diff --git a/pkg/model/cluster.go b/pkg/model/cluster.go
index 8d0e8ca2..7bd3c736 100644
--- a/pkg/model/cluster.go
+++ b/pkg/model/cluster.go
@@ -733,12 +733,15 @@ type ServiceClusters interface {
 	GetExtendedCacheValue(pluginIndex int) interface{}
 	// SetExtendedCacheValue 设置扩展的缓存值，需要预初始化好，否则会有并发修改的问题
 	SetExtendedCacheValue(pluginIndex int, value interface{})
+	// GetInstancesWithMetaValuesNotEqual 获取该标签不等于指定标签值的实例集合
+	GetInstancesWithMetaValuesNotEqual(location Location, metaKey string, metavalue string) map[string]string
 }
 
 // LocationBasedMetaKey 基于某个地域信息的元数据查询key
 type LocationBasedMetaKey struct {
 	location Location
 	metaKey  string
+	keyType  int // 0: equal, 1: not equal
 }
 
 // metaDataInService 标签缓存信息
@@ -1014,6 +1017,42 @@ func (c *clusterCache) SetExtendedCacheValue(pluginIndex int, value interface{})
 	c.extendedCacheValues.Store(pluginIndex, value)
 }
 
+// GetInstancesWithMetaValuesNotEqual 获取该标签不等于指定标签值的实例集合
+func (c *clusterCache) GetInstancesWithMetaValuesNotEqual(location Location, metaKey string, metaValue string) map[string]string {
+	var value interface{}
+	var exists bool
+	locationKey := LocationBasedMetaKey{location: location, metaKey: metaKey, keyType: 1}
+	value, exists = c.svcLevelMetadata.metaDataSet.Load(locationKey)
+	if exists {
+		return value.(map[string]string)
+	}
+	// 按需构建指定地域的实例元数据集合
+	instances := c.svcInstances.GetInstances()
+	metaValueSet := make(map[string]string, 0)
+	for _, instance := range instances {
+		if !matchLocation(instance, location) {
+			continue
+		}
+		if instance.IsIsolated() || instance.GetWeight() == 0 || !instance.IsHealthy() {
+			continue
+		}
+		metadata := instance.GetMetadata()
+		if len(metadata) == 0 {
+			continue
+		}
+		val, ok := metadata[metaKey]
+		if !ok {
+			continue
+		}
+		if val == metaValue {
+			continue
+		}
+		metaValueSet[val] = buildComposedValue(metaKey, val)
+	}
+	value, _ = c.svcLevelMetadata.metaDataSet.LoadOrStore(locationKey, metaValueSet)
+	return value.(map[string]string)
+}
+
 // matchMetadata 标签匹配
 func (c *Cluster) matchMetadata(instance Instance) bool {
 	if c.MetaCount == 0 {
diff --git a/plugin/servicerouter/rulebase/base.go b/plugin/servicerouter/rulebase/base.go
index ea25f2ee..05f6d320 100644
--- a/plugin/servicerouter/rulebase/base.go
+++ b/plugin/servicerouter/rulebase/base.go
@@ -359,14 +359,19 @@ func (g *RuleBasedInstancesFilter) matchDstMetadata(routeInfo *servicerouter.Rou
 			// 首先如果元数据的value无法获取，直接匹配失败
 			return nil, false, "", nil
 		}
+
 		// 全匹配类型直接返回全量实例
 		if ruleMetaValueStr == matchAll && ruleMetaValue.ValueType == apimodel.MatchString_TEXT {
 			return cls, true, "", nil
 		}
-		metaValues := svcCache.GetInstanceMetaValues(cls.Location, ruleMetaKey)
-		if len(metaValues) == 0 {
-			// 不匹配
-			return nil, false, "", nil
+		// 如果是“不等于”类型，需要单独处理
+		var metaValues map[string]string
+		if ruleMetaValue.Type != apimodel.MatchString_NOT_EQUALS {
+			metaValues = svcCache.GetInstanceMetaValues(cls.Location, ruleMetaKey)
+			if len(metaValues) == 0 {
+				// 不匹配
+				return nil, false, "", nil
+			}
 		}
 		switch ruleMetaValue.Type {
 		case apimodel.MatchString_REGEX:
@@ -399,6 +404,17 @@ func (g *RuleBasedInstancesFilter) matchDstMetadata(routeInfo *servicerouter.Rou
 			if !hasMatchedValue {
 				return nil, false, "", nil
 			}
+
+		case apimodel.MatchString_NOT_EQUALS:
+			metaValues = svcCache.GetInstancesWithMetaValuesNotEqual(cls.Location, ruleMetaKey, ruleMetaValueStr)
+			if len(metaValues) == 0 {
+				return cls, false, "", nil
+			}
+			for k, v := range metaValues {
+				cls.RuleAddMetadata(ruleMetaKey, k, v)
+			}
+			metaChanged = true
+
 		// parameter、variable、text 的 exact 最终都是要精确匹配，只是匹配的值来源不同
 		default:
 			// 校验从上一个路由插件继承下来的规则是否符合该目标规则