diff --git a/pathogenprofiler/cli.py b/pathogenprofiler/cli.py
index 9110161..1fad0b6 100644
--- a/pathogenprofiler/cli.py
+++ b/pathogenprofiler/cli.py
@@ -10,7 +10,7 @@
 from .models import Variant, DrVariant, Gene, DrGene, SpeciesPrediction, Species, BarcodeResult
 from .mutation_db import MutationDB
 from .vcf import Vcf
-from .sanity import check_bam_for_rg, check_vcf_chrom_match
+from .sanity import check_bam_for_rg, check_vcf_chrom_match, check_bam_chrom_match
 
 def get_variant_filters(args):
     filters = {}
@@ -203,7 +203,7 @@ def run_profiler(args) -> List[Union[Variant,DrVariant,Gene,DrGene]]:
             tmp_vcf_file = f"{args.files_prefix}.tmp.vcf.gz"
             run_cmd(f"bcftools view {args.vcf} | modify_lofreq_vcf.py --sample {args.prefix} | bcftools view -Oz -o {tmp_vcf_file}")
             args.vcf = tmp_vcf_file
-    # check_vcf_chrom_match(args.vcf,args.conf["ref"])
+    check_vcf_chrom_match(args.vcf,args.conf["ref"])
     annotated_variants = vcf_profiler(args)
 
     return annotated_variants
@@ -262,6 +262,7 @@ def get_bam_file(args):
         bam_file = bam_obj.bam_file
     else:
         check_bam_for_rg(args.bam)
+        check_bam_chrom_match(args.bam,args.conf["ref"])
         bam_file = args.bam
 
     return bam_file
diff --git a/pathogenprofiler/sanity.py b/pathogenprofiler/sanity.py
index d8a495a..cfa9f27 100644
--- a/pathogenprofiler/sanity.py
+++ b/pathogenprofiler/sanity.py
@@ -17,3 +17,13 @@ def check_vcf_chrom_match(vcf_file, ref_file) -> None:
     for chrom in vcf.header.contigs:
         if chrom not in ref.references:
             raise Exception(f"Chromosome {chrom} in VCF file {vcf_file} not found in reference file {ref_file}.")
+
+def check_bam_chrom_match(bam_file, ref_file) -> None:
+    """
+    Check if the chromosomes in the BAM file match the reference file.
+    """
+    bam = pysam.AlignmentFile(bam_file, "rb")
+    ref = pysam.FastaFile(ref_file)
+    for chrom in bam.references:
+        if chrom not in ref.references:
+            raise Exception(f"Chromosome {chrom} in BAM file {bam_file} not found in reference file {ref_file}.")
\ No newline at end of file