diff --git a/tests/unit/test_io_hdf5_h5tools.py b/tests/unit/test_io_hdf5_h5tools.py
index 7f894c900..58119ce9b 100644
--- a/tests/unit/test_io_hdf5_h5tools.py
+++ b/tests/unit/test_io_hdf5_h5tools.py
@@ -3861,8 +3861,30 @@ class MyData(Data):
             pass
 
         self.data = MyData("my_data", [1, 2, 3])
+        self.file_path = get_temp_filepath()
+
+    def tearDown(self):
+        if os.path.exists(self.file_path):
+            os.remove(self.file_path)
 
     def test_set_data_io(self):
         self.data.set_data_io(H5DataIO, dict(chunks=True))
         assert isinstance(self.data.data, H5DataIO)
         assert self.data.data.io_settings["chunks"]
+
+    def test_set_data_io_h5py_dataset(self):
+        file = File(self.file_path, 'w')
+        data = file.create_dataset('data', data=[1, 2, 3, 4, 5], chunks=(3,))
+        class MyData(Data):
+            pass
+
+        my_data = MyData("my_data", data)
+        my_data.set_data_io(
+            H5DataIO,
+            data_io_kwargs=dict(chunks=(2,)),
+            data_chunk_iterator_class=DataChunkIterator,
+        )
+
+        self.assertIsInstance(my_data.data, H5DataIO)
+        self.assertEqual(my_data.data.io_settings["chunks"], (2,))
+        file.close()