diff --git a/.changeset/nasty-pandas-accept.md b/.changeset/nasty-pandas-accept.md
new file mode 100644
index 00000000000..ddae17ecf52
--- /dev/null
+++ b/.changeset/nasty-pandas-accept.md
@@ -0,0 +1,5 @@
+---
+"@remix-run/dev": patch
+---
+
+Remove `@remix-run/node` from Vite plugin's `optimizeDeps.include` list since it was unnecessary and resulted in Vite warnings when not depending on this package.
diff --git a/packages/remix-dev/vite/plugin.ts b/packages/remix-dev/vite/plugin.ts
index b5c0e7b2ad3..927322d97c0 100644
--- a/packages/remix-dev/vite/plugin.ts
+++ b/packages/remix-dev/vite/plugin.ts
@@ -1089,13 +1089,6 @@ export const remixVitePlugin: RemixVitePlugin = (remixUserConfig = {}) => {
               // That means that before Vite pre-bundles dependencies (e.g. first time dev server is run)
               // mismatching Remix routers cause `Error: You must render this element inside a <Remix> element`.
               "@remix-run/react",
-
-              // For some reason, the `vite-dotenv` integration test consistently fails on webkit
-              // with `504 (Outdated Optimize Dep)` from Vite  unless `@remix-run/node` is included
-              // in `optimizeDeps.include`. 🤷
-              // This could be caused by how we copy `node_modules/` into integration test fixtures,
-              // so maybe this will be unnecessary once we switch to pnpm
-              "@remix-run/node",
             ],
           },
           esbuild: {