ShortSequence: Sequence is too long

[sleepvet]

I combined paired fq files into a fa file through " fq2fa --merge --filter 10.1.fa 10.2.fa 10.fa", then got the error hit when I run command "idba_ud -r 10.fa -o ../01assembly/".
the sequences I used is 150-300 bp.
the 10.fa file looks good.
the error hit is:terminate called after throwing an instance of 'std::logic_error'
what(): ShortSequence: Sequence is too long.
Aborted (core dumped)
I donot know what is wrong, could you please help me?

[sleepvet]

I assumed I should use fq files in fq2fa, then I run "" fq2fa --merge --filter 10.1.fq 10.2.fq 10.fa".Still, the fa file merged is not qualified.

[mrxuzj]

I combined paired fq files into a fa file through " fq2fa --merge --filter 10.1.fa 10.2.fa 10.fa", then got the error hit when I run command "idba_ud -r 10.fa -o ../01assembly/". the sequences I used is 150-300 bp. the 10.fa file looks good. the error hit is:terminate called after throwing an instance of 'std::logic_error' what(): ShortSequence: Sequence is too long. Aborted (core dumped) I donot know what is wrong, could you please help me?

same error, have you solved this problem？

[gingerbuffy]

I also got the same error and I followed the solution posted by akinori@jp in https://www.seqanswers.com/forum/bioinformatics/bioinformatics-aa/24625-250bp-reads-in-idba_ud and it works for me.

[mrxuzj]

您的邮件已收到，谢谢