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nextflow.config
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params {
fastq = "*_{1,2}.fastq.gz"
assemblies = false
assembly_loc = "assemblies/*.fasta"
species = "Burkholderia_pseudomallei"
patientMetaData = "${baseDir}/Reports/data/patientMetaData.csv"
gwas = false
notrim = true
matrix = true
annotate = true
mixtures = false
phylogeny = false
antibiotic_res = true
strain = "all"
tech = "Illumina"
pairing = "PE"
window = 10000
indel_merge = true
tri_tetra_allelic = false
size = 1000000
phred = "-phred33"
org = "haploid"
executor = "local"
delly = true
fast = true
}
includeConfig 'configs/gatk.config'
conda.enabled = true
process {
if(params.executor != "local") {
conda = "$CONDA_PREFIX"
}
executor = params.executor
errorStrategy = "retry"
maxRetries = 4
withLabel: art {
cpus = 1
memory = { 4.GB * task.attempt }
time = "8h"
}
withLabel: card {
cpus = 1
memory = "4G"
time = "8h"
}
withLabel: index {
cpus = 1
memory = "4G"
time = "8h"
}
withLabel: ardap_default {
cpus = 2
memory = { 4.GB * task.attempt }
time = "24h"
}
withLabel: alignment {
cpus = 4
memory = { 4.GB * task.attempt }
time = "24h"
}
withLabel: trimmomatic {
cpus = 2
memory = { 4.GB * task.attempt }
time = "24h"
}
withLabel: markduplicates {
cpus = 2
memory = { 4.GB * task.attempt }
time = "24h"
}
withLabel: gatk_haplo {
cpus = 4
memory = { 4.GB * task.attempt }
time = "24h"
}
withLabel: gatk {
cpus = 4
memory = { 4.GB * task.attempt }
time = "24h"
}
withLabel: snpeff {
cpus = 1
memory = { 4.GB * task.attempt }
time = "24h"
}
withLabel: pindel {
cpus = 2
memory = { 4.GB * task.attempt }
time = "96h"
}
withLabel: genomic_queries {
cpus = 1
memory = "4G"
time = "8h"
}
withLabel: card_queries {
cpus = 1
memory = "4G"
time = "2h"
}
withLabel: report {
cpus = 1
memory = "4G"
time = "2h"
}
withLabel: master_vcf {
cpus = 1
memory = { 4.GB * task.attempt }
time = "24h"
}
withLabel: snp_matrix {
cpus = 1
memory = { 4.GB * task.attempt }
time = "24h"
}
}
manifest {
homePage = 'http://github.com/dsarov/ARDaP'
description = 'Comprehensive resistance detection from WGS'
mainScript = 'main.nf'
version = '1.9'
}