diff --git a/plugins/node/opentelemetry-instrumentation-aws-sdk/.tav.yml b/plugins/node/opentelemetry-instrumentation-aws-sdk/.tav.yml
index b678e4b15c..0ad091fb0d 100644
--- a/plugins/node/opentelemetry-instrumentation-aws-sdk/.tav.yml
+++ b/plugins/node/opentelemetry-instrumentation-aws-sdk/.tav.yml
@@ -1,18 +1,18 @@
 "aws-sdk":
   # A small subset of releases in the range [2.308.0, 3) to reduce testing time.
-  versions: "2.308.0 || 2.548.0 || 2.785.0 || 2.1025.0 || 2.1265.0 || 2.1506.0 || >=2.1508.0"
+  versions: "2.308.0 || 2.556.0 || 2.801.0 || 2.1049.0 || 2.1297.0 || 2.1546.0 || >=2.1548.0"
   commands:
     - npm run test
 
 "@aws-sdk/client-s3":
   # A small subset of releases in the range [3.6.1, 4) to reduce testing time.
   # - 3.377.0 was a bad release (see issue #1828).
-  versions: "3.6.1 || 3.53.0 || 3.163.0 || 3.266.0 || 3.354.0 || 3.458.0 || >=3.462.0"
+  versions: "3.6.1 || 3.55.0 || 3.180.0 || 3.289.0 || 3.385.0 || 3.498.0 || >=3.503.1"
   commands:
     - npm run test
 
 "@aws-sdk/client-sqs":
   # A small subset of releases in the range [3.24.0, 4) to reduce testing time.
-  versions: "3.24.0 || 3.85.0 || 3.194.0 || 3.278.0 || 3.357.0 || 3.461.0 || >=3.462.0"
+  versions: "3.24.0 || 3.94.0 || 3.202.0 || 3.296.0 || 3.388.0 || 3.496.0 || >=3.503.1"
   commands:
     - npm run test
diff --git a/plugins/node/opentelemetry-instrumentation-aws-sdk/src/aws-sdk.ts b/plugins/node/opentelemetry-instrumentation-aws-sdk/src/aws-sdk.ts
index f07a0795ac..349d2ff388 100644
--- a/plugins/node/opentelemetry-instrumentation-aws-sdk/src/aws-sdk.ts
+++ b/plugins/node/opentelemetry-instrumentation-aws-sdk/src/aws-sdk.ts
@@ -56,6 +56,7 @@ import {
   normalizeV3Request,
   removeSuffixFromStringIfExists,
 } from './utils';
+import { propwrap } from './propwrap';
 import { RequestMetadata } from './services/ServiceExtension';
 import { SemanticAttributes } from '@opentelemetry/semantic-conventions';
 
@@ -112,19 +113,24 @@ export class AwsInstrumentation extends InstrumentationBase<any> {
       v3MiddlewareStackFileNewVersions,
     ]);
 
-    // patch for @smithy/middleware-stack for aws-sdk packages v3.363.0+
-    const v3SmithyMiddlewareStackFile = new InstrumentationNodeModuleFile(
-      '@smithy/middleware-stack/dist-cjs/MiddlewareStack.js',
-      ['>=1.0.1'],
-      this.patchV3ConstructStack.bind(this),
-      this.unpatchV3ConstructStack.bind(this)
-    );
+    // Patch for @smithy/middleware-stack for @aws-sdk/* packages v3.363.0+.
+    // As of @smithy/middleware-stack@2.1.0 `constructStack` is only available
+    // as a getter, so we cannot use `this._wrap()`.
+    const self = this;
     const v3SmithyMiddlewareStack = new InstrumentationNodeModuleDefinition(
       '@smithy/middleware-stack',
       ['>=2.0.0'],
-      undefined,
-      undefined,
-      [v3SmithyMiddlewareStackFile]
+      (moduleExports, moduleVersion) => {
+        const newExports = propwrap(
+          moduleExports,
+          'constructStack',
+          (orig: any) => {
+            self._diag.debug('propwrapping aws-sdk v3 constructStack');
+            return self._getV3ConstructStackPatch(moduleVersion, orig);
+          }
+        );
+        return newExports;
+      }
     );
 
     const v3SmithyClient = new InstrumentationNodeModuleDefinition<typeof AWS>(
diff --git a/plugins/node/opentelemetry-instrumentation-aws-sdk/src/propwrap.ts b/plugins/node/opentelemetry-instrumentation-aws-sdk/src/propwrap.ts
new file mode 100644
index 0000000000..ca59d52c54
--- /dev/null
+++ b/plugins/node/opentelemetry-instrumentation-aws-sdk/src/propwrap.ts
@@ -0,0 +1,155 @@
+/*
+ * Copyright The OpenTelemetry Authors
+ *
+ * Licensed under the Apache License, Version 2.0 (the "License");
+ * you may not use this file except in compliance with the License.
+ * You may obtain a copy of the License at
+ *
+ *      https://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing, software
+ * distributed under the License is distributed on an "AS IS" BASIS,
+ * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+ * See the License for the specific language governing permissions and
+ * limitations under the License.
+ */
+
+/*
+ * This block is derived from esbuild's bundling support.
+ * https://github.com/evanw/esbuild/blob/v0.14.42/internal/runtime/runtime.go#L22
+ *
+ * License:
+ * MIT License
+ *
+ * Copyright (c) 2020 Evan Wallace
+ *
+ * Permission is hereby granted, free of charge, to any person obtaining a copy
+ * of this software and associated documentation files (the "Software"), to deal
+ * in the Software without restriction, including without limitation the rights
+ * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
+ * copies of the Software, and to permit persons to whom the Software is
+ * furnished to do so, subject to the following conditions:
+ *
+ * The above copyright notice and this permission notice shall be included in all
+ * copies or substantial portions of the Software.
+ *
+ * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
+ * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
+ * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
+ * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
+ * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
+ * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
+ * SOFTWARE.
+ */
+const __defProp = Object.defineProperty;
+const __getOwnPropDesc = Object.getOwnPropertyDescriptor;
+const __hasOwnProp = Object.prototype.hasOwnProperty;
+const __getOwnPropNames = Object.getOwnPropertyNames;
+const __copyProps = (
+  to: any,
+  from: any,
+  except: string,
+  desc?: PropertyDescriptor | undefined
+) => {
+  if ((from && typeof from === 'object') || typeof from === 'function') {
+    for (const key of __getOwnPropNames(from)) {
+      if (!__hasOwnProp.call(to, key) && key !== except) {
+        __defProp(to, key, {
+          get: () => from[key] as any,
+          enumerable: !(desc = __getOwnPropDesc(from, key)) || desc.enumerable,
+        });
+      }
+    }
+  }
+  return to;
+};
+
+/**
+ * Return a new object that is a copy of `obj`, with its `subpath` property
+ * replaced with the return value of `wrapper(original)`.
+ *
+ * This is similar to shimmer (i.e. `InstrumentationBase.prototype._wrap`).
+ * However, it uses a different technique to support wrapping properties that
+ * are only available via a getter (i.e. their property descriptor is `.writable
+ * === false`).
+ *
+ * For example:
+ *    var os = propwrap(require('os'), 'platform', (orig) => {
+ *      return function wrappedPlatform () {
+ *        return orig().toUpperCase()
+ *      }
+ *    })
+ *    console.log(os.platform()) // => DARWIN
+ *
+ * The subpath can indicate a nested property. Each property in that subpath,
+ * except the last, must identify an *Object*.
+ *
+ * Limitations:
+ * - This doesn't handle possible Symbol properties on the copied object(s).
+ * - This cannot wrap a property of a function, because we cannot create a
+ *   copy of the function.
+ *
+ * @param {object} obj
+ * @param {string} subpath - The property subpath on `obj` to wrap. This may
+ *    point to a nested property by using a '.' to separate levels. For example:
+ *        var fs = wrap(fs, 'promises.sync', (orig) => { ... })
+ * @param {Function} wrapper - A function of the form `function (orig)`, where
+ *    `orig` is the original property value. This must synchronously return the
+ *    new property value.
+ * @returns {object} A new object with the wrapped property.
+ * @throws {TypeError} if the subpath points to a non-existant property, or if
+ *    any but the last subpath part points to a non-Object.
+ */
+export const propwrap = (obj: any, subpath: string, wrapper: Function): any => {
+  const parts = subpath.split('.');
+  const namespaces = [obj];
+  let namespace = obj;
+  let key;
+  let val;
+
+  // 1. Traverse the subpath parts to sanity check and get references to the
+  //    Objects that we will be copying.
+  for (let i = 0; i < parts.length; i++) {
+    key = parts[i];
+    val = namespace[key];
+    if (!val) {
+      throw new TypeError(
+        `cannot wrap "${subpath}": "<obj>.${parts
+          .slice(0, i)
+          .join('.')}" is ${typeof val}`
+      );
+    } else if (i < parts.length - 1) {
+      if (typeof val !== 'object') {
+        throw new TypeError(
+          `cannot wrap "${subpath}": "<obj>.${parts
+            .slice(0, i)
+            .join('.')}" is not an Object`
+        );
+      }
+      namespace = val;
+      namespaces.push(namespace);
+    }
+  }
+
+  // 2. Now work backwards, wrapping each namespace with a new object that has a
+  //    copy of all the properties, except the one that we've wrapped.
+  for (let i = parts.length - 1; i >= 0; i--) {
+    key = parts[i];
+    namespace = namespaces[i];
+    if (i === parts.length - 1) {
+      const orig = namespace[key];
+      val = wrapper(orig);
+    } else {
+      val = namespaces[i + 1];
+    }
+    const desc = __getOwnPropDesc(namespace, key);
+    const wrappedNamespace = __defProp({}, key, {
+      value: val,
+      enumerable: !desc || desc.enumerable,
+    });
+    __copyProps(wrappedNamespace, namespace, key);
+    namespaces[i] = wrappedNamespace;
+  }
+
+  return namespaces[0];
+};