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Bug when changing size of volcano plot points #169

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denklewer opened this issue Sep 14, 2024 · 0 comments
Open

Bug when changing size of volcano plot points #169

denklewer opened this issue Sep 14, 2024 · 0 comments

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@denklewer
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denklewer commented Sep 14, 2024

When I am changing size of the points in volcano plot they seem to be plotted with wrong coordinates if p-value is > 0

Steps to repropduce:

  1. Open dataset, perform lima
  2. Select several genes. add annotation which will represent points size (Edit/Annotate Selected rows). I used value 1
    image
  3. Invert selection and annotate other genes with different size. (I used 0.1)
  4. Open original volcano plot (Tools/Plots/Volcano)
    image
  5. Specify size of the points to be read from the IFN field
    image
  6. Result plot will be looking like this
    image
  7. However after setting P value significance to 0 the positions of colored points are changed
    image
  8. Positions with value 0 seems to be more accurate according to label by selected check mark
    image
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