diff --git a/instrumentation/opentelemetry-instrumentation-aiohttp-client/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-aiohttp-client/test-requirements.txt
index f02ffb216f..8f713687bd 100644
--- a/instrumentation/opentelemetry-instrumentation-aiohttp-client/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-aiohttp-client/test-requirements.txt
@@ -14,7 +14,7 @@ idna==3.7
 importlib-metadata==6.11.0
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.3
+Jinja2==3.1.4
 MarkupSafe==2.1.5
 multidict==6.0.5
 packaging==24.0
diff --git a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt
index fcedc7c695..fad2f5e2b0 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt
+++ b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt
@@ -5,7 +5,7 @@ Flask==2.1.3
 importlib-metadata==6.11.0
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.3
+Jinja2==3.1.4
 MarkupSafe==2.1.2
 packaging==24.0
 pluggy==1.5.0
diff --git a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt
index c6773ad2e9..919ee6d431 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt
+++ b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt
@@ -5,7 +5,7 @@ Flask==2.2.0
 importlib-metadata==6.11.0
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.3
+Jinja2==3.1.4
 MarkupSafe==2.1.2
 packaging==24.0
 pluggy==1.5.0
diff --git a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-2.txt b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-2.txt
index 98638a4ed6..16d91d2058 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-2.txt
+++ b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-2.txt
@@ -6,7 +6,7 @@ Flask==3.0.2
 importlib-metadata==6.11.0
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.3
+Jinja2==3.1.4
 MarkupSafe==2.1.2
 packaging==24.0
 pluggy==1.5.0
diff --git a/instrumentation/opentelemetry-instrumentation-jinja2/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-jinja2/test-requirements.txt
index 6461129bee..e547f9bd20 100644
--- a/instrumentation/opentelemetry-instrumentation-jinja2/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-jinja2/test-requirements.txt
@@ -2,7 +2,7 @@ asgiref==3.7.2
 Deprecated==1.2.14
 importlib-metadata==6.11.0
 iniconfig==2.0.0
-Jinja2==3.1.3
+Jinja2==3.1.4
 MarkupSafe==2.0.1
 packaging==24.0
 pluggy==1.5.0
diff --git a/instrumentation/opentelemetry-instrumentation-tornado/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-tornado/test-requirements.txt
index 121fdacf3c..a68f40e5d2 100644
--- a/instrumentation/opentelemetry-instrumentation-tornado/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-tornado/test-requirements.txt
@@ -10,7 +10,7 @@ idna==3.7
 importlib-metadata==6.11.0
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.3
+Jinja2==3.1.4
 MarkupSafe==2.1.5
 packaging==24.0
 pluggy==1.5.0