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The sample-sheet only contains normal-samples error #1740

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ashpsnair opened this issue Nov 25, 2024 · 1 comment
Open

The sample-sheet only contains normal-samples error #1740

ashpsnair opened this issue Nov 25, 2024 · 1 comment
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@ashpsnair
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Description of the bug

I have been processing tumor normal samples using sarek and I have results for 6/14 samples
The pipeline stopped and I tried re-running it but no luck. What I am trying now is to run just one sample and I am getting this error:

The sample-sheet only contains normal-samples, but the following tools, which were requested with "--tools", expect at least one tumor-sample : mutect2
Missing process or function Channel.empty([[]])

Please note that this sample was already processed using sarek and I have annotated vcf (in another directory), I was doing this exercise as a part of debugging.

There was a previous issue reported: #1260 (I tried the solutions there but it isnt working for me)

Command used and terminal output

My NF code:

nextflow run nf-core/sarek \
   -profile singularity \
   --use_annotation_cache_keys \
   --input path/to/my/pwd/samplesheet.csv \
   --outdir path/to/my/pwd/folder/YS-8-14/ \
   --tools mutect2,vep

This is my samplesheet:

patient,sex,status,sample,lane,fastq_1,fastq_2
patient01,XY,0,N01,lane_1,/home/project/11003581/Data/YS-analysis/raw_data/N01_1.fq.gz,/home/project/11003581/Data/YS-analysis/raw_data/N01_2.fq.gz
patient01,XY,1,T01,lane_1,/home/project/11003581/Data/YS-analysis/raw_data/T01_1.fq.gz,/home/project/11003581/Data/YS-analysis/raw_data/T01_2.fq.gz

Relevant files

This is my .nextflow.log
nextflow.log

System information

Nextflow version: 24.10.1 build 5930
I am running it in HPC- Linux
Version of nf-core/sarek: 3.4.4
container: singularity

@ashpsnair ashpsnair added the bug Something isn't working label Nov 25, 2024
@FriederikeHanssen
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Hey! Yes ths is a known issue with the latest nextflow release. We have fixed this on dev. In the meantime, using the previous stable release of nextflow should circumvent this: #1622

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