diff --git a/instrumentation/spring/spring-boot-autoconfigure/build.gradle.kts b/instrumentation/spring/spring-boot-autoconfigure/build.gradle.kts
index a96d3962e447..004694694419 100644
--- a/instrumentation/spring/spring-boot-autoconfigure/build.gradle.kts
+++ b/instrumentation/spring/spring-boot-autoconfigure/build.gradle.kts
@@ -71,6 +71,8 @@ dependencies {
   }
   testImplementation("javax.servlet:javax.servlet-api:3.1.0")
   testImplementation("jakarta.servlet:jakarta.servlet-api:5.0.0")
+  testRuntimeOnly("com.h2database:h2:1.4.197")
+  testRuntimeOnly("io.r2dbc:r2dbc-h2:1.0.0.RELEASE")
 
   testImplementation(project(":testing-common"))
   testImplementation("io.opentelemetry:opentelemetry-sdk")
diff --git a/instrumentation/spring/spring-boot-autoconfigure/src/test/java/io/opentelemetry/instrumentation/spring/autoconfigure/instrumentation/r2dbc/R2DbcInstrumentationAutoConfigurationTest.java b/instrumentation/spring/spring-boot-autoconfigure/src/test/java/io/opentelemetry/instrumentation/spring/autoconfigure/instrumentation/r2dbc/R2DbcInstrumentationAutoConfigurationTest.java
new file mode 100644
index 000000000000..b7bc9a25b997
--- /dev/null
+++ b/instrumentation/spring/spring-boot-autoconfigure/src/test/java/io/opentelemetry/instrumentation/spring/autoconfigure/instrumentation/r2dbc/R2DbcInstrumentationAutoConfigurationTest.java
@@ -0,0 +1,63 @@
+/*
+ * Copyright The OpenTelemetry Authors
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+package io.opentelemetry.instrumentation.spring.autoconfigure.instrumentation.r2dbc;
+
+import io.opentelemetry.api.OpenTelemetry;
+import io.opentelemetry.instrumentation.testing.junit.LibraryInstrumentationExtension;
+import io.opentelemetry.sdk.autoconfigure.spi.ConfigProperties;
+import io.opentelemetry.sdk.autoconfigure.spi.internal.DefaultConfigProperties;
+import io.opentelemetry.semconv.incubating.DbIncubatingAttributes;
+import java.util.Collections;
+import org.junit.jupiter.api.Test;
+import org.junit.jupiter.api.extension.RegisterExtension;
+import org.springframework.boot.autoconfigure.AutoConfigurations;
+import org.springframework.boot.autoconfigure.r2dbc.R2dbcAutoConfiguration;
+import org.springframework.boot.test.context.runner.ApplicationContextRunner;
+import org.springframework.r2dbc.core.DatabaseClient;
+
+class R2DbcInstrumentationAutoConfigurationTest {
+
+  @RegisterExtension
+  static final LibraryInstrumentationExtension testing = LibraryInstrumentationExtension.create();
+
+  private final ApplicationContextRunner runner =
+      new ApplicationContextRunner()
+          .withBean(
+              ConfigProperties.class,
+              () -> DefaultConfigProperties.createFromMap(Collections.emptyMap()))
+          .withConfiguration(
+              AutoConfigurations.of(
+                  R2dbcInstrumentationAutoConfiguration.class, R2dbcAutoConfiguration.class))
+          .withBean("openTelemetry", OpenTelemetry.class, testing::getOpenTelemetry);
+
+  @Test
+  void statementSanitizerEnabledByDefault() {
+    runner.run(
+        context -> {
+          DatabaseClient client = context.getBean(DatabaseClient.class);
+          client
+              .sql(
+                  "CREATE TABLE IF NOT EXISTS player(id INT NOT NULL AUTO_INCREMENT, name VARCHAR(255), age INT, PRIMARY KEY (id))")
+              .fetch()
+              .all()
+              .blockLast();
+          client.sql("SELECT * FROM player WHERE id = 1").fetch().all().blockLast();
+          testing.waitAndAssertTraces(
+              trace ->
+                  trace.hasSpansSatisfyingExactly(
+                      span ->
+                          span.hasAttribute(
+                              DbIncubatingAttributes.DB_STATEMENT,
+                              "CREATE TABLE IF NOT EXISTS player(id INT NOT NULL AUTO_INCREMENT, name VARCHAR(?), age INT, PRIMARY KEY (id))")),
+              trace ->
+                  trace.hasSpansSatisfyingExactly(
+                      span ->
+                          span.hasAttribute(
+                              DbIncubatingAttributes.DB_STATEMENT,
+                              "SELECT * FROM player WHERE id = ?")));
+        });
+  }
+}