diff --git a/README.md b/README.md
index 7f7c8daf..8d833ab9 100644
--- a/README.md
+++ b/README.md
@@ -136,7 +136,7 @@ use the the `aviary configure` module to update the environment variables used b
 
 These environment variables can also be configured manually, just set the following variables in your `.bashrc` file:
 ```
-export GTDBTK_DATA_PATH=/path/to/gtdb/gtdb_release207/db/ # https://gtdb.ecogenomic.org/downloads
+export GTDBTK_DATA_PATH=/path/to/gtdb/gtdb_release220/db/ # https://gtdb.ecogenomic.org/downloads
 export EGGNOG_DATA_DIR=/path/to/eggnog-mapper/2.1.8/ # https://github.com/eggnogdb/eggnog-mapper/wiki/eggNOG-mapper-v2.1.5-to-v2.1.8#setup
 export SINGLEM_METAPACKAGE_PATH=/path/to/singlem_metapackage.smpkg/
 export CHECKM2DB=/path/to/checkm2db/