diff --git a/CHANGELOG.md b/CHANGELOG.md
index 0373f30754..215221302d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -25,6 +25,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   ([#2266](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/2266))
 - `opentelemetry-instrumentation-elasticsearch` Don't send bulk request body as db statement
   ([#2355](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/2355))
+- AwsLambdaInstrumentor sets `cloud.account.id` span attribute
+  ([#2367](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/2367))
 
 ## Version 1.23.0/0.44b0 (2024-02-23)
 
diff --git a/instrumentation/opentelemetry-instrumentation-aws-lambda/src/opentelemetry/instrumentation/aws_lambda/__init__.py b/instrumentation/opentelemetry-instrumentation-aws-lambda/src/opentelemetry/instrumentation/aws_lambda/__init__.py
index 130622f8c8..3ec22d4c0e 100644
--- a/instrumentation/opentelemetry-instrumentation-aws-lambda/src/opentelemetry/instrumentation/aws_lambda/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-aws-lambda/src/opentelemetry/instrumentation/aws_lambda/__init__.py
@@ -354,6 +354,16 @@ def _instrumented_lambda_handler_call(  # noqa pylint: disable=too-many-branches
                     lambda_context.aws_request_id,
                 )
 
+                # NOTE: `cloud.account.id` can be parsed from the ARN as the fifth item when splitting on `:`
+                #
+                # See more:
+                # https://github.com/open-telemetry/semantic-conventions/blob/main/docs/faas/aws-lambda.md#all-triggers
+                account_id = lambda_context.invoked_function_arn.split(":")[4]
+                span.set_attribute(
+                    ResourceAttributes.CLOUD_ACCOUNT_ID,
+                    account_id,
+                )
+
             exception = None
             try:
                 result = call_wrapped(*args, **kwargs)
diff --git a/instrumentation/opentelemetry-instrumentation-aws-lambda/tests/test_aws_lambda_instrumentation_manual.py b/instrumentation/opentelemetry-instrumentation-aws-lambda/tests/test_aws_lambda_instrumentation_manual.py
index 2fa4aafee5..9bf6f47d7b 100644
--- a/instrumentation/opentelemetry-instrumentation-aws-lambda/tests/test_aws_lambda_instrumentation_manual.py
+++ b/instrumentation/opentelemetry-instrumentation-aws-lambda/tests/test_aws_lambda_instrumentation_manual.py
@@ -54,7 +54,7 @@ def __init__(self, aws_request_id, invoked_function_arn):
 
 MOCK_LAMBDA_CONTEXT = MockLambdaContext(
     aws_request_id="mock_aws_request_id",
-    invoked_function_arn="arn://mock-lambda-function-arn",
+    invoked_function_arn="arn:aws:lambda:us-east-1:123456:function:myfunction:myalias",
 )
 
 MOCK_XRAY_TRACE_ID = 0x5FB7331105E8BB83207FA31D4D9CDB4C
@@ -147,6 +147,11 @@ def test_active_tracing(self):
             {
                 ResourceAttributes.FAAS_ID: MOCK_LAMBDA_CONTEXT.invoked_function_arn,
                 SpanAttributes.FAAS_EXECUTION: MOCK_LAMBDA_CONTEXT.aws_request_id,
+                ResourceAttributes.CLOUD_ACCOUNT_ID: MOCK_LAMBDA_CONTEXT.invoked_function_arn.split(
+                    ":"
+                )[
+                    4
+                ],
             },
         )