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I ran the python command and for some reason from the branches and CNV_Files there is no information for some sub clones. For example the unique sub clone names from cell groupings was
[1] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.1.1.1"
[2] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.1.1.2"
[3] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.1.2.1"
[4] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.1.2.2"
[5] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.2.1.1"
[6] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.2.1.2"
[7] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.2.2.1"
[8] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.2.2.2"
but in the CNV_files there is only
1,0.0
1.1,0.0,B
1.1.1,50.25716385011021,C
1.1.2,5.6208670095518,D
1.2,0.0,E
1.2.1,9.184423218221896,F
1.2.2,34.937545922116094,G
There never seems to cluster names with all four digits.
The text was updated successfully, but these errors were encountered:
jfwise
changed the title
Missing branches beginning with 2
Missing sub clones
Jul 15, 2022
Hi! I know it is too late and probably you have already sorted it out, but writing it for others from my experience (and also to know if I misunderstood anything). I think the missing subclones are due to the threshold set in the uphyloplot2 python script. For example, I got less subclones in the uphyloplot2 output when I used "python uphyloplot2.py -c 10", even less by "python uphyloplot2.py -c 15" and so on. The default is set to 5 (Only subclones that comprise at least 5% of cells will be included for plotting), so it will filter out some subclones (as we see in our input .cell_groupings file) even if we do not set a threshold ourselves.
I ran the python command and for some reason from the branches and CNV_Files there is no information for some sub clones. For example the unique sub clone names from cell groupings was
[1] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.1.1.1"
[2] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.1.1.2"
[3] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.1.2.1"
[4] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.1.2.2"
[5] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.2.1.1"
[6] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.2.1.2"
[7] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.2.2.1"
[8] "malignant_HPT1Pat1.malignant_HPT1Pat1.1.2.2.2"
but in the CNV_files there is only
1,0.0
1.1,0.0,B
1.1.1,50.25716385011021,C
1.1.2,5.6208670095518,D
1.2,0.0,E
1.2.1,9.184423218221896,F
1.2.2,34.937545922116094,G
There never seems to cluster names with all four digits.
The text was updated successfully, but these errors were encountered: