You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
It looks like in fastquorum from fgbio, they parse fastq with umi into a bam with tags. In the alignment step, they use samtools fastq to turn the bam back into a fastq file for alignment with bwa mem.
It is worth looking at this -- if the tags added to the fastq file are retained when converting from bam back to fastq back to bam, then it could potentially make the processing more straightforward
Description of feature
It looks like in fastquorum from fgbio, they parse fastq with umi into a bam with tags. In the alignment step, they use samtools fastq to turn the bam back into a fastq file for alignment with bwa mem.
It is worth looking at this -- if the tags added to the fastq file are retained when converting from bam back to fastq back to bam, then it could potentially make the processing more straightforward
https://github.com/nf-core/fastquorum/blob/ea307015cf134358883d48fcd7a86b365a0a8bb2/modules/local/align_bam/main.nf
The text was updated successfully, but these errors were encountered: