diff --git a/.changeset/eleven-islands-jump.md b/.changeset/eleven-islands-jump.md
new file mode 100644
index 00000000..622b3632
--- /dev/null
+++ b/.changeset/eleven-islands-jump.md
@@ -0,0 +1,5 @@
+---
+'@hono/tsyringe': major
+---
+
+add tsyringe middleware support
diff --git a/.github/workflows/ci-tsyringe.yml b/.github/workflows/ci-tsyringe.yml
new file mode 100644
index 00000000..76ff993a
--- /dev/null
+++ b/.github/workflows/ci-tsyringe.yml
@@ -0,0 +1,25 @@
+name: ci-tsyringe
+on:
+  push:
+    branches: [main]
+    paths:
+      - 'packages/tsyringe/**'
+  pull_request:
+    branches: ['*']
+    paths:
+      - 'packages/tsyringe/**'
+
+jobs:
+  ci:
+    runs-on: ubuntu-latest
+    defaults:
+      run:
+        working-directory: ./packages/tsyringe
+    steps:
+      - uses: actions/checkout@v4
+      - uses: actions/setup-node@v4
+        with:
+          node-version: 20.x
+      - run: yarn install --frozen-lockfile
+      - run: yarn build
+      - run: yarn test
diff --git a/packages/tsyringe/README.md b/packages/tsyringe/README.md
new file mode 100644
index 00000000..1c8fcaf6
--- /dev/null
+++ b/packages/tsyringe/README.md
@@ -0,0 +1,57 @@
+# tsyringe middleware for Hono
+
+The [tsyringe](https://github.com/microsoft/tsyringe) middleware provides a way to use dependency injection in [Hono](https://hono.dev/).
+
+## Usage
+
+```ts
+import "reflect-metadata" // tsyringe requires reflect-metadata or polyfill
+import { container, inject, injectable } from 'tsyringe'
+import { tsyringe } from '@hono/tsyringe'
+import { Hono } from 'hono'
+
+@injectable()
+class Hello {
+  constructor(@inject('name') private name: string) {}
+
+  greet() {
+    return `Hello, ${this.name}!`
+  }
+}
+
+const app = new Hono()
+
+app.use('*', tsyringe((container) => {
+    container.register('name', { useValue: 'world' })
+}))
+
+app.get('/', (c) => {
+    const hello = container.resolve(Hello)
+    return c.text(hello.greet())
+})
+
+export default app
+```
+
+### With providers
+
+```ts
+const app = new Hono()
+
+app.use('/tenant/:name/*', async (c, next) => {
+    await tsyringe((container) => {
+        // Allowing to inject `c.var` or `c.req.param` in the providers
+        const tenantName = c.req.param('name')
+
+        container.register(Config, { useFactory: () => new Config(tenantName) })
+    })(c, next)
+})
+```
+
+## Author
+
+Aotokitsuruya <https://github.com/elct9620>
+
+## License
+
+MIT
diff --git a/packages/tsyringe/package.json b/packages/tsyringe/package.json
new file mode 100644
index 00000000..dc05efe6
--- /dev/null
+++ b/packages/tsyringe/package.json
@@ -0,0 +1,51 @@
+{
+  "name": "@hono/tsyringe",
+  "version": "0.1.0",
+  "description": "The tsyringe dependency injection middleware for Hono",
+  "type": "module",
+  "module": "dist/index.js",
+  "types": "dist/index.d.ts",
+  "files": [
+    "dist"
+  ],
+  "scripts": {
+    "test": "vitest --run",
+    "build": "tsup ./src/index.ts --format esm,cjs --dts",
+    "publint": "publint",
+    "release": "yarn build && yarn test && yarn publint && yarn publish"
+  },
+  "exports": {
+    ".": {
+      "import": {
+        "types": "./dist/index.d.ts",
+        "default": "./dist/index.js"
+      },
+      "require": {
+        "types": "./dist/index.d.cts",
+        "default": "./dist/index.cjs"
+      }
+    }
+  },
+  "license": "MIT",
+  "publishConfig": {
+    "registry": "https://registry.npmjs.org",
+    "access": "public"
+  },
+  "repository": {
+    "type": "git",
+    "url": "https://github.com/honojs/middleware.git"
+  },
+  "homepage": "https://github.com/honojs/middleware",
+  "peerDependencies": {
+    "hono": ">=4.*",
+    "tsyringe": ">=4.*"
+  },
+  "devDependencies": {
+    "hono": "^4.4.12",
+    "prettier": "^3.3.3",
+    "reflect-metadata": "^0.2.2",
+    "tsup": "^8.1.0",
+    "tsyringe": "^4.8.0",
+    "vitest": "^1.6.0"
+  }
+}
diff --git a/packages/tsyringe/src/index.test.ts b/packages/tsyringe/src/index.test.ts
new file mode 100644
index 00000000..43f7e828
--- /dev/null
+++ b/packages/tsyringe/src/index.test.ts
@@ -0,0 +1,57 @@
+import 'reflect-metadata'
+import { injectable, inject } from 'tsyringe'
+import { Hono } from 'hono'
+import { tsyringe } from '../src'
+
+class Config {
+  constructor(public readonly tenantName: string) {}
+}
+
+@injectable()
+class TenantService {
+  constructor(@inject(Config) public readonly config: Config) {}
+
+  get message() {
+    return `Hello, ${this.config.tenantName}!`
+  }
+}
+
+describe('tsyringe middleware', () => {
+  const app = new Hono()
+
+  app.use(
+    '/hello/*',
+    tsyringe((container) => container.register('foo', { useValue: 'Hello!' }))
+  )
+  app.get('/hello/foo', (c) => {
+    const message = c.var.resolve<string>('foo')
+    return c.text(message)
+  })
+
+  app.use('/tenant/:name/*', async (c, next) => {
+    await tsyringe((container) => {
+      const tenantName = c.req.param('name')
+
+      container.register(Config, { useFactory: () => new Config(tenantName) })
+    })(c, next)
+  })
+
+  app.get('/tenant/:name/message', (c) => {
+    const tenantService = c.var.resolve(TenantService)
+    return c.text(tenantService.message)
+  })
+
+  it('Should be hello message', async () => {
+    const res = await app.request('http://localhost/hello/foo')
+    expect(res).not.toBeNull()
+    expect(res.status).toBe(200)
+    expect(await res.text()).toBe('Hello!')
+  })
+
+  it('Should be tenant message', async () => {
+    const res = await app.request('http://localhost/tenant/foo/message')
+    expect(res).not.toBeNull()
+    expect(res.status).toBe(200)
+    expect(await res.text()).toBe('Hello, foo!')
+  })
+})
diff --git a/packages/tsyringe/src/index.ts b/packages/tsyringe/src/index.ts
new file mode 100644
index 00000000..1a0c25ec
--- /dev/null
+++ b/packages/tsyringe/src/index.ts
@@ -0,0 +1,19 @@
+import { container, DependencyContainer, InjectionToken } from 'tsyringe'
+import type { Context, MiddlewareHandler } from 'hono'
+import { createMiddleware } from 'hono/factory'
+
+declare module 'hono' {
+  interface ContextVariableMap {
+    resolve: <T>(token: InjectionToken<T>) => T
+  }
+}
+
+export type Provider = (container: DependencyContainer) => void
+export const tsyringe = (...providers: Provider[]): MiddlewareHandler => {
+  return createMiddleware(async (c, next) => {
+    const childContainer = container.createChildContainer()
+    providers.forEach((provider) => provider(childContainer))
+    c.set('resolve', <T>(token: InjectionToken<T>) => childContainer.resolve(token))
+    await next()
+  })
+}
diff --git a/packages/tsyringe/tsconfig.json b/packages/tsyringe/tsconfig.json
new file mode 100644
index 00000000..3cbc5a07
--- /dev/null
+++ b/packages/tsyringe/tsconfig.json
@@ -0,0 +1,12 @@
+{
+  "extends": "../../tsconfig.json",
+  "compilerOptions": {
+    "rootDir": "./src",
+    "outDir": "./dist",
+    "experimentalDecorators": true,
+    "emitDecoratorMetadata": true
+  },
+  "include": [
+    "src/**/*.ts"
+  ],
+}
diff --git a/packages/tsyringe/vitest.config.ts b/packages/tsyringe/vitest.config.ts
new file mode 100644
index 00000000..17b54e48
--- /dev/null
+++ b/packages/tsyringe/vitest.config.ts
@@ -0,0 +1,8 @@
+/// <reference types="vitest" />
+import { defineConfig } from 'vitest/config'
+
+export default defineConfig({
+  test: {
+    globals: true,
+  },
+})
diff --git a/yarn.lock b/yarn.lock
index 8b66811c..3c6a33b2 100644
--- a/yarn.lock
+++ b/yarn.lock
@@ -2863,6 +2863,22 @@ __metadata:
   languageName: unknown
   linkType: soft
 
+"@hono/tsyringe@workspace:packages/tsyringe":
+  version: 0.0.0-use.local
+  resolution: "@hono/tsyringe@workspace:packages/tsyringe"
+  dependencies:
+    hono: "npm:^4.4.12"
+    prettier: "npm:^3.3.3"
+    reflect-metadata: "npm:^0.2.2"
+    tsup: "npm:^8.1.0"
+    tsyringe: "npm:^4.8.0"
+    vitest: "npm:^1.6.0"
+  peerDependencies:
+    hono: ">=4.*"
+    tsyringe: ">=4.*"
+  languageName: unknown
+  linkType: soft
+
 "@hono/typebox-validator@workspace:packages/typebox-validator":
   version: 0.0.0-use.local
   resolution: "@hono/typebox-validator@workspace:packages/typebox-validator"
@@ -16823,6 +16839,15 @@ __metadata:
   languageName: node
   linkType: hard
 
+"prettier@npm:^3.3.3":
+  version: 3.3.3
+  resolution: "prettier@npm:3.3.3"
+  bin:
+    prettier: bin/prettier.cjs
+  checksum: b85828b08e7505716324e4245549b9205c0cacb25342a030ba8885aba2039a115dbcf75a0b7ca3b37bc9d101ee61fab8113fc69ca3359f2a226f1ecc07ad2e26
+  languageName: node
+  linkType: hard
+
 "pretty-format@npm:^28.0.0, pretty-format@npm:^28.1.3":
   version: 28.1.3
   resolution: "pretty-format@npm:28.1.3"
@@ -19522,7 +19547,7 @@ __metadata:
   languageName: node
   linkType: hard
 
-"tslib@npm:^1.8.1, tslib@npm:^1.9.0":
+"tslib@npm:^1.8.1, tslib@npm:^1.9.0, tslib@npm:^1.9.3":
   version: 1.14.1
   resolution: "tslib@npm:1.14.1"
   checksum: 69ae09c49eea644bc5ebe1bca4fa4cc2c82b7b3e02f43b84bd891504edf66dbc6b2ec0eef31a957042de2269139e4acff911e6d186a258fb14069cd7f6febce2
@@ -19830,6 +19855,15 @@ __metadata:
   languageName: node
   linkType: hard
 
+"tsyringe@npm:^4.8.0":
+  version: 4.8.0
+  resolution: "tsyringe@npm:4.8.0"
+  dependencies:
+    tslib: "npm:^1.9.3"
+  checksum: e13810e8ff39c4093acd0649bc5db3c164825827631e1522cd9d5ca8694a018447fa1c24f059ea54e93b1020767b1131b9dc9ce598dabfc9aa41c11544bbfe19
+  languageName: node
+  linkType: hard
+
 "tty-table@npm:^4.1.5":
   version: 4.2.3
   resolution: "tty-table@npm:4.2.3"