diff --git a/browser/net/global_privacy_control_network_delegate_helper_browsertest.cc b/browser/net/global_privacy_control_network_delegate_helper_browsertest.cc
new file mode 100644
index 000000000000..fefa8f518a10
--- /dev/null
+++ b/browser/net/global_privacy_control_network_delegate_helper_browsertest.cc
@@ -0,0 +1,126 @@
+/* Copyright (c) 2020 The Brave Authors. All rights reserved.
+ * This Source Code Form is subject to the terms of the Mozilla Public
+ * License, v. 2.0. If a copy of the MPL was not distributed with this file,
+ * You can obtain one at https://mozilla.org/MPL/2.0/. */
+
+#include "brave/common/brave_features.h"
+#include "brave/common/network_constants.h"
+#include "chrome/browser/ui/browser.h"
+#include "chrome/test/base/in_process_browser_test.h"
+#include "chrome/test/base/ui_test_utils.h"
+#include "components/network_session_configurator/common/network_switches.h"
+#include "content/public/test/browser_test.h"
+#include "content/public/test/browser_test_utils.h"
+#include "content/public/test/test_utils.h"
+#include "net/dns/mock_host_resolver.h"
+#include "net/test/embedded_test_server/http_request.h"
+
+enum class GPCHeaderResult {
+  kOk,
+  kNoRequest,
+  kNoHeader,
+  kWrongValue,
+};
+
+class GlobalPrivacyControlNetworkDelegateBrowserTest
+    : public InProcessBrowserTest {
+ public:
+  GlobalPrivacyControlNetworkDelegateBrowserTest()
+      : https_server_(net::EmbeddedTestServer::TYPE_HTTPS) {
+    feature_list_.InitAndEnableFeature(features::kGlobalPrivacyControl);
+  }
+
+  void SetUpOnMainThread() override {
+    InProcessBrowserTest::SetUpOnMainThread();
+
+    header_result_ = GPCHeaderResult::kNoRequest;
+
+    host_resolver()->AddRule("*", "127.0.0.1");
+
+    https_server_.RegisterRequestMonitor(base::BindRepeating(
+        &GlobalPrivacyControlNetworkDelegateBrowserTest::HandleRequest,
+        base::Unretained(this)));
+
+    ASSERT_TRUE(https_server_.Start());
+  }
+
+  void HandleRequest(const net::test_server::HttpRequest& request) {
+    base::AutoLock auto_lock(header_result_lock_);
+
+    auto it = request.headers.find(kSecGpcHeader);
+    if (it == request.headers.end()) {
+      header_result_ = GPCHeaderResult::kNoHeader;
+    } else if (it ->second != "1") {
+      header_result_ = GPCHeaderResult::kWrongValue;
+    } else {
+      header_result_ = GPCHeaderResult::kOk;
+    }
+  }
+
+  void SetUpCommandLine(base::CommandLine* command_line) override {
+    InProcessBrowserTest::SetUpCommandLine(command_line);
+    // Allows the embedded test server to serve HTTPS
+    command_line->AppendSwitch(switches::kIgnoreCertificateErrors);
+  }
+
+  const net::EmbeddedTestServer& https_server() { return https_server_; }
+
+  GPCHeaderResult header_result() {
+    base::AutoLock auto_lock(header_result_lock_);
+    return header_result_;
+  }
+
+ protected:
+  base::test::ScopedFeatureList feature_list_;
+
+ private:
+  net::test_server::EmbeddedTestServer https_server_;
+  mutable base::Lock header_result_lock_;
+  GPCHeaderResult header_result_;
+};
+
+// When kGlobalPrivacyControl is enabled, the Sec-GPC flag should appear on
+// request headers.
+IN_PROC_BROWSER_TEST_F(GlobalPrivacyControlNetworkDelegateBrowserTest,
+                       IncludesSecGPCHeader) {
+  const GURL target = https_server().GetURL("example.com", "/index.html");
+  ui_test_utils::NavigateToURL(browser(), target);
+  EXPECT_EQ(header_result(), GPCHeaderResult::kOk);
+}
+
+// The Global Privacy Control spec also defines the
+// `navigator.globalPrivacyControl` JS property, which is read-only. In Brave
+// it will always return `true`.
+IN_PROC_BROWSER_TEST_F(GlobalPrivacyControlNetworkDelegateBrowserTest,
+                       NavigatorGlobalPrivacyAPI) {
+  const GURL target = https_server().GetURL("example.com", "/index.html");
+  ui_test_utils::NavigateToURL(browser(), target);
+
+  auto* rfh =
+      browser()->tab_strip_model()->GetActiveWebContents()->GetMainFrame();
+
+  EXPECT_EQ(true, content::EvalJs(rfh, "navigator.globalPrivacyControl"));
+  EXPECT_EQ(true, content::EvalJs(rfh,
+                                  "(function() {"
+                                  "  navigator.globalPrivacyControl = false;"
+                                  "  return navigator.globalPrivacyControl;"
+                                  "})()"));
+}
+
+class DisabledGlobalPrivacyControlNetworkDelegateBrowserTest
+    : public GlobalPrivacyControlNetworkDelegateBrowserTest {
+ public:
+  DisabledGlobalPrivacyControlNetworkDelegateBrowserTest() {
+    feature_list_.Reset();
+    feature_list_.InitAndDisableFeature(features::kGlobalPrivacyControl);
+  }
+};
+
+// When kGlobalPrivacyControl is disabled, the Sec-GPC flag should not appear
+// on request headers.
+IN_PROC_BROWSER_TEST_F(DisabledGlobalPrivacyControlNetworkDelegateBrowserTest,
+                       ExcludesSecGPCHeader) {
+  const GURL target = https_server().GetURL("example.com", "/index.html");
+  ui_test_utils::NavigateToURL(browser(), target);
+  EXPECT_EQ(header_result(), GPCHeaderResult::kNoHeader);
+}
diff --git a/test/BUILD.gn b/test/BUILD.gn
index 8e2e6bc0a4b3..8f41e3df358a 100644
--- a/test/BUILD.gn
+++ b/test/BUILD.gn
@@ -531,6 +531,7 @@ test("brave_browser_tests") {
     "//brave/browser/net/brave_network_delegate_hsts_fingerprinting_browsertest.cc",
     "//brave/browser/net/brave_site_hacks_network_delegate_helper_browsertest.cc",
     "//brave/browser/net/brave_system_request_handler_browsertest.cc",
+    "//brave/browser/net/global_privacy_control_network_delegate_helper_browsertest.cc",
     "//brave/browser/policy/brave_policy_browsertest.cc",
     "//brave/browser/profiles/brave_bookmark_model_loaded_observer_browsertest.cc",
     "//brave/browser/profiles/brave_profile_manager_browsertest.cc",