diff --git a/django/test/testcases.py b/django/test/testcases.py
index 51b07ae50d4e..bf035bd531fc 100644
--- a/django/test/testcases.py
+++ b/django/test/testcases.py
@@ -280,6 +280,8 @@ def patched_ensure_connection(self, *args, **kwargs):
                 self.connection is None
                 and self.alias not in cls.databases
                 and self.alias != NO_DB_ALIAS
+                # Dynamically created connections are always allowed.
+                and self.alias in connections
             ):
                 # Connection has not yet been established, but the alias is not allowed.
                 message = cls._disallowed_database_msg % {
diff --git a/tests/test_utils/tests.py b/tests/test_utils/tests.py
index 7e9505f76210..fab7f27aa11f 100644
--- a/tests/test_utils/tests.py
+++ b/tests/test_utils/tests.py
@@ -2161,6 +2161,16 @@ def thread_func():
                     conn.close()
                     conn.dec_thread_sharing()
 
+    def test_allowed_database_copy_queries(self):
+        new_connection = connection.copy("dynamic_connection")
+        try:
+            with new_connection.cursor() as cursor:
+                sql = f"SELECT 1{new_connection.features.bare_select_suffix}"
+                cursor.execute(sql)
+                self.assertEqual(cursor.fetchone()[0], 1)
+        finally:
+            new_connection.close()
+
 
 class DatabaseAliasTests(SimpleTestCase):
     def setUp(self):