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nextflow.config
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process {
queue="$SCXA_LSF_QUEUE"
clusterOptions="$SCXA_LSF_OPTIONS"
executor='lsf'
withName: 'download_fastqs' {
clusterOptions = "-g /scxa_ena_download"
}
}
executor {
queueSize=500
perJobMemLimit=true
exitReadTimeout='100000 sec'
pollInterval = '5sec'
}
conda {
cacheDir = "$SCXA_WORKFLOW_ROOT/envs"
createTimeout = "30 min"
}
params {
downloadMethod = 'http'
maxConcurrentDownloads = 10
minMappingRate = 12
minCbFreq = 10
'emptyDrops' {
lower = 100
nIters = 1000
testAmbient = 'FALSE'
filterEmpty = 'TRUE'
filterFdr = '0.01'
libType = 'ISR'
}
'10xv2' {
alevinType = 'chromium'
barcodeLength = 16
umiLength = 10
end = '5'
libType = 'ISR'
}
'drop-seq' {
alevinType = 'dropseq'
barcodeLength = 12
umiLength = 8
end = '5'
libType = 'ISR'
}
'seq-well' {
alevinType = 'dropseq'
barcodeLength = 12
umiLength = 8
end = '5'
libType = 'ISR'
}
'10xv3' {
alevinType = 'chromiumV3'
barcodeLength = 16
umiLength = 12
end = '5'
libType = 'ISR'
}
'10x5prime' {
alevinType = 'custom'
barcodeLength = 16
umiLength = 10
end = '5'
libType = 'ISF'
}
salmon {
index {
kmerSize = 31
}
}
}