diff --git a/sdk/src/org.graalvm.polyglot/src/org/graalvm/polyglot/impl/AbstractPolyglotImpl.java b/sdk/src/org.graalvm.polyglot/src/org/graalvm/polyglot/impl/AbstractPolyglotImpl.java
index 597d5f4dca18..79038185b3b5 100644
--- a/sdk/src/org.graalvm.polyglot/src/org/graalvm/polyglot/impl/AbstractPolyglotImpl.java
+++ b/sdk/src/org.graalvm.polyglot/src/org/graalvm/polyglot/impl/AbstractPolyglotImpl.java
@@ -487,6 +487,10 @@ public final AbstractPolyglotImpl getNext() {
         return next;
     }
 
+    public final AbstractPolyglotImpl getNextOrNull() {
+        return next;
+    }
+
     public final void setIO(IOAccessor ioAccess) {
         Objects.requireNonNull(ioAccess, "IOAccess must be non null.");
         this.io = ioAccess;
diff --git a/truffle/src/com.oracle.truffle.api/src/com/oracle/truffle/api/impl/Accessor.java b/truffle/src/com.oracle.truffle.api/src/com/oracle/truffle/api/impl/Accessor.java
index 181c9add1d9e..c6430f57e788 100644
--- a/truffle/src/com.oracle.truffle.api/src/com/oracle/truffle/api/impl/Accessor.java
+++ b/truffle/src/com.oracle.truffle.api/src/com/oracle/truffle/api/impl/Accessor.java
@@ -755,6 +755,8 @@ public abstract <T, G> Iterator<T> mergeHostGuestFrames(Object polyglotEngine, S
         public abstract TruffleFile getInternalResource(Object owner, String resourceId) throws IOException;
 
         public abstract Collection<String> getResourceIds(String componentId);
+
+        public abstract void setIsolatePolyglot(AbstractPolyglotImpl instance);
     }
 
     public abstract static class LanguageSupport extends Support {
diff --git a/truffle/src/com.oracle.truffle.polyglot/src/com/oracle/truffle/polyglot/EngineAccessor.java b/truffle/src/com.oracle.truffle.polyglot/src/com/oracle/truffle/polyglot/EngineAccessor.java
index 82bd35560852..ecb4d4f4ca63 100644
--- a/truffle/src/com.oracle.truffle.polyglot/src/com/oracle/truffle/polyglot/EngineAccessor.java
+++ b/truffle/src/com.oracle.truffle.polyglot/src/com/oracle/truffle/polyglot/EngineAccessor.java
@@ -1988,7 +1988,12 @@ public Object installGuestToHostCodeCache(Object polyglotContextImpl, Object cac
 
         @Override
         public AutoCloseable createPolyglotThreadScope() {
-            return PolyglotImpl.findInstance().getRootImpl().createThreadScope();
+            AbstractPolyglotImpl impl = PolyglotImpl.findIsolatePolyglot();
+            if (impl != null) {
+                return impl.createThreadScope();
+            } else {
+                return null;
+            }
         }
 
         @Override
@@ -2117,6 +2122,11 @@ public Collection<String> getResourceIds(String componentId) {
             }
             throw new IllegalArgumentException(componentId);
         }
+
+        @Override
+        public void setIsolatePolyglot(AbstractPolyglotImpl instance) {
+            PolyglotImpl.setIsolatePolyglot(instance);
+        }
     }
 
     abstract static class AbstractClassLoaderSupplier implements Supplier<ClassLoader> {
diff --git a/truffle/src/com.oracle.truffle.polyglot/src/com/oracle/truffle/polyglot/PolyglotImpl.java b/truffle/src/com.oracle.truffle.polyglot/src/com/oracle/truffle/polyglot/PolyglotImpl.java
index 35ec6b4e73c6..16c61b7e35bf 100644
--- a/truffle/src/com.oracle.truffle.polyglot/src/com/oracle/truffle/polyglot/PolyglotImpl.java
+++ b/truffle/src/com.oracle.truffle.polyglot/src/com/oracle/truffle/polyglot/PolyglotImpl.java
@@ -122,6 +122,8 @@ public final class PolyglotImpl extends AbstractPolyglotImpl {
     private PolyglotValueDispatch disconnectedBigIntegerHostValue;
     private volatile Object defaultFileSystemContext;
 
+    private static volatile AbstractPolyglotImpl isolatePolyglot;
+
     /**
      * Internal method do not use.
      */
@@ -158,6 +160,16 @@ static PolyglotImpl findInstance() {
         return (PolyglotImpl) polyglot;
     }
 
+    static AbstractPolyglotImpl findIsolatePolyglot() {
+        return isolatePolyglot;
+    }
+
+    static void setIsolatePolyglot(AbstractPolyglotImpl instance) {
+        assert instance != null;
+        assert isolatePolyglot == null;
+        isolatePolyglot = instance;
+    }
+
     PolyglotEngineImpl getPreinitializedEngine() {
         return preInitializedEngineRef.get();
     }
@@ -174,13 +186,21 @@ public void initialize() {
     @Override
     public Object initializeModuleToUnnamedAccess(Lookup unnamedLookup, Object unnamedAccess, Object unnamedAPIAccess, Object unnamedIOAccess, Object unnamedManagementAccess) {
         ModuleToUnnamedBridge bridge = ModuleToUnnamedBridge.create(unnamedLookup, unnamedAccess, unnamedAPIAccess, unnamedIOAccess, unnamedManagementAccess);
-        setConstructors(Objects.requireNonNull(bridge.getAPIAccess()));
-        setIO(Objects.requireNonNull(bridge.getIOAccess()));
-        setMonitoring(Objects.requireNonNull(bridge.getManagementAccess()));
-        initialize();
+        AbstractPolyglotImpl impl = getRootImpl();
+        while (impl != null) {
+            initializeModuleToUnnamedBridge(impl, bridge);
+            impl = impl.getNextOrNull();
+        }
         return bridge.getModuleAccess();
     }
 
+    private static void initializeModuleToUnnamedBridge(AbstractPolyglotImpl impl, ModuleToUnnamedBridge bridge) {
+        impl.setConstructors(Objects.requireNonNull(bridge.getAPIAccess()));
+        impl.setIO(Objects.requireNonNull(bridge.getIOAccess()));
+        impl.setMonitoring(Objects.requireNonNull(bridge.getManagementAccess()));
+        impl.initialize();
+    }
+
     @Override
     public Object buildLimits(long statementLimit, Predicate<Object> statementLimitSourceFilter,
                     Consumer<Object> onLimit) {