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In-Incubator Microscope with fluorescence module

This is the repository for the in-incubator microscope. It is capable to acquire Time-Lapse videos from living cells inside an incubator.

The current version of the Z-stage is inspired by the flexure-bearing Z-stage from Richard Bowman's openflexure microscope design. Their open-source project can be found here.

Each functional block has an Arduino acting as a BUS-communicator. Therefore it's possible to control (i.e. switch on/off) devices from the MASTER-device (i.e. Raspberry pi). We aim to build a low-cost solution for high throughput acquisitions, acting as a new tools for (not only) biologists.

It is a very early developer version. Please feel free to contribute to the documentation and device development.

Fluorescence version:

Devices features:

  • X/Y/Z/t Acquisitions possible
  • Fluorescent imaging
  • Quantitative Phase Imaging
  • modularized design
  • Low-cost acquisition
  • based on off-the-shelf components
  • Open-Source

Parts

The Bill of Materials is always the most up-to-date version!

Modules for this setup

Name Properties Price Link #
Baseplate puzzle - 5€ Base-plate 5
Module: Z-Stage - ?? € Z-Stage 1
Module: Mirror 45° - 5€ Mirror 45 1
Module: Raspberry Camera - ??€ RaspiCam 1
Module: LED - ??€ LED 1
Module: Dichroic Beam splitter - ??€ Dichroic Beam splitter 1

3D-printing

To acquire the STL-files use the UC2-Configurator. The files themselves are in the RAW folder. The module can be built using injection-moulded (IM) or 3D-printed (3DP) cubes.

Additional components

  • Check out the RESOURCES for more information!

Results

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