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README.md

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This repository contains scripts/pipelines for Jiang et al 2021. eLife: A modified fluctuation assay reveals a natural mutator phenotype that drives mutation spectrum variation within Saccharomyces cerevisiae

fig 1: analyzing mutation spectra from polymorphism data: preprocess of aligning S.paradoxus to S.cerevisiae: Spar_preprocess/, scripts for generating plots are in script/

  • need to align outgroup (S.para) to S.cer first. see directory: Spar_preprocess/

  • script to plot fig1 and related supplementary figures script/plot_fig1_and_suppl.R script/plot_fig1_PCA_Scer_AC4.R

fig 3: fluctuation assay: fluc/

plot_mut_rate_fig3.R

fig 4: mutation spectra from de novo mutations: CAN1_mut_sequencing/

check the readme file in that directory. first call mutations from raw illumina sequencing reads, identify MNMs, and get the final set of single nucleotide mutations

  • script to plot fig 4 CAN1_mut_sequencing/script/fig4_from_10_21_final.R

fig 5: C to A ratio enrichment: dipCBS1782/

refer to the README file in that directory.

fig 6: mutation rate estimates from strains transformed with different OGG1 plasmids: OGG1/

figure 6: OGG1/plot_mut_rate.R