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Welcome to the DIALOGUE!

DIALOGUE is a dimensionality reduction method that uses cross-cell-type associations to identify multicellular programs (MCPs) and map the cell transcriptome as a function of its environment. Given single-cell data, it combines penalized matrix decomposition with multilevel modeling to identify generalizable MCPs and examines their association with specific phenotypes of interest.

Requirements

• R (tested in R version 3.4.0).
• R libraries: lme4, lmerTest, PMA, plyr, matrixStats, psych, stringi, RColorBrewer, unikn, reshape2, ggplot2

Quick start

To install you can either use devtools::install_github("DIALOGUE",your_user_name) or just download tha package and use devtools::install("DIALOGUE")

Here you can find simple step-by-step examples on existing data and new data.

Citation

Jerby-Arnon and Regev Mapping multicellular programs from single-cell transcriptomes.