diff --git a/.changelog/22664.txt b/.changelog/22664.txt
new file mode 100644
index 00000000000..5a7cde63858
--- /dev/null
+++ b/.changelog/22664.txt
@@ -0,0 +1,7 @@
+```release-note:note
+resource/aws_route: The `instance_id` argument has been deprecated. All configurations using `instance_id` should be updated to use the `network_interface_id` argument instead
+```
+
+```release-note:note
+resource/aws_route_table: The `instance_id` argument of the `route` configuration block has been deprecated. All configurations using `route` `instance_id` should be updated to use the `route` `network_interface_id` argument instead
+```
\ No newline at end of file
diff --git a/internal/service/ec2/route.go b/internal/service/ec2/route.go
index 91cc643f8f7..445f5b5c720 100644
--- a/internal/service/ec2/route.go
+++ b/internal/service/ec2/route.go
@@ -110,6 +110,7 @@ func ResourceRoute() *schema.Resource {
 				Type:         schema.TypeString,
 				Optional:     true,
 				Computed:     true,
+				Deprecated:   "Use network_interface_id instead",
 				ExactlyOneOf: routeValidTargets,
 			},
 			"local_gateway_id": {
diff --git a/internal/service/ec2/route_table.go b/internal/service/ec2/route_table.go
index fd40abb9f7c..d479c1a5b72 100644
--- a/internal/service/ec2/route_table.go
+++ b/internal/service/ec2/route_table.go
@@ -121,8 +121,9 @@ func ResourceRouteTable() *schema.Resource {
 							Optional: true,
 						},
 						"instance_id": {
-							Type:     schema.TypeString,
-							Optional: true,
+							Type:       schema.TypeString,
+							Optional:   true,
+							Deprecated: "Use network_interface_id instead",
 						},
 						"local_gateway_id": {
 							Type:     schema.TypeString,
diff --git a/website/docs/r/route.html.markdown b/website/docs/r/route.html.markdown
index 42ca414ded4..12702130c5a 100644
--- a/website/docs/r/route.html.markdown
+++ b/website/docs/r/route.html.markdown
@@ -61,7 +61,7 @@ One of the following target arguments must be supplied:
 * `carrier_gateway_id` - (Optional) Identifier of a carrier gateway. This attribute can only be used when the VPC contains a subnet which is associated with a Wavelength Zone.
 * `egress_only_gateway_id` - (Optional) Identifier of a VPC Egress Only Internet Gateway.
 * `gateway_id` - (Optional) Identifier of a VPC internet gateway or a virtual private gateway.
-* `instance_id` - (Optional) Identifier of an EC2 instance.
+* `instance_id` - (Optional, **Deprecated** use `network_interface_id` instead) Identifier of an EC2 instance.
 * `nat_gateway_id` - (Optional) Identifier of a VPC NAT gateway.
 * `local_gateway_id` - (Optional) Identifier of a Outpost local gateway.
 * `network_interface_id` - (Optional) Identifier of an EC2 network interface.
diff --git a/website/docs/r/route_table.html.markdown b/website/docs/r/route_table.html.markdown
index 37779a3df5a..2ad02c9968e 100644
--- a/website/docs/r/route_table.html.markdown
+++ b/website/docs/r/route_table.html.markdown
@@ -90,7 +90,7 @@ One of the following target arguments must be supplied:
 * `carrier_gateway_id` - (Optional) Identifier of a carrier gateway. This attribute can only be used when the VPC contains a subnet which is associated with a Wavelength Zone.
 * `egress_only_gateway_id` - (Optional) Identifier of a VPC Egress Only Internet Gateway.
 * `gateway_id` - (Optional) Identifier of a VPC internet gateway or a virtual private gateway.
-* `instance_id` - (Optional) Identifier of an EC2 instance.
+* `instance_id` - (Optional, **Deprecated** use `network_interface_id` instead) Identifier of an EC2 instance.
 * `local_gateway_id` - (Optional) Identifier of a Outpost local gateway.
 * `nat_gateway_id` - (Optional) Identifier of a VPC NAT gateway.
 * `network_interface_id` - (Optional) Identifier of an EC2 network interface.