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This repository has been archived by the owner on Jul 20, 2021. It is now read-only.
At the moment the points you manually specify are considered fixed, so won't be adjusted by the "Fit Volume" option, for example. This is a limitation that I intend to fix, but it needs a bit of careful work. There are two plans I have for this:
Allow manual adjustment of where the points are at any stage
Allowing the fitting to adjust the manually placed points as well as the intermediate points
In the mean time, my suggestion would be to select the intermediate points in a zoomed-in view, using the three-pane view to find the midline of the neuron. There's a short tutorial here explaining what I mean:
This could be tackled in multiple ways. v3.1.0 implements a snap-to function in which the cursor will automatically 'sniff' for local maxima and 'snap' to their average X,Y,Z position. This functionallity was inspired by NeuronJ. Here is a description from its manual (accessed 2016.12.30):
To facilitate accurate positioning of starting points, so that they are really on a neurite rather than close to one, the program carries out a local snapping operation. This means that when moving the mouse within the image, the program quickly searches in a small window around the current mouse position for the pixel that is most likely to be on a neurite. This position is indicated by the red cross-hair cursor which is behind the real, white cross-hair cursor.
But in SNT, this functionality works in 3D (there is an option in the GUI to restrict it to 2D if needed).
So, I consider this issue now closed. It could be reopened if someone feels otherwise.
From @mhl:
Migrated-From: http://fiji.sc/bugzilla/show_bug.cgi?id=222
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