diff --git a/.github/workflows/ci-python.yml b/.github/workflows/ci-python.yml
index 6c777be1f5..0c8005f0cd 100644
--- a/.github/workflows/ci-python.yml
+++ b/.github/workflows/ci-python.yml
@@ -26,7 +26,7 @@ jobs:
 
     strategy:
       matrix:
-        python: ['3.8', '3.9', '3.10', '3.11']
+        python: ['3.8', '3.9', '3.10', '3.11', '3.12']
 
     steps:
       - name: Checkout this repo
diff --git a/collector/Makefile b/collector/Makefile
index c88283046f..c21148fdb0 100644
--- a/collector/Makefile
+++ b/collector/Makefile
@@ -47,12 +47,12 @@ package: build
 
 .PHONY: publish
 publish:
-	aws lambda publish-layer-version --layer-name $(LAYER_NAME) --zip-file fileb://$(BUILD_SPACE)/opentelemetry-collector-layer-$(GOARCH).zip --compatible-runtimes nodejs14.x nodejs16.x nodejs18.x java11 python3.8 python3.9 python3.10 python3.11 --query 'LayerVersionArn' --output text
+	aws lambda publish-layer-version --layer-name $(LAYER_NAME) --zip-file fileb://$(BUILD_SPACE)/opentelemetry-collector-layer-$(GOARCH).zip --compatible-runtimes nodejs14.x nodejs16.x nodejs18.x java11 python3.8 python3.9 python3.10 python3.11 python3.12 --query 'LayerVersionArn' --output text
 
 .PHONY: publish-layer
 publish-layer: package
 	@echo Publishing collector extension layer...
-	aws lambda publish-layer-version --layer-name $(LAYER_NAME) --zip-file fileb://$(BUILD_SPACE)/opentelemetry-collector-layer-$(GOARCH).zip --compatible-runtimes nodejs14.x nodejs16.x nodejs18.x java11 python3.8 python3.9 python3.10 python3.11 --query 'LayerVersionArn' --output text
+	aws lambda publish-layer-version --layer-name $(LAYER_NAME) --zip-file fileb://$(BUILD_SPACE)/opentelemetry-collector-layer-$(GOARCH).zip --compatible-runtimes nodejs14.x nodejs16.x nodejs18.x java11 python3.8 python3.9 python3.10 python3.11 python3.12 --query 'LayerVersionArn' --output text
 	@echo OpenTelemetry Collector layer published.
 
 .PHONY: set-otelcol-version
diff --git a/python/sample-apps/template.yml b/python/sample-apps/template.yml
index 4c0360fddf..7f7a1c3619 100644
--- a/python/sample-apps/template.yml
+++ b/python/sample-apps/template.yml
@@ -46,5 +46,6 @@ Resources:
         - python3.9
         - python3.10
         - python3.11
+        - python3.12
     Metadata:
       BuildMethod: makefile
diff --git a/python/src/otel/Dockerfile b/python/src/otel/Dockerfile
index f92e728a7a..7a8fe8f8a3 100644
--- a/python/src/otel/Dockerfile
+++ b/python/src/otel/Dockerfile
@@ -1,4 +1,4 @@
-ARG runtime=python3.11
+ARG runtime=python3.12
 
 FROM public.ecr.aws/sam/build-${runtime}
 
diff --git a/python/src/template.yml b/python/src/template.yml
index 717cae3b59..1b486f7d00 100644
--- a/python/src/template.yml
+++ b/python/src/template.yml
@@ -18,5 +18,6 @@ Resources:
         - python3.9
         - python3.10
         - python3.11
+        - python3.12
     Metadata:
       BuildMethod: makefile