From 702c57d8d956ab33336b591b22bf9f8b0c41a3e0 Mon Sep 17 00:00:00 2001 From: Ronan Lordan <62627112+RLordan@users.noreply.github.com> Date: Thu, 6 Jan 2022 13:09:34 -0500 Subject: [PATCH] Update content/10.diagnostics.md Co-authored-by: HM Rando --- content/10.diagnostics.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/content/10.diagnostics.md b/content/10.diagnostics.md index d7df25c1c..205a5460b 100644 --- a/content/10.diagnostics.md +++ b/content/10.diagnostics.md @@ -133,7 +133,7 @@ One study showed that RT-LAMP is effective for detection of SARS-CoV-2 with exce This test aims to bring the sensitivity of nucleic acid detection to the point of care or home testing setting. It could be applied for screening, diagnostics, or as a definitive test for people who are positive based on lateral flow tests. This method was benchmarked against RT-PCR using 177 human nasopharyngeal RNA samples, of which 126 were COVID positive. -The authors break down the sensitivity of their test according to the cycle threshold (Ct) value from RT-PCR of the same samples; RT-LAMP performs at 100% sensitivity for samples with a Ct from RT-PCR of 32 or less. +The authors break down the sensitivity of their test according to the cycle threshold (C~t~) value from RT-PCR of the same samples; RT-LAMP performs at 100% sensitivity for samples with a C~t~ from RT-PCR of 32 or less. The performance is worse when considering all RT-PCR positive samples (including those with C~t~ values between 32-40). However, there is some evidence suggesting that samples obtained from individuals that achieve Ct values >30 measured using RT-PCR tend to be less infective that those that record a Ct value <30 [@doi:10.1016/j.jiph.2021.08.013; @doi:10.1017/ice.2021.132; @doi:10.1093/cid/ciaa619], and so RT-LAMP is still a useful diagnostic tool. Various combinations of reagents are available, but one example of reagents being used are the WarmStart Colorimetric LAMP 2X Master Mix with a set of six primers developed previously by Zhang et al. [@doi:10.1101/2020.02.26.20028373].