diff --git a/instrumentation/opentelemetry-instrumentation-grpc/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-grpc/test-requirements.txt
index 56d47af0df..9303dc27c0 100644
--- a/instrumentation/opentelemetry-instrumentation-grpc/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-grpc/test-requirements.txt
@@ -10,7 +10,6 @@ protobuf==3.20.3
 py==1.11.0
 py-cpuinfo==9.0.0
 pytest==7.1.3
-pytest-asyncio==0.23.5
 pytest-benchmark==4.0.0
 tomli==2.0.1
 typing_extensions==4.9.0
diff --git a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor.py b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor.py
index 21dbc44066..7ae1649149 100644
--- a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor.py
+++ b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor.py
@@ -11,24 +11,9 @@
 # WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
 # See the License for the specific language governing permissions and
 # limitations under the License.
-try:
-    from unittest import IsolatedAsyncioTestCase
-except ImportError:
-    # unittest.IsolatedAsyncioTestCase was introduced in Python 3.8. It's use
-    # simplifies the following tests. Without it, the amount of test code
-    # increases significantly, with most of the additional code handling
-    # the asyncio set up.
-    from unittest import TestCase
-
-    class IsolatedAsyncioTestCase(TestCase):
-        def run(self, result=None):
-            self.skipTest(
-                "This test requires Python 3.8 for unittest.IsolatedAsyncioTestCase"
-            )
-
+from unittest import IsolatedAsyncioTestCase
 
 import grpc
-import pytest
 
 import opentelemetry.instrumentation.grpc
 from opentelemetry import trace
@@ -65,7 +50,6 @@ async def intercept_unary_unary(
         return await continuation(client_call_details, request)
 
 
-@pytest.mark.asyncio
 class TestAioClientInterceptor(TestBase, IsolatedAsyncioTestCase):
     def setUp(self):
         super().setUp()
diff --git a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor_filter.py b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor_filter.py
index b8c408c6cf..2bd68fd492 100644
--- a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor_filter.py
+++ b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor_filter.py
@@ -11,27 +11,10 @@
 # WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
 # See the License for the specific language governing permissions and
 # limitations under the License.
-try:
-    from unittest import IsolatedAsyncioTestCase
-except ImportError:
-    # unittest.IsolatedAsyncioTestCase was introduced in Python 3.8. It's use
-    # simplifies the following tests. Without it, the amount of test code
-    # increases significantly, with most of the additional code handling
-    # the asyncio set up.
-    from unittest import TestCase
-
-    class IsolatedAsyncioTestCase(TestCase):
-        def run(self, result=None):
-            self.skipTest(
-                "This test requires Python 3.8 for unittest.IsolatedAsyncioTestCase"
-            )
-
-
 import os
-from unittest import mock
+from unittest import IsolatedAsyncioTestCase, mock
 
 import grpc
-import pytest
 
 from opentelemetry.instrumentation.grpc import (
     GrpcAioInstrumentorClient,
@@ -50,7 +33,6 @@ def run(self, result=None):
 from .protobuf import test_server_pb2_grpc  # pylint: disable=no-name-in-module
 
 
-@pytest.mark.asyncio
 class TestAioClientInterceptorFiltered(TestBase, IsolatedAsyncioTestCase):
     def setUp(self):
         super().setUp()
diff --git a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor_hooks.py b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor_hooks.py
index fe906b26c1..3069b30ae4 100644
--- a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor_hooks.py
+++ b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_client_interceptor_hooks.py
@@ -11,24 +11,9 @@
 # WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
 # See the License for the specific language governing permissions and
 # limitations under the License.
-try:
-    from unittest import IsolatedAsyncioTestCase
-except ImportError:
-    # unittest.IsolatedAsyncioTestCase was introduced in Python 3.8. It's use
-    # simplifies the following tests. Without it, the amount of test code
-    # increases significantly, with most of the additional code handling
-    # the asyncio set up.
-    from unittest import TestCase
-
-    class IsolatedAsyncioTestCase(TestCase):
-        def run(self, result=None):
-            self.skipTest(
-                "This test requires Python 3.8 for unittest.IsolatedAsyncioTestCase"
-            )
-
+from unittest import IsolatedAsyncioTestCase
 
 import grpc
-import pytest
 
 from opentelemetry.instrumentation.grpc import GrpcAioInstrumentorClient
 from opentelemetry.test.test_base import TestBase
@@ -54,7 +39,6 @@ def response_hook_with_exception(_span, _response):
     raise Exception()
 
 
-@pytest.mark.asyncio
 class TestAioClientInterceptorWithHooks(TestBase, IsolatedAsyncioTestCase):
     def setUp(self):
         super().setUp()
diff --git a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_server_interceptor.py b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_server_interceptor.py
index 7b31b085de..050f6f8d13 100644
--- a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_server_interceptor.py
+++ b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_server_interceptor.py
@@ -12,26 +12,10 @@
 # See the License for the specific language governing permissions and
 # limitations under the License.
 import asyncio
-
-try:
-    from unittest import IsolatedAsyncioTestCase
-except ImportError:
-    # unittest.IsolatedAsyncioTestCase was introduced in Python 3.8. It's use
-    # simplifies the following tests. Without it, the amount of test code
-    # increases significantly, with most of the additional code handling
-    # the asyncio set up.
-    from unittest import TestCase
-
-    class IsolatedAsyncioTestCase(TestCase):
-        def run(self, result=None):
-            self.skipTest(
-                "This test requires Python 3.8 for unittest.IsolatedAsyncioTestCase"
-            )
-
+from unittest import IsolatedAsyncioTestCase
 
 import grpc
 import grpc.aio
-import pytest
 
 import opentelemetry.instrumentation.grpc
 from opentelemetry import trace
@@ -97,7 +81,6 @@ async def run_with_test_server(
     return resp
 
 
-@pytest.mark.asyncio
 class TestOpenTelemetryAioServerInterceptor(TestBase, IsolatedAsyncioTestCase):
     async def test_instrumentor(self):
         """Check that automatic instrumentation configures the interceptor"""
diff --git a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_server_interceptor_filter.py b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_server_interceptor_filter.py
index 837d9c7618..34b755ced8 100644
--- a/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_server_interceptor_filter.py
+++ b/instrumentation/opentelemetry-instrumentation-grpc/tests/test_aio_server_interceptor_filter.py
@@ -11,25 +11,10 @@
 # WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
 # See the License for the specific language governing permissions and
 # limitations under the License.
-try:
-    from unittest import IsolatedAsyncioTestCase
-except ImportError:
-    # unittest.IsolatedAsyncioTestCase was introduced in Python 3.8. It's use
-    # simplifies the following tests. Without it, the amount of test code
-    # increases significantly, with most of the additional code handling
-    # the asyncio set up.
-    from unittest import TestCase
-
-    class IsolatedAsyncioTestCase(TestCase):
-        def run(self, result=None):
-            self.skipTest(
-                "This test requires Python 3.8 for unittest.IsolatedAsyncioTestCase"
-            )
-
+from unittest import IsolatedAsyncioTestCase
 
 import grpc
 import grpc.aio
-import pytest
 
 from opentelemetry import trace
 from opentelemetry.instrumentation.grpc import (
@@ -68,7 +53,6 @@ async def run_with_test_server(
     return resp
 
 
-@pytest.mark.asyncio
 class TestOpenTelemetryAioServerInterceptor(TestBase, IsolatedAsyncioTestCase):
     async def test_instrumentor(self):
         """Check that automatic instrumentation configures the interceptor"""
diff --git a/tox.ini b/tox.ini
index fecc9e5af7..a60aa813e7 100644
--- a/tox.ini
+++ b/tox.ini
@@ -374,7 +374,6 @@ deps =
   test: pytest-benchmark
   coverage: pytest
   coverage: pytest-cov
-  grpc: pytest-asyncio
 
   ; FIXME: add coverage testing
   ; FIXME: add mypy testing