diff --git a/packages/react/src/components/Menu/Menu.js b/packages/react/src/components/Menu/Menu.js
index 05fecfb64f00..cb2789f50932 100644
--- a/packages/react/src/components/Menu/Menu.js
+++ b/packages/react/src/components/Menu/Menu.js
@@ -22,6 +22,7 @@ import { useMergedRefs } from '../../internal/useMergedRefs';
 import { usePrefix } from '../../internal/usePrefix';
 
 import { MenuContext, menuReducer } from './MenuContext';
+import { useLayoutDirection } from '../LayoutDirection';
 
 const spacing = 8; // distance to keep to window edges, in px
 
@@ -71,6 +72,9 @@ const Menu = React.forwardRef(function Menu(
     (item) => !item.disabled && item.ref.current
   );
 
+  // Set RTL based on document direction or `LayoutDirection`
+  const { direction } = useLayoutDirection();
+
   function returnFocus() {
     if (focusReturn.current) {
       focusReturn.current.focus();
@@ -82,8 +86,18 @@ const Menu = React.forwardRef(function Menu(
       focusReturn.current = document.activeElement;
 
       const pos = calculatePosition();
-      menu.current.style.left = `${pos[0]}px`;
-      menu.current.style.top = `${pos[1]}px`;
+      if (
+        (document?.dir === 'rtl' || direction === 'rtl') &&
+        !rest?.id?.includes('MenuButton')
+      ) {
+        menu.current.style.insetInlineStart = `initial`;
+        menu.current.style.insetInlineEnd = `${pos[0]}px`;
+      } else {
+        menu.current.style.insetInlineStart = `${pos[0]}px`;
+        menu.current.style.insetInlineEnd = `initial`;
+      }
+
+      menu.current.style.insetBlockStart = `${pos[1]}px`;
       setPosition(pos);
 
       menu.current.focus();
diff --git a/packages/react/src/components/Menu/Menu.stories.js b/packages/react/src/components/Menu/Menu.stories.js
index 103e7078bc68..b68d24eaba1d 100644
--- a/packages/react/src/components/Menu/Menu.stories.js
+++ b/packages/react/src/components/Menu/Menu.stories.js
@@ -43,7 +43,7 @@ export const Playground = (args) => {
   const target = document.getElementById('storybook-root');
 
   return (
-    <Menu {...args} target={target}>
+    <Menu {...args} target={target} x={document?.dir === 'rtl' ? 250 : 0}>
       <MenuItem label="Share with">
         <MenuItemRadioGroup
           label="Share with"
diff --git a/packages/react/src/components/Menu/MenuItem.js b/packages/react/src/components/Menu/MenuItem.js
index c241a7ebca54..3ee31f247a29 100644
--- a/packages/react/src/components/Menu/MenuItem.js
+++ b/packages/react/src/components/Menu/MenuItem.js
@@ -9,7 +9,7 @@ import cx from 'classnames';
 import PropTypes from 'prop-types';
 import React, { useContext, useEffect, useRef, useState } from 'react';
 
-import { CaretRight, Checkmark } from '@carbon/react/icons';
+import { CaretRight, CaretLeft, Checkmark } from '@carbon/react/icons';
 import { keys, match } from '../../internal/keyboard';
 import { useControllableState } from '../../internal/useControllableState';
 import { useMergedRefs } from '../../internal/useMergedRefs';
@@ -17,6 +17,7 @@ import { usePrefix } from '../../internal/usePrefix';
 
 import { Menu } from './Menu';
 import { MenuContext } from './MenuContext';
+import { useLayoutDirection } from '../LayoutDirection';
 
 const hoverIntentDelay = 150; // in ms
 
@@ -40,6 +41,7 @@ const MenuItem = React.forwardRef(function MenuItem(
   const menuItem = useRef();
   const ref = useMergedRefs([forwardRef, menuItem]);
   const [boundaries, setBoundaries] = useState({ x: -1, y: -1 });
+  const [isRtl, setRtl] = useState(false);
 
   const hasChildren = Boolean(children);
   const [submenuOpen, setSubmenuOpen] = useState(false);
@@ -60,10 +62,17 @@ const MenuItem = React.forwardRef(function MenuItem(
 
   function openSubmenu() {
     const { x, y, width, height } = menuItem.current.getBoundingClientRect();
-    setBoundaries({
-      x: [x, x + width],
-      y: [y, y + height],
-    });
+    if (isRtl) {
+      setBoundaries({
+        x: [-x, x - width],
+        y: [y, y + height],
+      });
+    } else {
+      setBoundaries({
+        x: [x, x + width],
+        y: [y, y + height],
+      });
+    }
 
     setSubmenuOpen(true);
   }
@@ -126,6 +135,16 @@ const MenuItem = React.forwardRef(function MenuItem(
     // eslint-disable-next-line react-hooks/exhaustive-deps
   }, []);
 
+  // Set RTL based on document direction or `LayoutDirection`
+  const { direction } = useLayoutDirection();
+  useEffect(() => {
+    if (document?.dir === 'rtl' || direction === 'rtl') {
+      setRtl(true);
+    } else {
+      setRtl(false);
+    }
+  }, [direction]);
+
   return (
     <li
       role="menuitem"
@@ -150,7 +169,7 @@ const MenuItem = React.forwardRef(function MenuItem(
       {hasChildren && (
         <>
           <div className={`${prefix}--menu-item__shortcut`}>
-            <CaretRight />
+            {isRtl ? <CaretLeft /> : <CaretRight />}
           </div>
           <Menu
             label={label}
diff --git a/packages/react/src/components/MenuButton/index.js b/packages/react/src/components/MenuButton/index.js
index dc364c710668..36238888373b 100644
--- a/packages/react/src/components/MenuButton/index.js
+++ b/packages/react/src/components/MenuButton/index.js
@@ -59,7 +59,7 @@ const MenuButton = React.forwardRef(function MenuButton(
   }
 
   function handleOpen() {
-    menuRef.current.style.width = `${width}px`;
+    menuRef.current.style.inlineSize = `${width}px`;
   }
 
   const triggerClasses = classNames(`${prefix}--menu-button__trigger`, {