diff --git a/src/libraries/tests.proj b/src/libraries/tests.proj
index 47fc849ed6ede..0b131e6efee9c 100644
--- a/src/libraries/tests.proj
+++ b/src/libraries/tests.proj
@@ -323,8 +323,6 @@
     <!-- App Crash https://github.com/dotnet/runtime/issues/53624 -->
     <ProjectExclusions Include="$(MSBuildThisFileDirectory)System.Net.Requests/tests/System.Net.Requests.Tests.csproj" />
     <ProjectExclusions Include="$(MSBuildThisFileDirectory)System.Net.Security/tests/FunctionalTests/System.Net.Security.Tests.csproj" />
-    <!-- Reference deleted by _CopyTestArchive https://github.com/dotnet/runtime/issues/80976 -->
-    <ProjectExclusions Include="$(RepoRoot)/src/tests/FunctionalTests/iOS/Simulator/StartupHook/iOS.Simulator.StartupHook.Test.csproj" />
   </ItemGroup>
 
   <ItemGroup Condition="'$(TargetOS)' == 'browser' and '$(RunDisabledWasmTests)' != 'true' and '$(RunAOTCompilation)' != 'true'">