diff --git a/CHANGELOG.md b/CHANGELOG.md
index 6e259fe49..dfba156d1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -14,14 +14,15 @@ and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0.
 - Made MultiQC crash behaviour stricter when dealing with large datasets, as reported by @ashildv
 - [#652](https://github.com/nf-core/eager/issues/652) Added note to documentation that when using `--skip_collapse` this will use _paired-end_ alignment mode with mappers when using PE data
 - [#626](https://github.com/nf-core/eager/issues/626) - Add additional checks to ensure pipeline will give useful error if cells of a TSV column are empty
-- Added note to documentation that when using `--skip_collapse` this will use _paired-end_ alignment mode with mappers when using PE data.  `
-- [#673](https://github.com/nf-core/eager/pull/673) Fix Kraken database loading when loading from directory instead of compressed file.
+- Added note to documentation that when using `--skip_collapse` this will use _paired-end_ alignment mode with mappers when using PE data
+- [#673](https://github.com/nf-core/eager/pull/673) Fix Kraken database loading when loading from directory instead of compressed file
 - [#688](https://github.com/nf-core/eager/issues/668) - Allow pipeline to complete, even if Qualimap crashes due to an empty or corrupt BAM file for one sample/library
-- [#683](https://github.com/nf-core/eager/pull/683) - Sets `--igenomes_ignore` to true by default, as rarely used by users currently and makes resolving configs less complex.
-- Added exit code `140` to re-tryable exit code list to account for certain scheduler wall-time limit fails.
+- [#683](https://github.com/nf-core/eager/pull/683) - Sets `--igenomes_ignore` to true by default, as rarely used by users currently and makes resolving configs less complex
+- Added exit code `140` to re-tryable exit code list to account for certain scheduler wall-time limit fails
 - [672](https://github.com/nf-core/eager/issues/672) - Removed java parameter from picard tools which could cause memory issues
 - [679](https://github.com/nf-core/eager/issues/679) - Refactor within-process bash conditions to groovy/nextflow, due to incompatibility with some servers environments
 - [#690](https://github.com/nf-core/eager/pull/690) - Fixed ANGSD output mode for beagle by setting `-doMajorMinor 1` as default in that case
+- [#693](https://github.com/nf-core/eager/issues/693) - Fixed broken TSV input validation for the Colour Chemistry column
 
 ### `Dependencies`
 
diff --git a/main.nf b/main.nf
index 63894bc12..f52931874 100644
--- a/main.nf
+++ b/main.nf
@@ -3598,7 +3598,7 @@ def extract_data(tsvFile) {
             if (!udg.matches('none') && !udg.matches('half') && !udg.matches('full')) exit 1, "[nf-core/eager] error: UDG treatment can only be 'none', 'half' or 'full'. See '--help' flag and documentation under 'running the pipeline' for more information. You have '${udg}'"
 
             // Check valid colour chemistry
-            if (!colour == 2 && !colour == 4) exit 1, "[nf-core/eager] error: Colour chemistry in TSV can either be 2 (e.g. NextSeq/NovaSeq) or 4 (e.g. HiSeq/MiSeq)"
+            if (!colour.matches('2') && !colour.matches('4')) exit 1, "[nf-core/eager] error: Colour chemistry in TSV can either be 2 (e.g. NextSeq/NovaSeq) or 4 (e.g. HiSeq/MiSeq)"
 
             //  Ensure that we do not accept incompatible chemistry setup
             if (!seqtype.matches('PE') && !seqtype.matches('SE')) exit 1, "[nf-core/eager] error:  SeqType for one or more rows in TSV is neither SE nor PE! see '--help' flag and documentation under 'running the pipeline' for more information. You have: '${seqtype}'"