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Error with erroneous sequence data #11

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Rhynchites opened this issue Aug 17, 2020 · 6 comments
Closed

Error with erroneous sequence data #11

Rhynchites opened this issue Aug 17, 2020 · 6 comments

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@Rhynchites
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When searching for SSRs in a large fasta file (with more than 250 000 sequences) I get the following error:

image

This is apparently triggered by erroneous sequence data, so far I traced back two sequences that did this, see attached files.

NODE_12838_length_70_cov_23.79.txt
NODE_42629_length_65_cov_2.692.txt

@mong222
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mong222 commented Aug 18, 2020

I also have the same error.
Could you tell me how to remove the erroneous data?

@Rhynchites
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Hi, Unfortunately I haven't found a way to remove the problematic sequence data in an efficient way yet.
It took me more than 2 hours to find those two sequences triggering the error.

It would be great if there was an option to ignore/skip those sequences, without having a crash of Krait.

@lmdu
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lmdu commented Aug 18, 2020

Thank you for reporting this bug. I will fix it as soon as possible!

@lmdu
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lmdu commented Aug 19, 2020

A new version was released to fix this bug. It was caused by sequence without G or C base, when counting the bases.

@mong222
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mong222 commented Aug 20, 2020

I tried the new version, but it crashed without errors when searching for SSRs in a large fasta file obtained from NCBI (more than 300 000 sequences).

@Rhynchites
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The new version of Krait ran my file without problems this time. Thanks for fixing this!

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3 participants