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Merge branch 'dev' into consensus-pr
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weber8thomas authored Nov 21, 2024
2 parents a9ed8c4 + b350380 commit c3e4165
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4 changes: 4 additions & 0 deletions CHANGELOG.md
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Expand Up @@ -7,6 +7,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0

### `Added`

- [#804](https://github.com/nf-core/ampliseq/pull/804) - Added version 10 of Unite as parameter for `--dada_ref_taxonomy` (issue [#768](https://github.com/nf-core/ampliseq/issues/768))
- [#803](https://github.com/nf-core/ampliseq/pull/803) - New parameters introduced related to `--mergepairs_strategy`. These parameters would only be effective if `--mergepairs_strategy consensus` is set.

| **Parameter** | **Description** | **Default Value** |
Expand All @@ -17,13 +18,16 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
| **mergepairs_consensus_minoverlap** | The minimum number of overlapping base pairs required to merge forward and reverse reads. | 12 |
| **mergepairs_consensus_maxmismatch** | The maximum number of mismatches allowed within the overlapping region for merging reads. | 0 |
| **mergepairs_consensus_percentile_cutoff** | The percentile cutoff determining the minimum observed overlap in the dataset. | 0.001 |
=======

### `Changed`

- [#803](https://github.com/nf-core/ampliseq/pull/803) - Changed DADA2_DENOISING : `--concatenate_reads` renaming to `--mergepairs_strategy` ; support new method named "consensus" by setting `--mergepairs_strategy consensus` ; changed options of `--mergepairs_strategy` from TRUE/FALSE (boolean) to ["merge", "concatenate", "consensus"].

### `Fixed`

- [#800](https://github.com/nf-core/ampliseq/pull/800) - Fixed SH files for UNITE9.0, they were missing some entries due to a bug caused by API update in PlutoF

### `Dependencies`

- [#797](https://github.com/nf-core/ampliseq/pull/797) - Update QIIME2
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40 changes: 28 additions & 12 deletions conf/ref_databases.config
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Expand Up @@ -199,20 +199,28 @@ params {
dbversion = "SILVA v132 (https://zenodo.org/record/1172783)"
}
'unite-fungi' {
title = "UNITE general FASTA release for Fungi - Version 9.0"
file = [ "https://s3.hpc.ut.ee/plutof-public/original/fa1038da-d18d-46b7-88a9-c21bcf38c43d.tgz" ]
citation = "Abarenkov, Kessy; Zirk, Allan; Piirmann, Timo; Pöhönen, Raivo; Ivanov, Filipp; Nilsson, R. Henrik; Kõljalg, Urmas (2023): UNITE general FASTA release for Fungi. Version 18.07.2023. UNITE Community. https://doi.org/10.15156/BIO/2938067"
title = "UNITE general FASTA release for Fungi - Version 10.0"
file = [ "https://s3.hpc.ut.ee/plutof-public/original/d18aa648-3f4c-4f46-84d4-c8c5d48439ba.tgz" ]
citation = "Abarenkov, Kessy; Zirk, Allan; Piirmann, Timo; Pöhönen, Raivo; Ivanov, Filipp; Nilsson, R. Henrik; Kõljalg, Urmas (2024): UNITE general FASTA release for Fungi. Version 04.04.2024. UNITE Community. https://doi.org/10.15156/BIO/2959332"
fmtscript = "taxref_reformat_unite.sh"
dbversion = "UNITE-fungi v9.0 (https://doi.org/10.15156/BIO/2938067)"
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/40788767", "https://figshare.scilifelab.se/ndownloader/files/40788770"]
dbversion = "UNITE-fungi v10.0 (https://doi.org/10.15156/BIO/2959332)"
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/50595459", "https://figshare.scilifelab.se/ndownloader/files/50595462"]
}
'unite-fungi=10.0' {
title = "UNITE general FASTA release for Fungi - Version 10.0"
file = [ "https://s3.hpc.ut.ee/plutof-public/original/d18aa648-3f4c-4f46-84d4-c8c5d48439ba.tgz" ]
citation = "Abarenkov, Kessy; Zirk, Allan; Piirmann, Timo; Pöhönen, Raivo; Ivanov, Filipp; Nilsson, R. Henrik; Kõljalg, Urmas (2024): UNITE general FASTA release for Fungi. Version 04.04.2024. UNITE Community. https://doi.org/10.15156/BIO/2959332"
fmtscript = "taxref_reformat_unite.sh"
dbversion = "UNITE-fungi v10.0 (https://doi.org/10.15156/BIO/2959332)"
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/50595459", "https://figshare.scilifelab.se/ndownloader/files/50595462"]
}
'unite-fungi=9.0' {
title = "UNITE general FASTA release for Fungi - Version 9.0"
file = [ "https://s3.hpc.ut.ee/plutof-public/original/fa1038da-d18d-46b7-88a9-c21bcf38c43d.tgz" ]
citation = "Abarenkov, Kessy; Zirk, Allan; Piirmann, Timo; Pöhönen, Raivo; Ivanov, Filipp; Nilsson, R. Henrik; Kõljalg, Urmas (2023): UNITE general FASTA release for Fungi. Version 18.07.2023. UNITE Community. https://doi.org/10.15156/BIO/2938067"
fmtscript = "taxref_reformat_unite.sh"
dbversion = "UNITE-fungi v9.0 (https://doi.org/10.15156/BIO/2938067)"
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/40788767", "https://figshare.scilifelab.se/ndownloader/files/40788770"]
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/50055762", "https://figshare.scilifelab.se/ndownloader/files/50055765"]
}
'unite-fungi=8.3' {
title = "UNITE general FASTA release for Fungi - Version 8.3"
Expand All @@ -231,20 +239,28 @@ params {
shfile = [ "https://scilifelab.figshare.com/ndownloader/files/34497971", "https://scilifelab.figshare.com/ndownloader/files/34497974"]
}
'unite-alleuk' {
title = "UNITE general FASTA release for eukaryotes - Version 9.0"
file = [ "https://s3.hpc.ut.ee/plutof-public/original/e318f5fd-1ef4-40fd-9e77-1b94d91b3858.tgz" ]
citation = "Abarenkov, Kessy; Zirk, Allan; Piirmann, Timo; Pöhönen, Raivo; Ivanov, Filipp; Nilsson, R. Henrik; Kõljalg, Urmas (2023): UNITE general FASTA release for eukaryotes. Version 18.07.2023. UNITE Community. https://doi.org/10.15156/BIO/2938069"
title = "UNITE general FASTA release for eukaryotes - Version 10.0"
file = [ "https://s3.hpc.ut.ee/plutof-public/original/1dda2021-4893-4f2f-b50e-87bfea795267.tgz" ]
citation = "Abarenkov, Kessy; Zirk, Allan; Piirmann, Timo; Pöhönen, Raivo; Ivanov, Filipp; Nilsson, R. Henrik; Kõljalg, Urmas (2024): UNITE general FASTA release for eukaryotes. Version 04.04.2024. UNITE Community. https://doi.org/10.15156/BIO/2959334"
fmtscript = "taxref_reformat_unite.sh"
dbversion = "UNITE-alleuk v9.0 (https://doi.org/10.15156/BIO/2938069)"
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/40788773", "https://figshare.scilifelab.se/ndownloader/files/40788776"]
dbversion = "UNITE-alleuk v10.0 (https://doi.org/10.15156/BIO/2959334)"
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/50595465", "https://figshare.scilifelab.se/ndownloader/files/50595471"]
}
'unite-alleuk=10.0' {
title = "UNITE general FASTA release for eukaryotes - Version 10.0"
file = [ "https://s3.hpc.ut.ee/plutof-public/original/1dda2021-4893-4f2f-b50e-87bfea795267.tgz" ]
citation = "Abarenkov, Kessy; Zirk, Allan; Piirmann, Timo; Pöhönen, Raivo; Ivanov, Filipp; Nilsson, R. Henrik; Kõljalg, Urmas (2024): UNITE general FASTA release for eukaryotes. Version 04.04.2024. UNITE Community. https://doi.org/10.15156/BIO/2959334"
fmtscript = "taxref_reformat_unite.sh"
dbversion = "UNITE-alleuk v10.0 (https://doi.org/10.15156/BIO/2959334)"
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/50595465", "https://figshare.scilifelab.se/ndownloader/files/50595471"]
}
'unite-alleuk=9.0' {
title = "UNITE general FASTA release for eukaryotes - Version 9.0"
file = [ "https://s3.hpc.ut.ee/plutof-public/original/e318f5fd-1ef4-40fd-9e77-1b94d91b3858.tgz" ]
citation = "Abarenkov, Kessy; Zirk, Allan; Piirmann, Timo; Pöhönen, Raivo; Ivanov, Filipp; Nilsson, R. Henrik; Kõljalg, Urmas (2023): UNITE general FASTA release for eukaryotes. Version 18.07.2023. UNITE Community. https://doi.org/10.15156/BIO/2938069"
fmtscript = "taxref_reformat_unite.sh"
dbversion = "UNITE-alleuk v9.0 (https://doi.org/10.15156/BIO/2938069)"
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/40788773", "https://figshare.scilifelab.se/ndownloader/files/40788776"]
shfile = [ "https://figshare.scilifelab.se/ndownloader/files/50055768", "https://figshare.scilifelab.se/ndownloader/files/50055771"]
}
'unite-alleuk=8.3' {
title = "UNITE general FASTA release for eukaryotes - Version 8.3"
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2 changes: 2 additions & 0 deletions nextflow_schema.json
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Expand Up @@ -428,10 +428,12 @@
"silva=132",
"silva=138",
"unite-alleuk",
"unite-alleuk=10.0",
"unite-alleuk=9.0",
"unite-alleuk=8.3",
"unite-alleuk=8.2",
"unite-fungi",
"unite-fungi=10.0",
"unite-fungi=9.0",
"unite-fungi=8.3",
"unite-fungi=8.2",
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