCGATCT',)</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_a2</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">('AGATCGGAAGAGC',)</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_o</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">6</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_e</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.1</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_indels</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_nextseq</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_discard_trimmed</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_discard_untrimmed</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cut_m</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">20</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_local</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_discordant</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_mixed</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_dovetail</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_contain</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_score_min_e2e</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'L,-1,-0.5'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_score_min_loc</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'L,1,0.5'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_i</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_x</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">600</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_gbar</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">4</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_l</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">20</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_s</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'L,1,0.1'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_d</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">4</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_r</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">2</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_dpad</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">2</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_orient</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'fr'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">bt2_un</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_mapq</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">25</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cram</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_phred</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">25</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_reads</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">1000</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">ambrel</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">batch_size</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">64.0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">pool</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">''</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">coords</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">]</span></span><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">primers</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="seismicrna.core.seq.html#seismicrna.core.seq.xna.DNA" title="seismicrna.core.seq.xna.DNA"><span class="pre">seismicrna.core.seq.xna.DNA</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference internal" href="seismicrna.core.seq.html#seismicrna.core.seq.xna.DNA" title="seismicrna.core.seq.xna.DNA"><span class="pre">seismicrna.core.seq.xna.DNA</span></a><span class="p"><span class="pre">]</span></span><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">primer_gap</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">sections_file</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">''</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">count_del</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">count_ins</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">discount_mut</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">exclude_polya</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">5</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">exclude_gu</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">exclude_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#tuple" title="(in Python v3.12)"><span class="pre">tuple</span></a><span class="p"><span class="pre">[</span></span><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="p"><span class="pre">,</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a><span class="p"><span class="pre">]</span></span><span class="p"><span class="pre">,</span></span><span class="w"> </span><span class="p"><span class="pre">...</span></span><span class="p"><span class="pre">]</span></span></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">()</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_finfo_read</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_fmut_read</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.1</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_mut_gap</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_ninfo_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">1000</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_fmut_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.5</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_clusters</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">em_runs</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">6</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">min_em_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">8</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">max_em_iter</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">512</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">em_thresh</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.6931471805599453</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">table_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">table_read</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">table_clust</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">quantile</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_temp</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">310.15</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_constraint</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a><span class="w"> </span><span class="p"><span class="pre">|</span></span><span class="w"> </span><a class="reference external" href="https://docs.python.org/3/library/constants.html#None" title="(in Python v3.12)"><span class="pre">None</span></a></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_md</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_mfe</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_max</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">20</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">fold_percent</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">20.0</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">export</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">samples_meta</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">''</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">refs_meta</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">''</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">all_pos</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">cgroup</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/stdtypes.html#str" title="(in Python v3.12)"><span class="pre">str</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">'order'</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">hist_bins</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">10</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">hist_margin</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><span class="pre">float</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">0.01</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">window</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">45</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">winmin</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><span class="pre">int</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">9</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">csv</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">html</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">True</span></span></em>, <em class="sig-param"><span class="n"><span class="pre">pdf</span></span><span class="p"><span class="pre">:</span></span><span class="w"> </span><span class="n"><a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><span class="pre">bool</span></a></span><span class="w"> </span><span class="o"><span class="pre">=</span></span><span class="w"> </span><span class="default_value"><span class="pre">False</span></span></em><span class="sig-paren">)</span><a class="headerlink" href="#seismicrna.wf.main.run" title="Permalink to this definition"></a></dt>
 <dd><p>Run the entire workflow.</p>
 <dl class="field-list simple">
 <dt class="field-odd">Parameters</dt>
@@ -208,7 +208,7 @@ <h3>Whole Pipeline Main Module<a class="headerlink" href="#whole-pipeline-main-m
 <li><p><strong>em_runs</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Number of independent runs for each number of clusters [keyword-only, default: 6]</p></li>
 <li><p><strong>min_em_iter</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Minimum iterations per clustering run [keyword-only, default: 8]</p></li>
 <li><p><strong>max_em_iter</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#int" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">int</span></code></a>) – Maximum iterations per clustering run [keyword-only, default: 512]</p></li>
-<li><p><strong>em_thresh</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">float</span></code></a>) – Maximum change in log likelihood for convergence [keyword-only, default: 0.01]</p></li>
+<li><p><strong>em_thresh</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#float" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">float</span></code></a>) – Maximum change in log likelihood for convergence [keyword-only, default: 0.6931471805599453]</p></li>
 <li><p><strong>table_pos</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">bool</span></code></a>) – Tabulate per position [keyword-only, default: True]</p></li>
 <li><p><strong>table_read</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">bool</span></code></a>) – Tabulate per read [keyword-only, default: True]</p></li>
 <li><p><strong>table_clust</strong> (<a class="reference external" href="https://docs.python.org/3/library/functions.html#bool" title="(in Python v3.12)"><code class="xref py py-class docutils literal notranslate"><span class="pre">bool</span></code></a>) – Tabulate per cluster [keyword-only, default: True]</p></li>
diff --git a/docs/genindex.html b/docs/genindex.html
index a8c06bb5..d2ee242b 100644
--- a/docs/genindex.html
+++ b/docs/genindex.html
@@ -2158,6 +2158,8 @@ <h2 id="A">A</h2>
       <li><a href="api/seismicrna.core.seq.html#seismicrna.core.seq.refs.RefSeqs.add">add() (seismicrna.core.seq.refs.RefSeqs method)</a>
 </li>
       <li><a href="api/seismicrna.core.seq.html#seismicrna.core.seq.section.Section.add_mask">add_mask() (seismicrna.core.seq.section.Section method)</a>
+</li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.update.add_orders">add_orders() (in module seismicrna.cluster.update)</a>
 </li>
       <li><a href="api/seismicrna.table.html#seismicrna.table.calc.adjust_counts">adjust_counts() (in module seismicrna.table.calc)</a>
 </li>
@@ -2222,6 +2224,8 @@ <h2 id="A">A</h2>
 </li>
       </ul></li>
       <li><a href="api/seismicrna.relate.aux.tests.html#seismicrna.relate.aux.tests.infer_test.TestInferRead.assert_raise">assert_raise() (seismicrna.relate.aux.tests.infer_test.TestInferRead method)</a>
+</li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.assign_clusterings">assign_clusterings() (in module seismicrna.cluster.compare)</a>
 </li>
       <li><a href="api/seismicrna.core.rna.html#seismicrna.core.rna.state.RNAState.auc">auc (seismicrna.core.rna.state.RNAState property)</a>
 </li>
@@ -2269,6 +2273,8 @@ <h2 id="A">A</h2>
 <h2 id="B">B</h2>
 <table style="width: 100%" class="indextable genindextable"><tr>
   <td style="width: 33%; vertical-align: top;"><ul>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.SourceBackupDirs.backup_dir">backup_dir (seismicrna.core.io.tests.file_test.SourceBackupDirs property)</a>
+</li>
       <li><a href="api/seismicrna.core.html#seismicrna.core.data.Dataset.batch_nums">batch_nums (seismicrna.core.data.Dataset property)</a>
 </li>
       <li><a href="api/seismicrna.core.batch.html#seismicrna.core.batch.read.ReadBatch.batch_read_index">batch_read_index (seismicrna.core.batch.read.ReadBatch property)</a>
@@ -2281,8 +2287,12 @@ <h2 id="B">B</h2>
 </li>
       <li><a href="api/seismicrna.core.html#seismicrna.core.report.BatchedReport">BatchedReport (class in seismicrna.core.report)</a>
 </li>
-      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.em.EmClustering.bic">bic (seismicrna.cluster.em.EmClustering property)</a>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.RunOrderResults.bic">bic (seismicrna.cluster.compare.RunOrderResults property)</a>
+
+      <ul>
+        <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.em.EmClustering.bic">(seismicrna.cluster.em.EmClustering property)</a>
 </li>
+      </ul></li>
       <li><a href="api/seismicrna.core.html#seismicrna.core.logs.AnsiCode.BLUE">BLUE (seismicrna.core.logs.AnsiCode attribute)</a>
 </li>
       <li><a href="api/seismicrna.core.html#seismicrna.core.logs.AnsiCode.BOLD">BOLD (seismicrna.core.logs.AnsiCode attribute)</a>
@@ -2294,11 +2304,11 @@ <h2 id="B">B</h2>
       <li><a href="api/seismicrna.align.html#seismicrna.align.fqunit.FastqUnit.BOWTIE2_FLAGS">BOWTIE2_FLAGS (seismicrna.align.fqunit.FastqUnit attribute)</a>
 </li>
       <li><a href="api/seismicrna.align.html#seismicrna.align.fqunit.FastqUnit.bowtie2_inputs">bowtie2_inputs (seismicrna.align.fqunit.FastqUnit property)</a>
-</li>
-      <li><a href="api/seismicrna.core.io.html#seismicrna.core.io.file.BrickleIO">BrickleIO (class in seismicrna.core.io.file)</a>
 </li>
   </ul></td>
   <td style="width: 33%; vertical-align: top;"><ul>
+      <li><a href="api/seismicrna.core.io.html#seismicrna.core.io.file.BrickleIO">BrickleIO (class in seismicrna.core.io.file)</a>
+</li>
       <li><a href="api/seismicrna.core.mu.tests.html#seismicrna.core.mu.tests.frame_test.broadcast_regex">broadcast_regex() (in module seismicrna.core.mu.tests.frame_test)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.tests.html#seismicrna.core.mu.tests.frame_test.broadcastable">broadcastable() (in module seismicrna.core.mu.tests.frame_test)</a>
@@ -2340,22 +2350,26 @@ <h2 id="C">C</h2>
       <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.em.calc_bic">calc_bic() (in module seismicrna.cluster.em)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.html#seismicrna.core.mu.compare.calc_coeff_determ">calc_coeff_determ() (in module seismicrna.core.mu.compare)</a>
+</li>
+      <li><a href="api/seismicrna.core.html#seismicrna.core.report.calc_dt_minutes">calc_dt_minutes() (in module seismicrna.core.report)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.unbias.html#seismicrna.core.mu.unbias.frame.calc_f_obs_frame">calc_f_obs_frame() (in module seismicrna.core.mu.unbias.frame)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.unbias.html#seismicrna.core.mu.unbias.algo.calc_f_obs_numpy">calc_f_obs_numpy() (in module seismicrna.core.mu.unbias.algo)</a>
 </li>
-      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.calc_information_entropy">calc_information_entropy() (in module seismicrna.cluster.compare)</a>
-</li>
-      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.calc_mean_var_info">calc_mean_var_info() (in module seismicrna.cluster.compare)</a>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.calc_mean_pearson">calc_mean_pearson() (in module seismicrna.cluster.compare)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.unbias.html#seismicrna.core.mu.unbias.frame.calc_mu_adj_frame">calc_mu_adj_frame() (in module seismicrna.core.mu.unbias.frame)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.unbias.html#seismicrna.core.mu.unbias.algo.calc_mu_adj_numpy">calc_mu_adj_numpy() (in module seismicrna.core.mu.unbias.algo)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.html#seismicrna.core.mu.compare.calc_nrmsd">calc_nrmsd() (in module seismicrna.core.mu.compare)</a>
+</li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.calc_nrmsd_groups">calc_nrmsd_groups() (in module seismicrna.cluster.compare)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.html#seismicrna.core.mu.compare.calc_pearson">calc_pearson() (in module seismicrna.core.mu.compare)</a>
+</li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.calc_pearson_groups">calc_pearson_groups() (in module seismicrna.cluster.compare)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.unbias.html#seismicrna.core.mu.unbias.frame.calc_prop_adj_frame">calc_prop_adj_frame() (in module seismicrna.core.mu.unbias.frame)</a>
 </li>
@@ -2366,6 +2380,12 @@ <h2 id="C">C</h2>
       <li><a href="api/seismicrna.core.mu.html#seismicrna.core.mu.scale.calc_ranks">calc_ranks() (in module seismicrna.core.mu.scale)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.html#seismicrna.core.mu.scale.calc_rms">calc_rms() (in module seismicrna.core.mu.scale)</a>
+</li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.calc_rms_nrmsd">calc_rms_nrmsd() (in module seismicrna.cluster.compare)</a>
+</li>
+      <li><a href="api/seismicrna.core.mu.html#seismicrna.core.mu.compare.calc_rmsd">calc_rmsd() (in module seismicrna.core.mu.compare)</a>
+</li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.calc_rmsd_groups">calc_rmsd_groups() (in module seismicrna.cluster.compare)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.html#seismicrna.core.mu.compare.calc_spearman">calc_spearman() (in module seismicrna.core.mu.compare)</a>
 </li>
@@ -2379,10 +2399,6 @@ <h2 id="C">C</h2>
         <li><a href="api/seismicrna.core.mu.tests.html#seismicrna.core.mu.tests.compare_test.TestCalcSpearman.calc_true">(seismicrna.core.mu.tests.compare_test.TestCalcSpearman class method)</a>
 </li>
       </ul></li>
-      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.calc_var_info_pair">calc_var_info_pair() (in module seismicrna.cluster.compare)</a>
-</li>
-      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.calc_var_info_pqr">calc_var_info_pqr() (in module seismicrna.cluster.compare)</a>
-</li>
       <li><a href="api/seismicrna.core.html#seismicrna.core.path.cast_path">cast_path() (in module seismicrna.core.path)</a>
 </li>
       <li><a href="api/seismicrna.graph.html#seismicrna.graph.base.cgroup_table">cgroup_table() (in module seismicrna.graph.base)</a>
@@ -2462,11 +2478,11 @@ <h2 id="C">C</h2>
       <li><a href="api/seismicrna.table.html#seismicrna.table.base.ClustReadTable">ClustReadTable (class in seismicrna.table.base)</a>
 </li>
       <li><a href="api/seismicrna.table.html#seismicrna.table.load.ClustReadTableLoader">ClustReadTableLoader (class in seismicrna.table.load)</a>
-</li>
-      <li><a href="api/seismicrna.table.html#seismicrna.table.write.ClustReadTableWriter">ClustReadTableWriter (class in seismicrna.table.write)</a>
 </li>
   </ul></td>
   <td style="width: 33%; vertical-align: top;"><ul>
+      <li><a href="api/seismicrna.table.html#seismicrna.table.write.ClustReadTableWriter">ClustReadTableWriter (class in seismicrna.table.write)</a>
+</li>
       <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.report.ClustReport">ClustReport (class in seismicrna.cluster.report)</a>
 </li>
       <li><a href="api/seismicrna.core.html#seismicrna.core.header.Header.clusts">clusts (seismicrna.core.header.Header property)</a>
@@ -3876,6 +3892,10 @@ <h2 id="M">M</h2>
 <table style="width: 100%" class="indextable genindextable"><tr>
   <td style="width: 33%; vertical-align: top;"><ul>
       <li><a href="api/seismicrna.demult.html#seismicrna.demult.demultiplex.make_dict_from_fasta">make_dict_from_fasta() (in module seismicrna.demult.demultiplex)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.TestMakeTempBackup.make_dir">make_dir() (seismicrna.core.io.tests.file_test.TestMakeTempBackup static method)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.TestMakeTempBackup.make_file">make_file() (seismicrna.core.io.tests.file_test.TestMakeTempBackup static method)</a>
 </li>
       <li><a href="api/seismicrna.core.html#seismicrna.core.header.make_header">make_header() (in module seismicrna.core.header)</a>
 </li>
@@ -3890,6 +3910,8 @@ <h2 id="M">M</h2>
       <li><a href="api/seismicrna.demult.html#seismicrna.demult.demultiplex.make_sequence_objects_from_csv">make_sequence_objects_from_csv() (in module seismicrna.demult.demultiplex)</a>
 </li>
       <li><a href="api/seismicrna.core.parallel.tests.html#seismicrna.core.parallel.tests.temp_test.make_temp">make_temp() (in module seismicrna.core.parallel.tests.temp_test)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.html#seismicrna.core.io.file.make_temp_backup">make_temp_backup() (in module seismicrna.core.io.file)</a>
 </li>
       <li><a href="api/seismicrna.graph.html#seismicrna.graph.base.make_title_action_sample">make_title_action_sample() (in module seismicrna.graph.base)</a>
 </li>
@@ -4102,8 +4124,12 @@ <h2 id="M">M</h2>
         <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.names">seismicrna.cluster.names</a>
 </li>
         <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.report">seismicrna.cluster.report</a>
+</li>
+        <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.save">seismicrna.cluster.save</a>
 </li>
         <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.uniq">seismicrna.cluster.uniq</a>
+</li>
+        <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.update">seismicrna.cluster.update</a>
 </li>
         <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.write">seismicrna.cluster.write</a>
 </li>
@@ -4150,6 +4176,10 @@ <h2 id="M">M</h2>
         <li><a href="api/seismicrna.core.io.html#module-seismicrna.core.io.file">seismicrna.core.io.file</a>
 </li>
         <li><a href="api/seismicrna.core.io.html#module-seismicrna.core.io.seq">seismicrna.core.io.seq</a>
+</li>
+        <li><a href="api/seismicrna.core.io.tests.html#module-seismicrna.core.io.tests">seismicrna.core.io.tests</a>
+</li>
+        <li><a href="api/seismicrna.core.io.tests.html#module-seismicrna.core.io.tests.file_test">seismicrna.core.io.tests.file_test</a>
 </li>
         <li><a href="api/seismicrna.core.html#module-seismicrna.core.logs">seismicrna.core.logs</a>
 </li>
@@ -5088,12 +5118,12 @@ <h2 id="R">R</h2>
 </li>
       <li><a href="api/seismicrna.graph.html#seismicrna.graph.base.GraphBase.relationships">relationships (seismicrna.graph.base.GraphBase property)</a>
 </li>
-  </ul></td>
-  <td style="width: 33%; vertical-align: top;"><ul>
       <li><a href="api/seismicrna.relate.html#seismicrna.relate.write.RelationWriter">RelationWriter (class in seismicrna.relate.write)</a>
 </li>
       <li><a href="api/seismicrna.core.html#seismicrna.core.header.RelClustHeader">RelClustHeader (class in seismicrna.core.header)</a>
 </li>
+  </ul></td>
+  <td style="width: 33%; vertical-align: top;"><ul>
       <li><a href="api/seismicrna.graph.html#seismicrna.graph.color.RelColorMap">RelColorMap (class in seismicrna.graph.color)</a>
 </li>
       <li><a href="api/seismicrna.core.html#seismicrna.core.header.RelHeader">RelHeader (class in seismicrna.core.header)</a>
@@ -5165,6 +5195,8 @@ <h2 id="R">R</h2>
       <li><a href="api/seismicrna.relate.py.html#seismicrna.relate.py.ambrel.Indel.reset">reset() (seismicrna.relate.py.ambrel.Indel method)</a>
 </li>
       <li><a href="api/seismicrna.demult.html#seismicrna.demult.demultiplex.resolve_or_analyze_multigrepped_reads">resolve_or_analyze_multigrepped_reads() (in module seismicrna.demult.demultiplex)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.html#seismicrna.core.io.file.restore_temp_backup">restore_temp_backup() (in module seismicrna.core.io.file)</a>
 </li>
       <li><a href="api/seismicrna.fold.html#seismicrna.fold.rnastructure.retitle_ct_structures">retitle_ct_structures() (in module seismicrna.fold.rnastructure)</a>
 </li>
@@ -5238,6 +5270,8 @@ <h2 id="R">R</h2>
         <li><a href="api/seismicrna.cleanfa.html#seismicrna.cleanfa.main.run">(in module seismicrna.cleanfa.main)</a>
 </li>
         <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.main.run">(in module seismicrna.cluster.main)</a>
+</li>
+        <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.update.run">(in module seismicrna.cluster.update)</a>
 </li>
         <li><a href="api/seismicrna.demult.html#seismicrna.demult.main.run">(in module seismicrna.demult.main)</a>
 </li>
@@ -5281,6 +5315,8 @@ <h2 id="R">R</h2>
       <li><a href="api/seismicrna.demult.html#seismicrna.demult.demultiplex.run_multi_greps">run_multi_greps() (in module seismicrna.demult.demultiplex)</a>
 </li>
       <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.write.run_order">run_order() (in module seismicrna.cluster.write)</a>
+</li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.write.run_orders">run_orders() (in module seismicrna.cluster.write)</a>
 </li>
       <li><a href="api/seismicrna.demult.html#seismicrna.demult.demultiplex.run_seqkit_grep">run_seqkit_grep() (in module seismicrna.demult.demultiplex)</a>
 </li>
@@ -6849,6 +6885,13 @@ <h2 id="S">S</h2>
 
       <ul>
         <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.report">module</a>
+</li>
+      </ul></li>
+      <li>
+    seismicrna.cluster.save
+
+      <ul>
+        <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.save">module</a>
 </li>
       </ul></li>
       <li>
@@ -6856,6 +6899,13 @@ <h2 id="S">S</h2>
 
       <ul>
         <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.uniq">module</a>
+</li>
+      </ul></li>
+      <li>
+    seismicrna.cluster.update
+
+      <ul>
+        <li><a href="api/seismicrna.cluster.html#module-seismicrna.cluster.update">module</a>
 </li>
       </ul></li>
       <li>
@@ -7017,6 +7067,20 @@ <h2 id="S">S</h2>
 
       <ul>
         <li><a href="api/seismicrna.core.io.html#module-seismicrna.core.io.seq">module</a>
+</li>
+      </ul></li>
+      <li>
+    seismicrna.core.io.tests
+
+      <ul>
+        <li><a href="api/seismicrna.core.io.tests.html#module-seismicrna.core.io.tests">module</a>
+</li>
+      </ul></li>
+      <li>
+    seismicrna.core.io.tests.file_test
+
+      <ul>
+        <li><a href="api/seismicrna.core.io.tests.html#module-seismicrna.core.io.tests.file_test">module</a>
 </li>
       </ul></li>
       <li>
@@ -8107,6 +8171,10 @@ <h2 id="S">S</h2>
       <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.compare.sort_replicate_runs">sort_replicate_runs() (in module seismicrna.cluster.compare)</a>
 </li>
       <li><a href="api/seismicrna.core.ngs.html#seismicrna.core.ngs.xam.sort_xam_cmd">sort_xam_cmd() (in module seismicrna.core.ngs.xam)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.SourceBackupDirs.source_dir">source_dir (seismicrna.core.io.tests.file_test.SourceBackupDirs property)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.SourceBackupDirs">SourceBackupDirs (class in seismicrna.core.io.tests.file_test)</a>
 </li>
       <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.em.EmClustering.sparse_mus">sparse_mus (seismicrna.cluster.em.EmClustering property)</a>
 </li>
@@ -8405,6 +8473,10 @@ <h2 id="T">T</h2>
       <li><a href="api/seismicrna.core.mu.tests.html#seismicrna.core.mu.tests.scale_test.TestWinsorize.test_arrays">test_arrays() (seismicrna.core.mu.tests.scale_test.TestWinsorize method)</a>
 </li>
       <li><a href="api/seismicrna.core.tests.html#seismicrna.core.tests.header_test.TestConstants.test_average_prefix">test_average_prefix() (seismicrna.core.tests.header_test.TestConstants method)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_backup_dir">test_backup_dir() (seismicrna.core.io.tests.file_test.TestMakeTempBackup method)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_backup_file">test_backup_file() (seismicrna.core.io.tests.file_test.TestMakeTempBackup method)</a>
 </li>
       <li><a href="api/seismicrna.core.seq.tests.html#seismicrna.core.seq.tests.section_test.TestConstants.test_base_name">test_base_name() (seismicrna.core.seq.tests.section_test.TestConstants method)</a>
 </li>
@@ -8934,10 +9006,10 @@ <h2 id="T">T</h2>
 </li>
       <li><a href="api/seismicrna.core.seq.tests.html#seismicrna.core.seq.tests.xna_test.TestXNA.test_not_equal_rna_str">test_not_equal_rna_str() (seismicrna.core.seq.tests.xna_test.TestXNA method)</a>
 </li>
-  </ul></td>
-  <td style="width: 33%; vertical-align: top;"><ul>
       <li><a href="api/seismicrna.core.rel.tests.html#seismicrna.core.rel.tests.code_test.TestConstants.test_np_type">test_np_type() (seismicrna.core.rel.tests.code_test.TestConstants method)</a>
 </li>
+  </ul></td>
+  <td style="width: 33%; vertical-align: top;"><ul>
       <li><a href="api/seismicrna.core.tests.html#seismicrna.core.tests.header_test.TestClustHeader.test_num_levels">test_num_levels() (seismicrna.core.tests.header_test.TestClustHeader method)</a>
 
       <ul>
@@ -9097,6 +9169,10 @@ <h2 id="T">T</h2>
       <li><a href="api/seismicrna.core.tests.html#seismicrna.core.tests.header_test.TestRelHeader.test_rels_empty">test_rels_empty() (seismicrna.core.tests.header_test.TestRelHeader method)</a>
 </li>
       <li><a href="api/seismicrna.core.tests.html#seismicrna.core.tests.header_test.TestRelHeader.test_rels_normal">test_rels_normal() (seismicrna.core.tests.header_test.TestRelHeader method)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_restore_dir">test_restore_dir() (seismicrna.core.io.tests.file_test.TestMakeTempBackup method)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.TestMakeTempBackup.test_restore_file">test_restore_file() (seismicrna.core.io.tests.file_test.TestMakeTempBackup method)</a>
 </li>
       <li><a href="api/seismicrna.core.seq.tests.html#seismicrna.core.seq.tests.xna_test.TestDNA.test_reverse_complement">test_reverse_complement() (seismicrna.core.seq.tests.xna_test.TestDNA method)</a>
 
@@ -9591,6 +9667,8 @@ <h2 id="T">T</h2>
       <li><a href="api/seismicrna.core.parallel.tests.html#seismicrna.core.parallel.tests.temp_test.TestLockTempDir.TempErrFilter">TestLockTempDir.TempErrFilter (class in seismicrna.core.parallel.tests.temp_test)</a>
 </li>
       <li><a href="api/seismicrna.core.tests.html#seismicrna.core.tests.header_test.TestMakeHeader">TestMakeHeader (class in seismicrna.core.tests.header_test)</a>
+</li>
+      <li><a href="api/seismicrna.core.io.tests.html#seismicrna.core.io.tests.file_test.TestMakeTempBackup">TestMakeTempBackup (class in seismicrna.core.io.tests.file_test)</a>
 </li>
       <li><a href="api/seismicrna.core.mu.tests.html#seismicrna.core.mu.tests.nan_test.TestNoNaN">TestNoNaN (class in seismicrna.core.mu.tests.nan_test)</a>
 </li>
@@ -9756,14 +9834,14 @@ <h2 id="U">U</h2>
 </li>
       <li><a href="api/seismicrna.core.parallel.tests.html#seismicrna.core.parallel.tests.temp_test.unlock">unlock() (in module seismicrna.core.parallel.tests.temp_test)</a>
 </li>
-  </ul></td>
-  <td style="width: 33%; vertical-align: top;"><ul>
       <li><a href="api/seismicrna.core.seq.html#seismicrna.core.seq.section.Section.unmasked">unmasked (seismicrna.core.seq.section.Section property)</a>
 
       <ul>
         <li><a href="api/seismicrna.table.html#seismicrna.table.base.PosTable.unmasked">(seismicrna.table.base.PosTable property)</a>
 </li>
       </ul></li>
+  </ul></td>
+  <td style="width: 33%; vertical-align: top;"><ul>
       <li><a href="api/seismicrna.core.seq.html#seismicrna.core.seq.section.Section.unmasked_bool">unmasked_bool (seismicrna.core.seq.section.Section property)</a>
 
       <ul>
@@ -9776,6 +9854,12 @@ <h2 id="U">U</h2>
         <li><a href="api/seismicrna.table.html#seismicrna.table.base.PosTable.unmasked_int">(seismicrna.table.base.PosTable property)</a>
 </li>
       </ul></li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.save.update_batches">update_batches() (in module seismicrna.cluster.save)</a>
+</li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.update.update_field">update_field() (in module seismicrna.cluster.update)</a>
+</li>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.csv.update_log_counts">update_log_counts() (in module seismicrna.cluster.csv)</a>
+</li>
   </ul></td>
 </tr></table>
 
@@ -9868,7 +9952,7 @@ <h2 id="W">W</h2>
   <td style="width: 33%; vertical-align: top;"><ul>
       <li><a href="api/seismicrna.relate.html#seismicrna.relate.write.write_all">write_all() (in module seismicrna.relate.write)</a>
 </li>
-      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.write.write_batches">write_batches() (in module seismicrna.cluster.write)</a>
+      <li><a href="api/seismicrna.cluster.html#seismicrna.cluster.save.write_batches">write_batches() (in module seismicrna.cluster.save)</a>
 </li>
       <li><a href="api/seismicrna.graph.html#seismicrna.graph.base.GraphBase.write_csv">write_csv() (seismicrna.graph.base.GraphBase method)</a>
 </li>
diff --git a/docs/howto/addclust.html b/docs/howto/addclust.html
new file mode 100644
index 00000000..bebd37e0
--- /dev/null
+++ b/docs/howto/addclust.html
@@ -0,0 +1,192 @@
+<!DOCTYPE html>
+<html class="writer-html5" lang="en" >
+<head>
+  <meta charset="utf-8" /><meta name="generator" content="Docutils 0.17.1: http://docutils.sourceforge.net/" />
+
+  <meta name="viewport" content="width=device-width, initial-scale=1.0" />
+  <title>Add Clusters to an Already-Clustered Dataset &mdash; seismic-rna  documentation</title>
+      <link rel="stylesheet" href="../_static/pygments.css" type="text/css" />
+      <link rel="stylesheet" href="../_static/css/theme.css" type="text/css" />
+    <link rel="shortcut icon" href="../_static/favicon-32x32.ico"/>
+  <!--[if lt IE 9]>
+    <script src="../_static/js/html5shiv.min.js"></script>
+  <![endif]-->
+  
+        <script data-url_root="../" id="documentation_options" src="../_static/documentation_options.js"></script>
+        <script src="../_static/jquery.js"></script>
+        <script src="../_static/underscore.js"></script>
+        <script src="../_static/doctools.js"></script>
+    <script src="../_static/js/theme.js"></script>
+    <link rel="index" title="Index" href="../genindex.html" />
+    <link rel="search" title="Search" href="../search.html" />
+    <link rel="next" title="Clean FASTA Files" href="cleanfa.html" />
+    <link rel="prev" title="Log Messages" href="logging.html" /> 
+</head>
+
+<body class="wy-body-for-nav"> 
+  <div class="wy-grid-for-nav">
+    <nav data-toggle="wy-nav-shift" class="wy-nav-side">
+      <div class="wy-side-scroll">
+        <div class="wy-side-nav-search" >
+
+          
+          
+          <a href="../index.html" class="icon icon-home">
+            seismic-rna
+              <img src="../_static/logo-200.png" class="logo" alt="Logo"/>
+          </a>
+<div role="search">
+  <form id="rtd-search-form" class="wy-form" action="../search.html" method="get">
+    <input type="text" name="q" placeholder="Search docs" aria-label="Search docs" />
+    <input type="hidden" name="check_keywords" value="yes" />
+    <input type="hidden" name="area" value="default" />
+  </form>
+</div>
+        </div><div class="wy-menu wy-menu-vertical" data-spy="affix" role="navigation" aria-label="Navigation menu">
+              <ul class="current">
+<li class="toctree-l1"><a class="reference internal" href="../about/index.html">About</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../whatdoes/index.html">What it Does</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../install/index.html">Install</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../steps/index.html">Step-By-Step</a></li>
+<li class="toctree-l1 current"><a class="reference internal" href="index.html">How To</a><ul class="current">
+<li class="toctree-l2"><a class="reference internal" href="run/index.html">Run SEISMIC-RNA</a></li>
+<li class="toctree-l2"><a class="reference internal" href="graph/index.html">Graph Results</a></li>
+<li class="toctree-l2"><a class="reference internal" href="inputs.html">List Input Files</a></li>
+<li class="toctree-l2"><a class="reference internal" href="sections.html">Define Sections</a></li>
+<li class="toctree-l2"><a class="reference internal" href="normalize.html">Normalize Mutation Rates</a></li>
+<li class="toctree-l2"><a class="reference internal" href="parallel.html">Parallelize Tasks</a></li>
+<li class="toctree-l2"><a class="reference internal" href="logging.html">Log Messages</a></li>
+<li class="toctree-l2 current"><a class="current reference internal" href="#">Add Clusters to an Already-Clustered Dataset</a><ul>
+<li class="toctree-l3"><a class="reference internal" href="#background-about-adding-clusters">Background about adding clusters</a><ul>
+<li class="toctree-l4"><a class="reference internal" href="#purpose-of-adding-clusters">Purpose of adding clusters</a></li>
+</ul>
+</li>
+<li class="toctree-l3"><a class="reference internal" href="#how-to-add-clusters-to-a-cluster-dataset">How to add clusters to a Cluster dataset</a><ul>
+<li class="toctree-l4"><a class="reference internal" href="#command-line-for-adding-clusters">Command line for adding clusters</a></li>
+</ul>
+</li>
+</ul>
+</li>
+<li class="toctree-l2"><a class="reference internal" href="cleanfa.html">Clean FASTA Files</a></li>
+</ul>
+</li>
+<li class="toctree-l1"><a class="reference internal" href="../cli.html">Commands, Arguments, Options</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../api/seismicrna.html">seismicrna package</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../formats/index.html">File Formats</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../data/index.html">Data Structures</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../algos/index.html">Algorithms</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../issues.html">Bugs and Requests</a></li>
+<li class="toctree-l1"><a class="reference internal" href="../writeme.html">How to Write this Manual</a></li>
+</ul>
+
+        </div>
+      </div>
+    </nav>
+
+    <section data-toggle="wy-nav-shift" class="wy-nav-content-wrap"><nav class="wy-nav-top" aria-label="Mobile navigation menu" >
+          <i data-toggle="wy-nav-top" class="fa fa-bars"></i>
+          <a href="../index.html">seismic-rna</a>
+      </nav>
+
+      <div class="wy-nav-content">
+        <div class="rst-content">
+          <div role="navigation" aria-label="Page navigation">
+  <ul class="wy-breadcrumbs">
+      <li><a href="../index.html" class="icon icon-home" aria-label="Home"></a></li>
+          <li class="breadcrumb-item"><a href="index.html">How To</a></li>
+      <li class="breadcrumb-item active">Add Clusters to an Already-Clustered Dataset</li>
+      <li class="wy-breadcrumbs-aside">
+            <a href="../_sources/howto/addclust.rst.txt" rel="nofollow"> View page source</a>
+      </li>
+  </ul>
+  <hr/>
+</div>
+          <div role="main" class="document" itemscope="itemscope" itemtype="http://schema.org/Article">
+           <div itemprop="articleBody">
+             
+  <section id="add-clusters-to-an-already-clustered-dataset">
+<h1>Add Clusters to an Already-Clustered Dataset<a class="headerlink" href="#add-clusters-to-an-already-clustered-dataset" title="Permalink to this headline"></a></h1>
+<section id="background-about-adding-clusters">
+<h2>Background about adding clusters<a class="headerlink" href="#background-about-adding-clusters" title="Permalink to this headline"></a></h2>
+<section id="purpose-of-adding-clusters">
+<h3>Purpose of adding clusters<a class="headerlink" href="#purpose-of-adding-clusters" title="Permalink to this headline"></a></h3>
+<p>After running the Cluster step, you may want to continue clustering using more
+clusters.
+You could accomplish this by simply rerunning the Cluster step using a larger
+<code class="docutils literal notranslate"><span class="pre">--max-clusters</span></code> (<code class="docutils literal notranslate"><span class="pre">-k</span></code>).
+But since Cluster always begins with 1 cluster, you would need to repeat all the
+clusters that you had already run before being able to add more clusters, which
+would waste your computer resources.
+The Add Clusters tool lets you keep your existing clustering results and merely
+append more clusters, which is faster and more efficient.</p>
+</section>
+</section>
+<section id="how-to-add-clusters-to-a-cluster-dataset">
+<h2>How to add clusters to a Cluster dataset<a class="headerlink" href="#how-to-add-clusters-to-a-cluster-dataset" title="Permalink to this headline"></a></h2>
+<section id="command-line-for-adding-clusters">
+<h3>Command line for adding clusters<a class="headerlink" href="#command-line-for-adding-clusters" title="Permalink to this headline"></a></h3>
+<p>Suppose you have already run clustering with a maximum of 2 clusters (<code class="docutils literal notranslate"><span class="pre">-k</span> <span class="pre">2</span></code>)
+on a dataset:</p>
+<div class="highlight-default notranslate"><div class="highlight"><pre><span></span><span class="n">seismic</span> <span class="n">cluster</span> <span class="o">-</span><span class="n">k</span> <span class="mi">2</span> <span class="p">{</span><span class="n">out</span><span class="p">}</span><span class="o">/</span><span class="p">{</span><span class="n">sample</span><span class="p">}</span><span class="o">/</span><span class="n">mask</span><span class="o">/</span><span class="p">{</span><span class="n">ref</span><span class="p">}</span><span class="o">/</span><span class="p">{</span><span class="n">sect</span><span class="p">}</span>
+</pre></div>
+</div>
+<p>To add more clusters, use <code class="docutils literal notranslate"><span class="pre">seismic</span> <span class="pre">+addclust</span></code> with the new maximum number of
+clusters (e.g. 4) and the Cluster report as the input file:</p>
+<div class="highlight-default notranslate"><div class="highlight"><pre><span></span><span class="n">seismic</span> <span class="o">+</span><span class="n">addclust</span> <span class="o">-</span><span class="n">k</span> <span class="mi">4</span> <span class="p">{</span><span class="n">out</span><span class="p">}</span><span class="o">/</span><span class="p">{</span><span class="n">sample</span><span class="p">}</span><span class="o">/</span><span class="n">cluster</span><span class="o">/</span><span class="p">{</span><span class="n">ref</span><span class="p">}</span><span class="o">/</span><span class="p">{</span><span class="n">sect</span><span class="p">}</span>
+</pre></div>
+</div>
+<p>This command will resume clustering with one more than the maximum number of
+existing clusters (in this case, the previous maximum was 2, so <code class="docutils literal notranslate"><span class="pre">+addclust</span></code>
+will begin at 3).
+The maximum number of clusters follows the same rules as in <code class="docutils literal notranslate"><span class="pre">cluster</span></code> (see
+<a class="reference internal" href="run/cluster.html#cluster-max"><span class="std std-ref">Cluster setting: Maximum number of clusters</span></a> for more information).
+You do not need to specify any other settings for clustering, such as the number
+of runs or the threshold for convergence: <code class="docutils literal notranslate"><span class="pre">+addclust</span></code> automatically uses the
+same settings that you used originally in <code class="docutils literal notranslate"><span class="pre">cluster</span></code>.</p>
+<p>You can give any number of Cluster report files as inputs for Add Clusters.
+See <a class="reference internal" href="inputs.html"><span class="doc">List Input Files</span></a> for ways to list multiple files.</p>
+<p>The only new files produced are <code class="docutils literal notranslate"><span class="pre">mus</span></code> and <code class="docutils literal notranslate"><span class="pre">props</span></code> files for each additional
+clustering run.
+The Cluster report file, the batch files, and the counts file are all updated
+in-place, as if you had run <code class="docutils literal notranslate"><span class="pre">cluster</span></code> the first time with the new <code class="docutils literal notranslate"><span class="pre">-k</span></code>.
+Because updating in-place means that there is a risk of data loss if any error
+occurs during writing, the original files are all first backed up in a temporary
+directory, which you can specify with <code class="docutils literal notranslate"><span class="pre">--temp-dir</span></code> (<code class="docutils literal notranslate"><span class="pre">-t</span></code>).
+In case of a fatal error while updating the files, the original files will all
+be restored from the backup, as if you had never run <code class="docutils literal notranslate"><span class="pre">+addclust</span></code>.</p>
+</section>
+</section>
+</section>
+
+
+           </div>
+          </div>
+          <footer><div class="rst-footer-buttons" role="navigation" aria-label="Footer">
+        <a href="logging.html" class="btn btn-neutral float-left" title="Log Messages" accesskey="p" rel="prev"><span class="fa fa-arrow-circle-left" aria-hidden="true"></span> Previous</a>
+        <a href="cleanfa.html" class="btn btn-neutral float-right" title="Clean FASTA Files" accesskey="n" rel="next">Next <span class="fa fa-arrow-circle-right" aria-hidden="true"></span></a>
+    </div>
+
+  <hr/>
+
+  <div role="contentinfo">
+    <p>&#169; Copyright 2024, the Rouskin Lab.</p>
+  </div>
+
+  Built with <a href="https://www.sphinx-doc.org/">Sphinx</a> using a
+    <a href="https://github.com/readthedocs/sphinx_rtd_theme">theme</a>
+    provided by <a href="https://readthedocs.org">Read the Docs</a>.
+   
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+  </script> 
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+</html>
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diff --git a/docs/howto/cleanfa.html b/docs/howto/cleanfa.html
index d10291bb..e1f6a8b8 100644
--- a/docs/howto/cleanfa.html
+++ b/docs/howto/cleanfa.html
@@ -20,7 +20,7 @@
     <link rel="index" title="Index" href="../genindex.html" />
     <link rel="search" title="Search" href="../search.html" />
     <link rel="next" title="Commands, Arguments, Options" href="../cli.html" />
-    <link rel="prev" title="Log Messages" href="logging.html" /> 
+    <link rel="prev" title="Add Clusters to an Already-Clustered Dataset" href="addclust.html" /> 
 </head>
 
 <body class="wy-body-for-nav"> 
@@ -56,6 +56,7 @@
 <li class="toctree-l2"><a class="reference internal" href="normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2 current"><a class="current reference internal" href="#">Clean FASTA Files</a><ul>
 <li class="toctree-l3"><a class="reference internal" href="#background-about-cleaning-fasta-files">Background about cleaning FASTA files</a><ul>
 <li class="toctree-l4"><a class="reference internal" href="#purpose-of-cleaning-fasta-files">Purpose of cleaning FASTA files</a></li>
@@ -194,7 +195,7 @@ <h3>Errors while cleaning FASTA files<a class="headerlink" href="#errors-while-c
            </div>
           </div>
           <footer><div class="rst-footer-buttons" role="navigation" aria-label="Footer">
-        <a href="logging.html" class="btn btn-neutral float-left" title="Log Messages" accesskey="p" rel="prev"><span class="fa fa-arrow-circle-left" aria-hidden="true"></span> Previous</a>
+        <a href="addclust.html" class="btn btn-neutral float-left" title="Add Clusters to an Already-Clustered Dataset" accesskey="p" rel="prev"><span class="fa fa-arrow-circle-left" aria-hidden="true"></span> Previous</a>
         <a href="../cli.html" class="btn btn-neutral float-right" title="Commands, Arguments, Options" accesskey="n" rel="next">Next <span class="fa fa-arrow-circle-right" aria-hidden="true"></span></a>
     </div>
 
diff --git a/docs/howto/graph/index.html b/docs/howto/graph/index.html
index ef4295fd..a3f02772 100644
--- a/docs/howto/graph/index.html
+++ b/docs/howto/graph/index.html
@@ -60,6 +60,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/graph/params.html b/docs/howto/graph/params.html
index cd5fee7c..aa4c65f2 100644
--- a/docs/howto/graph/params.html
+++ b/docs/howto/graph/params.html
@@ -65,6 +65,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/graph/profile.html b/docs/howto/graph/profile.html
index 4862f962..e1ab329e 100644
--- a/docs/howto/graph/profile.html
+++ b/docs/howto/graph/profile.html
@@ -66,6 +66,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/index.html b/docs/howto/index.html
index 6174c93a..f2ee6841 100644
--- a/docs/howto/index.html
+++ b/docs/howto/index.html
@@ -56,6 +56,7 @@
 <li class="toctree-l2"><a class="reference internal" href="normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
@@ -139,6 +140,11 @@ <h1>How To<a class="headerlink" href="#how-to" title="Permalink to this headline
 <li class="toctree-l2"><a class="reference internal" href="logging.html#logging-messages-to-files">Logging messages to files</a></li>
 </ul>
 </li>
+<li class="toctree-l1"><a class="reference internal" href="addclust.html">Add Clusters to an Already-Clustered Dataset</a><ul>
+<li class="toctree-l2"><a class="reference internal" href="addclust.html#background-about-adding-clusters">Background about adding clusters</a></li>
+<li class="toctree-l2"><a class="reference internal" href="addclust.html#how-to-add-clusters-to-a-cluster-dataset">How to add clusters to a Cluster dataset</a></li>
+</ul>
+</li>
 <li class="toctree-l1"><a class="reference internal" href="cleanfa.html">Clean FASTA Files</a><ul>
 <li class="toctree-l2"><a class="reference internal" href="cleanfa.html#background-about-cleaning-fasta-files">Background about cleaning FASTA files</a></li>
 <li class="toctree-l2"><a class="reference internal" href="cleanfa.html#how-to-clean-fasta-files">How to clean FASTA files</a></li>
diff --git a/docs/howto/inputs.html b/docs/howto/inputs.html
index a2242e41..6c9a47b0 100644
--- a/docs/howto/inputs.html
+++ b/docs/howto/inputs.html
@@ -59,6 +59,7 @@
 <li class="toctree-l2"><a class="reference internal" href="normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/logging.html b/docs/howto/logging.html
index 9fe35438..24d8fbfb 100644
--- a/docs/howto/logging.html
+++ b/docs/howto/logging.html
@@ -19,7 +19,7 @@
     <script src="../_static/js/theme.js"></script>
     <link rel="index" title="Index" href="../genindex.html" />
     <link rel="search" title="Search" href="../search.html" />
-    <link rel="next" title="Clean FASTA Files" href="cleanfa.html" />
+    <link rel="next" title="Add Clusters to an Already-Clustered Dataset" href="addclust.html" />
     <link rel="prev" title="Parallelize Tasks" href="parallel.html" /> 
 </head>
 
@@ -70,6 +70,7 @@
 </li>
 </ul>
 </li>
+<li class="toctree-l2"><a class="reference internal" href="addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
@@ -283,7 +284,7 @@ <h3>How to view log files in real time<a class="headerlink" href="#how-to-view-l
           </div>
           <footer><div class="rst-footer-buttons" role="navigation" aria-label="Footer">
         <a href="parallel.html" class="btn btn-neutral float-left" title="Parallelize Tasks" accesskey="p" rel="prev"><span class="fa fa-arrow-circle-left" aria-hidden="true"></span> Previous</a>
-        <a href="cleanfa.html" class="btn btn-neutral float-right" title="Clean FASTA Files" accesskey="n" rel="next">Next <span class="fa fa-arrow-circle-right" aria-hidden="true"></span></a>
+        <a href="addclust.html" class="btn btn-neutral float-right" title="Add Clusters to an Already-Clustered Dataset" accesskey="n" rel="next">Next <span class="fa fa-arrow-circle-right" aria-hidden="true"></span></a>
     </div>
 
   <hr/>
diff --git a/docs/howto/normalize.html b/docs/howto/normalize.html
index b4f87ab0..2f47c83d 100644
--- a/docs/howto/normalize.html
+++ b/docs/howto/normalize.html
@@ -59,6 +59,7 @@
 </li>
 <li class="toctree-l2"><a class="reference internal" href="parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/parallel.html b/docs/howto/parallel.html
index 798d9b0a..d51a81a4 100644
--- a/docs/howto/parallel.html
+++ b/docs/howto/parallel.html
@@ -59,6 +59,7 @@
 </ul>
 </li>
 <li class="toctree-l2"><a class="reference internal" href="logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/run/align.html b/docs/howto/run/align.html
index bb748dad..d8f59b50 100644
--- a/docs/howto/run/align.html
+++ b/docs/howto/run/align.html
@@ -75,6 +75,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/run/cluster.html b/docs/howto/run/cluster.html
index e3729f84..73e52859 100644
--- a/docs/howto/run/cluster.html
+++ b/docs/howto/run/cluster.html
@@ -76,6 +76,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
@@ -138,7 +139,7 @@ <h3>Cluster input file: Mask report<a class="headerlink" href="#cluster-input-fi
 <section id="cluster-settings">
 <h2>Cluster: Settings<a class="headerlink" href="#cluster-settings" title="Permalink to this headline"></a></h2>
 <section id="cluster-setting-maximum-number-of-clusters">
-<h3>Cluster setting: Maximum number of clusters<a class="headerlink" href="#cluster-setting-maximum-number-of-clusters" title="Permalink to this headline"></a></h3>
+<span id="cluster-max"></span><h3>Cluster setting: Maximum number of clusters<a class="headerlink" href="#cluster-setting-maximum-number-of-clusters" title="Permalink to this headline"></a></h3>
 <p>To infer alternative RNA structures, SEISMIC-RNA uses an optimized version of
 our original DREEM algorithm [<a class="reference external" href="https://doi.org/10.1038/s41586-020-2253-5">Tomezsko et al. (2020)</a>], which is based on
 <a class="reference external" href="https://en.wikipedia.org/wiki/Expectation%E2%80%93maximization_algorithm">expectation-maximization</a> (EM).
@@ -167,6 +168,11 @@ <h3>Cluster setting: Maximum number of clusters<a class="headerlink" href="#clus
 the number that yielded the best BIC (field “Optimal Number of Clusters”),
 which is less than or equal to the maximum you specified.</p>
 </div>
+<div class="admonition note">
+<p class="admonition-title">Note</p>
+<p>If you decide after clustering that you wanted to run clustering with more
+clusters, then use the tool <code class="docutils literal notranslate"><span class="pre">+addclust</span></code> (see <a class="reference internal" href="../addclust.html"><span class="doc">Add Clusters to an Already-Clustered Dataset</span></a>).</p>
+</div>
 </section>
 <section id="cluster-setting-expectation-maximization-iterations">
 <h3>Cluster setting: Expectation-maximization iterations<a class="headerlink" href="#cluster-setting-expectation-maximization-iterations" title="Permalink to this headline"></a></h3>
@@ -278,6 +284,10 @@ <h3>Cluster output file: Cluster report<a class="headerlink" href="#cluster-outp
 </section>
 <section id="cluster-troubleshoot-and-optimize">
 <h2>Cluster: Troubleshoot and optimize<a class="headerlink" href="#cluster-troubleshoot-and-optimize" title="Permalink to this headline"></a></h2>
+<section id="run-cluster-with-more-clusters-without-repeating-the-work-already-done">
+<h3>Run Cluster with more clusters, without repeating the work already done<a class="headerlink" href="#run-cluster-with-more-clusters-without-repeating-the-work-already-done" title="Permalink to this headline"></a></h3>
+<p>The tool <code class="docutils literal notranslate"><span class="pre">+addclust</span></code> exists for this purpose: see <a class="reference internal" href="../addclust.html"><span class="doc">Add Clusters to an Already-Clustered Dataset</span></a>.</p>
+</section>
 <section id="cluster-takes-too-long-to-finish">
 <h3>Cluster takes too long to finish<a class="headerlink" href="#cluster-takes-too-long-to-finish" title="Permalink to this headline"></a></h3>
 <ul class="simple">
diff --git a/docs/howto/run/demult.html b/docs/howto/run/demult.html
index cb71954e..6ab6168f 100644
--- a/docs/howto/run/demult.html
+++ b/docs/howto/run/demult.html
@@ -69,6 +69,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/run/export.html b/docs/howto/run/export.html
index 13ff540c..42596757 100644
--- a/docs/howto/run/export.html
+++ b/docs/howto/run/export.html
@@ -74,6 +74,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/run/fold.html b/docs/howto/run/fold.html
index 5ded73fd..f41757bd 100644
--- a/docs/howto/run/fold.html
+++ b/docs/howto/run/fold.html
@@ -75,6 +75,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/run/graph.html b/docs/howto/run/graph.html
index dc095278..afca8200 100644
--- a/docs/howto/run/graph.html
+++ b/docs/howto/run/graph.html
@@ -73,6 +73,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
 </ul>
 </li>
diff --git a/docs/howto/run/index.html b/docs/howto/run/index.html
index 993df7c5..edf28979 100644
--- a/docs/howto/run/index.html
+++ b/docs/howto/run/index.html
@@ -69,6 +69,7 @@
 <li class="toctree-l2"><a class="reference internal" href="../normalize.html">Normalize Mutation Rates</a></li>
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 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
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diff --git a/docs/howto/run/mask.html b/docs/howto/run/mask.html
index 7dd77153..d8063ef0 100644
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 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
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 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
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diff --git a/docs/howto/run/pool.html b/docs/howto/run/pool.html
index 756925c3..2f9998da 100644
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 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
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diff --git a/docs/howto/run/relate.html b/docs/howto/run/relate.html
index 14320388..dc7047e0 100644
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 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
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diff --git a/docs/howto/run/table.html b/docs/howto/run/table.html
index e3736d63..7a54dd50 100644
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diff --git a/docs/howto/run/wf.html b/docs/howto/run/wf.html
index f18780b5..41a0231d 100644
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 <li class="toctree-l2"><a class="reference internal" href="../logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="../addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="../cleanfa.html">Clean FASTA Files</a></li>
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diff --git a/docs/howto/sections.html b/docs/howto/sections.html
index fdbe2509..269b85e3 100644
--- a/docs/howto/sections.html
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 <li class="toctree-l2"><a class="reference internal" href="normalize.html">Normalize Mutation Rates</a></li>
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 <li class="toctree-l2"><a class="reference internal" href="logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="cleanfa.html">Clean FASTA Files</a></li>
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diff --git a/docs/index.html b/docs/index.html
index 6e01b741..996df0b8 100644
--- a/docs/index.html
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@@ -115,6 +115,7 @@ <h1>How to Research RNA Structures with SEISMIC-RNA<a class="headerlink" href="#
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 <li class="toctree-l2"><a class="reference internal" href="howto/parallel.html">Parallelize Tasks</a></li>
 <li class="toctree-l2"><a class="reference internal" href="howto/logging.html">Log Messages</a></li>
+<li class="toctree-l2"><a class="reference internal" href="howto/addclust.html">Add Clusters to an Already-Clustered Dataset</a></li>
 <li class="toctree-l2"><a class="reference internal" href="howto/cleanfa.html">Clean FASTA Files</a></li>
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+       <td></td>
+       <td>&#160;&#160;&#160;
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+     <tr class="cg-1">
+       <td></td>
+       <td>&#160;&#160;&#160;
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for Sequence Compression/Decompression","Algorithms","seismicrna package","seismicrna.align package","seismicrna.align.tests package","seismicrna.cleanfa package","seismicrna.cluster package","seismicrna.core package","seismicrna.core.arg package","seismicrna.core.batch package","seismicrna.core.extern package","seismicrna.core.io package","seismicrna.core.mu package","seismicrna.core.mu.tests package","seismicrna.core.mu.unbias package","seismicrna.core.mu.unbias.tests package","seismicrna.core.ngs package","seismicrna.core.ngs.tests package","seismicrna.core.parallel package","seismicrna.core.parallel.tests package","seismicrna.core.rel package","seismicrna.core.rel.tests package","seismicrna.core.rna package","seismicrna.core.seq package","seismicrna.core.seq.tests package","seismicrna.core.tests package","seismicrna.demult package","seismicrna.export package","seismicrna.fold package","seismicrna.graph package","seismicrna.mask package","seismicrna.pool package","seismicrna.relate package","seismicrna.relate.aux package","seismicrna.relate.aux.tests package","seismicrna.relate.c package","seismicrna.relate.py package","seismicrna.relate.py.tests package","seismicrna.renumct package","seismicrna.sim package","seismicrna.sim.tests package","seismicrna.table package","seismicrna.test package","seismicrna.wf package","Commands, Arguments, Options","Data Structures","Relation Vectors","Relate Data Structures","Read Names Batch","Reference Sequence","Relate Batch","Brickle: Compressed Python Objects","Connectivity Table (CT): RNA secondary structures","Dot-bracket (DB): RNA secondary structures","FASTA: Reference sequences","FASTQ: Sequencing Reads","Data Formats","Sample results: Export data from SEISMIC-RNA to SEISMIC-GRAPH","VARNA Color: Color codes for VARNA","SAM, BAM, and CRAM: Alignment Maps","File Formats","Metadata Formats","Metadata for References","Metadata for Samples","Metadata for Sections","Align Report","Cluster Report","Fold Report","Report 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for Sequence Compression/Decompression","Algorithms","seismicrna package","seismicrna.align package","seismicrna.align.tests package","seismicrna.cleanfa package","seismicrna.cluster package","seismicrna.core package","seismicrna.core.arg package","seismicrna.core.batch package","seismicrna.core.extern package","seismicrna.core.io package","seismicrna.core.io.tests package","seismicrna.core.mu package","seismicrna.core.mu.tests package","seismicrna.core.mu.unbias package","seismicrna.core.mu.unbias.tests package","seismicrna.core.ngs package","seismicrna.core.ngs.tests package","seismicrna.core.parallel package","seismicrna.core.parallel.tests package","seismicrna.core.rel package","seismicrna.core.rel.tests package","seismicrna.core.rna package","seismicrna.core.seq package","seismicrna.core.seq.tests package","seismicrna.core.tests package","seismicrna.demult package","seismicrna.export package","seismicrna.fold package","seismicrna.graph package","seismicrna.mask package","seismicrna.pool package","seismicrna.relate package","seismicrna.relate.aux package","seismicrna.relate.aux.tests package","seismicrna.relate.c package","seismicrna.relate.py package","seismicrna.relate.py.tests package","seismicrna.renumct package","seismicrna.sim package","seismicrna.sim.tests package","seismicrna.table package","seismicrna.test package","seismicrna.wf package","Commands, Arguments, Options","Data Structures","Relation Vectors","Relate Data Structures","Read Names Batch","Reference Sequence","Relate Batch","Brickle: Compressed Python Objects","Connectivity Table (CT): RNA secondary structures","Dot-bracket (DB): RNA secondary structures","FASTA: Reference sequences","FASTQ: Sequencing Reads","Data Formats","Sample results: Export data from SEISMIC-RNA to SEISMIC-GRAPH","VARNA Color: Color codes for VARNA","SAM, BAM, and CRAM: Alignment Maps","File Formats","Metadata Formats","Metadata for References","Metadata for Samples","Metadata for Sections","Align Report","Cluster Report","Fold Report","Report Formats","Mask Report","Pool Report","Relate Report","Add Clusters to an Already-Clustered Dataset","Clean FASTA Files","Graph Results","General parameters for graphing","Profile: Bar graph of relationships(s) per position","How To","List Input Files","Log Messages","Normalize Mutation Rates","Parallelize Tasks","Align: Trim FASTQ files and align them to reference sequences","Cluster: Infer alternative structures by clustering reads\u2019 mutations","Demultiplex: Split multiplexed FASTQ files by their barcodes","Export: Export a file of each sample for the seismic-graph web app","Fold: Predict RNA secondary structures using mutation rates","Graph: Plot data from tables and/or structures and compare samples","Run SEISMIC-RNA","Mask: Define mutations and sections to filter reads and positions","Pool: Combine samples from the Relate step","Relate: Compute relationships between references and aligned reads","Table: Count mutations for each read and position","Workflow: Run all steps","Define Sections","How to Research RNA Structures with SEISMIC-RNA","Installing with Conda","Install","Prerequisites: Python, pip, and Conda","Virtual Environments","Bugs and Requests","Step-by-step walkthrough","Example dataset","Step-By-Step","What it Does","How to Write this Manual"],titleterms:{"1":61,"1st":61,"2":[61,88],"256":108,"2nd":61,"3":56,"5":56,"default":93,"export":[33,63,91],"long":89,"new":[81,106],"while":[79,95,97],A:95,By:109,To:83,about:[3,68,69,70,78,79],activ:[102,105],ad:78,adapt:88,add:78,algorithm:[5,6,79],align:[8,9,50,65,71,88,97],all:[81,99],alphabet:61,alreadi:[78,89],altern:89,ambigu:[52,97],an:78,app:91,ar:[85,95],arbitari:95,arg:13,argument:50,assess:88,aucrol:50,author:0,automat:100,aux:[39,40],auxiliari:39,background:[5,78,79],bam:65,bar:82,barcod:90,base:[52,88,95],batch:[14,54,56,87,89,95,97],benefit:87,between:[52,88,95,97],bodi:[58,59,60],bowti:88,bracket:[59,92],brickl:57,bug:106,build:88,c:41,call:[52,88,110],can:88,cannot:96,caus:96,cgroup:81,channel:88,charact:61,check:[88,104],checksum:108,chemistri:88,choos:88,clean:[10,79],cleanfa:[10,50],cluster:[11,50,63,72,78,81,89,92,98],code:64,coexist:105,color:[64,92],combin:96,come:[85,88],command:[13,50,68,69,70,78,79],compar:93,comparison:[11,65],compress:[5,57],comput:[97,108],conda:[102,104],conflict:105,connect:[58,92],consensu:52,content:[57,58,59,60,61,63,64,65],control:85,coordin:100,core:[12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31],corrol:50,count:[81,98,110],cover:52,cram:65,crash:[88,95,97],creat:[102,105],ct:[44,58],curl:108,custom:88,cutadapt:88,cycl:88,dark:88,data:[51,53,62,63,81,93,96],dataset:[78,108],db:59,deactiv:105,decompress:5,defin:[92,95,100,110],delet:[52,97],delprof:50,demult:[32,50],demultiplex:[88,90],depend:102,destin:81,dir:81,dm:1,dna:61,doe:110,done:89,dot:[59,92],download:108,dreem:2,duplic:96,each:[82,88,91,98,110],effort:105,encod:[52,61,65,88],end:[52,56,61,88],ended:[61,65,88],enhanc:106,entir:50,environ:[102,105],error:79,exampl:[5,54,55,56,68,69,70,71,72,73,75,76,77,82,108],exclud:95,expect:[88,89],extens:[57,58,59,60,61,63,64,65],extern:15,extra:[50,88],fasta:[10,60,79],fastq:[61,88,90],fastqc:88,featur:106,feedback:106,few:[95,97],fewer:88,field:[68,69,70,71,72,73,75,76,77],file:[8,57,58,59,60,61,63,64,65,66,79,81,82,84,85,88,89,90,91,92,95,96,97,98,99,100,107,108],filter:[88,95,110],finish:89,five:85,fold:[34,50,73,92],format:[57,58,59,60,61,62,63,64,65,66,67,74,85,88],fraction:[82,95],from:[63,88,93,96,108],full:100,further:88,g:95,gener:81,github:108,give:106,got:96,graph:[35,50,63,80,81,82,91,93],group:81,guidelin:106,hang:[88,95,97],header:[58,59,60],histpo:50,histread:50,how:[78,79,83,84,85,86,88,100,101,105,106,111],illumina:88,index:88,individu:50,infer:89,inform:[82,95],initi:104,input:[57,58,60,61,63,65,81,82,84,88,89,91,92,95,96,97,98,99],insert:[52,97],instal:[102,103,104],insuffici:97,interfac:13,interleav:[61,88],io:[16,17],irreconcil:52,iter:[39,89],its:102,label:61,layer:63,length:100,let:105,level:85,licens:4,line:[13,58,59,60,78,79],list:84,local:88,log:85,loss:96,low:[52,88],main:[7,38,49],manag:105,mani:95,manual:111,map:[65,88,97],mapseq:1,mask:[36,50,75,89,92,95,110],mate:61,maxim:89,maximum:89,md5:108,membership:89,messag:85,metadata:[63,67,68,69,70,91],minimum:97,modul:[7,8,10,11,38,39,44,49],more:89,mu:[18,19,20,21],multipl:[88,100],multiplex:90,mutat:[56,82,86,89,92,95,98,110],name:[11,54,96,97,100],ng:[22,23],non:102,normal:[81,86,92],note:[68,69,70],number:[56,82,88,89,95],o:81,object:57,one:[88,100],optim:[88,89,95,96,97,99],option:[50,88],order:81,other:106,out:[81,95],output:[57,58,59,60,61,63,64,65,81,82,85,88,89,91,92,95,96,97,98,99],overal:88,overwrit:96,packag:[7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49],pair:[52,61,88],parallel:[24,25,87],paramet:[81,92],pars:[57,58,59,60,61,63,64,65],path:[57,58,59,60,61,63,64,65,85],per:[63,82,98],phred:[61,97],pip:104,pipelin:49,plain:[68,69,70],plot:93,poli:95,pool:[37,76,96],posit:[52,56,63,82,92,95,98,110],pre:88,predict:92,prerequisit:104,pretti:[68,69,70],primari:52,primer:100,problem:106,procedur:5,produc:[88,95,97],profil:[50,63,82],purpos:[12,68,69,70,78,79],py:[42,43],python:[57,102,104],q:81,qualiti:[52,61,65,88,97],quantil:[81,86,92],r:81,rate:[82,86,92],ratio:81,read:[39,52,54,56,61,63,82,88,89,95,97,98,110],real:85,refer:[52,55,60,63,68,88,97,100],rel:[26,27,81],relat:[38,39,40,41,42,43,50,52,53,56,77,95,96,97],relationship:[52,81,82,97,98,110],renumb:44,renumct:[44,50],repeat:89,report:[71,72,73,74,75,76,77,88,89,92,95,96,97,98,106],request:106,research:101,result:[63,80,91],rna:[7,28,58,59,63,92,94,101,102],rnastructur:92,roc:50,run:[50,89,94,99],s:[81,82],sam:[8,65],sampl:[63,69,88,91,93,96],scatter:50,score:[61,65,88,97],secondari:[58,59,92],section:[63,70,92,95,100,110],seismic:[7,50,63,91,94,101,102],seismicrna:[7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49],separ:[61,88],seq:[29,30],sequenc:[5,55,60,61,88,95,97,100],set:[61,82,88,89,91,92,95,96,97,98,99,105,107],sha:108,share:97,sim:[45,46],simul:8,simultan:100,singl:[61,88],size:97,slowli:88,softwar:105,sofwar:105,sourc:88,space:95,spe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\ No newline at end of file
diff --git a/src/docs/api/seismicrna.cluster.rst b/src/docs/api/seismicrna.cluster.rst
index 09efaccd..15e06826 100644
--- a/src/docs/api/seismicrna.cluster.rst
+++ b/src/docs/api/seismicrna.cluster.rst
@@ -64,12 +64,24 @@ Submodules
    :show-inheritance:
 
 
+.. automodule:: seismicrna.cluster.save
+   :members:
+   :undoc-members:
+   :show-inheritance:
+
+
 .. automodule:: seismicrna.cluster.uniq
    :members:
    :undoc-members:
    :show-inheritance:
 
 
+.. automodule:: seismicrna.cluster.update
+   :members:
+   :undoc-members:
+   :show-inheritance:
+
+
 .. automodule:: seismicrna.cluster.write
    :members:
    :undoc-members:
diff --git a/src/docs/api/seismicrna.core.io.rst b/src/docs/api/seismicrna.core.io.rst
index bffcb1df..771dea99 100644
--- a/src/docs/api/seismicrna.core.io.rst
+++ b/src/docs/api/seismicrna.core.io.rst
@@ -6,6 +6,14 @@ seismicrna.core.io package
    :undoc-members:
    :show-inheritance:
 
+Subpackages
+-----------
+
+.. toctree::
+   :maxdepth: 4
+
+   seismicrna.core.io.tests
+
 Submodules
 ----------
 
diff --git a/src/docs/api/seismicrna.core.io.tests.rst b/src/docs/api/seismicrna.core.io.tests.rst
new file mode 100644
index 00000000..a2c6687a
--- /dev/null
+++ b/src/docs/api/seismicrna.core.io.tests.rst
@@ -0,0 +1,16 @@
+seismicrna.core.io.tests package
+================================
+
+.. automodule:: seismicrna.core.io.tests
+   :members:
+   :undoc-members:
+   :show-inheritance:
+
+Submodules
+----------
+
+
+.. automodule:: seismicrna.core.io.tests.file_test
+   :members:
+   :undoc-members:
+   :show-inheritance:
diff --git a/src/docs/howto/addclust.rst b/src/docs/howto/addclust.rst
new file mode 100644
index 00000000..d0f84952
--- /dev/null
+++ b/src/docs/howto/addclust.rst
@@ -0,0 +1,57 @@
+
+Add Clusters to an Already-Clustered Dataset
+--------------------------------------------------------------------------------
+
+Background about adding clusters
+^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
+
+Purpose of adding clusters
+""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
+
+After running the Cluster step, you may want to continue clustering using more
+clusters.
+You could accomplish this by simply rerunning the Cluster step using a larger
+``--max-clusters`` (``-k``).
+But since Cluster always begins with 1 cluster, you would need to repeat all the
+clusters that you had already run before being able to add more clusters, which
+would waste your computer resources.
+The Add Clusters tool lets you keep your existing clustering results and merely
+append more clusters, which is faster and more efficient.
+
+How to add clusters to a Cluster dataset
+^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
+
+Command line for adding clusters
+""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
+
+Suppose you have already run clustering with a maximum of 2 clusters (``-k 2``)
+on a dataset::
+
+    seismic cluster -k 2 {out}/{sample}/mask/{ref}/{sect}
+
+To add more clusters, use ``seismic +addclust`` with the new maximum number of
+clusters (e.g. 4) and the Cluster report as the input file::
+
+    seismic +addclust -k 4 {out}/{sample}/cluster/{ref}/{sect}
+
+This command will resume clustering with one more than the maximum number of
+existing clusters (in this case, the previous maximum was 2, so ``+addclust``
+will begin at 3).
+The maximum number of clusters follows the same rules as in ``cluster`` (see
+:ref:`cluster_max` for more information).
+You do not need to specify any other settings for clustering, such as the number
+of runs or the threshold for convergence: ``+addclust`` automatically uses the
+same settings that you used originally in ``cluster``.
+
+You can give any number of Cluster report files as inputs for Add Clusters.
+See :doc:`./inputs` for ways to list multiple files.
+
+The only new files produced are ``mus`` and ``props`` files for each additional
+clustering run.
+The Cluster report file, the batch files, and the counts file are all updated
+in-place, as if you had run ``cluster`` the first time with the new ``-k``.
+Because updating in-place means that there is a risk of data loss if any error
+occurs during writing, the original files are all first backed up in a temporary
+directory, which you can specify with ``--temp-dir`` (``-t``).
+In case of a fatal error while updating the files, the original files will all
+be restored from the backup, as if you had never run ``+addclust``.
diff --git a/src/docs/howto/index.rst b/src/docs/howto/index.rst
index 21648e5e..dd67a37c 100644
--- a/src/docs/howto/index.rst
+++ b/src/docs/howto/index.rst
@@ -12,4 +12,5 @@ How To
     normalize
     parallel
     logging
+    addclust
     cleanfa
diff --git a/src/docs/howto/run/cluster.rst b/src/docs/howto/run/cluster.rst
index 36c1bed1..0c495b19 100644
--- a/src/docs/howto/run/cluster.rst
+++ b/src/docs/howto/run/cluster.rst
@@ -26,6 +26,8 @@ To cluster all masked relation vectors in ``{out}``, you can use the command ::
 Cluster: Settings
 ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
 
+.. _cluster_max:
+
 Cluster setting: Maximum number of clusters
 """"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
 
@@ -57,6 +59,10 @@ This process continues until one of two limits is reached:
     the number that yielded the best BIC (field "Optimal Number of Clusters"),
     which is less than or equal to the maximum you specified.
 
+.. note::
+    If you decide after clustering that you wanted to run clustering with more
+    clusters, then use the tool ``+addclust`` (see :doc:`../addclust`).
+
 Cluster setting: Expectation-maximization iterations
 """"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
 
@@ -175,6 +181,11 @@ In your cluster report:
 Cluster: Troubleshoot and optimize
 ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
 
+Run Cluster with more clusters, without repeating the work already done
+""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
+
+The tool ``+addclust`` exists for this purpose: see :doc:`../addclust`.
+
 Cluster takes too long to finish
 """"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""