diff --git a/securesystemslib/formats.py b/securesystemslib/formats.py
index 0175fcea..a90becee 100755
--- a/securesystemslib/formats.py
+++ b/securesystemslib/formats.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   formats.py
diff --git a/securesystemslib/interface.py b/securesystemslib/interface.py
index 972e9076..3afb4fdd 100644
--- a/securesystemslib/interface.py
+++ b/securesystemslib/interface.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   interface.py
diff --git a/securesystemslib/keys.py b/securesystemslib/keys.py
index 76c37ab8..dc71198a 100755
--- a/securesystemslib/keys.py
+++ b/securesystemslib/keys.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   keys.py
diff --git a/securesystemslib/rsa_keys.py b/securesystemslib/rsa_keys.py
index 30be8b32..94c1a678 100755
--- a/securesystemslib/rsa_keys.py
+++ b/securesystemslib/rsa_keys.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   rsa_keys.py
diff --git a/securesystemslib/schema.py b/securesystemslib/schema.py
index eba7be7e..520cfa91 100755
--- a/securesystemslib/schema.py
+++ b/securesystemslib/schema.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   schema.py
diff --git a/securesystemslib/settings.py b/securesystemslib/settings.py
index 81692c26..0556e539 100755
--- a/securesystemslib/settings.py
+++ b/securesystemslib/settings.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   settings.py
diff --git a/tests/aggregate_tests.py b/tests/aggregate_tests.py
index 3a2939d0..f07c01b9 100755
--- a/tests/aggregate_tests.py
+++ b/tests/aggregate_tests.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   aggregate_tests.py
diff --git a/tests/check_gpg_available.py b/tests/check_gpg_available.py
index 8b95cc42..12b4b8ec 100644
--- a/tests/check_gpg_available.py
+++ b/tests/check_gpg_available.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   check_gpg_available.py
diff --git a/tests/check_kms_signers.py b/tests/check_kms_signers.py
index 236660cc..494fa583 100644
--- a/tests/check_kms_signers.py
+++ b/tests/check_kms_signers.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 This module confirms that signing using KMS keys works.
 
diff --git a/tests/check_public_interfaces.py b/tests/check_public_interfaces.py
index 9b195d20..0ff1ba7b 100644
--- a/tests/check_public_interfaces.py
+++ b/tests/check_public_interfaces.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   check_public_interfaces.py
diff --git a/tests/check_public_interfaces_gpg.py b/tests/check_public_interfaces_gpg.py
index eeadf18a..72de6d6b 100644
--- a/tests/check_public_interfaces_gpg.py
+++ b/tests/check_public_interfaces_gpg.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 """
 <Program Name>
   check_public_interfaces_gpg.py
diff --git a/tests/test_dsse.py b/tests/test_dsse.py
index a91893b4..909d9457 100644
--- a/tests/test_dsse.py
+++ b/tests/test_dsse.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """Test cases for "metadata.py". """
 
 import copy
diff --git a/tests/test_ecdsa_keys.py b/tests/test_ecdsa_keys.py
index 732d496e..cfe5049a 100755
--- a/tests/test_ecdsa_keys.py
+++ b/tests/test_ecdsa_keys.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env/ python
-
 """
 <Program Name>
   test_ecdsa_keys.py
diff --git a/tests/test_ed25519_keys.py b/tests/test_ed25519_keys.py
index 6b5134b8..f60ac1a7 100755
--- a/tests/test_ed25519_keys.py
+++ b/tests/test_ed25519_keys.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env/ python
-
 """
 <Program Name>
   test_ed25519_keys.py
diff --git a/tests/test_exceptions.py b/tests/test_exceptions.py
index cb227219..3f3af413 100755
--- a/tests/test_exceptions.py
+++ b/tests/test_exceptions.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   test_exceptions.py
diff --git a/tests/test_formats.py b/tests/test_formats.py
index f752382c..7b9919cf 100755
--- a/tests/test_formats.py
+++ b/tests/test_formats.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   test_formats.py
diff --git a/tests/test_gpg.py b/tests/test_gpg.py
index 5e443036..0a72d781 100644
--- a/tests/test_gpg.py
+++ b/tests/test_gpg.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   test_gpg.py
diff --git a/tests/test_hash.py b/tests/test_hash.py
index 647c7e04..04f7654d 100755
--- a/tests/test_hash.py
+++ b/tests/test_hash.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   test_hash.py
diff --git a/tests/test_hsm_signer.py b/tests/test_hsm_signer.py
index 00a4b8e9..5a01f0ed 100644
--- a/tests/test_hsm_signer.py
+++ b/tests/test_hsm_signer.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 """Test HSMSigner
 """
 import os
diff --git a/tests/test_interface.py b/tests/test_interface.py
index 00e4f911..b2b85cb1 100755
--- a/tests/test_interface.py
+++ b/tests/test_interface.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   test_interface.py
diff --git a/tests/test_keys.py b/tests/test_keys.py
index 03dccbc7..b550197c 100755
--- a/tests/test_keys.py
+++ b/tests/test_keys.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   test_keys.py
diff --git a/tests/test_rsa_keys.py b/tests/test_rsa_keys.py
index 0008e030..03750dd2 100755
--- a/tests/test_rsa_keys.py
+++ b/tests/test_rsa_keys.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   test_rsa_keys.py
diff --git a/tests/test_schema.py b/tests/test_schema.py
index ede75251..b91f79b3 100755
--- a/tests/test_schema.py
+++ b/tests/test_schema.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   test_schema.py
diff --git a/tests/test_signer.py b/tests/test_signer.py
index 23b6761c..126cd566 100644
--- a/tests/test_signer.py
+++ b/tests/test_signer.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """Test cases for "signer.py". """
 
 import copy
diff --git a/tests/test_util.py b/tests/test_util.py
index 3ce37223..d94fb9bb 100644
--- a/tests/test_util.py
+++ b/tests/test_util.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python
-
 """
 <Program Name>
   test_util.py