diff --git a/README.md b/README.md index 067a9572..3a9b6c3f 100644 --- a/README.md +++ b/README.md @@ -143,7 +143,7 @@ The final graph (after compression) containing one single variant is depicted be _____|\__,_|_| _|\___|\___|\__| Program: lancet (micro-assembly somatic variant caller) -Version: 1.0.6 (beta), April 15 2018 +Version: 1.0.7, July 16 2018 Contact: Giuseppe Narzisi Usage: lancet [options] --tumor --normal --ref --reg @@ -165,10 +165,10 @@ Optional --min-map-qual, -b : minimum read mapping quality in Phred-scale [default: 15] --max-as-xs-diff, -Z : maximum different between AS and XS alignments scores [default: 5] --tip-len, -l : max tip length [default: 11] - --cov-thr, -c : coverage threshold [default: 5] - --cov-ratio, -x : minimum coverage ratio [default: 0.01] + --cov-thr, -c : min coverage threshold used to select reference anchors from the De Bruijn graph [default: 5] + --cov-ratio, -x : minimum coverage ratio used to remove nodes from the De Bruijn graph [default: 0.01] + --low-cov, -d : low coverage threshold used to remove nodes from the De Bruijn graph [default: 1] --max-avg-cov, -u : maximum average coverage allowed per region [default: 10000] - --low-cov, -d : low coverage threshold [default: 1] --window-size, -w : window size of the region to assemble (in base-pairs) [default: 600] --padding, -P : left/right padding (in base-pairs) applied to the input genomic regions [default: 250] --dfs-limit, -F : limit dfs/bfs graph traversal search space [default: 1000000] @@ -197,9 +197,10 @@ Short Tandem Repeat parameters --dist-from-str, -D : distance (in bp) of variant from STR locus [default: 1] Flags - --active-region-off, -W : turn off active region module - --kmer-recovery, -R : turn on k-mer recovery (experimental) - --print-graph, -A : print graph (in .dot format) after every stage - --verbose, -v : be verbose - --more-verbose, -V : be more verbose + --primary-alignment-only, -I : only use primary alignments for variant calling + --active-region-off, -W : turn off active region module + --kmer-recovery, -R : turn on k-mer recovery (experimental) + --print-graph, -A : print graph (in .dot format) after every stage + --verbose, -v : be verbose + --more-verbose, -V : be more verbose ``` \ No newline at end of file