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I have been processing tumor normal samples using sarek and I have results for 6/14 samples
The pipeline stopped and I tried re-running it but no luck. What I am trying now is to run just one sample and I am getting this error:
The sample-sheet only contains normal-samples, but the following tools, which were requested with "--tools", expect at least one tumor-sample : mutect2
Missing process or function Channel.empty([[]])
Please note that this sample was already processed using sarek and I have annotated vcf (in another directory), I was doing this exercise as a part of debugging.
There was a previous issue reported: #1260 (I tried the solutions there but it isnt working for me)
Command used and terminal output
My NF code:nextflow run nf-core/sarek \ -profile singularity \ --use_annotation_cache_keys \ --input path/to/my/pwd/samplesheet.csv \ --outdir path/to/my/pwd/folder/YS-8-14/ \ --tools mutect2,vepThis is my samplesheet:patient,sex,status,sample,lane,fastq_1,fastq_2patient01,XY,0,N01,lane_1,/home/project/11003581/Data/YS-analysis/raw_data/N01_1.fq.gz,/home/project/11003581/Data/YS-analysis/raw_data/N01_2.fq.gzpatient01,XY,1,T01,lane_1,/home/project/11003581/Data/YS-analysis/raw_data/T01_1.fq.gz,/home/project/11003581/Data/YS-analysis/raw_data/T01_2.fq.gz
Hey! Yes ths is a known issue with the latest nextflow release. We have fixed this on dev. In the meantime, using the previous stable release of nextflow should circumvent this: #1622
Description of the bug
I have been processing tumor normal samples using sarek and I have results for 6/14 samples
The pipeline stopped and I tried re-running it but no luck. What I am trying now is to run just one sample and I am getting this error:
The sample-sheet only contains normal-samples, but the following tools, which were requested with "--tools", expect at least one tumor-sample : mutect2
Missing process or function Channel.empty([[]])
Please note that this sample was already processed using sarek and I have annotated vcf (in another directory), I was doing this exercise as a part of debugging.
There was a previous issue reported: #1260 (I tried the solutions there but it isnt working for me)
Command used and terminal output
Relevant files
This is my .nextflow.log
nextflow.log
System information
Nextflow version: 24.10.1 build 5930
I am running it in HPC- Linux
Version of nf-core/sarek: 3.4.4
container: singularity
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