From d0752053367e45eef4ed7c4ef5e7d342e68e1cde Mon Sep 17 00:00:00 2001 From: Jayoung Ryu Date: Thu, 9 Nov 2023 12:08:44 -0500 Subject: [PATCH] add evidence metrics to output --- bean/preprocessing/utils.py | 11 +++ bin/bean-run | 2 + tests/data/tiling_mini_screen_annotated.h5ad | Bin 0 -> 2131872 bytes tests/data/var_mini_screen_annotated.h5ad | Bin 0 -> 2921120 bytes tests/test_run.py | 80 +++++++++++++++++++ 5 files changed, 93 insertions(+) create mode 100644 tests/data/tiling_mini_screen_annotated.h5ad create mode 100644 tests/data/var_mini_screen_annotated.h5ad create mode 100644 tests/test_run.py diff --git a/bean/preprocessing/utils.py b/bean/preprocessing/utils.py index 1972fa5..6a95b8d 100644 --- a/bean/preprocessing/utils.py +++ b/bean/preprocessing/utils.py @@ -228,3 +228,14 @@ def _obtain_effective_edit_rate(ndata, count_thres=10): def _obtain_n_guides_alleles_per_variant(ndata): return (ndata.allele_to_edit.sum(axis=1) > 0).sum(axis=0) + + +def _obtain_n_cooccurring_variants(ndata) -> np.ndarray: + """Obtain the number of co-occurring variants in any allele produced by any guides.""" + allele_to_edit = ndata.allele_to_edit.cpu() + n_coedited_vars_edits = [] + for edit_idx in range(allele_to_edit.shape[-1]): + gidx, aidx = torch.where(allele_to_edit[:, :, edit_idx] > 0) + n_coedited_vars = (allele_to_edit[gidx, aidx, :].sum(axis=0) > 0).sum() - 1 + n_coedited_vars_edits.append(n_coedited_vars) + return np.array(n_coedited_vars_edits) diff --git a/bin/bean-run b/bin/bean-run index 1b358ee..d3bd879 100644 --- a/bin/bean-run +++ b/bin/bean-run @@ -24,6 +24,7 @@ from bean.preprocessing.data_class import ( from bean.preprocessing.utils import ( _obtain_effective_edit_rate, _obtain_n_guides_alleles_per_variant, + _obtain_n_cooccurring_variants, ) import bean as be @@ -196,6 +197,7 @@ def main(args, bdata): ) target_info_df["effective_edit_rate"] = _obtain_effective_edit_rate(ndata).cpu() target_info_df["n_guides"] = _obtain_n_guides_alleles_per_variant(ndata).cpu() + target_info_df["n_coocc"] = _obtain_n_cooccurring_variants(ndata) if args.adjust_confidence_by_negative_control: adj_negctrl_idx = np.where(target_info_df.ref == target_info_df.alt)[0] print("syn idx: ", adj_negctrl_idx) diff --git a/tests/data/tiling_mini_screen_annotated.h5ad b/tests/data/tiling_mini_screen_annotated.h5ad new file mode 100644 index 0000000000000000000000000000000000000000..9eae70fb100ae7fe0694e0815de3b88d2cc67d08 GIT binary patch literal 2131872 zcmeF431ALa_x~r>*kj-8v8T2s)~IEk5oBTwf>0z8At928grJC}mfF&mqODrG>55j- z){?gNt=1MT4YkBts#JCRpELJ+pJy`36DjikfA7m>p3j_f&z!mU&Ru5iESagdZC|8d zg@OtPcXuU^;$f`HpMMaRO}7~;Da-f}thj+Qz?HegiW_<4xN^{Yz`rfGP^3T~*u7ml zMaiW||B{GSuICYJHYj52iaq*BgU9@zE&;ur7Qh)?ja(W6t?w)(>Vcb*Kl6I#CE{ZR z$R=~UwBVwKXK9DsD8*4Qbh;;w{8hs6wvj2==yeoSmQ#gun*Gq&W` zBzEl%djpgT3PNSX7gj`$+-Q&K{EZ5a8yOiBEw;M1-@GQj+yvcdC&vCA`S&zjx#bdy zC*W9g0E%Ljb5qM@%i^{w)RH;al(Ozh*@?!wqU44DT)BipceM1}ZnDX*M#~i&JZQO@ 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