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I am trying to simulate a set of HiFi reads 10x coverage of Pea Aphid genome. I am getting this in the error script:
rm: cannot remove `Sim-it-master/Output/PeaA/Long_reads_to_merge_PeaA_NW_021758228.1_Acyrthosiphon_pisum_isolate_AL4f_unplaced_genomic_scaffold,_pea_aphid_22Mar2018_4r6ur_Scaffold_10000': No such file or directory
rm: cannot remove `Sim-it-master/Output/PeaA/Long_reads_to_merge_PeaA_NW_021758229.1_Acyrthosiphon_pisum_isolate_AL4f_unplaced_genomic_scaffold,_pea_aphid_22Mar2018_4r6ur_Scaffold_10001': No such file or directory
rm: cannot remove `Sim-it-master/Output/PeaA/Long_reads_to_merge_PeaA_NW_021758230.1_Acyrthosiphon_pisum_isolate_AL4f_unplaced_genomic_scaffold,_pea_aphid_22Mar2018_4r6ur_Scaffold_10002': No such file or directory
Can you please help me.
This is my config.txt:
Project:
Project name = PeaA
Reference sequence = PeaAphid_Genome.fa
Replace ambiguous nts(N) =
Max threads = 16
Sorry for the late response
It is probably too late now, but I uploaded a new version that should have resolved your problem.
The read ids of your assembly file were too long to be used as a file name
I am trying to simulate a set of HiFi reads 10x coverage of Pea Aphid genome. I am getting this in the error script:
rm: cannot remove `Sim-it-master/Output/PeaA/Long_reads_to_merge_PeaA_NW_021758228.1_Acyrthosiphon_pisum_isolate_AL4f_unplaced_genomic_scaffold,_pea_aphid_22Mar2018_4r6ur_Scaffold_10000': No such file or directory
rm: cannot remove `Sim-it-master/Output/PeaA/Long_reads_to_merge_PeaA_NW_021758229.1_Acyrthosiphon_pisum_isolate_AL4f_unplaced_genomic_scaffold,_pea_aphid_22Mar2018_4r6ur_Scaffold_10001': No such file or directory
rm: cannot remove `Sim-it-master/Output/PeaA/Long_reads_to_merge_PeaA_NW_021758230.1_Acyrthosiphon_pisum_isolate_AL4f_unplaced_genomic_scaffold,_pea_aphid_22Mar2018_4r6ur_Scaffold_10002': No such file or directory
Can you please help me.
This is my config.txt:
Project:
Project name = PeaA
Reference sequence = PeaAphid_Genome.fa
Replace ambiguous nts(N) =
Max threads = 16
Structural variation:
VCF input =
Foreign sequences =
Deletions = 0
Length (bp) = 30-150000
Insertions = 0
Length (bp) = 30-100000
Tandem duplications = 0
Length (bp) = 50-10000
Copies = 1-20
Inversions = 0
Length (bp) = 150-1300000
Complex substitutions = 0
Length (bp) = 30-100000
Inverted duplications = 0
Length (bp) = 150-350000
Heterozygosity = 60%
Long Read simulation:
Sequencing depth = 10
Median length = 10000
Length range = 10000-15000
Accuracy = 99.9%
Error profile = error_profile_PB_Sequel_CCS_hifi.txt
This is my job script to run:
perl Sim-it1.3.4.pl -c t_config.txt -o Sim-it-master/Output/PeaA/
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