ImportError: Cannot load backend 'TkAgg' which requires the 'tk' interactive framework, as 'headless' is currently running

[dm8000]

Hello

I have been trying to reproduce this. However, I keep getting an error with TkAgg.

This is the block that is giving me an error (with a few modifications, but the original gives me the same message)

import os              
os.environ['PYTHONHASHSEED'] = '0'

import matplotlib
matplotlib.use('TKAgg', force = True)
import matplotlib.pyplot as plt

import desc          
import pandas as pd                                                    
import numpy as np                                                     
import scanpy.api as sc                                                                                 
from time import time                                                       
import sys
import matplotlib.pyplot as plt
%matplotlib inline 
sc.settings.set_figure_params(dpi=300)

The output is:

---------------------------------------------------------------------------
ImportError                               Traceback (most recent call last)
<ipython-input-1-9cdf2f4155a6> in <module>
      4 import matplotlib
      5 matplotlib.use('TKAgg', force = True)
----> 6 import matplotlib.pyplot as plt
      7 
      8 import desc

/data04/projects04/MarianaBoroni/lbbc_members/lib/conda_envs/diogoamb/lib/python3.9/site-packages/matplotlib/pyplot.py in <module>
   2228     dict.__setitem__(rcParams, "backend", rcsetup._auto_backend_sentinel)
   2229 # Set up the backend.
-> 2230 switch_backend(rcParams["backend"])
   2231 
   2232 # Just to be safe.  Interactive mode can be turned on without

/data04/projects04/MarianaBoroni/lbbc_members/lib/conda_envs/diogoamb/lib/python3.9/site-packages/matplotlib/pyplot.py in switch_backend(newbackend)
    273         if (current_framework and required_framework
    274                 and current_framework != required_framework):
--> 275             raise ImportError(
    276                 "Cannot load backend {!r} which requires the {!r} interactive "
    277                 "framework, as {!r} is currently running".format(

ImportError: Cannot load backend 'TkAgg' which requires the 'tk' interactive framework, as 'headless' is currently running

It seemed to be a problem with TkAgg, (hence why I modified the first few lines). But when I run

print("Using:", matplotlib.get_backend())

It seems the backend TkAgg can be used fine:

Using: TkAgg

Here is the output when I run exactly what is in the ttutorial

import os              
os.environ['PYTHONHASHSEED'] = '0'
import desc          
import pandas as pd                                                    
import numpy as np                                                     
import scanpy.api as sc                                                                                 
from time import time                                                       
import sys
import matplotlib
import matplotlib.pyplot as plt
%matplotlib inline 
sc.settings.set_figure_params(dpi=300)

`---------------------------------------------------------------------------
ImportError                               Traceback (most recent call last)
<ipython-input-2-792688ab25c3> in <module>
      1 import os
      2 os.environ['PYTHONHASHSEED'] = '0'
----> 3 import desc
      4 import pandas as pd
      5 import numpy as np

/data04/projects04/MarianaBoroni/lbbc_members/lib/conda_envs/diogoamb/lib/python3.9/site-packages/desc/__init__.py in <module>
      1 #from . import original as og
----> 2 from . import tools
      3 from . import models
      4 from . import datasets
      5 

/data04/projects04/MarianaBoroni/lbbc_members/lib/conda_envs/diogoamb/lib/python3.9/site-packages/desc/tools/__init__.py in <module>
      2 from scanpy.preprocessing import normalize_per_cell, highly_variable_genes, log1p, scale
      3 
----> 4 from .test import run_desc_test
      5 from .read import read_10X
      6 from .write import write_desc_result

/data04/projects04/MarianaBoroni/lbbc_members/lib/conda_envs/diogoamb/lib/python3.9/site-packages/desc/tools/test.py in <module>
      1 
      2 from ..datasets import pbmc_processed
----> 3 from ..models.desc import train
      4 
      5 

/data04/projects04/MarianaBoroni/lbbc_members/lib/conda_envs/diogoamb/lib/python3.9/site-packages/desc/models/__init__.py in <module>
----> 1 from .desc import train
      2 

/data04/projects04/MarianaBoroni/lbbc_members/lib/conda_envs/diogoamb/lib/python3.9/site-packages/desc/models/desc.py in <module>
     22 #if we have a display use a plotting backend
     23 if havedisplay:
---> 24     matplotlib.use('TkAgg')
     25 else:
     26     matplotlib.use('Agg')

/data04/projects04/MarianaBoroni/lbbc_members/lib/conda_envs/diogoamb/lib/python3.9/site-packages/matplotlib/__init__.py in use(backend, force)
   1142                 # user does not have the libraries to support their
   1143                 # chosen backend installed.
-> 1144                 plt.switch_backend(name)
   1145             except ImportError:
   1146                 if force:

/data04/projects04/MarianaBoroni/lbbc_members/lib/conda_envs/diogoamb/lib/python3.9/site-packages/matplotlib/pyplot.py in switch_backend(newbackend)
    273         if (current_framework and required_framework
    274                 and current_framework != required_framework):
--> 275             raise ImportError(
    276                 "Cannot load backend {!r} which requires the {!r} interactive "
    277                 "framework, as {!r} is currently running".format(

ImportError: Cannot load backend 'TkAgg' which requires the 'tk' interactive framework, as 'headless' is currently running`

Any ideas?