diff --git a/haystack/components/preprocessors/document_splitter.py b/haystack/components/preprocessors/document_splitter.py
index 4eb15aeec2..556878a965 100644
--- a/haystack/components/preprocessors/document_splitter.py
+++ b/haystack/components/preprocessors/document_splitter.py
@@ -3,11 +3,13 @@
 # SPDX-License-Identifier: Apache-2.0
 
 from copy import deepcopy
-from typing import Callable, Dict, List, Literal, Optional, Tuple
+from typing import Any, Callable, Dict, List, Literal, Optional, Tuple
 
 from more_itertools import windowed
 
 from haystack import Document, component
+from haystack.core.serialization import default_from_dict, default_to_dict
+from haystack.utils import deserialize_callable, serialize_callable
 
 
 @component
@@ -243,3 +245,31 @@ def _add_split_overlap_information(
                 # add split overlap information to previous Document regarding this Document
                 overlapping_range = (0, overlapping_range[1] - overlapping_range[0])
                 previous_doc.meta["_split_overlap"].append({"doc_id": current_doc.id, "range": overlapping_range})
+
+    def to_dict(self) -> Dict[str, Any]:
+        """
+        Serializes the component to a dictionary.
+        """
+        serialized = default_to_dict(
+            self,
+            split_by=self.split_by,
+            split_length=self.split_length,
+            split_overlap=self.split_overlap,
+            split_threshold=self.split_threshold,
+        )
+        if self.splitting_function:
+            serialized["init_parameters"]["splitting_function"] = serialize_callable(self.splitting_function)
+        return serialized
+
+    @classmethod
+    def from_dict(cls, data: Dict[str, Any]) -> "DocumentSplitter":
+        """
+        Deserializes the component from a dictionary.
+        """
+        init_params = data.get("init_parameters", {})
+
+        splitting_function = init_params.get("splitting_function", None)
+        if splitting_function:
+            init_params["splitting_function"] = deserialize_callable(splitting_function)
+
+        return default_from_dict(cls, data)
diff --git a/test/components/preprocessors/test_document_splitter.py b/test/components/preprocessors/test_document_splitter.py
index edad6ddb72..7c942ab4cc 100644
--- a/test/components/preprocessors/test_document_splitter.py
+++ b/test/components/preprocessors/test_document_splitter.py
@@ -7,6 +7,12 @@
 
 from haystack import Document
 from haystack.components.preprocessors import DocumentSplitter
+from haystack.utils import deserialize_callable, serialize_callable
+
+
+# custom split function for testing
+def custom_split(text):
+    return text.split(".")
 
 
 def merge_documents(documents):
@@ -352,3 +358,90 @@ def test_add_split_overlap_information(self):
 
         # reconstruct the original document content from the split documents
         assert doc.content == merge_documents(docs)
+
+    def test_to_dict(self):
+        """
+        Test the to_dict method of the DocumentSplitter class.
+        """
+        splitter = DocumentSplitter(split_by="word", split_length=10, split_overlap=2, split_threshold=5)
+        serialized = splitter.to_dict()
+
+        assert serialized["type"] == "haystack.components.preprocessors.document_splitter.DocumentSplitter"
+        assert serialized["init_parameters"]["split_by"] == "word"
+        assert serialized["init_parameters"]["split_length"] == 10
+        assert serialized["init_parameters"]["split_overlap"] == 2
+        assert serialized["init_parameters"]["split_threshold"] == 5
+        assert "splitting_function" not in serialized["init_parameters"]
+
+    def test_to_dict_with_splitting_function(self):
+        """
+        Test the to_dict method of the DocumentSplitter class when a custom splitting function is provided.
+        """
+
+        splitter = DocumentSplitter(split_by="function", splitting_function=custom_split)
+        serialized = splitter.to_dict()
+
+        assert serialized["type"] == "haystack.components.preprocessors.document_splitter.DocumentSplitter"
+        assert serialized["init_parameters"]["split_by"] == "function"
+        assert "splitting_function" in serialized["init_parameters"]
+        assert callable(deserialize_callable(serialized["init_parameters"]["splitting_function"]))
+
+    def test_from_dict(self):
+        """
+        Test the from_dict class method of the DocumentSplitter class.
+        """
+        data = {
+            "type": "haystack.components.preprocessors.document_splitter.DocumentSplitter",
+            "init_parameters": {"split_by": "word", "split_length": 10, "split_overlap": 2, "split_threshold": 5},
+        }
+        splitter = DocumentSplitter.from_dict(data)
+
+        assert splitter.split_by == "word"
+        assert splitter.split_length == 10
+        assert splitter.split_overlap == 2
+        assert splitter.split_threshold == 5
+        assert splitter.splitting_function is None
+
+    def test_from_dict_with_splitting_function(self):
+        """
+        Test the from_dict class method of the DocumentSplitter class when a custom splitting function is provided.
+        """
+
+        def custom_split(text):
+            return text.split(".")
+
+        data = {
+            "type": "haystack.components.preprocessors.document_splitter.DocumentSplitter",
+            "init_parameters": {"split_by": "function", "splitting_function": serialize_callable(custom_split)},
+        }
+        splitter = DocumentSplitter.from_dict(data)
+
+        assert splitter.split_by == "function"
+        assert callable(splitter.splitting_function)
+        assert splitter.splitting_function("a.b.c") == ["a", "b", "c"]
+
+    def test_roundtrip_serialization(self):
+        """
+        Test the round-trip serialization of the DocumentSplitter class.
+        """
+        original_splitter = DocumentSplitter(split_by="word", split_length=10, split_overlap=2, split_threshold=5)
+        serialized = original_splitter.to_dict()
+        deserialized_splitter = DocumentSplitter.from_dict(serialized)
+
+        assert original_splitter.split_by == deserialized_splitter.split_by
+        assert original_splitter.split_length == deserialized_splitter.split_length
+        assert original_splitter.split_overlap == deserialized_splitter.split_overlap
+        assert original_splitter.split_threshold == deserialized_splitter.split_threshold
+
+    def test_roundtrip_serialization_with_splitting_function(self):
+        """
+        Test the round-trip serialization of the DocumentSplitter class when a custom splitting function is provided.
+        """
+
+        original_splitter = DocumentSplitter(split_by="function", splitting_function=custom_split)
+        serialized = original_splitter.to_dict()
+        deserialized_splitter = DocumentSplitter.from_dict(serialized)
+
+        assert original_splitter.split_by == deserialized_splitter.split_by
+        assert callable(deserialized_splitter.splitting_function)
+        assert deserialized_splitter.splitting_function("a.b.c") == ["a", "b", "c"]