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Add deepsomatic tool #1647
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Add deepsomatic tool #1647
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Warning Newer version of the nf-core template is available. Your pipeline is using an old version of the nf-core template: 3.0.2. For more documentation on how to update your pipeline, please see the nf-core documentation and Synchronisation documentation. |
I like where this is going. |
Fix Changelog
intervals_only = intervals.map { intervals, num_intervals -> [[], intervals]} | ||
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DEEPSOMATIC(cram_normal_tumor_intervals, intervals_only, fasta, fasta_fai, [ [ id:'null' ], [] ]) |
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How does this work with multiple samples? We need to ensure that each sample is run with each interval subfile and then properly merged.
@@ -112,7 +112,7 @@ | |||
"fa_icon": "fas fa-toolbox", | |||
"description": "Tools to use for duplicate marking, variant calling and/or for annotation.", | |||
"help_text": "Multiple tools separated with commas.\n\n**Variant Calling:**\n\nGermline variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: DeepVariant, FreeBayes, GATK HaplotypeCaller, mpileup, Sentieon Haplotyper, Strelka\n- Structural Variants: indexcov, Manta, TIDDIT\n- Copy-number: CNVKit\n\nTumor-only somatic variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: FreeBayes, mpileup, Mutect2, Lofreq, Strelka\n- Structural Variants: Manta, TIDDIT\n- Copy-number: CNVKit, ControlFREEC\n\nSomatic variant calling can currently only be performed with the following variant callers:\n- SNPs/Indels: FreeBayes, Mutect2, Strelka2\n- Structural variants: Manta, TIDDIT\n- Copy-Number: ASCAT, CNVKit, Control-FREEC\n- Microsatellite Instability: MSIsensorpro\n\n> **NB** Mutect2 for somatic variant calling cannot be combined with `--no_intervals`\n\n**Annotation:**\n \n- snpEff, VEP, merge (both consecutively), and bcftools annotate (needs `--bcftools_annotation`).\n\n> **NB** As Sarek will use bgzip and tabix to compress and index VCF files annotated, it expects VCF files to be sorted when starting from `--step annotate`.", |
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"help_text": "Multiple tools separated with commas.\n\n**Variant Calling:**\n\nGermline variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: DeepVariant, FreeBayes, GATK HaplotypeCaller, mpileup, Sentieon Haplotyper, Strelka\n- Structural Variants: indexcov, Manta, TIDDIT\n- Copy-number: CNVKit\n\nTumor-only somatic variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: FreeBayes, mpileup, Mutect2, Lofreq, Strelka\n- Structural Variants: Manta, TIDDIT\n- Copy-number: CNVKit, ControlFREEC\n\nSomatic variant calling can currently only be performed with the following variant callers:\n- SNPs/Indels: FreeBayes, Mutect2, Strelka2\n- Structural variants: Manta, TIDDIT\n- Copy-Number: ASCAT, CNVKit, Control-FREEC\n- Microsatellite Instability: MSIsensorpro\n\n> **NB** Mutect2 for somatic variant calling cannot be combined with `--no_intervals`\n\n**Annotation:**\n \n- snpEff, VEP, merge (both consecutively), and bcftools annotate (needs `--bcftools_annotation`).\n\n> **NB** As Sarek will use bgzip and tabix to compress and index VCF files annotated, it expects VCF files to be sorted when starting from `--step annotate`.", | |
"help_text": "Multiple tools separated with commas.\n\n**Variant Calling:**\n\nGermline variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: DeepVariant, FreeBayes, GATK HaplotypeCaller, mpileup, Sentieon Haplotyper, Strelka\n- Structural Variants: indexcov, Manta, TIDDIT\n- Copy-number: CNVKit\n\nTumor-only somatic variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: FreeBayes, mpileup, Mutect2, Lofreq, Strelka\n- Structural Variants: Manta, TIDDIT\n- Copy-number: CNVKit, ControlFREEC\n\nSomatic variant calling can currently only be performed with the following variant callers:\n- SNPs/Indels: DeepSomatic, FreeBayes, Mutect2, Strelka2\n- Structural variants: Manta, TIDDIT\n- Copy-Number: ASCAT, CNVKit, Control-FREEC\n- Microsatellite Instability: MSIsensorpro\n\n> **NB** Mutect2 for somatic variant calling cannot be combined with `--no_intervals`\n\n**Annotation:**\n \n- snpEff, VEP, merge (both consecutively), and bcftools annotate (needs `--bcftools_annotation`).\n\n> **NB** As Sarek will use bgzip and tabix to compress and index VCF files annotated, it expects VCF files to be sorted when starting from `--step annotate`.", |
"deepsomatic": { | ||
"branch": "master", | ||
"git_sha": "89e06f63c5ee7abe5d6910277aff8e83b00a8b50", | ||
"installed_by": ["modules"] | ||
}, | ||
"deepsomatic": { | ||
"branch": "master", | ||
"git_sha": "89e06f63c5ee7abe5d6910277aff8e83b00a8b50", | ||
"installed_by": ["modules"] | ||
}, | ||
"deepvariant": { |
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"deepsomatic": { | |
"branch": "master", | |
"git_sha": "89e06f63c5ee7abe5d6910277aff8e83b00a8b50", | |
"installed_by": ["modules"] | |
}, | |
"deepsomatic": { | |
"branch": "master", | |
"git_sha": "89e06f63c5ee7abe5d6910277aff8e83b00a8b50", | |
"installed_by": ["modules"] | |
}, | |
"deepvariant": { | |
"deepsomatic": { | |
"branch": "master", | |
"git_sha": "89e06f63c5ee7abe5d6910277aff8e83b00a8b50", | |
"installed_by": ["modules"] | |
}, | |
"deepvariant/rundeepvariant": { |
minor issues with managing merge conflicts, I made suggestions |
This is a first version of PR to add deepsomatic capabilities into sarek pipeline. Currently this PR contains code changes only (without tests and most of metadata) and created to gather the initial feedback.
PR checklist
nf-core lint
).nextflow run . -profile test,docker --outdir <OUTDIR>
).nextflow run . -profile debug,test,docker --outdir <OUTDIR>
).docs/usage.md
is updated.docs/output.md
is updated.CHANGELOG.md
is updated.README.md
is updated (including new tool citations and authors/contributors).