diff --git a/propagator/opentelemetry-propagator-b3/pyproject.toml b/propagator/opentelemetry-propagator-b3/pyproject.toml
index 9e17f4c8d3e..736006e74e4 100644
--- a/propagator/opentelemetry-propagator-b3/pyproject.toml
+++ b/propagator/opentelemetry-propagator-b3/pyproject.toml
@@ -29,9 +29,6 @@ dependencies = [
   "opentelemetry-api ~= 1.3",
 ]
 
-[project.optional-dependencies]
-test = []
-
 [project.entry-points.opentelemetry_propagator]
 b3 = "opentelemetry.propagators.b3:B3SingleFormat"
 b3multi = "opentelemetry.propagators.b3:B3MultiFormat"
diff --git a/propagator/opentelemetry-propagator-b3/test-requirements.txt b/propagator/opentelemetry-propagator-b3/test-requirements.txt
new file mode 100644
index 00000000000..366dfca0d89
--- /dev/null
+++ b/propagator/opentelemetry-propagator-b3/test-requirements.txt
@@ -0,0 +1,18 @@
+asgiref==3.7.2
+attrs==23.2.0
+Deprecated==1.2.14
+flaky==3.7.0
+importlib-metadata==6.11.0
+iniconfig==2.0.0
+packaging==23.2
+pluggy==1.4.0
+py==1.11.0
+py-cpuinfo==9.0.0
+pytest==7.1.3
+pytest-benchmark==4.0.0
+tomli==2.0.1
+typing_extensions==4.10.0
+wrapt==1.16.0
+zipp==3.17.0
+-e opentelemetry-api
+-e propagator/opentelemetry-propagator-b3
diff --git a/tox.ini b/tox.ini
index 3d688944eba..2dae2d3163b 100644
--- a/tox.ini
+++ b/tox.ini
@@ -151,7 +151,7 @@ commands_pre =
 
   exporter-zipkin-json: pip install {toxinidir}/exporter/opentelemetry-exporter-zipkin-json
 
-  b3: pip install {toxinidir}/propagator/opentelemetry-propagator-b3
+  propagator-b3: pip install -r {toxinidir}/propagator/opentelemetry-propagator-b3/test-requirements.txt
 
   propagator-jaeger: pip install {toxinidir}/propagator/opentelemetry-propagator-jaeger
 
@@ -229,7 +229,7 @@ commands_pre =
   python -m pip install -e {toxinidir}/exporter/opentelemetry-exporter-zipkin-json[test]
   python -m pip install -e {toxinidir}/exporter/opentelemetry-exporter-zipkin-proto-http[test]
   python -m pip install -e {toxinidir}/exporter/opentelemetry-exporter-zipkin[test]
-  python -m pip install -e {toxinidir}/propagator/opentelemetry-propagator-b3[test]
+  pip install -r {toxinidir}/propagator/opentelemetry-propagator-b3/test-requirements.txt
   python -m pip install -e {toxinidir}/propagator/opentelemetry-propagator-jaeger[test]
   # Pin protobuf version due to lint failing on v3.20.0
   # https://github.com/protocolbuffers/protobuf/issues/9730