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When parsing fastq file, convert to BAM #14

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cmatKhan opened this issue Jun 19, 2024 · 0 comments
Open

When parsing fastq file, convert to BAM #14

cmatKhan opened this issue Jun 19, 2024 · 0 comments
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enhancement New feature or request

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@cmatKhan
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Description of feature

It looks like in fastquorum from fgbio, they parse fastq with umi into a bam with tags. In the alignment step, they use samtools fastq to turn the bam back into a fastq file for alignment with bwa mem.

It is worth looking at this -- if the tags added to the fastq file are retained when converting from bam back to fastq back to bam, then it could potentially make the processing more straightforward

https://github.com/nf-core/fastquorum/blob/ea307015cf134358883d48fcd7a86b365a0a8bb2/modules/local/align_bam/main.nf

@cmatKhan cmatKhan added the enhancement New feature or request label Jun 19, 2024
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